ʻO nā hepatocytes mua

ʻO nā hepatocytes mua

ʻO ke ola, e like me keʻano mua no ka hoʻolahaʻana o ka lāʻau lapaʻau, e pāʻani i kahi hana koʻikoʻi i nā kaʻina hana lāʻau lapaʻau a me nā kaʻina hana. Loaʻa i nā hepatocyts mua i ka nui o nā hiʻohiʻona piha a me keʻano o nā alaloa o ke alapine. No kēia kumu, ua manaʻo nuiʻia nā hepatocytes mua ma keʻano he gula gula no ka hanaʻana i nā mea noiʻi a me nā mea noiʻi. Hāʻawi kēiaʻatikala i kahiʻike o 2D a me 3D moʻomeheu i nāʻano moʻomeheu mua a me kā lākou noi i ka hoʻomohalaʻana i ka lāʻau lapaʻau.

Nā hua'ōlelo:ʻO nā hua'ōlelo hepatocytes, 2D kanu kanu, 3D māla, nā hui

1. 1. Hoʻokaʻawale o nā hepatocytes mua

ʻO ka hoʻokaʻawaleʻana o nā hepatocytes muaʻo ia ka hana koʻikoʻi i ka hoʻokumuʻana i nā hiʻohiʻona o Vitro ma Vitros I nā holoholona liʻiliʻi, hiki ke hoʻokōʻiaʻo Liver ma keʻano o ka pūnaewele Vein a iʻole ka nui o ka Vena Cava Cava ʻO kekahi mau kumu nui e hoʻokō i ka hana kūleʻa o nā hepatocytes:ʻO ka mea mua,ʻo ka colligease,ʻaʻole pono ke collagenise. ʻO ka lua,ʻo ka manawa o ka digestion he mea nui loa-ma lalo o ka - digestion a ma luna o - Hiki i ka digesttion ke hoʻopau i ka huapalapala hepatocyte a me ka vibility. ʻO ke kolu,ʻo keʻano ola e pono ke kūpono, e like me nā hepatocytes e like me ka hōʻeha o ka ischemic. ʻO ka mea ola i hoʻohana no ka hoʻomākaukauʻana hepatocyte e hoʻomaʻamaʻa wikiwiki i nā uku metabolic a pale i ka hypoxia hypoxia.

ʻO nā kūlana no nā hepatocytes mua i hoʻohanaʻia ma ka noiʻi lāʻau lapaʻau e like me kēia:

1. 2.ʻO ka moʻomeheu Hepatocyte 2d

Ke kumu hoʻohālike hepatocyte

Aia ia i ka lāʻau lapaʻau piha - Metabolizzing enzymes a me nā Clofactors, e kūpono ana no ke aʻoʻana i nā ala hoʻomaʻemaʻe hoʻomaʻemaʻeʻo Metabolic. Eia nō naʻe,ʻo ka hikiʻana o nā hepatocytes suspension a me nā hana o ka lāʻau lapaʻau. Hoʻohana pinepineʻia kēia hiʻohiʻona e koho i ka hoʻomaʻemaʻeʻana i nā lāʻau lapaʻau me nā haʻahaʻa kiʻekiʻe i nā kumukūʻai kiʻekiʻe. Ke emi iho nei ka helu o ka clearance ma mua o 20%,ʻaʻole hiki ke hoʻoholoʻia nā waiwai clearance. ʻO nā hepatocyte cuspedis kahiko -ʻO nā hiʻohiʻona Vitro metabolism no ke kauʻana i nā mea i hikiʻole ke wānana i nā huahana metabol. Hiki ke loaʻa kahiʻano relay relay i keʻano (kiʻi 1) e hoʻolōʻihi i ka manawa incubation i 20 mau hola a lōʻihi paha.

Ke noi:ʻO ka hana enzyme a me nā mea hoʻokūkū hoʻokūkū metabolic no nā lāʻau molena liʻiliʻi.

Kiʻi 1. Ho'ōla i ke kaʻina hana o ke kaʻina hana

Source: Hoʻopuka ka lāʻau lapaʻau lāʻau, 2012,40 (9): 1860-1865

ʻO ka hoʻohālike hepatocytes

Ua kanuʻia nā hepatocytes hepatocytes i kahi'ōnaehana 2d ma Collage - Nā papa moʻomeheu i kālaiʻia. Hōʻike nā hepatocytes i kahi morphology epithelial ʻO nā kiʻi paʻi o nā moʻomeheu hepatocyte monothyte monoyer e hoʻopili ai: 1 Nele i nāʻano hou e pili ana i nā wahi'ē aʻe (i.e., non - Hikiʻole ke hāʻawi i nā mea kanu ponoʻole a me nā mea hana maracrine e kākoʻo ai i nā hepatocytes e hoʻokō ai i kā lākou mau hana a me ka cerum filana

Nā noi:

1). ʻO ka loiloi o ka lāʻau lapaʻau -Hoʻopilikiaʻia kēia i ka hoʻohuiʻana o ka enzyme, enzyme i ka lāʻau, a me nā mea kālepa. ʻO ka mua, i ka wā e hana ai kahi lāʻau lapaʻau e like me kahi intuducer, hiki iā ia ke alakaʻi i ka hoʻonuiʻiaʻana o ka lāʻau lapaʻau - metabolinizing a me nā mea halihali. Hiki i nā mea hoʻoikaika ikaika ke hoʻopau i nāʻano he nui o nā genentple like, e like me ka nui phenoball ʻO ka lua, aia kekahi mauʻano - nāʻokoʻa kūikawā i ka pehea o nā hepatocytes e pane ai i nā mea indicers. No ka laʻana,ʻo ka gifampicin kahi mea hana kūpono no ke kanaka a me nā heitatisty hepatokes ʻO ka hope loa,ʻo ka hanaʻinoʻana o Suboptimal i ka hanaʻana i nā hepatocytes i kohoʻia e hoʻemi i ka hōʻikeʻana o nā p450s a me nā pane i hoʻonuiʻia. E like me ka hōʻikeʻana ma ke kiʻi 3, he mau hepatocytes ma lalo o nā hana haʻahaʻa haʻahaʻa e hōʻike i nā hana basp1a2, cyp3a4, a me ke cyp2a4 No laila, ponoʻo Hepatocytes i ke olakino kūpono ma kahiʻano kūpono kūpono e pono ai e loaʻa i kaʻike pili pono.

Kiʻi 2.ʻO ka pilina ma waena o ka paleʻana i ka nui o keʻano o ka cryopytes kanaka a me ka enzyme incouction

Source: Hoʻopuka i nāʻenehana i kēia manawa, 2010, 7: 188 - 368 - 198

2). ʻO kahi loiloi hepatotopa: Nānā i nā loli morphological ma lalo o kahi microscope māmā, e like me keʻano morphology ʻO kaʻike o ka hepatocyte necrosis necrosis No ke cytokine - Hoʻokomoʻiaʻo Cytotoxicity

3). "E hoʻihoʻi i keʻano" no ke aʻoʻana i ka hoʻomaʻamaʻa lohiʻana me ka courance courance courance a me nā metabolites:ʻO ka hana o ka lāʻau lapaʻau - Metabolinizing enzymes i nā hepatocytes e hoʻomaka ana e hōʻemi ma hope o 24 mau hola. Ma hope o ka hoʻopiliʻana i nā hepatocytes plateble me serum - manuahi manuahi i loaʻa i ka hoʻohuiʻana o ka nui no 24 mau hola hou (kiʻi 3).

Kiʻi 3.ʻO ke kaʻina hana e hoʻololi ai i ke kaʻina hana

Waiwai:ʻO ka lāʻau lapaʻau lāʻau lapaʻau, 2016, 10: 3: 3 - 15 -

3). E loiloi i ka UPTUL FETATE, EXTOCYTOSISS, EXOSOMALL SMAL, a me nā pilikia e loaʻa ana ma ka Vedit Dead AcID.

Sandwich Stumunitation Model

Ma Vitro, hiki ke kanuʻia hepatocytes ma waena o nā papaʻelua o ka collagen a iʻole matagol e hana hou i nā hana Vivo. ʻO nā hepatocytes i hoʻopaʻaʻia ma waena o nā papaʻelua o Gel - Clipgen (Curnwich Computy Eia kekahi,ʻo Hepatockings i loko o ka moʻomeheu sandwich ke hoʻihoʻi hou i ka polarity

Kiʻi 4.ʻO ka hōʻikeʻana o nā mea halihali i nā mea lawe i keʻano hepatoctel

Source: Paha Kino Ike Loaʻa nei, 2010, 7, 188 - - 198 - 198

Nā noi:

1. 1). E kuhi ana i nā biliry biliry.
2. 2). Ka loiloiʻana i ka hepatic a me ka biliary a me ka biliary a me nā exogenous compounds a me nā metabolites.
3. 3). ʻO kaʻikeʻana i ka hoʻomaʻemaʻeʻana ma ka metabolism a me nā mea halihali, a kūkulu i nā hiʻohiʻona printionicy -
4. 4). Ke aʻoʻana i ka hepatotikanii a me ka hāʻawiʻana i nā mīkini no ka lāʻau lapaʻau clinical -ʻehaʻeha i hōʻehaʻia. Hoʻopiliʻia nāʻikepili i nā hiʻohiʻona o nā'ōnaehana lāʻau lapaʻau e wānana i ka lāʻau lapaʻau maikaʻi -ʻO ka hōʻehaʻana i ka wai i loko o nā kānaka.
5.  
   1. 3.ʻO ka moʻomeheu hepatocyte 3d

6. I nā'ōnaehana waiwai 3D, he moʻomeheu nā hepatocytes ma kahiʻekolu. ʻO kēia mau'ōnaehana Sport e hoʻolaha nei i nā kelepona - Cille a me Cell -ʻO nā hui matrix, e hoʻihoʻi hou i nāʻano lāʻau lapaʻau Hiki ke hoʻohanaʻia nā moʻomeheu hepatocyte 3D e aʻo i ke ola - Nā hana kūikawā ma Vivo - E like me nā Pono Pono.

   Nā Kūlana SPHERORO

Ma o ka hanaʻana e like me ka moʻomeheu ultra - melemele haʻahaʻa, e kau ana i ka moʻomeheu o ka choreronal ʻO kekahi pono o ka moʻomeheu spheroidʻo ia kēlā me kēia sptheroid e koi wale i ka 1,330 - Ua hōʻikeʻia nā noiʻi houʻana i nā moʻomeheu SPHEROCYTE SPHEROCYTE no 5 mau manawa e mālamaʻia ai nā mea kanu. Eia naʻe,ʻoi aku ka nui o ke ola ma mua o ka hoʻohuiʻana o ka cell. ʻO kaʻaoʻao o Basolateral o nā hepatoctive i hui pū me ke koko,ʻoiai e kahe ana i keʻano o ka apical

Nā noi:

1. 1). E aʻo ana i ka hoʻomaʻamaʻa lohiʻana me ka hoʻomaʻemaʻeʻana i ka courance courance a me nā metabolites.
2. 2). He noiʻiʻo Hepatotikaticxtic.
3. 3). ʻO ka loiloi o ka cellulate uptake, exetortosis

Kiʻi 5.ʻO keʻano moʻomeheu spheroid

Nā hiʻohiʻona Liver Organids

ʻO ka weheweheʻana o kaʻaeʻana o nā ornetids no nā mea hana he 3d i loaʻa mai nā cete still, nā celist o ka micro e pili ana i ka microned Ua loaʻa iā Liver Organids e like me ka hoʻohālike o keʻano noiʻi o ke kanaka ola o ke kanaka.

Nā kumuwaiwai no ke kūkuluʻana i ke kūkuluʻana i ke kau

① PlurIPOPODATE STEM (PSCS):

EMbryonic Stoem Cult (escs) a me nā mea i hoʻopiliʻia ai nā mea nui Ma lalo o ka mana o nā mea hōʻailona hōʻailona,ʻokoʻa lākou i loko o ka hepatocyte - e like me nā cell me ka hana a me ka hana. Eia nō naʻe, loaʻa iā Liver Jernedies mai PSCS e loaʻa i lalo o nā mea epigenetic a me nā loli genetic, e hōʻike ana i nā loli chromosody.

Nā noi:

1. 1). Nā hiʻohiʻona maʻi genetic
2. 2). Nā hiʻohiʻona maʻi maʻi maʻi maʻi
3. 3). ʻO ka ho'āʻoʻana o ka lāʻau lapaʻau lāʻau lapaʻau

② Liver Tissue - Nā Mana Uila:ʻO kēia mau clalangiocytes a me nā Hepatocytes. ʻO kahi hepatoitint Hoʻohālikelike i ka PSC - Ua loaʻa iā Jancedids i loaʻa iā Tanties i loaʻa mai ka nui o nā mea kanu, a mālama i nā genetic a me nā genetic. Eia nō naʻe,ʻo ka lōʻihi - Term Prolifetative hiki i nā huiʻo Hepatocyte kanaka hepatocyte e hoʻohālikelikeʻia i nā hepatocytes fetal Kāleʻa i ka moʻomeheu hepatoclete hepatocles i nā'ōnaehana genedids

ʻO keʻano moʻomeheu Incubate i loko o kahi moʻomeheu cell incubator (37 ° C) no 15 mau minuke. Ma hope o ka hoʻoponoponoʻana, e hoʻohui i ka moʻomeheu moʻomeheu kūikawā. ʻO ka pauku ma hope o 14 mau lā. E hoʻololi i ka medium kumu me ka neʻe likeʻole ma hope o 7 - 10 mau lā.

Nā noi:

1. 1). Nā hiʻohiʻona hepatotopa
2. 2). Ma Vitro metabolism hoʻonāukiuki
3. 3). Non - inu momona momona momona
4. 4). Hoʻolālā ka lāʻau lapaʻau no nā maʻi no nā maʻi maʻi maikaʻiʻole

Kiʻi 6.ʻO ka moʻomeheu a me ke kaʻina hana o ka Tissue - Nā Minuti

Kumuhana: Opele & Bosificano (2023) 13: 197

Ka hoʻohālikelikeʻana i ka moʻomeheu spheroid a me ka moʻomeheu organid

4.ʻO ka hepatocyte mua c -ʻano moʻomeheu

1. 1. 2D kumu mua hepatocyte co -ʻano moʻomeheu
      

      I ka 2d prim pumel ponatocyte c - culture hoʻohālike,ʻelua aʻoi aku paha nāʻano kelepona likeʻole a me nāʻano likeʻole. ʻO ke kiʻi nui o kēiaʻano hoʻohālike e pili ana i keʻano ma waena o nāʻano kelepona likeʻole ma waena o nā cytoxal a me keʻano o nā cytoxal ʻO nā hana hepatocyte mua, e like me ka hana hou a me ka hanaʻana i ka lāʻau lapaʻau a me nā lāʻau lapaʻau lāʻau lapaʻau, hiki ke mālamaʻia iʻekolu mau pule.

      Nā noi:

      1. 1). ʻO nā hepatocles hepatocytes co - i hoʻohālikelikeʻia me nā fibroblasts: Hoʻohanaʻia kēia kumu hoʻohālike no ka hoʻopaʻaʻana i ka heluʻana o ka hōʻoia o ka hōʻoia -
      2. 2). ʻO nā hepatottes hepatoctive hepotottes co - nā mea aloha i nā mea ola a nā cheels.
      3. 3). ʻO nā hepatocles hepatocytes co - i hoʻohālikelikeʻia me nā cells: Hoʻohanaʻia kēiaʻano hoʻohālike eʻike i nā huapalapala o ka lāʻau lapaʻau.
      4. 4). ʻO Hephatem's Hepatomax ™CO -ʻO ka'ōnaehana moʻomeheu: Ua hoʻokumuʻiaʻo Iphuse i kahi'ōnaehana moʻomeheu me nā hepatinal™. ^. E CO - E noʻonoʻo ana i nā hepatocytes kanaka mua me nā cells stomal, hiki ke mālama i ka hana maikaʻi o ke kanaka no 3 mau pule. He kūpono kēia'ōnaehana no ka hoʻopaʻaʻana i ka lohiʻana i ka lohiʻana o ka cleanight cleanight a me ko lākou metabolites.

      Hāʻawi kēia CO -ʻO keʻano moʻomeheu moʻomeheu i loaʻa i kahi papa kiʻi kūpono no ka loiloiʻana i ka lāʻau lapaʻau e pili ana i keʻano o nā maʻi maʻi
      
      

      3d kumu kumuma co - nāʻano moʻomeheu

      Kuu 3D CO - E CLUNA: E pili ana kēia kumu hoʻohālike i nāʻanoʻelua a iʻole nāʻano hana likeʻole o nā chell. E pili pono i ka COCE 3D CO -ʻO ka moʻomeheu e pili ana i ka hoʻopiliʻana ma waena o nā cell overthems e like me nā cyomenika.

      Nā noi:

      1. 1). ʻO ke kumu hoʻohālike o Litr Fibrosis: Ua hoʻohanaʻia e aʻo i nā mīkini a me ka holomua o ka firrrosis.
      2. 2). ʻO ka lāʻau lapaʻau -ʻO ka hōʻehaʻana o ka wai.
      3. 3). ʻO nā lāʻau lapaʻau, ka lāʻau lapaʻau lāʻau lapaʻau, a me ka enzyme incouction: e loiloi ana i nā lāʻau lapaʻau e hoʻopili ai

      Kuhi manuahi 3D CO - moʻomeheu: Hoʻohana kēiaʻano hana i kahi'ōnaehana hoʻokaʻawale kino (e.g., transwell a iʻole nāʻano hana likeʻole a iʻole nā ​​mea e pili ana i nāʻano he 3d. ʻO ke kamaʻilio ma waena o nā cells e loaʻa ma o nā cytokines solluble.

      Nā noi:
      Hoʻohana no ke aʻoʻana no ke aʻoʻana i ke kamaʻilio ma waena o nā wahi ola i ke kino.

      
      I ka hōʻuluʻulu,ʻo Iphise, he alakaʻi i loko o vitro biological noiʻi, hāʻawi i nā hopena palekana non. Mai ke kaawale a me ka moʻomeheu o nā hepatocytes mua mai nāʻano hana e like me nā mea e pili ana i nāʻoihana e pili ana i nā mea kūʻai aku no ka lāʻau lapaʻau He hoa hana mākou no nā mea kūʻai aku i kaʻoihana lāʻau lapaʻau, loaʻa i kaʻokiʻana i kaʻokiʻana.