Chii chinonzi PBMCs yevanhu uye basa ravo mukushanduka?

PBMC yemunhuS, kana Peripheral Ropa Sonaclear maseru, iboka rinorema remasero emagetsi anotenderera neropa rako. Aya masero anosanganisira lymphocytte, monowt mitengo, uye masero emafaro, mumwe nemumwe achitiridza yakasarudzika basa mukudzivirira muviri wako. PBMCS inokuchengetedza nekuziva uye kusarerekera hutachiona hunokuvadza, kunge mabhakitiriya nemavhairasi, uchiramba uchigumira zvisina mwero.

Tsvagurudzo inoratidza PBMCS inoramba yakagadzikana nekufamba kwenguva, ine sero kubhadharisa pamusoro 85% mumazhinji samples. Uyezve, kutaura kwadzo gene kunogona kujairana nekukurumidza. Semuenzaniso, tontatation kutaura kunowedzera neanopfuura matatu mukati memaawa. Uku kuenderana kunoenderana nekuratidza kukosha kwavo mukudzora mhinduro dzedziviriro.

Kuumbwa kwePBMCS yevanhu

Lymphocytte: T Masero, B Masero, uye Masero eNK

Lymphocytte chikamu chakakosha chePBMCs yevanhu. Ivo vanosanganisira t masero, b masero bs, uye mhondi dzekare (NK) masero, rimwe nerimwe riine mabasa akasarudzika mukushanduka. T masero anobatsira kudzora mhinduro dzekudzivirira uye kurwiswa zvakananga masero. B masero eB anogadzira antibodies, ayo anotora vatengesi anokuvadza. NK masero, kune rumwe rutivi, chinangwa uye kuparadza masero asina kusimba, akadai seaya anotapukirwa nehutachiona kana masero emanyowa.

Sezvineiwo, mamwe lymphocytes muPBMCs yevanhu, anozivikanwa seCD3 + CD19 + maseru, anoratidza maitiro maviri. Aya masero anogona kuita seese masero tese uye masero b. Ivo vanopindura kutyisidzira kuburikidza ne t - cell receptor (TCR) uye B - cercor (bcr) kusaina nzira. Iyi nhare mbiri inovatendera kuti vatore chikamu mune zvese zvinonakidza uye celluly immune mhinduro. Semuenzaniso, vanosunga antigens zvakanyanya kupfuura zvinowanzoitika pamasero anowanzoitika uye kuburitsa Interferon - Gamma (ifn - γ)

Monocytes uye mabasa avo ekudzivirira

Monocytte ndeimwe boka rinokosha mukati mePBMC. Aya masero anotenderera kweropa rako reropa, achitsvaga zviratidzo zvehutachiona kana kukuvara kwematehwe. Kana vangova nedambudziko, vanotama kunzvimbo yakakanganisika uye vanoshandura kuva Macrophages kana masero emafirita. Macrophages Erulf uye kugaya matanda, nepo maseru anokosheswa aripo anonamira kune mamwe masero emuviri.

Monocytte inoburitsawo cytokukorodzano, ayo ari kusaina mamorekuru anobatsira kuronga mhinduro dzekudzivirira. Nekuita izvi, ivo vanoona kuti muviri wako unonyatsonaka kutapukira kana kukuvara.

Masero emaoko uye basa ravo mukuratidzira

Masero emagetsi ane hunyanzvi anyanzvi antigen - Kuburitsa masero (APCS) mukati mePBMC yevanhu. Vanotamba basa rakakosha mukuvandudza t masero nekuburitsa maantigens pane yavo. Tsvagurudzo inoratidza kuti masero emagetsi ndeaya ega maApcs anokwanisa kuendesa ese CD4 + uye CD8 + NADE T Masero. Kubudirira kwavo kunobva pakukwanisa kwavo kudzora antigen digention, inowedzera kuwanikwa kweeptides yeMHC yekuisa.

Aya masero anoona kuti immune yako system inoziva uye inopindura kutyisidzira zvinobudirira, kuita kuti ive yakakosha mukununurwa.

Kuzviparadzanisa kwePBMCS yevanhu

Masosi ePBMCS: Peripheral Ropa uye pfupa marongo

PBMCS yevanhu inogona kuve yakasarudzika kubva kune maviri ekutanga zvinyorwa: Peripheral Ropa uye pfupa remombe. Ropa rePeripheral ndiyo yakajairika sosi nekuda kwekuwanikwa kwayo uye kushoma kwekupinda. Bone marongo, kune rumwe rutivi, inopa nzvimbo yakapfuma yemasero isina kusvibiswa asi inoda maitiro anoshan'arira.

Iyo goho uye kuchena kwePBMC dzinogona kusiyana zvichienderana nekwainobva uye kusarudzika nzira. Semuenzaniso, ongororo dzinoratidza kuti standard ficoll nzira inowana gohwo rakakwirira uye kucheneswa zvichienzaniswa neCPT (cell kugadzirira tube) nzira. Iyo tafura iri pazasi inoratidza iyi misiyano:

Ficoll pamusoro pehunyanzvi hwePBMC kusarudzika

Iyo Ficoll Overlay Delly maitiro inzira yakashandiswa zvakanyanya yekuparadzanisa PBMCS. Kuita uku kunosanganisira kudzidzisa ropa pamusoro pe ficoll - Paque Solution uye centrikuging iyo yekuparadzanisa masero zvichienderana nedensity. PBMCS inoumba musiyano wakasarudzika pakati plasma uye ficoll, kuvaita nyore kuunganidza.

Zvidzidzo zvinosimbisa kukosha kwekubata kwakakodzera panguva iyi yekuita kuti ive nechokwadi. Semuenzaniso, imwe fundo yakawana kuti kushandisa ficoll nenzira kwayo kunogona kuzadzisa kuchena kweinosvika makumi masere neshanu muzana pamwe kusiyana kushoma. Iyo tafura iri pazasi inofananidza nzira dzakasiyana dzekupinda dzePBMC OPENYU:

Immunmumastic kupatsanurana nzira

Immumunomagnetic kupatsanurana ndeimwe nzira yepamberi yekuparadzanisa PBMCS. Iyi nzira inoshandisa magnetic beads akabatanidzwa neantibodies ekutarisa chaiyo marudzi echitokisi. Kugadzirisa kurongeka kunoparadzanisa masero nekuzvisunga kumahofisi, nepo kuronga kwakashata kunobvisa maseru asingadi, kusiya vanhu vavanoda.

Tsvagurudzo inoratidza kuti kusarongeka kwakashata kunochengetedza masero okuswereta uye hakukanganise mamakisi ekugadzirisa senge IL - 2r (cd25). Mukupesana, kurongedza kwakanaka kunogona kuderedza kusimudzira uye activation kugona, kunyanya mushure mekukurudzirwa. Tafura iri pasi apa inopfupisa izvi zvakawanikwa:

Iyi nzira inonyanya kubatsira paunenge uchinyanya kuda sero cell mafuro ekutsvaga kana ekurapa mashandisirwo ekushandisa.

Zvikumbiro zvePBMCS mukutsvaga uye mushonga

Basa mumota - T Cell Therapy Budiriro

PBMS yePBMC inotamba nzvimbo inokatyamadza mumotokari inofambira mberi Aya masero anoshanda seyavakutanga zvinhu zvekugadzira mota - masero t, ayo ari mainjinwi kuti afanane nekuparadza cancer masero. Unogona kushamisika kuti zvinobatsira sei pbmcs mune izvi maitiro. Zvidzidzo zvinoratidzira zvinofadza mhedzisiro:

• Mushure memazuva gumi nemaviri etsika, 1 × 10 ^ 7 Frozen PBMCs inogona kuburitsa kanenge 1.48 × 10 ^ 9 Mesocar - T masero, ane masero anopfuura makumi matatu muzana.

• Cytoxicity bvunzo dzinoratidza mesocar - t masero anotorwa kubva kutsva uye akachengetedzwa vePBMCLED yakafanana nenzira yakafanana. Pane mahedzisiro -

• Kunyangwe pane yakaderera ratio ye2: 1, hapana musiyano wakakosha muCytotoroligity inoonekwa.

Izvi zvakawanikwa zvinoratidza kuvimbika kwePBMC mukugadzira mota inoshanda - T masero, kunyangwe mushure menguva yakareba.

Shandisa mune zvinodhaka zvinodhaka uye zvine chepfu zvidzidzo

PBMCs inokanganisika mune zvinodhaka zvinodhaka uye toxicity zvidzidzo. Ivo vanopa munhu - yakakodzera muenzaniso kuti uongorore kuti zvinodhaka zvinobatana nemasero emuviri. Semuenzaniso, vatsvagiri vakaedza iyo mishonga quinacrine pane pbmcs kuti vaongorore kupfeka kwayo. Tafura iri pasi apa ichipfuura zvakawanikwa:

Mhedzisiro iyi inoratidza kuti pbmcs inopindura kushingairira kubvisa, kunyangwe pazinga dzakaderera. Izvi zvinovaita chishandiso chakavimbika chekufanotaura mhinduro dzekudzivirira kumishonga mitsva.

Biomarker kuwanikwa uye kudzora maumenewing

PBMCs akakosha kune kuzivisa biomarkers uye kuongorora hutano hwako. Biomarkers inoyera zviratidzo zvezvikamu zvebhizimusi kana chirwere. Nekuongorora PBMCS, iwe unogona kuvhura biomers iyo inoratidza immune chiitiko, kufambira mberi kwechirwere, kana kushanda kwekurapa. Semuenzaniso, vatsvagiri vanowanzo kuyera mazinga eCytokine muPBMC ekutarisa mhinduro dzevhu panguva yekutakura kana marapirwo. Iyi nzira inobatsira kurapa kurapwa kune varwere vega, kuvandudza zvibodzwa.

PBMC inogonesa zvakare kureba - Term Kugadzikana kwavo uye kushandurwa kwavo kunovaita kuti vave vakanakira kuteedzera shanduko mukudzivirira kwemuviri kwenguva. Izvi zvinonyanya kubatsira pazvirwere zvisingaperi kana panguva yekurapa kwenguva refu.

PBMCS yevanhu inokatyamadzwa mukunzwisisa uye kufambira mberi kuchengetedzwa. Kuumbwa kwavo kwakasiyana-siyana-lymphocytte, monowt mitengo, uye masero emafaro anovaita kuti vaite mabasa ekutsoropodzana. Kusarongeka Kwematekinorozi seye FICOLL Overlay uye Immunomagnic Kupatsanurana Unoona Kuti Unogona Kuwana Yakakwira - Kuchena PBMCS yekutsvaga kana kuronga kushandisa.

Mafaro avo anowana huwandu hwakawanda hweminda:

• Zviuru zvezvidzidzo zvakashandiswa pbmcs mukutsvaga kwekiriniki mumakore makumi mashanu apfuura.

• Ivo vakakosha kune mota - T cell kurapa, kukura kwezvinodhaka, uye kudzivirirwa kwemhinduro yekuongorora.

• PBMCS inobatsira pakuwana biomarker, kugadzikana kwemurwere, uye kusagadzikana kwechirwere chekutsvagisa.

Nekusimudzira pbmcs, iwe unogona kuvhura mikana mitsva mukudzivirira uye kukudziridza kurapa kwakashata kunoshandura hutano hwehutano.

FAQ

Chii chinonzi pbmcs chinoshandiswa pakutsvaga kwekurapa?

PBMD inobatsira vaongorori vekudzidza Impu mhinduro, kuyedza zvinodhaka mitsva, uye nekukudziridza marapirwo semota - T Cell kurapwa. Kujaira kwavo uye kusimba kunovaita ivo vakanakira kuyedza izvo zvinoda masero emunhu emunhu.

Unochengetedza sei PBMCs yekushandisa mune ramangwana?

Iwe unogona kuchengeta PBMCs neClyopreserves yavo mumvura nitrogen. Iyi nzira inochengetedza yavo yekubatanidza uye kushanda kwemakore, ichikubvumira kuti uvashandise kare - term zvidzidzo kana zvekushandisa.

PBMCS yakafanana nemasero machena eropa?

Kwete chaizvo. PBMCs iri subset yemasero machena eropa ayo anosanganisira lymphocyte, monowt mitengo, uye masero emafaro. Ivo havabvumi magirazi kunge neutrophils, iyo zvakare chikamu chemasero machena eropa.

Ko PBMCS inogona kushandiswa kudzidza autoimmune chirwere here?

Ehe! PBMCs yakakosha pakufunda autoimmune chirwere. Ivo vanokubatsira kuongorora zvisina kuchema maitiro, kugadzirwa kweCytokine, uye genetic mamakisi, kupa ruzivo rwekunzwisisa kuita michina yemarango uye zvinogona kurapwa.

Ko PBMC inopararira chiitiko chakaomarara here?

Kwete saizvozvo. Maitiro akadai seye ficoll overlay nzira kana kuti imunomagntic kupatsanurana anoita PBMC kusazviparadzaniswa kwakananga. Nekudzidziswa kwakakodzera uye michina, iwe unogona kuwana yakakwira - kuchenesa samples yekutsvaga kwako.