Sploncytes mukutsvagisa zvivakwa: kuyambuka marudzi uye kuzviparadzanisa, kusunungura, thawing matanho

Mazwi akakosha:Sploncytes (Splans); Cynomolgus monkey splonowtes; Rheshsus monkey splonowcyte; Imbwa splonaytes; Canine splonipter; Rat splonaytes; Mbeva splonipyte; Makonzo Splonipincytes; Rabbit Splonomputte; Splocomputte kuzviparadzanisa; Kusunungura splonaytes; Thawing splonowcytes

Chigadzirwa chefae

Sploncytes (SPLS)Hutsinye hune hutsinye hwemasero emuviri akasarudzika akasarudzika kubva mukubwinya, humbowo hwakakosha hunobatanidzwa muropa rekusvibisa, rinokwira mhinduro dzvuku, uye kuchengetedza imbaostasis. Aya masero anosanganisira lymphocytte (t masero, b masero bS), Macrophaes, masero emazwi, uye masero eNC), ese anotamba mabasa okushanduka uye asina kudzivirirwa. Sploncytes anoshandiswa zvakanyanya mukuratidzira uye kushandura kutsvagurudza kudzidza immune basa, vaccine kukura, zvirwere zvezvirwere zveuto, uye cancer immunotherapy.

Basa uye Rugwaro rweSploncytes (SPLS)

Mukati meiyo Spleen, Splimocytes inogara munzvimbo dzakasiyana-siyana: Iyo tsvuku yeropa, iyo firita yeropa uye kudzokorora matsvuku matsvuku eropa masero, uye pulp chena, uko mhinduro dzisina njodzi dzakatangwa. Iyo chena pulp yakarongedzwa muT Cell zones uye B cell follicles, kufambisa kutaurirana pakati peantigen - Kuburitsa masero uye lymphocyte. Ichi chivakwa chakasarudzika chinoita kuti Splonowcytes (splums) chishandiso chine simba mune fungidziro fungidziro yakadai seElispot, muchengeti wekuyerera, uye wakasanganiswa Lymphocyte maitiro.

Mhando - Chairo Sploncytes (SPLS)

-Cynomolgus Monkey Splonows:Cynomolgus Monkey Splonowtes anoshandiswa mukutsvaga preclini

-Rherus Monkey Splonowcytes:Rhtserus Monkey Splonowtte anokosheswa nenzira yakafanana kune yavo yekusagadzikana madhiri kufanana nevanhu. Zvidzidzo uchishandisa Rherus Monkey Splonowcytes inobatsira bhiriji pakati pemhando dzemhuka uye clinical application.

-Imbwa Sploncytes / Canine Splonaytes:MuVeterinary kutsvagurudza, imbwa splonowcytes (kana canine splonowcytes) inoshandiswa kudzidza immune kusagadzikana mumarinari uye kuenzanisa kuenzanisa nemhinduro dzevanhu.

-Rabbit Splonowcytes: Rabbit Splonowcytte yakakosha muzvidzidzo pamusoro peanti Production uye yekudzima vesimendi. Rabbit Splocytes inobatsira kuburitsa ruzivo rwunogona kusiyana neye mbeva splonowtte kana rat splonaytes.

-Mbeva sploncytes / makonzo splonowtte:Mbeva splonocytte ndeimwe yeanowanzodzidziswa sploncytes (splusi) mukuisa kuchengetedzwa. Maprotocols yekuzviparadzanisa kubva pamakonzo akasimbiswa kwazvo, uye ese mbeva splonowincytes uye mbeva splonowtes anoshandiswa kuchinjisa cytometry, Elispot, uye zvimwe zvinhu zvinoshanda.

-Rat splonaytes:Rat Splonowincytes inopa muenzaniso unowirirana kuti immuntoxicology ne yekudzivirira zvidzidzo. Rat splonowste anowanzoenzaniswa ne mbeva splonows kuti vanzwisise misiyano misiyano.

Sploncytes Opolation

Sploncytes OpolationMaitiro ekubvisa masero emasero kubva kuguruve kuti ashandiswe muzvisikwa zvakasiyana-siyana zvekutsvaga, senge cytometry yekudzokorora, itsitokine yekugadzira, kana yakasanganiswa lymphocyte maitiro.

Iyo spune yakabviswa pasi pemamiriro ezvinhu ezviitiko, pasi pekuzviparadzaniswa uye yakaendeswa kune yakasvibirira tube ine rpmi 1640 pakati. Iyo lukocyte layer yakanangana nekukasira mushure mekunge centrifugation. Iwo masero akagezwa neRPMI 1640 pakati, centrifugred uye supernatant yakaraswa. Iyi yekugeza nhanho yakadzokororwa 1 - 2 times asati masero ari ega asati asvika aishandiswa kuedzazve kuedza.

Freezing Sploncytes

Freezing Sploncytesinhanho yakakosha kune kuchengetedza masero ekushandisa mune ramangwana. Crispresdersvation inobvumira vaongorori kuti vachengetedze Splonowytes kwenguva yakareba vasingakwanisi kukanganisa kwavo kana kuita kwavo.

Iyo supernatant yeiyo tsime centrieted cell kumiswa kubva kune yekuzviparadzaniswa padanho rakaraswa uye sero rekusweretesa rakanyimwa mudenga repakati. Wedzera ana aliquot kune yega yega tube tube uye kuendesa kumudziyo wekutonhora, unoona kuti masero anoramba ari kumiswa. Kukurumidza kusunungura midziyo yemahara mu-- 80 ° C frezer. Chinjana kusvika - 150 ° C Freezer Conter (kana mvura nitrogen tank) kwenguva yakareba -

Thawing splonowcytes

Thawing splonowcytesInofanirwa kuitwa nokungwarira kuti ive nechokwadi chekodhi yakakwira uye inoshanda mushure mekushandiswa kweklkpreservation. Iyo thawing maitiro kazhinji inokurumidza kuderedza zvinokuvadza zvinokuvadza zve dmso uye ice crystal yekuumbwa.

Makisheni anoendeswa kusvika pakugezera kwemvura 37 Wedzera 0.5 - 1ML yeCell Custure Svikiro kune chando tube, resendender uye kuendesa kumiswa kune 15ml tube yakazadzwa necersict tsika svedzero. Centrifuge, bvisa supernatant uye tora iyo tube kuti isunungure gadziriro yesero. Wedzera 1ML yeCLECE CLECE Svikiro, Kurova uye zvakare Resepend nePipiette, wedzera svikiro kune vhoriyamu yegumi nemashanu ml. Centrifuge, de Supernatize, Wedzera 1ML yeCell Custure Svikiro, kuwedzera svikiro maererano nekutarisirwa kutarisirwa cell. Isa masero muCo2 incubator uye incubate ye1h ine zvishoma gidhi muvharo. Pakupera kweIndenderedzwa, resendepend uye siya iyo 1min kubvumira iyo yakaunganidzwa sero sero kuti isarure. Kumiswa kwesero pasina kunyatso kuendeswa kune itsva 15ml tube. Masero akaverengerwa kuti atarise chiitiko chechitokisi.

Mhedziso

Splonowste, neyakajairika mharidzo dzakasiyana uye chiitiko chekukosha mumhinduro dzedziviridzo, zvishandiso zvinoshamisa mukutsvaga kusingaitwi. Kunyangwe akatorwa kubva pamatanda, epamusoro, kana mamwe marudzi emazimbe uye imbwa, splocytes inofambisa zvidzidzo zvakanyanya mukudzvinyirira muBismive, zvirwere zvechirwere, uye cancer kuvandudza, uye cancer immunotherapy. Maitiro ekuzviparadzanisa nevamwe, uye kusununguka, uye tywing zvakakosha kuti uchengetedze kutendeka uye kusanganisa, kugonesa vaongorori vekuongorora masimba masimba emhando dzakasiyana siyana. Sekunzwisisa kwedu kuenderana nekudzikira, kushandiswa kweslenocytes kucharamba kuchiramba kuchingotanga kukwezva mishonga pakati pezvidzidzo zvepamberi uye zvinosimbisa kufambira mberi mumushonga wevanhu uye neveterinary.