511 - Raderctase Chiitiko Chekudzidza: SRD5A1 & SRD5A2 yeSybum Control muCosmetic Science

Mazwi akakosha: Type 1 5α - Ridterctase, Type 2 5α - Destctase,5 ,, 5αr2, sr5a1, srd5a2,NADPH, 511 - Ridctase Basa, 51K - Ridctase Inhibition, Dihydrotesterone (Dht).

Zvigadzirwa zvePhase

Induction

511 - Kuderedza iMicrosomal Enzyme Inotarisira Shanduko Testosterone mukatiDihydrotestostone (dht), zvakanyanya ane simba jegen. Kuita uku ndiNADPH - Kuvimba, kunoda nicotinamide deninedinucleotide phosphate (NADPH)kunge coffter. Dht Bigss kune Andergen Rewistors neTestosterone uye anoshanda mukudzora mabasa akasiyana siyana emuviri, kusanganisira kukura kwebvudzi, kukura kweProsteti, uye sebhavha regland.

Isoenymes: Type 1 5α - Ridformeta & Type 2 5α - Ridctase

Kune maviri maOnzymes ye5α - kuderedza:

• -Type 1 5α - Kuderedza (SRD5A1): Kunyanya inoratidzwa muchiropa, ganda (kunyanya sebaceous glands), uye ganda. Iyi isoolordhi inoita basa rakakosha mune yeganda jegset ethabolism uye yakasungirirwa zvakanyatso kusungirirwa kuCobum Production.
• -Type 2 5α - Kuderedza (SRD5A2): Inowanikwa kunyanya muProstate, seminal vesicles, uye genital ganda. Kunyangwe basa rayo richinyanya kubatanidzwa nemitezo yekubereka, inopawo kumamiriro eganda munzvimbo dzinodzora.

Basa reDht muSamabum Proction

Dht Binda kune Androgen Receptors muSebaceous glands, inomutsa sebocyte prolifeation uye sebum securntion. Kuwedzeredza kweSumabum CLOG Pores, kugadzira nharaunda yakakosha kune Cutibacterium aces kukura, kuzvimba, uye acne. 511 - Kuderedza Type 1 (5DHADE), kuva ivo vanozvinyora paganda, saka ndiyo kiyi yekutarisa yekubatanidza cosmetic yakanangana nekudzora sebum - nyaya dzakabatana.

Kuongorora 5α - Kuderedza Inhibition: Chiitiko Assays

Kukudziridza uye kusimbisa cosmetic zvimisikidzwa izvo inhibita Maviri ezvimwe zvinhu zvakajairika imbudzi esentrophotometry uye mvura chromatography - Misa spectrometry (lc - ms).

Spectrophotsometric assay

Iyi nzira inotarisa iyo oxidation yeNaDPH, inoenda kuderedzwa kweTestosterone kuDht na5α - Ridctase. Sezvo Nadph inopedzwa panguva yeiyo enzyematic maitiro, hunhu hwayo hwekutora na 340 nm inoderedza, kubvumira chaiyo - nguva kinetic zviyero zve51K - Kuruma Basa.

• - Zvakanakira: Kurumidza, mutengo - Inoshanda, uye yakakodzera kukwirira - Kuburitsa kuongorora.
• - Zvisingakwanisi: Hazvina kukodzera zvakananga Dhit, saka zvinogona kufurirwa nemaitiro emapuranga kana kusvibiswa mukubvisa madhiri.

LC - MS assay

LC - MS (Liquid chromatography - Misa SpectRometry) inopa chiyero chakananga uye chakanyanya kuyerwa kweDht kugadzirwa kubva kuTestosterone. Iyo yekushandisa inoshanda inotanga kupatsanurwa kuburikidza ne chromatography, ndokuzoonekwa uye kuiswa uchishandisa mashandiro emisa.

• - MukanaS: Kunyanya kunzwa uye kujeka, kuyera kwakananga kweese substrate (testosterone) uye chigadzirwa (dht), ichiita kuti ive yakanaka yekusimbisa51K - kudzikisira kudzivisa.
• - Zvisingakwanisi: Zvakaoma kunzwisisa uye zvinodhura; inoda michina yakakosha uye hunyanzvi hunyanzvi.

Iyi nzira leffs ndeye zvisingaiti zvishandiso zviri muKuratidziro uye kuvandudzwa kweCosmetic mafomu ayo anovavarira kudzora kugadzirwa kwesbum uye inhibiting srd5A2 zviitiko.

Cosmetic indasitiri application: Sambum Control kuburikidza Inhibition

Iyo indasitiri yeCosmetic yakambundirana 5α - yekudzora inhibition sechirongwa chekurwisa chidimbu cheganda, acne, uye zvinoenderana nedunhu reDermatological nyaya. Nekugadzira zvigadzirwa nezvinhu zvinoderedza 5α - yekudzora chiitiko, mabhurawuti anongotsigira maSebum levels uye akavandudza kujekesa kweganda.

Commetic cosmetic zvisikwa ne5α - yekudzora inhibitory zvivakwa zvinosanganisira:

• - Zinc pca
• - Green Tea Extract (Epigallocatechin gallate)
• - Saona palmetto bvisa
• - Humpkin mbeu OIL
• - Aazelac acid

Aya mabasa anoitwa nekunyadzisa iyo srd5a1 (5α1) uye / kana SRD5A2 (5D 5) isoforms, nekudaro kuderedza iyo ganda dht mazinga uye nokudaro kuderedza kuderedza sebum kubuda. Izvi zvinokanganisa kuti nheyo dza5α - yekudzora inhibition yakabudirira zvakabudirira kubva musainzi yezvekurapa mumitambo yezuva nezuva.

Mhedziso

Iyo enzyme 5α - yekudzora, kunyanya kuburikidza neyakagadziriswa 1: Srd5a1) Ovesolom, anotamba chinzvimbo cheSumabum Proction kuburikidza neSynthesis yeDht. Iyo cosmetic indasitiri ye5α Sezvo kutsvagurudzo kunoramba kuchisvika, iyo enzyme inosara pamberi peimba yeDermocosmetic