Human Peripheral Ropa MononuClear Cell (PBMC)
PBMC inogona kutevedzera iyo - Vivo nharaunda yekupa inoshanda mu - vitro modhi yekukura kwezvinodhaka. Kufunda zvinokonzerwa nemishonga pane zvinangwa uye nenzira dzekuratidzira dzePBMC dzinogona kubatsira mukutsvaga zvinangwa zvezvinodhaka, uye idzi dzakawanikwa dzinogona kupa pfungwa dzisingagumi uye mafambiro ekuwana zvinodhaka uye kukura kwezvinodhaka uye kukura.
Iyo huru yekushandisa mafambiro ePBMC mune Immuniothapeutic Dulopment Budiriro inosanganisira:
1.nditi NK masero, Macrophages, etc. ndiyo huru inogadzirisa PBMCs mu Adcc chiito. Aya masero anogona kuuraya masero aanangwa akatarisa neantibodies kuburikidza nekusunga kwavari.
2.mixed lymphocyte reaction Dc cell vanoratidza huwandu hwakakura hwePD - L1 mune yavo pamusoro, iyo inogona kusunga uye inhibit pd - 1 pamusoro pe t masero. Kana mushonga wacho uchigona kukanganisa nzira yekuita pakati pezviviri, ipapo inogona kumutsa kuwanda kwe t masero uye nokudaro ichisimbisa basa remushonga.
3. Scer activation assays: T cell activation assays ndeimwe yemunhu anowanzo shandiswa assars yekuongorora mhedzisiro yemishonga yekuzvara. MuT cell activation assays, mu - Vitro tsika t masero anowanzo shandiswa uye akakodzera kukurudzira, akadai sekutadza kana antiodies kana antibodies, anopiwa kuti anyatsoita masero eT masero eT masero eT masero eT masero eT.
4. Cell Proliferation Assay: T Cell Proliferation Inogona Kushandiswa Kuongorora Impuntherapy Vanodhonza Vanodhonza uye Kuenzanisa Dhigiri reT Cell proliferation mune dzakasiyana mishonga -
Hunhu hwevanhu inogadzirwa naPHASE inoparara kubva kuRopa Revanhu Rinopararira neMeensity Gradient Centrifugation. Aya ndiwo masero mumwe chete nauclus anonyanya kuumbwa ne lymphocyte (t masero, b masero, uye masero eNK) uye monocytte.
▞ Ruzivo rwechigadzirwa:
|
Zita |
Item Nha. |
Kutaurwa |
Sero Mamiriro |
Kuchengetedza / Kutakura |
|
Human Peripheral Ropa mononuclear maseru |
082A01.11 |
Mamirioni mashanu masero / ml |
Nyowani |
2 - 8˚C yekuchengetedza, ice pack kutumira |
▞ Chigadzirwa Chigadzirwa:
In - vitro metabolism kudzidza kwezvinodhaka.
