Nā hepatocytes hepatointtes Vuspebled hepatocytes: nāʻokoʻa kīwaha: nāʻokoʻa kīwī a me nā noi i ka ho'āʻoʻana i ka lāʻau lapaʻau

I. Ka Palapala

I ka hoʻolālāʻana o ka lāʻau lapaʻau a me nā noiʻi hepatocytes I waena o lākou,papatotes aʻO nā hepatocytes pale E mālama i nā hana likeʻole, e hana ana i ke koho ma waena o lākou ma ka pololei no ka pololei o Vitro Studies. Ka hoʻomaopopoʻana i nāʻokoʻa i loko o kā lākou mau hiʻohiʻona a me nā noi e kōkua i nā mea noiʻi e koho i ka hoʻohālike kūpono loa no kā lākou mau pono.

Iphise biosciences, he alakaʻi i nā hoʻoponopono houʻana i nā mea hoʻonaninani hou, kākoʻo i nā meaʻepekemaʻepekema ma ke hāʻawiʻana i nā noi kiʻekiʻe. Me ka loea o cell - nā mea kōkua i piliʻia a me nā metabolisiss

Hōʻike kēiaʻatikala i nāʻokoʻa koʻikoʻi ma waena o nā hepatokescal a me ka hoʻopiʻiʻana, ko lākou noi kūikawā i ka hoʻokolohua kūpono no kāu noiʻi.

II. He aha nā hepatocytes posteble?

ʻO nā hepatointtes hepatokytes i nā cells ola i loaʻa i ka hiki ke hoʻopili i kahi papa a hana i nā mea monolayers, e hoʻoikaika iā lākou no ka lōʻihi - Termlelo Vitro.

Nā hiʻohiʻona koʻikoʻi:

• Hiki ke hoʻopili i nā papa moʻomeheu

Mālama i ka Lie Liver - Nā Hana Kūʻai Kūʻai ma luna o nā manawa lōʻihi

Kūpono no ke aʻoʻana i ka invyme intjyme, i ka hanaʻana, a me ka hana laweʻana

Nā noi i ka ho'āʻoʻana i ka lāʻau lapaʻau:

• Long - Term Workus Metabolism Studies:Ua hoʻohanaʻia e loiloi i ke kaʻina o nā lāʻau lapaʻau i ka manawa.

• Nā Kūlana Kūʻai Kūʻai:ʻO ka loiloiʻana i ka hanaʻana o nā lāʻau lapaʻau i ka hana enzyme

• EPATOTICKYXICY SCORDER:Ka hoʻomaopopoʻana i ka pōʻino o ke ola i hanaʻia e nā mea hoʻohui hou.

• Nā noiʻi hanaʻoihana:Ke aʻoʻana i ka lāʻau lapaʻau -ʻO nā lāʻau lapaʻau lāʻau a me nā mea hou aku i luna a me nā mīkini.

III. He aha nā hepatocytes suspension?

ʻO nā hepatocytes hepatocytes non - nā mea i hoʻohana muaʻia no nā cell i hoʻohana muaʻia no ka pōkole. Noho lākou i ka hana enzymatic akāʻaʻole e hoʻopili i nā papa lāʻau, e hana pono iā lākou no nā loiloi wikiwiki.

Nā hiʻohiʻona koʻikoʻi:

• Nor - AdHearent, mālamaʻia i loko o kahi moku'āina

• Kūpono no ka pōkole -ʻO ka lāʻau lapaʻau lāʻau lapaʻau metabolism

• Hāʻawi i nā lāʻau lapaʻau hilinaʻi a me nā hana e like me ka nui
  
  

Nā noi i ka ho'āʻoʻana i ka lāʻau lapaʻau:

• Pōkole -ʻO nā lāʻau lapaʻau lāʻau lapaʻau lāʻau metabolisism:Ke ana ana i nā uku hoʻomaʻemaʻe hoʻomaʻemaʻe.

• Nā Kiʻi Nui - Kaʻiʻana i ka Screening (HTS):Ka ho'āʻoʻana e ho'āʻo nui ana i nā mea hoʻohui lehulehu no ka kūpaʻa metabolic.

• ʻO ka hāʻawiʻana i ka hopena:Ka hoʻomaopopoʻana i nā ala enzyme e pili ana i ka lāʻau lapaʻau lāʻau lapaʻau.

• ʻO ka lāʻau lapaʻau -ʻO nā noi lāʻau lapaʻau:Loiloi i ka hoʻokūkū hoʻokūkū hoʻokūkū a me ka enzyme i ka hiki ke loaʻa.

IV. Nā meaʻokoʻa ma waena o nā hepatocytes i hoʻopaʻaʻia

Hōʻike kēia mauʻokoʻa i ka mea nui o ke kohoʻana i keʻano hepatocyte kūpono e pili ana i nā pahuhopu noi.

V. Nā noi i nā lāʻau lapaʻau e hōʻike ana a me ka noiʻi

Ua hoʻohana nuiʻia nā hepatocytesʻelua a me nā suspension Ka hoʻomaopopoʻana i kā lākou noi e hōʻoia i ka hoʻolālā hoʻokolohua maikaʻi loa.

2. ʻO nā lāʻau lapaʻau lāʻau a me nā lāʻau lapaʻau lāʻau lapaʻau (DMPK):

• Kākoʻo maikaʻiʻia nā hepatocytes i ka lōʻihi - Term Metabolic Consisies.

• Hiki i nā Hepatocytes hepatocytes e hiki ai i ka loiloi wikiwiki o nā ala metabolina.
  
  

Toxicology a me ka palepae hepatotika.

• Ua kōkua nā hepatocytes hepatocytes i nā lāʻau lapaʻau cluncle -ʻO ka hōʻeha o ka wai (dili).

• ʻO nā hepatocytes hepatocytes e nānā i ka cytototicity a me nā metabolic.
  
  

'Oha nā Phase I & Phase II Itaboliss Studies:

• Phase wau i nā hopena

• ʻO nā haʻawina a Phase II CHUMUGATION (GLUCURIONDATIONS, SULMITATIONS) E koi pinepineʻia)
  
  

ʻO ke komoʻana a me nā haʻawina i hoʻopaʻaʻia:

• ʻO nā hepatokics he nui nā hepatottes Cyp enzyme incording noiʻi.

• Hāʻawiʻo ia i nā hepatocytes e hāʻawi i nā meaʻike wikiwiki i loko o ka enzyme i ka hiki.
  
  

KA WELO - PRITPT TRANCE SCORTING:

• Hoʻopili i nā hepatocytes e hoʻomaʻamaʻa nui i ka nui - Scow Compund Scounting.

• E kōkua i nā hepatocytes hepatocytes i ke kōkua i nā papa hana kiko'ī.

VI. Pehea e koho ai i keʻano hepatocyte kūpono no kāu noiʻi

Ke koho nei i nā hepatocytes no ka noiʻi, pono kekahi mau mea i noʻonoʻoʻia:

E aʻo i ka lōʻihi:

• Long - E koi i nā hoʻokolohua kiʻekiʻe e koi i nā hepatocytes i kohoʻia.

• Pōkole -ʻO Term metabolism asseays esomes i nā hepatocytes suspension.
  
  

Nā pahuhopu hoʻokolohua:

• Hoʻopiliʻia,ʻo ka hōʻeha, a me nā haumāna lawe:Nā kupeta patable.

• ʻO ka nānāʻana a me ka papa kuhikuhi a me nā hōʻoiaʻana i nā mea waiwai:Nā kuʻekuʻe i kāpili.
  
  

Nā koho koho:

• Hewatocytes kanakano ka piliʻana i ka pilina.

• Nā holoholona holoholonaNo nā haʻawina preclinical.
  
  

ʻO New Vet Vesopres i nā hepatocytes:

• He hepatocytes houmālama pono i ka pono kiʻekiʻe akā ua loaʻa ka loaʻa kālā.

• ʻO nā hepatocytes crypleretedHāʻawi i kaʻoluʻolu a me ka hana hou.
  
  

Hāʻawiʻo Iphise bioscise High - kiʻekiʻe kiʻekiʻe a me ka hoʻopiʻiʻana i nā hepatocytes no nā noi noiʻi. Mālama ko mākou loea i ka loaʻaʻana o nā meaʻepekema i nā reagents reiable a hōʻoiaʻia e kākoʻo i kaʻikeʻana i ka lāʻau lapaʻau a me ka ho'āʻoʻana.

VII. Hopena

Ka hoʻomaopopoʻana i nāʻokoʻa ma waena o nā hepatocytes i hoʻopaʻaʻia a me nā hepatointtes hepatocytes no ke kohoʻana i ka hoʻohālike maikaʻi a me nā haʻawina koʻikoʻi. Hoʻopili maikaʻiʻia nā hepatocytes exepel i ka lōʻihi - nā hoʻokolohua huaʻai,ʻoiai ke kīʻana i nā hepatoction

ʻO Iphise biosciences Me nā mana nui R & D a me kahi pūnaewele kikowaena honua honua, e hōʻoia mākou i nā meaʻepekema i loaʻa i nā hiʻohiʻona hepatocyte no kaʻokiʻana.

No kaʻike hou aku ma ke kohoʻana i nā hepatte a me kāu noiʻi, e kipa i nā bioscien i kēia mau lā.