Ua hoʻokuʻu maikaʻiʻo Iphise me C57Bl / 6 Mouse Primary Hepatocyte

C57bl / 6, i kapaʻiaʻo ia e like me ka "c57 black 6", "C57", a iʻole "COCBED 6" Ua hoʻohana nuiʻia ia ma keʻano he misgegenic cones i ka hana gentic e hōʻike ana i nā hemahema Genetic kanaka. He mea nui kekahi o ka nui loa i hoʻohana nuiʻia a maikaʻi loa ia?

Ⅰ. Kumu a me nā hiʻohiʻona o ka c57bl / 6 mau mice

I ka makahiki 1921, C.C. Liʻiliʻi ka mea liʻiliʻi i ka hewa o ka soy obby e hana i nā poʻe i hoʻopaʻaʻia i nā holoholona hihiu; Ua hakuʻia ka wahine No. 57 me ka A. 52 Ma ke kāne e loaʻa ai ka C57. Ua hoʻoponoponoʻo ia i ke kala hulu manu o ka c57 a i kapaʻiaʻo C57br (Brown E like me ka nui o nā camplines he nui o nā c57 mau mice, c.c. ʻO ka liʻiliʻi liʻiliʻi i lalo o ka laina i hoʻohanaʻia 6 ma 1937, ua kūʻaiʻiaʻo ia he c57bl / 6.

Ua manaʻoʻia he "maʻamau" maʻamau "inbred strain, ka c57bl / 6 mouse i hāʻawi i nāʻano genetic no nāʻano genetic

Eia kekahi mea hoʻohui c57bl / 6 e lawelawe ana ma keʻano he hoʻohālike wā kiʻekiʻe me ka hopena kiʻekiʻe, a me nā hua'ōlelo hoʻohālikelike. Ma ka hopena o ka hopena, c57bl / 6 lilo nō ma Bilume mua,ʻo nā Polokalamu Kauoha, amenurology, piliMobion, Hoʻohui; Ua hoʻohanaʻiaʻo ia no ke aʻoʻana i nā maʻi a me nā maʻi no ka maʻi a me nā maʻi maʻi maʻi.

Ⅱ. Hoʻohālikelike o C57Bl / 6 mau mode subtypes

I ka makahiki 1947, ua hōʻikeʻo Jackson i ka c57bl / 6 mai ka liʻiliʻi a me ka inoaʻo C57bl / 6j. I ka makahiki 1951, National Institute of Health (nih) i hōʻikeʻia iā c57bl / 6j 32nd no subline c57bl / 6n mai jackn / 6n mai jackson laboratory. E like me ka nui o nā meaʻokoʻa ma waena o nā subslines, pono e koho i ka strac mouse kūpono e like me nā kumu hoʻokolohua.

Eia kekahi, i ka manawa c57bl / 6j a me C57Bl / 6n i kohoʻia e nā hiʻohiʻona holoholona ma waena o lākou.

Ⅲ. ʻO ka mea nui o C57BL / 6 mau kiʻi kiʻi kiʻi kiʻi kiʻi

ʻO nā hepatoclers hepatocytes he mau'āpana pale o ka paleʻana i nā'āpana o nā holoholona holoholona mai nā holoholona holoholona

Ke kūlike mau nei nā hepatocytes hepatocytes me ka pūnaewele Vivo, a me nā mea hoʻokūkū o nā ENTZYMES a me nā mea noiʻi i ka pono o ka lāʻau lapaʻau. ʻO kaʻike,ʻo ka hoʻohanaʻana i nā hepatika mua no nā mea noiʻi vitroʻaʻole ia e hana i nā pilikia o nā holoholona holoholona, ​​a hōʻemi i ke kumu o nā hoʻokolohua holoholona. E like me kēia, ua hoʻohana nuiʻia nā hepatocytes mua ma Molecular a me Clielular Biology a me nā mea noiʻi maʻamau, e like me nā mea palekana a me nā geneomics a me nā geneomics I ka manawa like, hiki iā lākou ke hoʻohanaʻia i loko o kēiaʻoihana kaulana loa, e like me ka loiloi lāʻau lapaʻau, nā mea kūʻai aku i ka lāʻau lapaʻau nui a me keʻano o ka mea kūʻai aku.

C57BL / 6 mau mice, e like me keʻano hana hoʻohālike maʻamau, loaʻa nā waiwai maikaʻi loa. No laila,ʻo ke aʻoʻana a me ka hoʻomohalaʻana o kā lākou mea nui he nui o nā hepatocytes koʻikoʻi no ka mālamaʻana i ka multidisciplary.

Ⅳ. Ipea -Pau huahana e pili ana

Ke pane nei i kēiaʻano hana, Iphise, he alakaʻi i ke alakaʻiʻana i nā mea kūʻai aku no ka hoʻohanaʻana i nā mea kūʻai aku

Ua hoʻokaʻawaleʻia nāʻenehana IPhie i ka hoʻopiʻiʻana i ka hepatoclent i ka hoʻopiʻiʻana mai ke kāne c57bl / 6 moice a ho'āʻoʻia i ka hana o ka lāʻau lapaʻau lāʻau lapaʻau lāʻau lapaʻau. Ua hoʻohana nāʻenehana i kahiʻano hope loa o ka 1 μm Verapamil e like me ka mea e hoʻoponopono ai i keʻano o ka lāʻau lapaʻau ma 0 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, a me nā miny, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min, 15 min Ua hoʻopau lākou i ka hapalua -ʻO ke ola o Vrapamil ma 28.51 ML / MIN / MITRL / MIN / 6 MITRL / 6 MITL / 6 MITLL / 6 MITLLY.

Kamana

Loaʻa nā huahana IPase mai nā kumuwaiwai a me kaʻike maopopoʻia.

Palekana

Ua ho'āʻoʻia nā holoholona no nā mea waiwai e hōʻoia ai e hōʻoia i ka palekana o ka maikaʻi o ka huahana.

Hana kiʻekiʻe

Ua hōʻoia kaʻenehana IPhin i ka hana o ka hana o ke cyp450 e hoʻohana ana i ka verapamil ma keʻano he palena o ka mea kūʻai aku.

Ke ola hou kiʻekiʻe

Hiki i ka helu hoʻihoʻi o ka cryoprresertation hiki keʻoi aku ma mua o 90%.

Nā'umehana

Hiki i ka Iphise ke hāʻawi i ka lawelawe i hanaʻia no nāʻano kūikawā a me nā'āpana e like me nā pono o nā mea kūʻai aku.

Ma kahi o nā hana hou loa i hoʻopauʻiaʻo C57Bl / 6 Mouse Hepanent Hiki i ka Iphise ke hāʻawi i nā mea kūʻai aku me nāʻano likeʻole o ka genera a me nā kiko'ī o nā hepatika kumu e kōkua ai i nā mea kūʻai aku a me nā hoʻokolokolo.