CGT Innovative Therapy - Lentivirus - Packating Car - T Impuine Masero

CGT Innovative Therapy - Lentivirus - Packating Car - T Impuine Masero

Impy sero kurapwa kurapa kwegomarara kurapa kunowedzera immune yemuviri yekudzivirira kune chinangwa uye kubvisa cancer cancer cancer. Mafomu akakosha anosanganisira Cytokine - Yakamisikidzwa Killer Cell Therapy, T - Cell Therapy, uye Cars - T Cell Therapy. Car -

Keywords: Car - T, Lentivaram, Lentivaram Vector, Tracer Lentivirus

Kugadzirira Kwemotokari - T Cell kurapa

Kazhinji, matanho akakosha okugadzirira mota - Masero t seinotevera:

Nhamba yeMifananidzo: DANA - Farber Cancer Institute - Mota T - Cell Cliniki Miedzo Inobatsira Varwere Vemakore MyEloma vanodzokera kuhutano

1. 1.Sorting (T - Cell Kuzvitsaura)

Nhanho yekutanga mukugadzirira mota - T masero ndeyekuparadzanisa T - masero kubva pamasero machena eropa muropa remurwere. Nhungamiro dzakadai seNdmunomagnic Bead kupatsanurana kana kuyerera Cytometry vanowanzo shandiswa kuburitsa CD3 + - Rupfu T - masero kubva muropa. Kuve nechokwadi chekuchena kwesero, maitiro ekurongedza anofanirwa kuve akajeka kwazvo, kuve nechokwadi chekuti nhamba yakakwana yekukwira - Hunhu T - Masero anowanikwa.

2. 2.Stimulation (T - cell activation)

Iyo inobviswa t - masero akaiswa mu vitro, kazhinji achishandisa aCD3 / CD28 T - masero activation / expranssion kit. Activated t masero ent Inoshanda State, achigadzirira ivo kuchinjika kwenzvimbo. Kuburikidza nemaitiro aya, tero sero inosimbiswa, inopa kune inotevera genetic yekugadziridzwa uye kukwidziridzwa.

3. 3.transkes (gene kugadzirisa)

Activated t masero anoisa gene kuchinjirwa kuratidza chimeric antigence receptor (mota). Maitiro aya kazhinji anoshandisaLentivaram vectorkuunza majini emotokari mu t masero. Lentivirus chinhu chinoshanda chejenti yekufambisa, nemabhuku ekubatanidza zvine hunyanzvi Gene yakatarisana (senge car gene genome genome. Nekutapukira t masero uye kuchinjisa iyo mota gene gene in t cella, lentivirus inoita kuti masero eT all kuratidza mota refu - term. The CAR gene includes a single-chain variable fragment (scFv) of an antibody that enables T cells to recognize specific antigens on the surface of tumor cells and initiate an immune response by activating signaling regions of T cells. Sezvinoratidzwa mumufananidzo 2. Lenthivalral kutengeserana ine maitiro ekunyanyisa kugona uye kugadzikana, kuitira kuti genera yemota inogona kuratidzwa mu t masero kwenguva yakareba.

Mufananidzo Tsime: Singapore Car - T Cell Therapy

4. 4.Mamplization (cell Proliferation)

Mushure mekutanda, mota - t masero anopinda mukati mekusvika nhanho, pane zvinhu zvekukura sengeIL - 2inowedzerwa kusimudzira cell kuwedzera. Chinangwa chechiitiko ichi ndechekuti uve nechokwadi chekuti nhamba yakakwana yemotokari - ma cell inowanikwa kuti vawane pakupedzisira infusion mumurwere. Maitiro ekumabvazuva anofanirwa kuitiswa pasi pemamiriro ezvinhu akasarudzika kuti vazivise hunhu uye huwandu.

5. 5.Qullity Kudzora (QC)
6. Usati adzoreredzwa mumurwere, kutonga kwemhando yakakosha panguva yekugadzirira maitiro. Yakagadzirirwa Car COLD - T Masero Anodzvanya Kuedza kuIta shuwa kuchena kwavo, chiitiko, kushanduka-tsika uye kutevedzera zviyero zvekurapa. Pamusoro pezvo, kuyedzwa kwekusvibiswa kwesero kana kuti zvakashata zviitiko zvinodikanwa kuti uve nechokwadi chekuchengetedza uye ekurapa kushanda kwemasero mushure mekusadzoserwa mumurwere.

IMportance yeLentivirus inotsvedza mumasero emidziyo

LEntivirish transkection tekinoroji yakakosha mumusero wekudzivirira musero, kunyanya kune gene kugadzirisa uye cell engineering. Lentivaram vechikadzi, anotorwa kubva kuHIV Vanogona kutapura kupatsanura uye vasiri - masero ekukamuranisa, uye kuchengetedzwa kwavo kwakavandudzwa nekugadzirisa majini makuru ekudzivirira kudzokorora. Idzi midziyo ikozvino yave kushandiswa kuGene kurapa, gene kugadzirisa, uye cell biology kutsvagisa kwavo kugona kwavo uye kusimba kwezana muGene kuendesa.

Nhamba yeruzivo: Obiomh.com/products/ schematic dhizaini ye Lentivirus pacoring uye lentivirus transkection process mumasero mazana matatu nemakumi matatu nematanhatu

1.Effiffien genqikection

Lentivaram Vector anokwanisa kuburitsa zvimiro zvemajini, senge mota yemota, mumasero emagetsi okushayiwa simba. Iyi yakakwira - kushanda zvakanaka kushanduka kugona kwakakosha kubudirira kwebudiriro yekudzivirira.

2.lower immunogenicity 

Kuenzaniswa nedzimwe viratilers vezvivakwa, Lentiviruse ane yakadzika immunogenicity. Nekuda kweizvozvo, apo lentiviruse ishandiswe kushanduka masero erivine, immune system yemurwere inogadzira kusasimba kuneta kune hutachiona hwehutachiona. Izvi zvinobatsira kuderedza kushanda kwakashata, kunyanya munguva refu - nguva yekuponeswa kwemasero mushure mekunyengera, nekudaro achidzivirira kudzivirirwa kwakanyanya.

3.high accaptability kumasero emhando yemasero

Lentivaram vector vanokwanisa kushandura zvakanyanya kushandura kwakasiyana siyana mhando dzemasero emuviri mese,kunyanya t masero uye nk masero. Kuburikidza neLentivaram Campuction, masero emuviri anogona kuwana bundu - kugona kugona kwekuziva, nekudaro achiwedzera kugona kwavo kuuraya uye kuuraya mabundu.

4.Procicision yemasero ekutambuka anogadzirisa mukucherechedzwa cell cell 

Mukudzivirira,Shanduko Yakasarudzika MaseroTarisa uone masero emuchina eillini kuti, mushure mekushandurwa, kuratidza mota uye kushandisa anti - bundu basa. Lentivaram Vectors anogona kuendesa iyo Gene Gene mumaseru aya anodzidzisa, achivigira kuti vazive uye vanonzwira mabundu.Target maserondiwo masendimita ebundu anozivikanwa uye akaparadzwa nemisoro iyi immune masero. Kuburikidza nekududzirwa kweLentivaram, masero emuviri anogona kusimudzira chokwadi chavo mukuziva uye kuuraya mabundu embato, nekuvandudza kushanda kwekuratidzira.

Mundima iyi, iyo Phaase, mutungamiri muVitro Researching Regents Izvi zvigadzirwa zvinopa imwe - STOP Solution kune vatengi.

Ifase LentivirusProducts

Iyo Iphaase Lentivirus Pacing kitine zvese zvakakosha reagents anodiwalentivirus kurongedza, kurerutsa zvakanyanya maitiro ekuedza uye nguva yekuponesa uye kushanda nesimba. Iyi kit inosanganisira:

• Lentivirus Packaging Plasmid musanganiswa
• Chinja Reagent
• Egfp Plasmid
• Ifase Lentivirus Constration Reagent

Iyi kit iyi inotenderera zvechokwadi muyedzo! Pasina chikonzero chekugadzirira kugadzirira, kurongedza kweLentivirus kunogona kupedzwa zvakanaka kuburikidza nekutevera mirayiridzo yaisanganisira.Izvo zvakanakira kitidzo dzakataurwa kitamba pfupi dzekutakura, dzakakwirira dzimba dzegumi, uye nyore kushanda, zvichiita kuti zvive zvakanaka kuti vatange kurongedza muLentivirus kurongedza. Seti imwe neimwe yedzinza iri pasi pehurongwa hwakaomesesa kuti uve nechokwadi, uye zvakavimbika, uye zvinovandudza zvakanyanya zvekushandisa uye nekugadzikana kwehutachiona hwehunyanzvi hwekugadzirira kwemasero.

Pamusoro pezvokubatsira vatengi kuchengetedza mari, Iphase yakagadziridza zvakare uye yakagadzira iyo Lentivirus yekuvandudza, ichipa mhinduro yekukurumidza uye yakapusa.Nekungobatanidze Lentivirus Supernant neCustomerant Regent Maitiro aya haade kuti ultracantrifuge, ichiita kuti ive nyore uye mutengo - sarudzo inoshanda kune vashandisi vazhinji vemarabharati.

Muchidimbu, iyo Iphase Lentivirus Regentation haina kungogadzirisa zvakanyanya kushandira kwekunyora asi zvakare inopa yakakwira - mhando dzeViral vector rutsigiro rwekugadziriswa kwemuviri. Icho chigadzirwa chakakosha chekuedza kwemaitiro, preccinical kutsvagisa, uye dzimwe nzvimbo dzekushandisa.

Mukupfupikisa, immamuritherapy, kunyanya mota - T Cell uye mota - NK Cell maraphies, inomiririra nhungamiro itsva mukurapa kwemazuva ano. Kuburikidza nemaitiro echokwadi senge sero kuronga, kushandurwa, kushandurwa, kuwedzera, masayendisiti anogona kugadzirira mari yakasarudzika uye yakachengeteka mmummune yevarwere, kugonesa kurapwa kenza.Shanduko yeLentivirish, sehunyanzvi hwepfungwa, rinotamba chinzvimbo chakakosha mukugadzirira mota - T uye mota - NC maseru nekuda kwekubudirira kwayo uye kugadzikana.Nezve kufambira mberi kwehunyanzvi, immunommyrapy inobata mukana wekupa sarudzo dzekutarisa kune vamwe varwere, kupa yakakura kiriniki kukosha. Mune ramangwana, immunotherrapy inotarisirwa kuunza shanduko yekudzivirira yekurapa kune vamwe varwere vechanza.

Kuti usangane nezvinodiwa zvejenje Aya tracer lentiviruse akarongedzwa neGreen Fluorescent Protein (GFP), Red Fluorescent Prretin Matchy, uye Luciererase, Kugonesa Kutsvaga Kurerutsa Cell zvese muVitro uye muVivo.

Kunze kwezvo, Iphase inogona zvakare kupa maviri pre - Aya maturusi anogona kusarudzwa kana kusanganiswa zvichida pane zvekuyedza zvinodikanwa, kuisidzira mabasa uye nekuwedzera kushanda kwechokwadi - nguva yekutevera uye kuongorora uye kuongorora kwemasero anangwa.

IfaseImmunotsrapyRakazaraProducts

Pamusoro peLentivirus - Zvigadzirwa zvine chekuita nemotokari - T kana mota - Iphase yakagadzira uye inogadzira leukocytes uye PBMCS (Peripheral Ropa mononuclear masero) akasarudzika kuburikidza neLukapheresis, pamwe chete neisimple immune cell yelation kits, kutsigira kukurumidza kukura kwe whounotherapy.

Zvigadzirwa zvePhase zvinopa zvibodzwa zvinotevera, uyeChitupa chekuongorora (Coa)Verified nePHASE inowanikwa kune imwe neimwe batch.

Kuchengetedzwa 

Zvigadzirwa zvePhase zvinobva kune vane hutano vanobata / mhuka dzine undergone through pre - kuongorora, nemhedzisiro yakaipa yekuedza (HIV - 1/2, HBV, HVC, Syphilis).

Kutevedzera  

Phepase inopa mafomu ehurukuro abvumidzwa nevanopa, aine akajeka uye anotaridzika uye anoteedzera Source sosi.

Hunyanzvi

Zvigadzirwa zvakapihwa naPiphaase, wakawanikwa kuburikidza neLukapheresis, anobatwa nevanyengeri vakadzidziswa. Batch yega yega inoperekedzwa neCoa inoenderana, uye zvigadzirwa zvinotakurwa pasi pechirongo chakaomarara kuti chengetedzwe, kukurumidza kuburitsa, uye kusimbisa kwemhando yepamusoro.

Mabatiro evatengi

Mufananidzo unopa wakakwira - Hunhu mushure - Kutengesa basa, kuchiva chiitiko chakatsiga uye chisina kuvhiringidzwa mumuedzo mumamiriro ezvinhu mumuedzo.