Nhanganyaya kune B maseru uye kukosha kwavo
Muwebhu rakaoma rewebhu yemuviri wemuviri immune system, b masero anotamba chinzvimbo chakakosha. Semhando yerudzi rwechena eropa, b masero eB, B masero anowanzoitika kumhinduro yekushambadzira yekuchinja, inonyanya kukonzera kugadzirwa kwemanyodhi emitambo. Kugona kwavo kusarudzika kuziva maAntigens uye kusakwana kwavo kwakakosha mundangariro dzengozi kunovaita nyaya yakakosha yekudzidza immune yekudzivirira. Kunzwisisa maitiro ekuzvidza zvinobudirira b masero b
Kunzwisisa B Cell yekuzvitsaurira: Key concepts
B Cell yekuzviparadzanisa ndeye yakakosha mukutsvagurudza kwacho kunosanganisira kupatsanurana b masero kubva kune huwandu hwevanhu vakasanganiswa. Chinangwa ndechekuwana yakachena B cell yeBero kune dzakasiyana siyana zvikumbiro, kusanganisira inoshanda assays, Gumi Expression zvidzidzo, uye zvinoitika zvinoitika. Maitiro ekuzviparadzanisa anotenderera pane akasiyana maruva akataurwa paB maseru, ayo anovabvumira kusiyaniswa kubva kune mamwe marudzi echitokisi. Zvakaita se "B Cell Inosarudzika Kit"Inova yakakosha sevatongorori nzira dzisingaverengeki uye zvishandiso zvekubatsira kuedza kwavo
Kugadzirira samples for b cell yekuzviparadzanisa
Inoshanda b cell yekuzviparadzanisa inotanga nekugadziriswa kwekugadzirira kwemasampuli. Maitiro acho anotanga neunganidzwa weropa kana tishu samples, ayo anozoitwa kuti awane imwe - cell kumiswa. Kubata kwakakodzera uye kuchengetwa kwemasampuli kwakakosha kuchengetedza cell kubudirira uye kutendeka, izvo zvinokanganisa kubudirira kwesangano rekuzviparadzanisa. Kuona kuti samples inochengeterwa mamiriro akakwana asati azviparadzanisa nenjodzi yekusvibisa cell yekusagadzikana uye kusvibiswa, nekudaro achiwedzera kunaka kwemasero ega ega. Tsime
Nzira dzeB Cell Kuzvitsaurira
Nzira dzakasiyana siyana dzinovapo dzekuzviparadzanisa kweB, imwe neimwe nezviitwa zvayo uye kufunga kwavo. Pakati pehunyanzvi hunowanzo shandiswa dentity gradient centrifugation, magineti - activated cell kuronga (Macs), uye fluorescence - activated cell kuronga (fithi). Nzira imwe neimwe yakagadzirirwa kupatsanura B masero anobva pane chaiwo hunhu hwakadai sehukuru, density, kana kutaura kwevakaomesa maseti. Kune vanotsvaga kutsvaga kugona uye kururamisa, kusarudza nzira yakakodzera uye yakakodzera "B Cell yekuzvisarudzira kit" yakakosha. Kunzwisisa iyo nuances yehunyanzvi yega yega inobvumira vaongorori kuti vagadzirise kusvika kwavo maererano nezvakangoedza zvekuedza uye zvaunoda zvido.
Magnetic - Activated Cell Sorting (Macs) yeB masero
Macs maitiro anozivikanwa eB Cell yekuzviparadzanisa, achivhura musimboti wekuparadzaniswa kwemagineti kuti afukezve sero. Nenzira iyi, masero anoverengerwa nemagineti antibodies anotarisira B cell - yakatarwa mamaki. Kamwe akanyorwa, munda wemagineti unoiswa kuti uparadzanise iyo yakanyorwa masero akanyorwa kubva pakuzorora. Iyi nzira inobvumidzwa kuti ive nyore uye nekukurumidza kugadzirisa, ichiita sarudzo yakasarudzika yemarabhoritari mazhinji. Wholesale b cell yekuzviparadzanisa kits inowanzove neese zvinhu zvinodiwa macs, kupa yakaenzana uye mutengo - mhinduro inoshanda kune vanoongorora pasi rese.
Fluorescence - Yakagadziriswa Cell Sorting (FACS) maitiro
FACS ndiyo nzira yakanyanya kunaka iyo inobvumidza yakakwira - resolution b cell yekuzviparadzaniswa maererano nekutaura kweiyo fluorescently taggedies. Iyi nzira haingozviti chete b masero asi zvakare inopawo ruzivo rwakadzama nezve sero sero, kuoma, uye mamiriro ekuratidzira chiratidzo. Kugadziriswa uye kuchinjika kwedunhu kunoita chishandiso chine simba kune kuedza kwakanyanya uye kubereka kweB masero eB. Kune vakawanda vanotsvaga, kuisa mari mu b cell yekuzvipararira kit mugadziri iyo inopa FACTS
Zvinonetsa muB Cell Opolation
Pasinei nekufambira mberi mukuzviparadzanisa nevamwe nzira, vaongorori vanowanzo kusangana nematambudziko muB Cell yekuzviparadzanisa. Izvi zvinogona kusanganisira nyaya dzakadai seyakaderera cell kupora, kusvibiswa kubva kune mamwe marudzi emusero, uye technical kusawirirana. Kukurira idzi hurlsles dzinoda musanganiswa weProtocols Optimised uye yakakwira - mhando reagents. Kubatana neCLEBLE B CLELLECALINAT KIT SUPLILESS inovimbisa kuti zvigadzirwa zvinosangana nemhando dzemhando dzekutonga dzemhando, izvo zvakakosha kuti uderedze matambudziko mumarabhoritari. Uye zvakare, kugara uchiziva zviitiko zvitsva uye maitiro akanakisa anogona kubatsira mukudzora aya zviitiko zvekuzvisarudzira.
Kufunga nezve cell kusazviparadzanisa protocols
Kuti uwedzere kushanda zvakanaka uye kushanda kweB Cell yekuzviparadzanisa, zvakakosha kuti uwedzere protocol. Zvinhu zvakadai sekuvandudza mhando, nguva dzekupinda, uye matanho ekushambadzira anogona kukanganisa zvakanyanya uye kuchena. Kugadzirisa iyo yekuzviparadzanisa kwesoricol kuti igare chaiyo yekuedza mamiriro inogona kusimudzira zvabuda uye kutyaira kwakabudirira kutsvagira kuedza. Kushandisa bero rekusarongedza kit fekitori inopa sarudzo dzinopa dzesarudzo dzinobvumira vaongorori kuti vatorezve zviitiko zvavo, vachisimbisa kuti ivo vanoenderana nezvinodiwa zvakasiyana uye zvibodzwa zvesainzi.
Zvikumbiro zveSolated B masero mukutsvaga
Ari voga masero eB anoshanda semidziyo inowanzoitika mune dzakasiyana siyana dzekutsvaga maficha. Kushandisa kwavo kunowedzera kupfuura zvakadzikorodza zvidzidzo kune dzimwe nzvimbo dzakaoma seyakafanana nechirwere chekuenzanisira uye kurapa kwekusimudzira. Akasarudzika B masero anoshanda mukudzidza kweAutoimmune Disrid Uyezve, kufambira mberi mu cancer immunoththapy zvakanyanya kuvimba nekunzwisisa kudyidzana pakati pe b masero uye mabundu masero. Kuwanika kune yakachena b cell vagari vema cell, saka, inofambisa yakadzikama kutsvagisa iyo inogona kutungamira kuClinical gadzira uye nekuvandudza zvibodzwa zvemurwere.
Nzira yemberi muB Cella Recolate Techlation
Munda weB Spell Kuparadzanisa Kwese kuri kuramba uchimhanya, kutungamirirwa nekudiwa kwekushanda kwakasimba, kwakavimbika, uye kunotangira nzira. Kubuda kwehunyanzvi uye kune zvigadzirwa zvinovimbisa kusimudzira iyo precision uye chiyero cheB Cell yekuzviparadzanisa, zvinogona kubatanidza zvisingaitiki masisitimu uye epamberi bioinforms maturusi. Kubatana nePamberi - Kufunga B Cell Kuparadza Vatengesi veKutengesa uye Vagadziri vanoona kuti vaongorori vane mukana wekucheka - mhinduro dzekumedura dzinogona kushandura mawindo avo. Sezvo aya matekinoroji anokura, ivo vanobata mukana wekuita zvakanyanya kukanganisa zvese nekunyorera zvinyorwa, vachiridza nzira yekusawanikwa uye maratidziro ekurapa.
Mukupedzisa, kuparadzaniswa kweB kwemanyowa inhanho yakaoma mukutsvagurudza kwakawanda uye maciniki ekushandisa, kupa nzwisiso yehupamhi kunodiwa kuti ufambe kuchengetedzwa. Nekukwira kwakakwiriraIfase, vatsvagiri vanogona kukunda kusazviparadzanisa uye kuzadzisa zvinangwa zvavo zvesainzi.
About Pipase
Musoro wakadzika muNorth Wales, Pennsylvania, Pefese Boscience is "nyanzvi ye" nyanzvi, uye inokwirisa " Kuzvidza ruzivo rwakakura uye kusuwa kwesainzi Kutsvaga r & d yakanaka ye "Runyanzvi Regents
Post Nguva: 2024 - 10 - 15 10:59:52