He aha nā pbmcs kanaka a me kā lākou hana i loko o ka umu?

Kāne pbmcs, a iʻole nā ​​cell colling mononnuclear chell, he hui koʻikoʻi o nā cell i ka nui o ke kaheʻana i loko o kou koko. Hoʻokomo kēia mau mea i nā lymphocytes, monocytes, a me nā condritic cells Mālamaʻo PBMcs iāʻoe ma kaʻikeʻana a me ka hanaʻoleʻana i nā pathogensʻino, e like me nā balala a me nā maʻi a me nā maʻi,ʻoiai e mālama ana i ka kaulike immune.

Hōʻike nā noiʻi noiʻi PBMcs ma mua o ka manawa, me nā kelepona ma luna o 85% ma ka nui o nā hiʻohiʻona. Hoʻohui hou, hiki i kā lākou'ōlelo goment ke hoʻoponopono koke i ka wikiwiki. No ka mea, hoʻonuiʻia ka'ōlelo a me ka'ōlelo a pau ma luna oʻekolu mau hola i loko o nā hola. Ua hōʻikeʻia kēlā me kēiaʻano i ko lākou mea nui i ka hoʻoponoponoʻana i nā pane paneʻole.

Ka hoʻonohonohoʻana o nā pbmcs kanaka

Lymphocytes: T cell, b cell, a me nk cell cell

ʻO Lymphocytes he mea nui o nā pbmcs kanaka. Hoʻokomo lākou i nā cells T ELLS E kōkua i ka hoʻoponoponoʻana i nā pane nui a me nā mea e hoʻouka ai i nā cell. B cell i hana i nā antibodies, e pili pono ana i nā pathogensʻino. NK Celells, ma ka lima'ē aʻe, e nānā a luku a luku i nā kīʻaha abnormal, e like me nā maʻi maʻi a iʻole nā ​​hopena cell.

Hoihoi hou i kekahi mau lymphocytes ma nā pbmcs kanaka, iʻikeʻia ma ka cd3 + cd19 + cellib dual. Hiki i kēia mau mea hana ke hana e like me nā cell a me nā cell a me nā cell. Ua pane lākou i nā hoʻoweliweli ma o t - cell resptor (tcr) a me b - nā mea hōʻailona hōʻailona hōʻailona (BCR). Hiki i kēia Role keʻae iā lākou e komo i loko o nā pane a me nā pane. ʻO kahi laʻana, ua hoʻopili nui lākou i nā antigien ma mua o nā cell conventional Bel a me nā kumuwaiwai e like me nā cell.

Nā Monocytes a me kā lākou hanaʻana

ʻO nā monocytes kekahi mau mea nui i loko o nā pbmcs kanaka. ʻO kēia mau mea kūʻai aku i kāu kīʻaha koko, eʻimi ana i nā hōʻailona o ka maʻi a me nā meaʻino paha. Keʻike nei lākou i kahi pilikia,ʻaʻole lākou e neʻe i ka wahi i hoʻopilikiaʻia a hoʻololi i nā macrophages a iʻole nā ​​cell dendritic cell. ʻO Macrophages engulf a me nā pathogens a me nā mea kūʻai aku,ʻoiaiʻo nā mea hana dendritic i hōʻikeʻia i nā antigens i nāʻano'ē aʻe.

ʻO nā monocytes e hoʻokuʻu i nā cytokines, kahi e hōʻailona ai i nā molena e kōkua i ka hoʻoponoponoʻana i ka paneʻana i ka pane immune. Ma ka hanaʻana, ke hōʻoia nei lākou e lanakila maikaʻi ana kou kino i nā maʻi a iʻole nā ​​hōʻeha.

Nā Kūlana Dendritic a me kā lākou hana i ka hōʻike antiigen

ʻO nā cendritic cells he mea akamai antigen - Hōʻike i nā papa hana (APCS) i loko o nā PBMcs kanaka. Ke pāʻani nei lākou i kahi hana koʻikoʻi i loko o ka hoʻouluʻana i nā cell e hōʻike ana i ka hōʻikeʻana i nā antigens ma ko lākou wahi. Hōʻike ka noiʻi i nā cell dendritic wale nō ka hiki ke hana i nā apcs wale nō e hana ai i ka cd4 + a me cd8 + naïve t cell. ʻO kā lākou pono e hiki mai ana i ko lākou hiki ke hoʻolōʻihi i ka antigen antigen, e hoʻonui ana i ka loaʻaʻana o nā peptides no MHC FIREDER.

Hoʻomaopopo kēia mau pale i kaʻikeʻana o kāu'ōnaehana i ka hana a me ka paneʻana i nā hoʻoweliweli maikaʻi loa, e hana ana iā lākou i nā mea alohaʻole.

Hoʻomoʻaʻia o nā PBMcs kanaka

Nā kumuwaiwai o PBMcs:ʻO ke koko peripheral a me nā iwi iwi

Hiki ke hoʻokaʻawaleʻia nā pbmcs kanaka mai nā kumuwaiwaiʻelua ʻO ke koko kokoʻo ia ka mea maʻamau ma muli o kona komoʻana a me ka neʻeʻana o ka minima. ʻO ka iwiʻeleʻele, ma kaʻaoʻao'ē aʻe, hāʻawi i kahi kumu waiwai no nā cells nā mea kūʻai aku akā pono ke hana hou i ke kaʻina hana hou.

Hiki i ka hua a me ka hoʻomaʻemaʻe o nā pbmcs ke loli i keʻano o ke kumu a me keʻano. No ka laʻana, e hōʻike ana i nā haʻawina ficoll e hoʻokō ai i kahiʻano kiʻekiʻe a me ka hoʻomaʻemaʻe i hoʻohālikelikeʻia. ʻO ka papaʻaina ma lalo nei e hōʻike ana i kēia mauʻokoʻa:

ʻO Ficoll overlay Technique no PBMC e hoʻokaʻawale

ʻO keʻanoʻo FICOLL ma luna o keʻano he ala ākea i hoʻohana nuiʻia no ka hoʻokaʻawaleʻana i nā pbmcs. Hoʻopili kēia kaʻina hana i ke koko i luna o kahi ficolll - Paque solution solution a me ke kauʻana iā ia e hoʻokaʻawale i nā censity. Hoʻokumuʻo PBMcs i kahi papaʻokoʻa ma waena o plasma a me ka ficoll, e maʻalahi ana e hōʻiliʻili.

E hoʻoikaika i nā haʻawina i ke koʻikoʻi o ka hana ponoʻana i kēia kaʻina hana e hōʻoia i nā hopena kūlike. No ka mea, ua loaʻa kekahi haʻawina hoʻokahi e hoʻohana pono ana e hoʻohana pono i ka ficolll hiki ke hoʻokō i kahi maʻemaʻe o 97% me ka loli liʻiliʻi. Hoʻohālikelike ka papaʻaina ma lalo o nā hana incubationʻokoʻa no ka pbmc e hoʻokaʻawale:

Nā hana hoʻokaʻawale o IMHuamomagnetic

ʻO ka hoʻokaʻawaleʻana i ka hoʻokaʻawaleʻana i kahiʻoihana'ē aʻe no ka hoʻokaʻawaleʻana i nā pbmcs. Hoʻohana kēiaʻano hana i nā bead magnetic beated me nā antibodies e hoʻokūkū i nāʻano kelepona kūikawā. ʻO ka hoʻonohonohoʻana i nā celltates maikaʻi ma ka hoʻopaʻaʻana iā lākou i nā beads,ʻoiai e hoʻokaʻawale ana i nā cell i makemakeʻia.

Hōʻike ka noiʻi i nā mea maikaʻiʻole e mālama pono ai i ke kelepona aʻaʻole e pili i nā māka hana e like me ka mea hana e like me Il - 2r (CD25). Ma ka hoʻohālikelikeʻana, hiki i keʻano kūpono ke hōʻemi i ka hiki ke hōʻemi i ka hiki ke hōʻemi a me ka hana hoʻoikaikaʻana,ʻoi aku ma hope o ka hoʻouluʻana. ʻO ka papaʻaina ma lalo nei e hōʻuluʻulu i kēia mauʻike:

He mea maikaʻi loa kēiaʻano inā makemakeʻoe i nā helu kelepona nui no ka noiʻi a iʻole nā ​​noi a nā mea noiʻi.

Nā noi o nā PBMcs kanaka i ka noiʻi a me ka lāʻau lapaʻau

Role ma ke kaʻa - t cell therapy inrapy

E pāʻaniʻo PBMcs kanaka i kahi hana pivotal i ke kaʻa holomua. ʻO kēia mau mea lawelawe e lawelawe ai e like me ka mea hoʻomaka no ka hoʻouluʻana i nā kaʻa - T mau cell, e hana ana i nā mea hoʻokele a hoʻopau i nā cell cell cell. Manaʻo pahaʻoe i ka pehea o ka pbmcs kūpono i kēia kaʻina hana. Hōʻike nā'ōkuhi i nā hopena kupaianaha:

• Ma hope o 11 mau lā o ka moʻomeheu, 1 × 10 ^ 7 mau brozen pbmcs e hiki ke hana ma ka liʻiliʻi 1,28

• Hōʻike like nā hōʻikeʻo Cytotoxicicy me MesocCar -ʻO nā cralls i loaʻa mai nā mea hou a me nā cryopres pono e like me nā pbmcs. Ma kahi hopena - I - Ka hoʻonohonohoʻana o ka ratio o 4: 1, i kā lākou cytotoxicity ma waena o 91.02 - 10085.4%, e pili ana.

• ʻOiai ma kahi haʻahaʻa haʻahaʻa o 2: 1,ʻaʻoheʻokoʻa koʻikoʻi i ke cytotoxanity i nānāʻia.

Hōʻike kēia mauʻike i ka hilinaʻi o nā PBMcs i ka hanaʻana i nā kaʻa maikaʻi.

E hoʻohana i ka hoʻomaʻamaʻaʻana i ka lāʻau lapaʻau a me nā haʻawina haumāna

ʻO nā PBMcs i mea nui loa i ka hoʻomaʻamaʻaʻana i nā lāʻau lapaʻau a me nā haʻawina gicsity. Hāʻawi lākou i kahiʻano kanaka.ʻO ka hoʻohālike kūpono e loiloi ai i ke kauʻana o nā lāʻau lapaʻau e launa pū me nā cell invumune cell. ʻO ia hoʻi, ua ho'āʻo nā mea noiʻi i ka lāʻau noiʻi ma PBMcs ma PBMcs eʻike i kāna hoʻolauleʻa. ʻO ka papaʻaina ma lalo nei e hōʻuluʻulu i nāʻike:

Hōʻike hou kēia mau hopena i kēlā mau pbmcs i pane nui aʻe iā Tilinacrine, aia i nā pae haʻahaʻa. Hana kēia iā lākou i kahi hana hilinaʻi no ka wānanaʻana i nā pane hou i nā lāʻau hou.

ʻO ka biormarker i kaʻike a me ka nānāʻana

Pono nā PBMcs no kaʻikeʻana i nā bioglars a nānā i ke olakino olakino. ʻO nā mea hoʻohālikelike o nā biororkes e nā hōʻailona o nā kaʻina hana a iʻole nā ​​maʻi. Ma ka nānāʻana i nā PBMcs, hiki iāʻoe ke wehe i nā biogrars e hōʻike i nā hana hana, ka holomua o ka maʻi maʻi. No ka laula, ke helu pinepine nei nā mea noiʻi ma PBMcs i ka nānāʻana i nā pane i hala a iʻole nā ​​mea hana hewa. Kōkua kēia alapine i nā hana hana hana i nā mea maʻi i nā maʻi pilikino, nā hopena hoʻomaikaʻi.

Ua hiki i nā Pbmcs ke lōʻihi - Term Tommune nānā. ʻO kā lākou kūpaʻa a me ka hoʻololiʻana iā lākou i mea kūpono no ka nānāʻana i nā loli i ka hana i ka wā o ka manawa. He mea pono kēia i nā maʻi maʻi a iʻole i nā hana lōʻihi.

ʻO nā pbmcs kanaka he mea nuiʻole i ka hoʻomaopopoʻana a me ka hoʻomaʻamaʻaʻana i ka immunology. ʻO kā lākou huiʻana-lymphocytes, monocytes, nā monocytes, a me nā condritic cell-hiki iā lākou ke hana iā lākou e hana i nā hana koʻikoʻi. ʻO nā hana kālepaʻana e like me ka ficoll overlay and immunomagnetic e hōʻoia e hiki iāʻoe ke loaʻa ke kiʻekiʻe.

ʻO kā lākou mau noi i hoʻohālikelikeʻia i kahi ākea o nā mahina:

• Ua hoʻohanaʻia nā kaukani o nā haʻawina i nā pbmcs i nā noiʻi clinical ma luna o nā makahiki he 50 i hala.

• He mea nui lākou no ke kaʻa - T Cals Therappy

• Hāʻawiʻo PBMcs i ka loaʻaʻana o Biormarker Fiory,ʻo Stratifice e hoʻomaikaʻi, a me kaʻimi noiʻi maʻi maʻi.

Ma ka leveraging PBMcs, hiki iāʻoe ke wehe i nā mea hou ma Immunology a hoʻomohala i nā hana ola.

FAQ

He aha nā pbmcs i hoʻohanaʻia no ka noiʻi olakino?

Ua kōkua nā pbmcs i nā mea noiʻi noiʻi i nā mea kūʻai aku, e ho'āʻo i nā lāʻau lapaʻau hou, ae hoʻomohala i nā hana lapaʻau e like me ke kaʻa - ʻO kā lākou hoʻololi a me ke kūpaʻa e hana ai iā lākou i kūpono no nā hoʻokolohua e pono ai i nā cell gellune.

Peheaʻoe e kūʻai ai i nā PBMcs no ka hoʻohanaʻana i ka wā e hiki mai ana?

Hiki iāʻoe ke mālama i nā pbmcs ma ke kīʻana iā lākou i loko o ka nitrogen wai. Mālama kēiaʻano hana i ko lākouʻano a me ka hana no nā makahiki, eʻae iāʻoe e hoʻohana iā lākou i nā noi lōʻihi.

Nā pbmcs e like me ke koko keʻokeʻo keʻokeʻo?

ʻAʻole pololei. ʻO PBMcs kahi'āpana o nā koko koko keʻokeʻo e komo i nā lymphocytes, monocytes, a me nā cell dendritic cell. Ke haʻalele nei lākou i ka granuloucytes e like me nā neutrophils,ʻo ia kahi'āpana o nā kahe koko keʻokeʻo.

Hiki i nā pbmcs ke hoʻohana e aʻo i nā maʻi autoommune?

ʻAe! He waiwai nui nā PBMcs no ke aʻoʻana i nā maʻi autoommune. Kōkua lākou iāʻoe e kuhikuhi i ka hana cell cell, nā hana cytokine, a me nā hōʻailona genetic, e mālama ana i nā mea lapaʻau i loko o nā maʻi maʻi maʻi.

He pbmc e hoʻokaʻawale i kahi kaʻina paʻakikī?

ʻaʻole naʻe. ʻO nāʻenehana e like me keʻano o keʻano ficoll ma luna o ke ala a iʻole immumomagnetic e hoʻokaʻawale i ka pbmc i pololei. Me nā hoʻomaʻamaʻa kūpono a me nā lako hana, hiki iāʻoe ke loaʻa i nā kiʻekiʻe - hoʻomaʻemaʻe mau maka no kāu noiʻi.