ʻO keʻano o ke koko comipheral kanaka moni (pbmc)
Hiki i ka PBMC ke hiki i ka MIMIC i loko o - VIVO CHANCO e hāʻawi i kahi kūpono ma - VITRO NORT no ka hoʻolālāʻana o ka lāʻau lapaʻau. E aʻo ana i nā hopena o nā lāʻau lapaʻau ma nā kau i nā pae a me nā hōʻailona hōʻailona o nā PBMcs ke kōkua i nā manaʻo lāʻauʻole a me nā kuhikuhi no kaʻikeʻoleʻana a me ka hoʻomohalaʻana.
ʻO nā'ōlelo kuhikuhi mua o PBMC ma IMMunooTheperatuunitic i hoʻohanaʻia i ka hoʻomohala lāʻau lapaʻau.
1.tanibody - Kaniki Calleget - Pūnaewele Cytotoxicity (AdCC): PBMC e pāʻani ana i kahi kī nui ma Adcc; NK Celells, Macrophages, Etc.ʻO ka mea nui o nā pbmcs nui i ka hana adcc. Hiki i kēia mau pahu ke pepehi i nā mea i hoʻopiliʻia e nā cell i hoʻonuiʻia e nā antibodies ma o ka hoʻopaʻaʻana iā lākou.
2.Mixed Lymphocyte reaction (MLR): He CO -ʻO ka'ōnaehana moʻomeheu no nā cell mua a me nā cell i hiki ke hoʻohanaʻia. ʻO DC cell ka hōʻike nui o PD - L1 i ko lākou wahi, e hiki ke hoʻopaʻaʻia a me ka papa o nā cell. Inā hiki i ka lāʻau lapaʻau ke pale i ke ala ākea ma waena o nāʻelua, a laila e hiki ke hoʻoikaika i ka mana o nā TE CLELD a pēlā e hōʻoia ai i ka hana hana o ka lāʻau lapaʻau.
3.t nā mea hana hana cell cell Ma nā mea kūʻai aku o ka cell i loaʻa, i loko o - nā mea kanu vitro e hoʻohana pinepineʻia a kūpono paha, e like me nā antigens a iʻole he antibodies a iʻole e like me nā antiries a iʻole he antibodies a iʻole nā antibodies.
4.T CHELL PROLLIKES ASSAY: E hoʻohana i ka waiwai o ka Stellay Stippy no ka mālamaʻana i nā hui o nā lāʻau lapaʻau ma nāʻoihana lāʻau lapaʻau ma nāʻoihanaʻoihana.
Ua hoʻokaʻawaleʻia nā pbmcs kanaka i hanaʻia e IPHES mai ke koko peripheral kanaka e ke koko o ka cedity gradient cedity. ʻO kēia mau pahu kīwī hoʻokahi nui o nā lymphocytes (t cell, b cell, a me nā monocytes.
▞ Nā meaʻike:
|
Inoa |
Mea no. |
Kaoaohai |
Kūlana o ka cell |
Kākau / hoʻouna |
|
ʻO nā cell monipheral kanaka moni |
082a01.11 |
5 miliona mau helu / ml |
Hou |
2 - 8˚CC mālama, ka laweʻana i ka pahu hopu |
▞ Ka palapala palapala:
I loko o - Vitro metabolism e aʻo ana i nā lāʻau lapaʻau.
