Cross marudzi cerbropinal fluid ongororo (CSF ongororo) uye yekuongorora matrix

1 zvigadzirwa zvePhepase

2 midiki mabasa e cerebropinal fluid: Bioanalysis yehuropi madhiragi

Uropi hwakakamurwa kuita zvikamu zvina, kusanganisira iyo telancephoron, Diencephon, cerebellum, uye brainstem. Matsara munzvimbo dzakasiyana siyana ehuropi anonzi ventricles, ayo anozadzwa necerebropinal fluid (CSF). Cerebropinal fluid inonyanya kubatanidzwa mukuparadzira uye metabolism yemishonga muuropi uye yepakati system system (CNS).

Pharmacodynamocs anodzidza shanduko yesimba rekusimudzira kwezvinodhaka Ropa - Brain Barrier (BBB) ​​isimba rechinguvana Kana bbb ichichinja mishonga yekuvhiringidza, kuona kugadzirwa kweropa zvinodhaka kunogona kunge kusiri kunyatsoratidza mushonga wekusangana muuropi. Kungoita kushandisa ropa zvinokonzeresa zvinodhaka zvechiratidzo chekusangana kwechiitiko chehutano hwepakati Naizvozvo, paMacro Level, uropi tishu homogenizization kana cerebropinal fluid nzira dzekurima inonyanya kushandiswa kune zvidzidzo zvePharmacokinetic muuropi.

3 kukosha kweCerebrobropinal Fluid kuongororwa mukusimudzira zvinodhaka

Iyo protein yemukati yeCSF yakanyanyisa kuderera, uye kushushikana kwayo zvinodhaka kunowanzo shandiswa sechaitsvaga chiratidzo chemishonga yemahara. Nekudaro, kushanda kweCSF kutenderera kwakanyanya kupfuura iko kwekutenderera kweropa, zvichikonzera kusasanganisa zvisina kukwana kwezvinhu zvemhuka zveCSF. Zvichienderana neiyo sampling nzvimbo / nguva uye nzira yekutonga, iyo yekuvhiringidza zvinodhaka muCSF inogona kusiyana zvakanyanya.Kugadzira Cerebropinal Fluid (Kugadzira CSF, ACSF) ndiyo mhinduro inotevedzera kuumbwa uye kushanda kweyakasikwa cerebropinal fluid, uye inogona kunyatsoongorora CNS pharmacokinetic data muVitro Adme AdmE FERIVE. Mukuwedzera, kugadzirira cerebropinal fluid kunogona zvakare kushandiswa kuongorora iyo mhedzisiro kwayo paBloun Edema uye Rondedzero chaiyo.

4 Zvinetso zveCerebrobropinal Fluid ongororo uye zvinodikanwa zveAngaimpinic Blank Matrix

4.1 yetsika / kiriniki zvisingakwanisi kuwana zvakasikwa cerebropinal fluid

Kuwana kweyakasikwa cerebrobropinal fluid ichiri kutarisana neThe Mattical Bottlecks. Vanhu vane hutano vanofanirwa kuwana cerebropinal fluid kuburikidza neLumbar puncture kana ventriculy drainage, iyo inoisa njodzi dzematanho ekusanzwisisika uye zvinoita kuti zviome kunyorera vane hutano vanozvipira zvine hutano. Uchishandisa officice cerebropinal fluid /simerlated cerebropinal fluid(Artificial CSF /simured csf)) Matrix panzvimbo yemasampuli echisikigo emitambo, achidzivisa nyaya dzetsika, kuona kuongororwa kwakagadziriswa apo kuchengetedzwa matrix maitiro (akadai sepuroteni yakaderera uye madziro eElectrolyte).

4.2 Matrix mhedzisiro yakakonzerwa nemisiyano yakakosha yemunhu

Misoro / misiyano yemunhu wese muProtein Zvemukati (15 - 100 MG / DL) Nekugadzirisa zvimisikidzwa zvimisikidzwa. Izvo zvakare zvinokwanisika kugadzirisa iyo nhare chikamu (senge hilic chromatography) kana kuwedzera nguva yekuchengetwa kuti uzviparadzanise zvinhu zvevanotarisana nazvo kubva kumatresfering zvikamu.

4.3 Iko kukanganiswa kwekuchinja mune zvakasikwa matrix zvikamu pakuongororwa pasi pemamiriro ezvinhu echirwere

Chirwere Cerebropinal Fluid inogona kunge iine mapuroteni asina kujairika (eropa -

5 Cross mhando cerebropinal fluid

5.1 human cerebropinal fluid(Human CSF)

Human cerebropinal fluidndeye cerebropinal fluid yegoridhe yakajairika matrix matrix, asi miganho yetsika inotungamira mukushomeka kwemasirimu echisikigo; Low Protein (15 - 45 MG / DL), Low Celly Zvemukati, inoda yakakwira - Sensitivity LC -

5.2 cynomolgus monkey cerbropinal fluid (Monkey CSF / NHP CSF)

Cynomolgus monkey cerbropinal fluidndiye asiri - Yevari yemhando yekutarisira iri padyo nevanhu, nemisiyano yezvidiki yemunhu, yakakodzera pk / pd uye yekuongorora yekuchengetedza yeCommany Cns Zvinodhaka, uye ndiwo muenzaniso wakasarudzika wakatarwa wezvidzidzo zvepanyama zvezvakawanda zvemishonga zvinodhaka.

5.3 Rhesh Monkey Cerebropinal Fluid (Monkey CSF / NHP CSF)

Rherus monkey cerbropinal fluidInofananidzwa neCynomolgus monkey, ine simba neuroimmune mhinduro uye yakakodzera kune neuroinfirating nehurwere hwakaringana nehurongwa hwekuzvishingisa kana zvinodhaka zvekumanikidza.

5.4 Beagle imbwa cerebropinal fluid (Beagle imbwa csf)

Beagle Ceibropinal FluidIcho chakakwirira protein tolerance modhi, iyo yakakodzera kuongorora ropa

5.5 SD rat cerebropinal fluid (Rat csf)

SD rat cerebropinal fluidndezvechidiki vhoriyamu cerebropinal fluid, nekushongedza yakakwira - Kuchinjisa kuongorora, mutengo wakaderera uye nyore kushandiswa kwechimiro, icho chinonyanya kushandiswa mukudzidza kweiyo neurodegegenerative chirwere chechirwere chechirwere.

5.6 Mouse Cerebropinal Fluid (Mouse CSF)

Mouse Cerebropinal FluidMuenzaniso wakakosha wekuyedza muloscience tsvakiridzo, kunyanya kushandiswa muGeMe yakagadziridzwa zvirwere (zvakadai sehosha yaAlzheimer, chirwere cheParkinson) uye Central system (CNS) kuongororwa kwezvinodhaka.

5.7 Minipig cerebropinal fluid (Minipig csf)

Minipig cerebropinal fluid 's anatomical chimiro chinobvumira kudzokororwa sampling, ichiita kuti ive yakakodzera kwenguva yakareba - Nekudaro, kuumbwa kwe phospholiprid zvakanyanya kubva kuvanhu, uye iko kusarudzwa kwenzira inoda kusimbiswa.

5.8 Rabbit Cerebropinal Fluid (Rabit CSF)

Rabit CSF Imwe yeiyo inowanzo shandiswa miedzo yekuedza muNouroscience Research

Mhedziso

Kuongorora kweCeerebropinal Fluid (CSF) inotamba chiitiko chechiitiko chepakati (CNs) zvinodhaka muropa Nekudaro, kushandiswa kwechisikigo CSF ​​kwakatarisana nematambudziko akakosha, kusanganisira zvimiro zvekuunganidza mumuenzaniso, inter - marudzi uye intersocullocation muMatrix kuumbwa, uye yakashandura biochemical mechi mune chirwere. Izvi zvinogumira pasi zvinokosha zveAcsf matrices, ayo anogonesa akaenzana uye kubereka bioanalytical bioanalytical assays paunenge uchideredza tsika uye technical zvipingaidzo.

LC - MS / MS yakabuda seyakakosha yekuongorora chishandiso cheCSF zvidzidzo, kupa yakanyanya kunzwa uye chokwadi kune zvinodhaka zvinodakadza uye biomerker kune yakaderera kutarisisa. Zvakangodaro, Matrix Mhedzisiro-inotungamirirwa nemusiyano muProtein, LIPID, uye electrolyte zvemhando dzakasiyana-siyana marudzi-inoda kunyatsogadzirira kugadzirira uye chromatographic mamiriro. Kugadzira maCSF matrices, akagadziridzwa kune mmmic ese ane hutano uye egarilogical mamiriro (e.efsi, akakwiridzirwa,

Pakati pemhando yemhuka, maCynomolgus monkey akafanana zvakanyanya kufanana nevanhu CSF uye anosarudzwa yeCSF Rollent Models (i.e., SD makonzo) Kupa mutengo - Inoshanda

Mukupedzisa, kuunganidzwa kwemarudzi - Chairo Chekutanga CSF matrices ane Adrics LC - MS / ms Nzira Dzinodzora Bhodhi muCNS Dishment, Kugonesa Zvakanyanya, Tsika Dzakanaka, uye Rinobudirira Pfungwa Dzidziso. Kufambira mberi kwemberi kunofanirwa kutarisa pakunatsa pathological csf mhando dzeCSF uye kururamisa bioanalytical vabatike kuti zvifadze kiriniki.

Mazwi Akakosha: Cerebropinal Fluid, Cerebropinal Fluid Fluid, Simpines CSF, CSF sampuli, CSF), Rhersus Monkey Cerebropinal Fluid (CSF), Monkey Cerebropinal Fluid, NHP CSF, Beuger CSF CSF, Beagle Imbwa CSF, SD Rat Csf Cerebropinal fluid kuongororwa

Cite: Ban Wei - Kang, Yang Zhi - Hong. Maitiro ekutsvagira uye epamberi tekinoroji anofambira mberi muIntraceremberal firmacokinetics. Chinese Chinese Papmacological Bulletin, 2023, 39 (9): 1607 - 1612.