Bile iyo xayawaanka biin-danaa ee matrix bayoolaji oo madhan loogu talagalay habka loo yaqaan 'bioanlyticalt' Horumarinta iyo habka loo yaqaan 'BILANTALLICALL'

1 Alaabada iPhope

2 Bianix Blank ah

Noocyo kala duwan oo ahMatries bayoolaji, oo ay ku jiraan maran oo dhan dhiig, serum, plasma, bile, kaadida, kaadida, saxarada, xubnaha goobaha, kaftanka vitrebrous, kaftanka aqueresson, iwm.

3 Baahida loo qabo in la isticmaalo maran bayoolaji bayoolajiga ah ee daraasadaha hordhaca ah

In Cilmi baarista daawada daroogada, matrix bayoolojinadu waa aasaaska aasaasiga u ah sameynta iyo xaqiijintaQaababka falanqaynta bayoolojiga. Adigoo adeegsanaya matros maran oo ka yimaada maaddooyinka caafimaadka qaba ama xayawaanada tijaabada ah, cilmi baadhayaashu si sax ah ayey u diyaarin karaan Heerarka Diyaargarowga iyo saameynta saameynta tayada leh, saxeedka, iyo matrix, iyo matrix, oo ay hubiyaan kalsoonida tirada tirada tirinta daroogada.

4 Habka noolaha bayoolojiyada ansaxinta iyo aasaasida iyo adeegsiga matrix bayoolojiyadeed maran

Sida ay u baahan tahay Tilmaamaha FDA / Tilmaamaha EMA, samaynta iyo ansaxinta hababka falanqaynta bayoolaji, matashadu bayoolajiyada bayoolaji ayaa loo baahan yahay in loo isticmaalo in lagu hubiyo saxnaanta iyo isku halaynta habka falanqaynta. Gaar ahaan LC - Falanqaynta MS / MS / Ms Falanqaynta, kala duwanaanshaha qaybaha matrix ee ilo kala duwan ayaa si weyn saameyn ugu yeelan kara hufnaanta ionization. Matrix maran Ficilka iyo saameynta matrix ee habka ogaanshaha ayaa u baahan shuruudo sare ee matrix madhan.

5 Sawirro codsi oo ah matrix madhan oo madhan oo ku saabsan horumarinta daroogada

Matrix batrific aan dabiici ahayn (matrix oo lamid ah)waa xalka isku-darka ee ku dhinta qaybaha bile ee dabiiciga ah waxaana loo isticmaalaa dheef-shiid kiimikaadka daroogada, toxicology-ka, iyo baaritaanka bayoolojiga.Bile matrix madhanWaxay leedahay qiimeyn codsi oo muhiim ah xaaladaha soosocda ee ka dib: Daraasadaha dawooyinka ee soosocda ee dawooyinka nidaamka hepatobiliary, qiimeynta is-dhexgalka dheef-galeenka, iyo horumarka hababka baarista ee dawada, iyo hababka farta baalaga ah (LC - Ms / MS).

6 Dawooyinka Nidaamka Nidaamka Hagaasujirka

Horumarinta daroogada ee nidaamka hepatobiliary, bile matrix madhan ayaa ka ciyaara kaalin aan la beddeli karin oo jaangooyooyin ah oo ku saabsan nidaamka partro-ka faylka vitroppanininetic (PK), sunta ah, baaritaanka bayoolojiga, iyo baaritaanka bayoolaji.

6.1 Qiimeynta astaamaha wasakhda ah ee daroogada

Cilmi-baaris ku saabsan wareegga xiidmaha Adigoo adeegsanaya matrix aan dabiici ahayn oo aan dabiici ahayn

Go'aaminta Heerka Tusmada Bile: Dawooyinka si firfircoon uga soo baxa bile (sida sabuurad iyo antibiyootigyo gaar ah), bile aan dabiiciga ahayn (bile oo aan dabiici ahayn) ayaa kaa caawin kara in ay caddeeyaan hufnaanta hufan oo ay u sahliyaan resiment resiment.

6.2 Baro saamaynta acids-ka bile ee galka daroogada

Saamaynta Shaqo-xumida

Isdhaxgalka Dawooyinka Bile ee Daroogada

6.3 Qiimaynta halista ee hepatotoxicity iyo cholestasis

Daroogada ayaa laga helaa Moodeel Cholestasis

Falanqaynta BioMarker: Adigoo ku daraya qaybo gaar ah sida bilirubin, acids bile, iyo waxyaabaha bararka, saameynta daroogada ee qaybaha bile waa la qiimeyn karaa oo badbaadada caafimaad ayaa la saadaalin karaa.

6.4 Faa'iidada anshaxeed iyo wax ku oolka ah ee lagu beddelayo bile dabiiciga ah

Wax ka qabashada arrinta muunadda muunad-yareynta

Isku-darka Dufcaddu

6.5 Taageer horumarka LC - Ms / Ms Place Falanqeynta

Saxitaanka Saamaynta: Halabuurka murugsan ee bile-ka dabiiciga ah (cusbada saliida sare, fosfooraska, midabka) ayaa si sahlan u horseedi kara xakamaynta ion ama kor u qaadista LC / Falanqaynta Ms / Ms. Matrix Bile oo aan dabiici ahayn (oo ah matrix 'oo lamid ah matrix-ka

7 Cilmi-baarista noocyada iskutallaabta

Halabuurka bile wuxuu si weyn ugu kala duwan yahay xayawaanka kaladuwan (jiirka, eeyaha, daanyeerka), iyo bile aan dabiiciga ahayn waxay qiyaasayaan kala duwanaanshaha noocyada iyo hagaajinta tirinta macluumaadka tixraaca.

7.1 Cynomolgus daanyeerka bile (NHP bile)

Bile of cynomolgus Daanyeer aad u badan ayaa ka mid ah dadka aadanaha marka loo eego astaanta (sida BSPORT Profile Progst (sida PRESS PRESS Mousanalka aan ahayn - Isdhaxgalka Propatic Standard Excretion Ifcibar iyo Isdhaxgalka Bile ee Daroogada.

7.2 Beagsheeg bohol eey

Waxyaabaha fosfooraska ah ee ku jira beaggeegu waa mid aad u sarreeya, taasoo ku habboon barashada wax lagu barto bileyga iyo milixda cusbada leh ee ku tiirsanaanta daroogada lipophilic. Si kastaba ha noqotee, farqiga u dhexeeya ka kooban aashitada bile ee u dhexeeya beagsheega iyo aadanaha waxay u baahan yihiin wax lagu dhejiyo iyada oo loo marayo matricooyinka macmalka ah.

7.3 SD Rat bile

Mugga bile ee ka soo ururinta jiirka SD waa mid aad u ballaaran Si kastaba ha noqotee, waa in la ogaadaa in guntooda bilirubin ee sarreeya ay faragalin karaan LC - Falanqaynta Ms / Ms.

7.4 ICR / CD - 1 Mouse Bile

CD - 1 Mouse waxay leeyihiin mugga yar yar (50 - 200 l l), iyaga oo ka dhigaya ku habboon barashada qaababka moodooyinka wax ka badalida hiddaha (sida Fxr Wadada FxR / PXR-ka), laakiin farsamooyinka furitaanka micro-ga ayaa looga baahan yahay la qabsiga.

7.5 C57bl / 6 jiirka bile

C57BL / 6 Mouse waxaa badanaa loo isticmaalaa moodallada cudur dheecaanka, iyo isbeddelada ku dhaca halahooda bile waxay ka tarjumaysaa xaaladaha cudurada, ee u baahan adeegsiga matrix aan dabiici ahayn ee baalashiyanka si loo barbar dhigo cudurka cilmiga ah.

Moodooyinka kor ku xusan waxay u baahan yihiin in lagu daro matrix aan dabiici ahayn oo aan dabiici ahayn si ay u xakameeyaan kala-duwanaanta noocyada ah waxayna kor u qaadaan kala-duwanaanta iskutallaabta isbarbardhiga LC / Ms hababka Ms / Ms.

Gabagabo

Matrix ee aan dabiici ahayn, aalad muhiim ah oo loogu talagalay in lagu beddelo shaqada bile ee dabiiciga ah, waxay door muhiim ah ka tahay horumarka dawooyinka hepatobiliary. Anigoo jaangooyo ka kooban noocyada kaladuwan ee noocyada kaladuwan (sida eeyaha cynomolgus, eeyaha cd, cd), cd jiirka, iyo jilitaanka asaasiga ah ee muunadaha dabiiciga ah, iyo jilitaanka asaasiga ah ee muunadaha dabiiciga ah, oo si weyn u xoojiya isku halaynta iyo qiimaha tarjumida ee cilmi baarista daroogada.

Iyada oo ay ku jiraan horumarinta dawada saxda ah, matrix aan sax ahayn ayaa ku dari doona qaybo gaar ah oo ku saabsan tikniyoolajiyadda gaarka ah si ay si firfircoon u dhigto beerka iyo xameetida microenamides, oo siinta taageero xoog leh ee horumarka daroogada ee hal-abuurka leh.

Erayada muhiimka ah:Falanqaynta bayoolojiga ah, muunad bayooloji, falanqaynta bayooloji, bile bile, muunad xayawaan, bile oo aan dabiici ahayn, ms / Ms - Ms / Ms / Ms / MS / MSALLICATITITS HESHIISKA

Qoraal ka warbixineya

Li t, chiang jy. Difaaca Bile Acid ee Cudurka dheef-shiid kiimikaadka iyo daaweynta daroogada. Dawooyinka dawooyinka 2014 oct; 66 (4): 948 - 83. Doi: 10.11224 / Pr.133.008201. PMID: 25073467; PMCID: PMC4180336.