Hepatocytes ee dheef-shiid kiimikaad daroogada ah iyo sirimaha maandooriyaha ee Sirna: Qiimaynta Nidaamka Model ee Noocyada

FIIRO GAAR AH: XAALADAHA HIRPATOCYYTES waxay u baahan tahay in loo isticmaalo dhexdhexaad dhalaalaya iyo dhexdhexaad; Xannaaneynta xargaha ee la isticmaali karo ayaa loo baahan yahay in loo isticmaalo in lagu isticmaalo isbadal, Xarun dhexdhexaad ah, dhexdhexaad dayactir, iyo saxanka colaten ee colateden. Celceliska Cym-ka waxay u baahan tahay MRNA Indiction Qalabka Assayd sidoo kale.

Hepatocytes

HepatocytesWaa unugyada waxqabadka koowaad ee beerka, oo door ka mid ah ka ciyaara dheef-shiid kiimikaadka, ka-saarista, waxsoosaarka bile, iyo condotesis-ka borotiinka. Waxay sameeyaan qaab dhismeedka iyo bayoolajiga ee shaqada beerka, oo u shaqeeya dhexdhexaadiyeyaasha guud ee is-beddelka iyo xeryahooda kala duwan ee walxaha waxyeellada leh iyo kuwa waxtarka badan ee maandooriyaha iyo sunta. Awooddan ujeeddadan ee hepatocytes-ka si loo habeeyo kiimikooyinka waxay ka dhigtay wax aan la-galeyn karin labadaba jaangooyooyin caafimaad iyo cilmi baaris labadaba, gaar ahaan marka la baarayo wadooyinka ku lug leh dheef-shiid kiimikaadka daroogada iyo sunta beerka.

Hepatocytes aasaasiga ah

Heepocytes-ka aasaasiga ah wuxuu tixraacayaa unugyadaas oo si cusub looga go'doonay unugyada beerka - ha ahaato xayawaan ama xayawaan aan lahayn ku dheelitiran faafinta vitro. Sababtoo ah unugyadani waxay helayaan wax badan oo ah waxqabadka enzymatic iyo howlaha jir ahaaneed ee astaamaha beerka ah, waxaa badanaa loo tixgeliyaa heerka dahabiga ah ee daraasadaha baarista beerka (nashaadaadyo gaar ah. Isticmaaladooda cilmi-baaristu waa muhimka si ay u fahmaan xasilloonida dheef-shiid kiimikaadka iyo awoodda go'aan qaadashada ee daroogada si ay u maraan isbadal ama ay ku sii jiraan qaabkooda asalka ah ee lagu soo rogi karo nidaamka bayoolojiga.

Xargaha heesaha iyo hakinta hepatocytes

Qaab-dhismeedka tijaabada ah, hepatocytes ayaa laga dheereeyn karaa iyadoo lagu saleynayo xaaladooda dhaqan-galinta. Qalabka hepatocytetes-ka ee la isticmaali karo waa kuwa u hoggaansanaanta substrate dhaqanka, kaas oo u saamaxaya lifaaqa xasilloon iyo morphology oo ku dhowdahay jawiga vivo. Xiriirkan kaliya maahan inay taageerto qaab dhismeedka loo qoondeeyay ee unugyadan laakiin sidoo kale waxay ku adkaynaysaa muddo dheer - ereyga daraasadaha ee wadooyinka bayoolajiga ah sidaCym450 enzyme jimicsiga. Waxaa taa ka duwan, eryida hepatocytes-ka waxaa lagu hayaa kuwa aan laheyn Dhaqamada cayrintu waxay fududeeyaan soo-gaadhista labis badan ee unugyada si ay u tijaabiyaan xeryahooda, taasoo ka dhigeysa inay ku habboon yihiin qiimeyn deg deg ah ee xasilloonida dheef-shiid kiimikaadka iyo jawaabaha hepatotoxic ba'an.

Xargaha xargaha

Xargaha xargahawaa unugyo beerka go'doonsan oo ku saleysan suxuunta qaaska ama takhasuska gaarka ah. Xiriirkan ayaa u oggolaanaya unugyada inay ku faafayaan, inay soo baxaan, oo ay sameeyaan xiriiriyeyaal is-dhexgal ah, oo inbadan ku xafidaya vivo - sida shaqooyinka badan ama xitaa toddobaadyo. Sababtoo ah unugyadaasi waa feejignaan, waxaa loo isticmaali karaa in lagu dabagalo jawaabaha daba-dheeraada, Cym450 enzyme waqti ka dib, iyo dad badan oo sun ah. Qalabka hepatocytetes-ka ah ayaa sidoo kale sii wadaysa si xoog leh loo yaqaan 'cell' Nooc kasta - ha ahaato qof bini-aadam ah, oo aan ahayn - samaxii dadka, jiirka, ama wax kale ayaa u baahan xaalado sawirro ah (sida jaakad gaar ah ama kaabisyo gaar ah) si loo beddelo xaaladaha wanaagsan ee jimicsiga.

Eryitaannada hepatocytes

Taas bedelkeeda,Eryitaannada hepatocyteswaxaa lagu abaabulay aan aan ahayn Xannaanooyinka Hangareeska ayaa ku habboon gaagaaban - tijaabooyin ereyga, sida daraasadaha dheef-galeenka dheef-galeedka daroogada ama qiimeynta degdega ah ee enzymesics. Maaddaama unugyada aaney ku xirneyn lifaaqyada matrix, si dhakhso ah ayaa loo diyaarin karaa loona isticmaali karaa imtixaanno shaqeynaya iyadoon dib loo dhigin looga baahan yahay u hoggaansanaanta unugyada. Dhaqamada hakinta ayaa si gaar ah waxtar ugu leh marka la barbar dhigo noocyada noocyada noocyada ah, maadaama ay u oggolaanayaan mid caadi ah oo caadi ah oo ka mid ah ganaaxyada HOPatocytes ee aadanaha, non - jiirka, iyo noocyada kale.

Xasilloonida dheef-shiid kiimikaadka iyo is-hoosaadka

Deganaanshaha dheef-shiid kiimikaadkaMacnaha guud ee cilmi baarista hepatocyte waxaa loola jeedaa awoodda molecule, badiyaa murashax daroogo, si looga hortago isbadalka biochemalial ee by enzymes beerka. Baadhitaanka deganaanshaha dheef-galeenka oo leh hepatocytes ayaa u oggolaaneysa cilmi-baarayaal inay saadaaliyaan kala badh - nolosha iyo soosaarka burburka ee walxahaas, oo ay muhiim u tahay horumarka daroogada. Profile-ka dheef-shiid kiimikaad ee xarunta ayaa go'aamin kara oo keliya wax ku ool ah laakiin sidoo kale nabadgelyadeeda, maaddaama metabolites uu mararka qaar soo bandhigi karo saamaca sunta ah ee aan lagu arkin xarunta waalidka.

Hepatotoxicity, dhinaca kale, waxay ka kooban tahay waxyeelada waxyeellada leh ee walxaha kiimikada ee unugyada beerka. Isticmaalka labada aasaasiga ah ee asturatocytes ee daraasadaha sunta rasaasta waxay ka caawisaa inay ka saaraan farsamooyinka u horseeda dhaawaca beerka. Tani waa mid muhiim u ah maxaa yeelay beerka, isagoo ahaanshaha xubnaha dheef-shiid kiimikaadka udub dhexaadka u ah, wuxuu u nugul yahay cayda sunta ah ee kiimikada deegaanka iyo wakiilada dawooyinka. Daraasadaha la adeegsanayo unugyadan waxay ogaan karaan qaababka burburka unugyada ama dhajinta, iyagoo siinaya fahamkooda dhacdooyinka hore ee dhaawaca beerka iyo ka caawinta saadaalinta natiijooyinka xun ee goob caafimaad.

Indhasha Enzyme waa qayb kale oo muhiim ah oo ku xiran shaqada hepatocyte, oo tixraacaya geedi socodka ay ku qoran yihiin maadooyinka qaarkood ee ay kordhiyaan isku-darka daroogada - metabolizine enzymes. Ifafaaledan ayaa si gaar ah muhiim u ah maadaama ay u horseedi karto dheef-shiid kiimikaad oo la kordhiyo Co; Cilmi baarista iyadoo la adeegsanayo hepatocytes platocytes-ka ah ayaa inta badan loogu talagalay in lagu baaro jidadka sharci-darrada ah ee xukuma jidadka enzyme, oo iftiimiya is dhexgalka isku dhafka ah ee ka dhexeeya isdhaxgalka kala duwan ee kala duwan iyo awooda dheef-shiid kiimikaadka beerka.

Helpatic Sirna si gaar ah iyo istiraatiijiyad bartilmaameed ah

Horumarka ugu dambeeyay ee faragelinta RNA (RNI) daweyn ayaa soo saartay istiraatiijiyado sheeko ahGeedka la bartilmaameedsadayadoo adeegsanaya faragelinta yar-yar ee rna (srinas). Qaab muhiim ah ayaa ku jira isku-dhafka moleekules strana-ka leh nAsgpr). Asgppr waxaa si weyn loo muujiyaa dusha sare ee hepatocytes wuxuuna door muhiim ah ka ciyaaraa gudaha endocytic-kaGalnac - SirnaWaxay ka dhigeysaa waddo muhiim u ah beerka - gaarsiinta daroogada qaaska ah.

Daraasadaha vitro ee sida caadiga ah shaqeeyaHeersamocyte Apatocyte Sirna SirnaFarsamooyinka si loo qiimeeyo hufnaanta iyo qeexitaankaSirina Galnac Heratocyte. Qaababkan ayaa u oggolaanaya cilmi-baarayaashu inay bartaan saameynta chonka ee hiddo-wadaha qaab ahaaneed oo ku habboon iyo in lagu saadaaliyo qaababka gaarsiinta ee ugu badan ee isgaraacinta ugu badan ee is-xakamaynta iyo hufnaanta hiddaha ee hidde-wadaha.

Guud ahaan, gaarsiinta la bartilmaameedsaday ee ku saabsan daweynta RNA iyadoo la adeegsanayo ASGPR - Maskaxda Galnac) Nidaamyada sicir-bararka iyo kuwa dheef-galeenka ah ee lagu daweeyo moodalka qaaxada iyo kuwa dheef-shiidka ah, waxay sii xoojinayaan utility-ka ah moodalka cilmi-baarista ee aasaasiga ah iyo horumarka daroogada.

Noocyada - Faahfaahinta XPatocyte

Hepatocytes aadanaha

Plapatocytes bini-aadamka waxay muhiim u yihiin saadaalinta dheef-shiid kiimikaadka daroogada aadanaha iyo sunta rasaasta, oo bixiya muhiimadda shaqeynta sare. Assiyadooda ku saabsan taarikada takhasuska leh waxay u oggolaaneysaa daraasadda in la istcimaalo dheef-shiid kiimikaad, wajiga i / II ee dheef-shiid kiimikaadka, iyo howlaha gaadiidka dadweynaha.

In aan loo baahnaynJoojinta hepatocytes aadanaha Si dhakhso leh uga jawaab Xonobiotics-ka, iyaga oo ka dhigaya inay ku habboon yihiin qiimaynta dheef-shiid kiimikaadka degdega ah, daraasadaha nadiifinta, iyo gaagaaban - imtixaanka sunta tox.

Non - hepatocytes-ka bini-aadamka

Isku ekaanshaha jir ahaaneed ee aadanaha ayaa sameeyaJeexjiirid daanyeerka cynomolgus monkey hepatocytesiyoJeexjiitlethes patsus daanyeer hepatocytes qiimo u leh barashada xabadka ee is-afgaradka natiijooyinka aadanaha. Waxaa loo isticmaalaa in lagu qiimeeyo dheef-shiid kiimikaadka daroogada, enzyme-ka, iyo noocyada - astaamaha amniga gaarka ah.

Joojinta cynomolgus Daanyeerka hepatocytesiyoJoojinta Rhesus daanyeer hepatocytes U oggolow si dhakhso ah, qiimeyn isbarbardhiga howlaha dheef-shiid kiimikaadka iyo jawaabaha degdega ah, waxtarka badan marka gaagaaban -

Canot hepatocytes

Ugu horreyntii ka faa'iideysan jiray cilmibaadhista xoolaha iyo tar-tareenka,Pat papatocytes eygaiyoSuuxdin beagle hepatocytesMIMIC ku jirta Viviology Piviology Piviology oo lagu baranayo dawada - oo ay sababtay dhaawaca beerka iyo dheef-shiid kiimikaadka dawooyinka xoolaha.

Kuwaani waxay bixiyaan daraasad dheef-ka-qaadis dhakhso ah oo loo shaqeeyo xilli hore - baaritaanka masraxa, oo ka faa'iideysanaya kuwa degdega ah ee tusaalaha hakinta.

SPRAGAE - Dawley Rat hepatocytes

WaxanPrapueuepue - Dawley Rat hepatocyteswaxaa laga goynayaa Sparaga - Rats Dawley waxaana lagu asaasay qaab feejignaan leh ama qaab dulsaar leh. Qaabka loo yaqaan 'platlet', unugyadu waxay ku dhegan yihiin saxanka dhaqanka, taas oo u oggolaaneysa inay sameeyaan is dhexgalka bulshada ee xasilloon oo ay joogteeyaan beerka iyo beerka - howlo gaar ah oo loogu talagalay muddooyinka fidsan. Qaabkani wuxuu si gaar ah ugu habboon yahay barashada muddada dheer - howsha dheef-shiid kiimikaadka teeramka, enzyme jimicsiga, iyo qiimeynta sunta ah ee sunta ah ee loo yaqaan 'spragara' -

Markii lagu hagaajiyo Aan ahayn Gobol Aan Laheyn -Xannaaneynta SPAGAGE - Dawley Rat hepatocyteswaxaa loo isticmaalaa ugu horeyn loogu talagalay gaagaaban - tijaabooyin ereyga. Qaabka hakinta ayaa fududeynaysa assys-ka deg-degga ah, sida Daraasadaha Dheef-ka Dheef-ka Dheef-ka Daroogada ee daran iyo Qiimaynta Enzyme Hegetics, halkaas oo looga baahan yahay jawaabaha waxqabadka ee degdega ah. Qaabkani waa mid faa iido leh marka la barbar dhigo jawaabaha dheef-shiid kiimikaadka ba'an ee u dhexeeya noocyada ama xaaladaha kala duwan.

ICR / CD - 1 Mouse Heatocytes

Ka go'day ICR / CD - 1 jiirar, kuwan ICR / CD - 1 Mouse Heatocytesu hoggaansanaanta saxanka dhaqanka, taasoo u oggolaaneysa inay sii wataan howlaha beerka muhiimka ah waqtiyo dheer. Baarayaasha waxay doorbidaan habka jiifka ee ICR / CD - 1 Mouse Heoucocytes marka xasilloonida, muddada dhaqan ee la dheereeyey ay lagama maarmaan u tahay indho-indheynta tijaabada ah.

Taa bedelkeeda, ganaax ICR / CD - 1 Mouse Hangacoodtes oo aan loo hoggaansanayn, sidaa darteed waxay sii hayaan qaab dhismeedka wareega ee ku habboon kuwa gaagaaban -Cayrinta ICR / CD - 1 Mouse HeaucocytesWaxay u oggolaaneysaa qiimeynta deg-degga ah ee jawaabaha dheef-shiid kiimikaadka waxayna si gaar ah waxtar ugu leedahay baaritaanka hordhaca ah ama markii sareeya - Tijaabada wax soo saarta ayaa loo baahan yahay. Adeegsiga eryitaannada Hanpatocytes ee ICR / CD - 1 Jiilka wuxuu ka caawiyaa inuu si dhakhso ah u dejiyo astaamaha dheef-shiid kiimikaadka aasaasiga ah isla markaana isbarbar dhiga noocyada kale ama xaaladaha tijaabada ah ee tijaabada ah.

Feline Hepatocytes

Qaabka loo yaqaan 'platlat' ayaa si gaar ah waxtar ugu leh muddo dheer - daraasadaha muddada-dheer ee u baahan beerka joogtada ah - howlo gaar ah, sida Qiimaynta sunta ah ee sunta raaga iyo tijaabooyinka enzyme-ka. Daraasaadka lagu saaray cudurada ku dhaca beerka ee ka dhaca beerka, daroogada - dhaawaca, iyo cilladaha dheef-shiid kiimikaadka ee gaarka ah ee bisadaha,Catch plat hepatocytesSii qaab adag. U hoggaansanaanta ayaa sidoo kale kor u qaadaya dib-u-soo-noolaynta horudhaca ah iyo kuwa shaqeynaya, iyaga oo ka dhigaya iyaga tusaale ah oo laga doorbido dawooyinka farmashiyaha iyo cilmi-baarista maskaxda.

Qaabka hakinta ayaa fududeynaysa cabirka unugyada unugyada ee degdegga ah ee Xenobiotics, halkaas oo la'aanta lifaaq la'aanta ay yareyso daahitaanka jawaabta ee lagu arko muddo dheer -Cayrinta Bit Hepatocytes Sidaa darteed muhiim u ah in la garto isbedelada biochemical-ka hore iyo falcelinta xun ee ka soo horjeedda ka dib soo-gaadhista xeryahooda cusub.

Digaag hepatocytes

Sida wakiilo ka socda moodooyinka avian,Digaagga is-jiifka ah ee loo yaqaan 'hypatocytes'Waxaa loo isticmaalaa daraasadaha isbarbardhiga si loo fahmo kaladuwanaanta horumarsan ee howlaha beerka, gaar ahaan qiimaynta carqaladeynta Endocrine.

Digaag digaag ah ee hepatocytesawood u siiya dhaqso, ba'an

Noocyada kale

Noocyada kale, Jiig tiripigenphig heatocytes, Cayrinta Minipig Hanpatocytes, Jeega oo la dul qaadan karo New Zealand White Rabbocytes, Joojinta New Zealand White White Rabbocytes Dhammaantood sidoo kale waa noocyo caan ah oo saadaalin kara iyo sharraxa daroogada - is dhexgalka maandooriyaha iyo barashada cilmiga cytotomicity ee daroogada.

Gabagabo

Hepatocytes wuxuu door muhiim ah ka ciyaaraa joogtaynta shaqada beerka ee dheef-shiid kiimikaadka, juqeynta, waxsoosaarka bile, iyo wax soo saarka bile, iyo isku-darka borotiinka. Maqaalka ayaa hoosta ka xariiqay farqiga u dhexeeya hepatocytes-ka aasaasiga ah iyo qaabkooda asaasida ah ee qaab-dhismeedka iyo hakinta hepatocytes-mid kastaa wuxuu ku habboon yahay ujeeddooyinka tijaabada kaladuwan. Xannaano-fadhiga oo la miisayn karo, oo aan la jaan qaadi karin oo soo ifbaxaya barashada saameynta daba-dheeraada, iyo kordhinta dheef-galeenka dheef-galeenka, inta lagu joojiyo xaaladaha imtixaanka ee isku-darka iyo isku-darka ee loo yaqaan 'Asey'. Intaas waxaa sii dheer, falanqayntu waxay ku fiddaysaa noocyada - codsiyada gaarka ah, oo ka tarjumaya sida kaladuwanaanta hipotocyte Models-lagana qiimeynayo nidaamyada dawada, iyo qaabeynta daweynta dawada, iyo naqshadeynta daweynta la beegsanayo sida wax ka qabashada daroogada iyadoo la adeegsanayo isku-darka Galnac. In dulqaadani dhameystiran waxay muujisaa doorka isku-dhafka ah ee hepatocytes labadaba cilmi baarista rugta caafimaadka iyo horumarka daruuriga, oo siiya aragtiyo muhiim ah oo ku saabsan jimicsiga jirka ee jirka iyo farsamooyinka hoos udhaca amniga daroogada iyo waxtarka.

Furaha: Hepatocytes, hepatocytes-ka, hepatocytes-ka, hakinta hepatocytes, hepatocytes deganaansho, hepatotoxytes, cypatocme-ka, cypymetes-ka ah ee Cyymolgus Daanyeerka (PHH), MEPATOCYS MANKESS (PCHYESSES), PCHYESSES. Xaraashka bini-aadamka ee Cynomolgus Daanyeerka Daanyeerka ee Cynomolgus Hankey. Hepatocytes, dahab dahab ah, xamuul xamuul ah oo hamster ah, minigig chinet hepatocytes, xayiraadda heesaha astaamaha, hakinta 'cynomolgus daanyeero'. Joojinta Rymomolgus Daanyeer, daanyeerka Hepatocytes, ergocytes-ka, hakinta Beatocytes hepatocytes, ganaax ku hakinta daad / cd - jiirka hepatocytes, eryida hepatocytes, ganaax dahabiga ah ee Siiriya, xannibaadda Feline Hepatocytes, Joojinta Minipig hepatocytes, hakinta New Zealand White White Rabbocytes, eryitaan digaag ah oo digaag ah,Asgpr, Galnac - Sirna, Sirna, Asaasiga ah Hepatocyte Sirna Sirna Galnac Galnac Hepatocyte,Gaarsiinta la bartilmaameedsaday ee isqarxinta.