Xarkaha 'hepatocytes' vs. Cayrinta Hanganada: Kala duwanaanshaha muhiimka ah iyo codsiyada baaritaanka daroogada

I. Hordhac

In horumarka daroogada iyo baaritaanka sunta toxicology, hepatocytes uu door muhiim ah ka ciyaaro qiimeynta dheef-shiid kiimikaadka iyo amniga musharixiinta dawada cusub. Dhexdooda,xargaha xargaha iyoeryitaannada hepatocytes Ku darso howlo kala duwan, taasoo ka dhigeysa doorashada udhaxeysa iyaga muhiimka u ah inay sax yihiin barashada vitro. Fahmitaanka farqiga u dhexeeya astaamaha iyo codsiyadooda waxay ka caawin karaan baarayaasha inay doortaan tusaalaha ugu habboon baahidooda gaarka ah.

IPhose biosciants, hoggaamiye ku saabsan realgists bayoolojino bayoolaji oo hal-abuurnimo ah, waxay taageertaa horumarka sayniska iyadoo la siinayo sareyso - tayada hepatocytes tayada ee codsiyada cilmi baarista. Kursida loo yaqaan 'Cell' -

Maqaalkani wuxuu baarayaa kala duwanaanshaha ugu muhiimsan ee u dhexeeya xargaha xargaha iyo hakinta hepatocytes, codsiyadooda gaarka ah ee baaritaanka daroogada, iyo sida loo doorto nooca saxda ah ee cilmibaadhistaada.

II. Waa maxay xargaha xargaha xargaha?

Qalabka hepatocytes-ka ee la isticmaali karo waa unugyada beerka ee leh awooda ku dhejiska dusha sare iyo qaab-kale, iyaga oo ka dhigaya kuwo ku habboon muddo dheer - ereyga barashada vitro.

Astaamaha Muhiimka ah:

• Awood u leh u hoggaansanaanta taarikada dhaqanka

Joogto beerka - Hawlaha gaarka ah ee waqtiyada dheer

Ku habboon barashada barashada enzyme-ka, sunta, iyo waxqabadka transporet

Codsiyada baaritaanka daroogada:

• Long - Marka loo eego Daraasaadka Dheef-ka Dheef-ka-Saadaynta Daroogada:Waxaa loo isticmaalaa in lagu qiimeeyo sida daroogada loo baaro waqti ka dib.

• Daraasadaha EnzyMme Induction:Qiimaynta sida ay daroogooyin saameyn ugu yeeshaan waxqabadka beerka enzyme.

• Baadhitaanka Hepatotoxicity:Aqoonsashada dhaawaca beerka ka imaan kara xeryahooda cusub.

• Cilmi-baarista waxqabadka ee gaadiidka:Barashada daroogada - Is dhexgalka maandooriyaha iyo hababka kor u kaca / hababka kor u qaadida.

III. Waa maxay xayiraadda eryitaannada?

Cayrinta hepatocytes-ka waa kuwa aan ahayn Waxay hayaan hawlo enzymatic ah laakiin ha ku dhejinin taarikada dhaqanka, taasoo faa'iido u leh qiimeynta degdega ah.

Astaamaha Muhiimka ah:

• Non - adkeyn, oo lagu dayactiray gobolka ganaax

• Ku habboon in gaagaaban)

• Bixi wejigii la isku halleyn karo ee I iyo Wajiga II Enzyme Xogta Waxqabadka
  
  

Codsiyada baaritaanka daroogada:

• Gaaban - oo ah daraasadaha dheef-galeedka daroogada daroogada dawada:Cabbirka sicirka nadiifinta daroogada.

• Sare Sare - Baadhitaanka Waxqabadka (HTS):Si dhakhso leh u tijaabiya xeryo badan oo loogu talagalay xasilloonida dheef-shiid kiimikaadka.

• Nacayb muujinaya:Aqoonsiga wadiiqooyinka enzyme ee ku lug leh dheef-shiid kiimikaad daroogada.

• Dawooyinka - Daraasadaha is dhexgalka daroogada:Qiimaynta kartida loo tartamayo ee loo yaqaan 'complolism' iyo awooda xakamaynta xakamaynta enzymanme.

Iv. Kala duwanaanshaha muhiimka ah ee udhaxeeya xargaha xargaha iyo hakinta hakinta

Kala duwanaanshahaani waxay muujinayaan muhiimadda xulashada nooca ku habboon ee hepatocyte nooca ku saleysan ujeeddooyinka daraasadda.

V. Codsiyada baaritaanka daroogada iyo cilmi baarista

Labaduba la isticmaali karo iyo ganaax hepatocytes waxaa si weyn loogu isticmaalaa cilmi baarista dawooyinka iyo toxicology-ka. Fahmitaanka codsiyadooda waxay xaqiijineysaa qaabeynta tijaabada ee ugu habboon.

2. Dheef-shiid kiimikaadka daroogada iyo farmasiiyada (DMPK):

• Xannaano Hagapatocytes-ka ah oo la isticmaali karo

• Cayrinta Hantroolka waxay awood u siisaa qiimeynta deg-degga ah ee dariiqyada dheef-shiid kiimikaadka.
  
  

Baadhitaanka toxicology iyo is-baarista hepatotoxicity:

• Qalabka hepatocytes-ka lagu isticmaali karo ayaa ka caawiya ogaanshaha dawada raaga - dhaawaca beerka ee uu sababay (dil).

• Cayrinta hepatocytes waxay qiimeeyaan cytototomicity-ka daran iyo jawaab-celinta dheef-shiidka.
  
  

Marxaladda I & Wajiga II Daraasadaha Dhiig-dheellitirka ee dheef-shiidka:

• Marxaladda 1aad Falcelinta (Oktion, dhimista) ayaa si hufan u falanqeeya iyadoo la adeegsanayo labada nooc.

• Marxaladda dareenka ee wajiga (gluconidation, sulfacation) badanaa waxay u baahan tahay hepatocytes platocytes.
  
  

Soosaarista iyo Daraasaadka Xayiraadda:

• Xargaha heesaha ah ee la isticmaali karo ayaa muhiim u ah Cilmi baarista lagu muujiyo ee Cyp Enzyme.

• Xannibaadda Xilliga Cayaaraha ayaa si dhakhso leh u siiya aragtiyo deg deg ah oo ku saabsan suurtagalnimada xakamaynta xakamaynta enzyMyme.
  
  

Sare - Baadhitaanka daroogada ee wax soo saarka:

• Cayrinta hepatocytes fududeynta weyn - oo ah baaraynta xarunta.

• Xannibaadda xiisaha leh ayaa ka caawisa daraasadaha farsamo ee faahfaahsan.

Vi. Sida loo doorto nooca Hepatocyte saxda ah ee cilmi baaristaada

Markaad dooranayso hepatocytes cilmi-baarista, dhowr arrimood waa in la qaddariyo:

Muddada waxbarasho:

• Long - Waqtiga Tijaabada Tijaabada waxay u baahan yihiin hepatocytes xargaha.

• Gaagaaban
  
  

Yoolalka tijaabada ah:

• Soosaarista, sunta, iyo barashada dawladaha waaweyn:Xargaha xargaha.

• Baadhitaanka dheef-shiid kiimikaadka iyo xannibaadda damaanada:Eratocytes.
  
  

Noocyada Xulashada:

• Hepatocytes aadanahaku habboonaanta caafimaad.

• Xayawaanka hepatocytes xayawaankaDaraasadaha si taxaddar leh.
  
  

Fresh vs. repatocytes-ka:

• Heesta hepatocytesJoogtee suuragal sare laakiin helitaanka xadidan.

• Hepatocytes hepatocytesSoo bandhig ku habboonaanta iyo soo saaridda.
  
  

IPhose biosciens ayaa bixiya Sare - tayo tayo leh oo la isticmaali karo iyo hakinta hepatocytes ee codsiyada cilmi baarista. Khabiirkayagu wuxuu hubiyaa in saynisyahannadu helaan degmo bayoolaji oo la isku halleyn karo oo shaqeynaya si ay u taageeraan daahfurka daroogada iyo baaritaanka badbaadada.

Vii. Gabagabo

Fahmitaanka farqiga u dhexeeya soo-galka heesaha iyo hakinta hepatocytes-ka ayaa muhiim u ah xulashada moodalka ugu wanaagsan ee baaritaanka daroogada iyo daraasadaha sunta rasaasta. Xannaanooyinka hepatocytes-ka ah

ISPASE biosciens, bixiye aamin ah oo ku saabsan reatololojiin bayoolojino bayooloji ah oo hal-abuur leh, ayaa bixiya sareeya Awoodda ballaaran ee R & D oo ah shabakad qaybinta caalamiga ah, waxaan hubineynaa in saynisyahannadu ay marin u heleen moodallo hepatocyte la isku halleyn karo si loo jaro - Cilmi baarista.

Macluumaad dheeri ah oo ku saabsan xulashada Hepatocytes-ka saxda ah ee cilmi baaristaada, booqo iPhose bioscials maanta.