Zvigadzirwa zvePhase
|
Chigadzirwa Zita |
Kutaurwa |
|
Phase munhu wemvura |
1ml |
|
1ml |
|
|
1ml |
|
|
1ml |
|
|
Iphase shedhi (New Zealand White) Mhedzisiro Fluid, Yakasanganiswa Gender |
1ml |
|
Iphase Rat (Sprague - Dawley) Mvura yemvura, murume |
1ml |
|
Iphase rat (sprague - dawley) mvura yemvura, mukadzi |
1ml |
|
1ml |
|
|
Hippake vanhu vitreous kuseka, murume |
1ml |
|
1ml |
|
|
1ml |
|
|
Nyaradzi rePhepeti (New Zealand White) Vitreous kuseka, murume |
1ml |
|
1ml |
|
|
Iphase Rat (Sprague - Dawley) Vitreous Humor, Chirume |
1ml |
|
Iphase Rat (Sprague - Dawley) Vitreous kuseka, Mukadzi |
1ml |
|
50ml |
|
|
Phepake Apticher Vitreous Humor |
50ml |
Liquid chromatography - Tandem misa spectrometry (lc - ms / ms)
Mvura chromatography - tandem misa spectrometry (LC - MS) Inyanzvi ine simba inoongorora iyo inosanganisa mukurumbira wekuparadzanisa kwemvura chromatography nekugona kwehuwandu hwekuongororwa kweTandem misa spectrometry. MuLC - MS, ms musanganiswa unotanga kuparadzaniswa nemvura chromatography zvakasiyana nechikamu chechiteshi uye chimedu chekuparadzaniswa navo pavanenge vachipfuura nepakati penhokwe. Izvo zvikamu zvakaparadzaniswa zvinobva zvanyatsoongororwa uye kuongororwa neTandem misa misa spectrometry, izvo zvidimbu zvigadzirwa zviri muzvinyorwa zvigadzirwa zvechigadzirwa.
Zvishandiso zveLC - MS / MS muBioanalysis
Bioanalysisinosanganisira kuyerwa kwekutarisisa kwezvinodhaka, metabolites, uye mamwe mishonga yekubereka mukati memasampulogical samples, senge ropa, plasma, urini, uye zvimweBiofluids. LC - MS inonyanya kunaka - inokodzera izvi zvekushandisa nekuda kwekunzwa kwayo kwakakwirira uye kugona kuona zvakaderera zvekutarisa kweanenge kuongorora matrices akaomarara.
LC - MS / MS tekinoroji yekuongorora kwezvinhu zvebhaibheri sampulogy anoona zvese zvine utsinye uye zvakawedzera zvinhu. Vaongorori vakafungidzira samples chaiyo nekuwedzera chinhu kuti chiyerwe kuna aBlank Matrixkuumba huwandu hwehuwandu hwekudzora sampuli uye mhando yekudzora mhando. Iko kusunganidzwa kwechinhu icho kuve kuyerwa mune yebenzi sampuli inogadziriswa neyakajairwa curve.
Zvinhu zvakawedzera zvinhu zvinhu zvinoitika zvakasarudzika mumuviri. Chinhu chinopfuma - Zvinodhaka zvine chekuita nezvakakosha kutungamira kwekusimudzira kwezvinodhaka mumakore apfuura. Pamwe chete nekuzvarwa kwehuwandu hukuru hwezvinodhaka nezvinhu zvakawedzera, iyo bioanalysis yemishonga ine zvinhu zvinowedzera zvine chekuita zvakanyanya uye zvakatonyanya kukosha. Nekudaro, parizvino, kusimbiswa kwezvinhu zvekuongorora zvekuongorora nezveFDA uye mamwe madhiri ekudzokorora zvinodhaka zvinonyanya kutarisa pazvinhu zvisina kujairika, kusanganisira kunyatsoziva, matrix, chiyero chekudzorera, uye kugadzikana. Kubvira pakuonekwa kwezvinhu zvinowedzera zvinotungamira kumatambudziko mukuonekwa mhedzisiro nekuda kwekuita kwayo kana kuwana isina chinhu kutevedzera iyo chaiyo sampuli, iyo kubuda kweimwe nziraBlank Biological Matrix (Yakasarudzika isina biological matrix) inogadzirisa dambudziko iri.
Tafura 1: Tsananguro yeKusarudza muindasitiri's Finstream Bioanalytical Nzira Dzidziso Yechokwadi
|
|
Ema Bmv |
FDA BMV |
Ich m10 BMV kutungamira |
Pharmacopeia yeVanhu Verence of China 2020 edition |
|
Diki molecule |
Kusarudzwa kunofanirwa kuratidzwa uchishandisa zvisingasviki 6 yemunhu oga masosi ezvakakodzera zvisina musoro matrix, ayo ari mumwe nemumwe akaongororwa uye akaongororwa kuti apindire. |
Iye anotsigira anofanira kuongorora samples dzakakodzera dzeBiological matrix (i.e.e.e.......g Kupura) kubva kunhanharo matanhatu (yeCCS). |
Kusarudzwa kunoongororwa uchishandisa blank samples (matrix samples yakagadziriswa pasina ana ana ana ana ana ana kana) yakawanikwa kubva kune angangoita matanhatu emunhu masosi / mijenya uye isiri - Lipaemic). Kusarudzwa kunofanirwa kuongororwa mumirimo ine mirapu uye haemoly ses samples. |
Kusarudzwa kunofanirwa kuratidzwa uchishandisa yakakodzera svivi substrates kubva kune angangoita 6 zvidzidzo (mhuka isina chinhu matrix inogona kusanganiswa mumabhandi akasiyana) |
|
Macromolecule |
Kusarudzwa kunoedzwa nekupuruzira kanokwana gumi masosi emuenzaniso wematanho pane kana padyo neLloo. |
Iye anotsigira anofanira kuongorora samples dzisina kufanira dzezvakakodzera biological matrix (i.e.e.e........ELPLASE) kubva kune gumi (yeLBAS) yemunhu manhamba. |
Kusarudzwa kunoongororwa uchishandisa zvisina mwero samples dzakawanikwa kubva kune imwe gumi emunhu masosi uye nekupedza iyo yeuua. Blank matrices kuLloo uye kune yakakwira OC Level. Kusarudzwa kunofanira kuongororwa mumifananidzo yemirimo uye kusarudzika kwemasampuli. |
Kusarudzwa kunofanirwa kuongororwa nekuwedzera kuongororwa neyekupedzisira uye kwepamusoro lints |
Ongororo nzira yekuvandudza uye yekuongorora nzira yekusimbisa
MuBioanalysis, inovimbisa kuvimbika uye kubereka kwemhedzisiro yekuongorora ndeyekunyaradza. Izvi zvinoda kukura kwakasimba uyekusimbiswa kwekuongororwanzira.
Ongororo Nzira YekuvandudzaInosanganisira kusikwa kwemaitiro akakodzera ekuona kuti kuongorora uye kuongorora kuongorora kufarira. Izvi zvinosanganisira kusarudza iyo yakakodzera chromatographic mamiriro (i., stationary chikamu, chiyero cheMS paramende (ionization) kuti uwane kurongeka kwekunzwa Pamusoro pezvo, nzira yacho inofanirwa kuve inokwanisa kunyatsoongorora kuongorora kwezvinhu zvakaoma uye zvinoshanduka zvezvipfuyo zvematroni, izvo zvinowanzoumbwa nemapuroteni, LIPIDS, uye mamwe mishonga inogona kukanganisa kuongororwa.
Kamwe nzira yakagadzirwa, inofanira kuitwaOngororo Nzira IndeationKuve nechokwadi chekuti inosangana neyekufunga maitiro ekuita. Iyi nzira yekusimbisa inofanirwa kuratidza kuti nzira yacho yakakodzera chinangwa chayo chinangwa uye inoenderana nezvinowanzoita zvinodiwa. YeBioanalytical Nzira, kusimbiswa kazhinji kunosanganisira akati wandei kiyi parameter:
- - Kunyatsojeka uye kunyatso:Kuona kuti nzira inopa chaiyo uye isingachinji mhedzisiro.
- - Sensitivity:Kugona kuona kusanzwisisika kwekutanga kwekuziva.
- - Sarudza:Iko kugona kwemunzira kusiyanisa ana ana ana ana kubva kune mamwe makomendi mune matrix.
- - Kudzoreredza:Kubudirira kunowanikwa newave kunobviswa kubva mumuenzaniso wekubereka.
- - Kugadzikana:Kugadzikana kwekunyorera pasi pemamiriro akasiyana uye ekubata mamiriro.
- - Nhengo:Iko kugona kweiyo nzira yekugadzira mhedzisiro iyo yakananga zvakananga kune iyo autangere yekuongorora pamusoro peiyo yakatarwa renji.
Isina biological matrix uye isina matrix matrix inotamba zvakaoma mabasa mune ino yekubvumika maitiro. Aya ekudzora samples, ayo asina ana ana ana ana aumbwa, akakosha pakuzivisa zvinogona kuitika zvematongerwo enyika kana kukanganiswa panguva yekuongorora. Ivo vanobatsira kumanikidza baseline mazinga ekuongorora uye anoona kuti matrix pachayo haapi mupfuure kune kusaina kusvibiswa kana kudzvinyirira. Zvakafanana, kushandiswa kwezvinodhaka - Mahara Matricesinokosha yekuteedzera kuti hapana zvinodhaka zvakasara kana metabolites aripo mumuenzaniso uyo ungangodaro uchipwanya mibairo.
Bioanalysis yemishonga yeOfhthalmic
Madziro eziso rakakamurwa kuita matanda matatu, iyo yekunze gayer ndiyo minraro simbi; Iyo Middle membrane ndiyo pigment membrane, vascular membrane kana uvea; uye iyo yemukati membrane ndiyo retina. Iro ziso rakakamurwa kuita zvikamu zviviri, matunhu eAntherior uye epamberi eziso, akasungwa nemusana we lens.
Mufananidzo 1. Anatomy yeziso revanhu.
Zvivakwa zvikuru zvinosanganisirwa mushonga metabolism zvinosanganisira:
- Cornea- Iyo yekutanga nzvimbo yezvinodhaka zvekutakura zvinodhaka, ine denthanges uye cytochrome p450 (cyp) enzymes iyo metabolize pradruggs.
- Conjunctiva- Mupfumi mumushonga - metabolizing enzymes (i., leadses uye cyps), inopa yekutanga - Pass metabolisption isati yasvika.
- Kuseka kwakanaka- Limited metabolic chiitiko asi anotamba chikamu mukugoverwa nemishonga uye clearance.
- Vitreous- Intravitreal jekiseni inogona kuita zvakananga pane retina uye kuderedza hutachiona mukupararira kweSomatic. Diki mishonga ye mishonga inosiyana nekukurumidza, uye mishonga yakakura yemamorekuru ine imwe hafu yechisere - hupenyu. Vitreous shanduko nezera rinokanganisa mapestacokinetics.
- Sclera- Sclera inogamuchirwa kune yakakura mishonga mishonga uye ndima yezvinodhaka kuburikidza neSclera inonyanya kukanganiswa neiyo size. Subconjunctivation jejejende dzinobvumira zvinodhaka kupinda iyo Chorid, asi maitiro akaomarara. Scleral Melanin inosungira mushonga uye inokanganisa kuburitswa kwayo uye nguva yekuita.
- Posterior ziso reziso- Iyo retroocular tishu dzakapfuma muropa kuyerera uye zvinodhaka zvinogona kubviswa kuburikidza nekukasira muviri kana kuti Lymph. Choroidal Vascular Hyperpermeability inobvumidza zvinodhaka kuti ipinde nyore kuti ipinde nzvimbo yekunze, asi zvakaoma kuyambuka pigment yePithanina, iyo inokanganisa kushanda uye kunotungamira pakurasikirwa. Melanin - Kusunga Zvinodhaka kunogona kuwedzera iyo nguva yekuita.
Kuseka kwemukati uye vitreous kuseka
The thekuseka kwakanakauyeVitreous kusekaIzvo zvakakosha kuti zvidzorerwe zvehunyengeri zvinotamba mabasa akaoma mukuchengetedza inyonganiso, kupa zvinovaka muviri, uye kufambisa kujekesa kwemhepo. Iko kuseka kwemvura ndiyo yakajeka, yakajeka, mvura yemvura inozadza zvese zvinopedza uye zvepamhepo makamuri eziso, ane ion, mapuroteni, makarote emakona, uye oksijeni. Vazhinji vemo vane ruzivo vanogadzirwa neiyo ciliary vemuviri, vanobuda ziso pakona rakaumbwa nejendi reIris neCornea. Iyi mvura inosiyana mumhando dzakasiyana, kusanganisira vanhu, nyani, tsuro, uye vamwe vasiri - primate. Ivo vaiwanzounganidzwa vane huwandu hwakakura hwakawanda kubva kumhuka dzemunhu kana madziva.
Kuseka kwemvura kuyera mhando
Kuseka kwemunhu munhu
The thekuseka kwemunhu munhuiri rakajeka, rine hutano Iyo inogadzirwa nemudiwa wechibharo uye inoyerera kuburikidza neakamuri yeAnterior usati wadurura kuburikidza nehuremu hwetsamba.
Monkey kukumbira kuseka
The themonkey kukumbira kusekainonyatsofanana neiyo yevanhu mukuumbwa uye simba. Yakapihwa kufanana kweanatomical pakati pemukuru uye vanhu,Kwete - vanhu vanoomesa moyoinoshanda seyakakosha kutaurwa kweOphthalem zvidzidzo.
Tsuro inomuka
The thetsuro inomukainosiyana zvakanyanya kubva kune iyo yemapurazi, kunyanya mune yayo protein yekuvhara uye chiyero chekutsvaira. Matsime anowanzo shandiswa mukutsvaga ocular, kunyangwe zvisikwa - zvakanangana zvakasiyana zvinofanirwa kutariswa.
Vitreous kuseka kuyera mhando
Kuseka kwevanhu kwevanhu
The thekuseka kwevanhu kwevanhuIcho chigero - sechinhu chakanyorwa kunyanya chemvura, collagen, uye hyaluronic acid. Iyo inochengetedza chimiro chegungano, inobata mabwe, uye inoshanda seyakabhadharwa yekufambisa noutrient.
Monkey vitreous kuseka
The themonkey vitreous kusekainogovera kuumbwa kumwechete kune vanhu vitreous kuseka, kugadziraNon - Humi Yemunhu Mutambi Vitreous HumorMuenzaniso unonakidza wekudzidza nezera - Yakanangana neVitreous Degeneration uye inoenderana mapatani.
Rabit Vitreous Humor
The theRabit Vitreous Humoryakarongedzwa zvakasiyana, kuve yakawanda mvura - kunge uye kuve nekuderera collagen density. Misiyano iyi inokanganisa kupindura kwayo nekuvhiringidza kwekuvhiya uye pharmacological kurapwa.
Kuvandudzwa kweiyo yekunyepedzera uye yakatemerwa ocular fluids
Kuzvidza mhereke uye yekuzvinyudza kumuka kuseka kuseka
Kusekerera kwemukatiuyeKugadzira Vitreous Humoritsimendi inomhan'arira yakagadzirirwa kushandiswa muOphthalmic kuvhiya, kutumira zvinodhaka, uye mafomu ekutsvaga. Aya maitiro emvura anotevedzera biochemical uye epanyama zvivakwa zvavo zvezvisikwa.
Sururated mapogen uye yakatemerwa vitreous kuseka
Simudziridzo inoshatiswauyesimpated vitreous kusekaIS LABURatory - Yakagadzirirwa Mhinduro Dzinoshandiswa muVitro kuyedza uye modelling ocular physiology. Vanofambisa zvidzidzo zvinodzorwa pasina manyorero ehunhu hwakabatana nemhuka kana samples yevanhu.
Mhedziso
Iko kushandiswa kwemvura chromatography - Nzira yekunzwa kwakakwirira, kurongeka, uye kusarudzika kunoita kuti ive inokosha mukuyera uye endogen body bodys, kunyanya mune yeOphhthalem zvinodhaka. Kunzwisisa kwakadzama kweOcular Anatomy uye basa reMvura yakadai sekumukira uye kuseka kushungurudzika kunosimbisa kukosha kwezvinhu zvekutumira zvinodhaka. Uyezve, kukura kwekunyepedzera uye kwakarongedzwa ocular fluids furters yekutsvaga mukana uchiona kufunga kwetsika kunosangana. Sezvo ongororo yekuongorora inogara ichishanduka-shanduka, inovimbisa kuvimbika uye kubereka kwakakodzera kune zvinoshanda zvekiriniki zvinobudirira, kunyanya muOphthalmology.
Mazwi akakosha: LC - MS / Blank Biological Matrix, STOPLICK YEMAHARA, BOIOFICE YEMAHARA, Humi Yemunhu Humori Humori, Humi Humori Humor, Monkey Vitreous Kuseka, sungura kuseka kuseka, kusiri - vanhu vanotsauka zvinosemesa,Yakatemerwa kukwirira kuseka, yakafungidzirwa kuseka kuseka, kuseka kwemvura, kuseka kwemukati, kuseka kwekunyepedzera kuseka.
Reference
Seyerpour, S. M., vakapara, L., Rezazadeh, G., & Ricken, T. (2023). Mathematical modeling yemhinduro yemamiriro ekusagadzikana kweiyo isingabvumirwe yakadziviswa capacitive glaucoma glaucoma sensor.Kuyerwa: Sensors, 30, 100936. Https://4doi.org/10.1016/J.Measen.2023.100936
Kutumira Nguva: 2025 - 03 - 26 - 26: 2:35