Falanqaynta isbarbardhigaya ee unugyada moniucharal-ka-mononucharal ee go'doominta (PBMCs): dhammaan codsiyada noocyada ee tallaalka iyo cilmi-baarista daweynta

IWax soo saarka wajiga

Unugyada monicucleral-ka ee durugsan (PBMCs)

Unugyada monicucleral-ka ee durugsan (PBMCs)Waa dad heterogenenous ah oo unugyada iskaga ilaalista laga helo dhiigga durugsan, oo lagu garto hal oucleus. Waxay ka mid yihiin lymphocytes-sida unugyada 'T', unugyada B, iyo dilaaga dabiiciga ah (Nk) unugyada - iyo mooshinnada, halka laga reebo granulocytes (Eryulophils) iyo erythrocytes) iyo erythrocytes. PBMCS caadi ahaan waa lagu go'doomiyaa iyada oo loo marayo cufnaanta heerka ugu hooseeya iyadoo la adeegsanayo warbaahinta sida Ficoll, kaas oo kala saara unugyada ku saleysan booycy. Nidaamkan aan la-tagi karin ayaa u oggolaanaya helitaanka si sahlan oo u ah qaybaha difaaca jirka, sameynta PBMC-yada waxay qiimo badan tahay cilmi baarista tallaal iyo horumarinta daaweynta.

PBMC Isosoo jeedin

Cabbirka, dhibanaha iyo culayska gaarka ah ee unugyada kaliya ee unugyada nuclatited ay ka duwan yihiin unugyada kale ee dhiigga durugsan. Cudurrada gaarka ah ee unugyada dhiigga cas cas iyo qanjirka polytorphontrick wuxuu ka baxaa 1.080, in unugyada unugyada nuclaashadu ay yihiin qiyaastii 1.070, iyo in platelots ay tahay 1.030. Sidaa darteed, farqiga u dhexeeya culeyska gaarka ah ee unugyada dhiigga ee kala duwan iyo unugyada unugyada nucladaha ee loo yaqaan 'nuclatited' waxaa loo adeegsan karaa si looga takhaluso unugyada dhiigga ee kala duwan ee unugyada nucladaha ee gaarka loo leeyahay. Cudurka cufan ee cufan ee filotll centrifuverint-ka ugu fiican waa habka ugu badan ee loo isticmaaloPbmc go'doomintaiyo daahirinta. Ficoll waa dhagar badan, oo dhexdhexaad ah, oo leh celcelis ahaan miisaanka molikal ee 400,000, iyo marka cufnaanta ay ka badato cadaadiska osmotic-ka ee caadiga ah, sidaa darteed waxaa badanaa loo isticmaalaa PBMC. Sidaa darteed, waxaa badanaa loo isticmaalaa sidii xal u kala goynta kala goynta PBMC. Erythrocytes iyo granulocytes waxay leeyihiin culeys gaar ah oo gaar ah oo hoos udhaca hoose ee tuubbada tuubada ka dib koronto; Lymphocytes iyo Monocytes-ka waxay leeyihiin culeys gaar ah oo ka yar ama le'eg oo u dhigma cufnaanta gaarka ah ee xalka kala goynta, oo ku dul sabeeya dusha sare ee xalka kala goynta ka dib, oo qayb yar oo ka mid ah unugyada sidoo kale waa laga joojinayaa xalka kala saarida. Adigoo ka rajeynaya unugyada lakabka cad ee dusha sare ee dareeraha xalka kala goynta iyo si ku habboon u maydhista unugyada si kumeelgaarka ah, oo ah unugyo nuclatis ah, I.E., PBMC, PBMC waxaa laga goynayaa dhiigga durugsan.

PBMCs noocyo kala duwan

In aan ahayn - dhismayaasha aadanaha, gaar ahaan cynomolgus daanyeero iyo daanyeer PBMC, PBMC Cilmi-baarista ayaa si aad ah loogu qiimeeyaa sababa la xiriira jirbadaha jir ahaaneed ee ay la wadaagaan aadanaha. Cilmi baarista iyadoo la adeegsanayocynomolgus daanyeerka pbmciyoRhesus Monkey PBMCAyaa karti u yeeshay daraasado faahfaahsan oo ku saabsan qaababka difaaca, iyo daraasado fara badan oo adeegsanaya daanyeerka PBMC-ka ayaa hoosta ka xariiqay suurtagalnimadooda taarikhda ee immunitrapy. Isticmaalka Daanyeerka ee PBMC ee ku jira imtixaanka tallaalka, baaritaanka daroogada, iyo infakshanka difaaca waxay noqotay lafdhabarta labadaba oo ah cilmi-baarista iyo cilmi-baarista caafimaad.

Sidoo kale, baaritaannada ku lug leheeyga pbmcsi weyn wax ugu biirisay tallaalka xoolaha. Daraasadaha la adeegsanayo pbmc eeyga PBMC waxay iftiimisay wadiiqooyinka jawaabta difaaca ee canines, halka cilmi baarista ku lug lehCanane PBMCWaxay sidoo kale iftiimisay isbarbar dhigga iyadoo la raacayo shaqada difaaca aadanaha. Beeraha cudurada zoolotic, eeyga 'eyga Pbmc iyo Canoin Pbmc waxay bixiyeen xog muhiim ah Pathogen - Isdhaxgalyo is-dhexgal ah, oo xoojinaya doorka unugyada la socoshada iyo horumarka daaweynta cudurka. Isticmaalka soo noqnoqda eeyaha PBMC iyo Canane PBMC ee daraasadaha isbarbar dhiga ayaa hoosta ka xariiqay muhiimadda ay tahay nidaam tusaale ah.

Moodooyinka jihada, oo ay ku jiraanRat PBMCiyoMouse PBMC, dhaqan ahaan waxay safka hore kaga soo jireen cilmi baarista tallaal. Baadhitaannada iyadoo la adeegsanayo saacadda PBMC faahfaahsan ayaa ku siisay Xeerka Naftanka ee Xeerka Conferitation ee xaaladaha bararka ee daba-dheeraada, iyo adeegsiga ballaaran ee Mouse PBMC-da PBMC wuxuu muhiim u ahaa barnaamijka hidda-wadaha iyo daraasadaha farsamada ee isdhaafsiga iyo infakshanka. Baarayaasha waxay sii wadaan inay ku tiirsan yihiin labada jiir PBMC iyo Mouse PBMC si ay u sahamiyaan hababka tallaalka ee adag, iyagoo moodajiyayaal ka dhigeysa. Intaas waxaa sii dheer, is dhexgalkahamster pbmcMeelaha tijaabada ah waxay ku dartay cabir kale baaritaanno tallaal ah, maadaama hamster Pbmc PBMC ay si joogto ah u siiso aragtiyo sheeko ah oo ku saabsan fayraska fayraska fayraska iyo istaraatiijiyada lawska. Iyada oo Hamster PBMC uu dhameystiro cilmi-baarista ee Rat PBMC iyo Mouse PBMC, moodallada jiirka waxay si wada jir ah u sameeyaan aalad dhameystiran oo loogu talagalay daraasadaha difaaca.

Moodooyinka xayawaanka waaweyn ayaa sidoo kale kobciyay garoonka, oo lehMinipig PBMCKu ciyaar door muhiim ah oo ku saabsan barashada xabadka ee xabadka iyo codsiyada caafimaad ee rugta caafimaad. Isticmaalka minigig PBMC waxay cadeysay faa iidada dawada dib-u-cusboonaysiinta iyo cilmi baarista ku tallaalidda xubnaha, halkaasoo Minipig PBMC ay siiso meel ku habboon oo lagu tijaabiyo daweynta cusub. Waxaa intaa dheer, baaritaanno adeegsanayabakayle pbmcwaxay qayb ka ahaayeen soosaarka antibody-ka iyo daraasadda ciladaha autoimmune, oo leh cilmi baaris bakayle pbmc ah si joogto ah u soo saarta fikradaha qiimaha badan. Adiga oo adeegsanaya minigig PBMC iyo bakayle PBMC ee Tardem, cilmi baadhayaashu waxay qiimeyn karaan wax ku oolka iyo badbaadada tallaalka cusub ee tallaalka ee nidaamyada jirka oo dhan.

Feeline iyocat pbmcCilmi baaristu waxay ka xardhay wax ay ku taallo ay ku leedahay daraasadda infekshannada dib-u-dhaca iyo ciribta difaaca. Baadhitaanno adeegsiFeline PBMCHasthumceenna ka ah fahamka nidaamka difaaca feline, halka daraasaadka ay la yeeshaan Cat PBMC-ga PBMC-ka ay ku soo bandhigaan jawaabaha difaaca ee la xidhiidha caabuqa fayraska. Labada faylka PBMC ayaa si isdaba joog ah loogu muujiyey baaritaanka sahanka ka dhexeeya falsafadaha ka dhexeeya falsafadda iyo dawooyinka difaaca ee soo noqnoqda ee Feline PBMC iyo Cat PBMC-da

Chorologyo anshaxeed Avian wali kama tagin,digaagga pbmciyoDuck PBMCDaraasadaha siiya aragtiyo xasaasi ah oo ku saabsan qaababka difaaca ee shimbiraha. Cilmi baarista PBMC waxay shaaca ka qaaday dhinacyo gaar ah oo ka mid ah jawaabaha u gaarka ah ee jawaabaha difaaca ee Avian, halka baaritaannada ku lug leh DBMC ay sii xoojisay fahamkayaga caabbinta ee loo yaqaan 'Cilmiga caabbinta loo yaqaan' patterin ' Isticmaalka soo noqnoqda ee digaagga PBMC iyo DBMC PBMC Baadhitaanka Baadhitaanka Infekshinka fayras iyo waxtarka tallaalka ayaa muujinaya muhiimadda ay u leeyihiin xakameynta cudurada ku dhaca xayawaanka iyo dadka bina aadamka.
Intaa waxaa sii dheer, daraasadaha la adeegsanayoGuinea doofaarka pbmcWaxay udub dhexaad u ahayd cudurka qaaxada iyo cilmi baarista tallaalka, halkaas oo doofaarka Guinea PBMC PBM ay si joogto ah ugu adeegtaa tilmaamayaal lagu kalsoonaan karo ee jawaabta difaaca. Isbarbar dhiga, baaritaanno ku lug lehAlpaA pbmciyoGeel pbmcwaxay soo jiidatay dareenka sababtuna tahay qaab dhismeedka antibody ee u gaarka ah ee ku jira noocyadan. Cilmi baaris ku lug leh Alpa PBMC iyo Geel PBMC ayaa soo saartay codsiyada daweynta sheeko, iyadoo labadaba Alpac PBMC iyo Geela Pbmc in lagu sahamiyo teknolojiyadda cusub ee nanoboddy-ka iyo difaaca -

Tabarucaadkabovine pbmciyoIdaha PBMCilaa tallaalka beeraha sidoo kale waa cajiib. Daraasadaha lagu sameeyo Boving PBMC waxay ku bixisay insiyo qoto dheer oo ku dhaca naaso-marantio cagaalo iyo cudur neef-mareenka, iyo daraasad balaaran oo adeegsanaya bovine PBMC si toos ah ayey u wargelisaa kor u qaadida caafimaadka lo'da. Si isku mid ah, isticmaalka soo noqnoqda ee adhiga PBMC ee baaritaanka tallaalka ayaa hodan ka gartay fahamkayaga jawaabaha tilmaanta, iyo baarista PBMC ee PBMC waxay sii wadaysaa hagida horumarinta talaalka waxtarka leh iyo borotokoolka maaraynta cudurka.

Doorka cilmi baarista difaaca

PBMC waxay ku dhex dhici kartaa jawiga vivo waxayna ku siin kartaa wax ku ool ah oo ku saabsan nooca cudurka vitro ee loogu talagalay horumarinta daroogada. Markii la fiiriyo saameynta daroogada ee ku saabsan faafitaanka PBMC, Apoptosis iyo Cytotomicity, waxtarka iyo badbaadada daroogada ayaa marka hore la qiimeyn karaa. Adigoo baranaya saameynta daroogada ee ku saabsan bartilmaameedyada waxqabadka iyo wadiiqooyinka is-qoritaanka ee PBMC, waxay kaa caawineysaa in la helo bartilmaameedyo cusub oo talaabo daroogo ah, natiijooyinkaanna waxay bixin karaan fikrado cusub iyo tilmaamo ku saabsan horumarinta daroogada.

Codsiyada ka hortagga antibody - Unugyada ku-tiirsanaanta - dhexdhexaadinta cytotomicicity (adcc)

Codsiga PBMC ee daahfurka daroogada waxaa matalayaKa hortag ah - Qolka ku-tiirsanaanta - dhexdhexaadinta cytotomicicity (adcc). Daawada ka hortagga - Unugyada ku-tiirsanaanta - dhexdhexaadinta cytototomicity, maadaama magacu ku tiirsan yahay, ka hortaga antibody - Dilka loo yaqaan unugyada lagu dilo unugyada bartilmaameedka ah. Habka qaaska ah waa la fahmi karaa sida soo socota: Qeybta FAB-ga ee antibodyd-ka waxay kuxirantahay antigen-ka sicirka loo yaqaan 'antigenic' oo ku saabsan qolka bartilmaameedka. Qaybta FC ee ka hortaga 'antibody' waxay ku xidhnaataa dusha sare ee loo yaqaan 'fcr dusha sare ee unugyada NK (maadaama unugyada NK ay yihiin unugyada' NK ', tan waxaa loo istcimaalaa tusaale ahaan), sidaa darteedna waa la sii daayaa, iyo sifeyska iyo Granzyme waa la siidaayaa. Dareemka iyo granzyme-ka ku dhex gala qolka lagu sameeyay xuubka tubrane ee bartilmaameedka ah oo sababaya unugyada bartilmaameedka ah inuu ku dhinto anigoo ku dhaqaaqaya apoptosis - enzymes la xiriira.

Codsiyada gudahaOthER TILMAAMAHA CAAFIMAADKA CARRUURTA

• - Falcelinta Lyphocyte ee isku dhafan (MLR)

Unugyada aasaasiga ah ee DC-da iyo ts-ka waa kuwo ku jira nidaam loo isticmaali karo in lagu qiimeeyo DC - dhexdhexaadinta dhaqdhaqaaqa unugyada. Unugyada DC waxay muujiyaan PD - L1 tiro aad u badan oo dusha sare ah, oo ku xidha PD - 1 dusha sare ee unugyada T 'si ay u xakameeyaan. Haddii daroogada ay si wax ku ool ah u horjoogsan karto dariiqa falcelinta ee u dhexeeya labada, waxay kicin kartaa faafitaanka unugyada t oo ah tiro aad u badan, sidaas darteed waxay xaqiijinaysaa waxqabadka waxqabadka ee daroogada.

- Therachivations asssizations

T - dhaqdhaqaaqa unugyada unugyadu waa mid ka mid ah ass-ka sida caadiga ah loo isticmaalo in la qiimeeyo saamaynta daroogada tallaalka. Iyada oo ku dhexjirta unugyada unugyada, in unugyada vitro ee loo yaqaan ''

- T-ka faafinta unugyada

T - Assyidations-ka faafinta unugyada waxaa loo isticmaali karaa in lagu baaro musharixiinta dawooyinka dawooyinka ah iyo in la qiimeeyo waxtarka daroogada iyagoo isbarbar dhigaya shahaadada loo yaqaan 'Tription' ee daroogada kala duwan - Kooxaha lagu daaweeyo ee kooxda kantaroolka ah

- Staphylococcal Entotoxin B kicinta PBMCs

Staphylococcal Entetotoxin B waa mid Supertatigen si toos ah u xiraya mhc mhc mhc mhc mhc - soo bandhigaya unugyada waxaana loo soo bandhigayaa T - ee soo-digaha ogeysiiska. Staphylococcal Entotoxin B wuxuu awood u leeyahay in aan laheyn - si gaar ah u shaqeysta T - unugyada oo uu ku dhiirrigeliyo siideynta cotokines, sida IL - 2, Il - iwm.

Doorka PBMC ee ka dhaca syndrome siidaynta Cytokine

PBMC waxay door muhiim ah ka ciyaaraysaa horumarkaCytokine siidaynta(CRS), oo ah jawaabta caabuqa halis-ku-dhaca ee nolosha ee nolosha ee nolosha. Markii lagu kicinayo - in tallaalka loo yaqaan 'thimtrapies' sida unugyada gawaarida, unugyada ka hortaga monoclonal, ama caabuqyada-unugyadan, si dhakhso leh ayey u qarsoonaayeen noocyo badan oo cytokokiines sida Il-6, il-1, Ifn-α, il-1, iyo tnf-1. Soosaarkan Cytokine-ka ee xad-dhaafka ah wuxuu kicin karaa xabaashii dhacdooyinka oo ay ka mid tahay qandho, baxsi xidhaa, iskaa wax u qabso, iyo digaag xubnaha badan. Fahmitaanka ku-biirinta PBMCs ee CRS waxay horseeday horumarinta waxqabadyada la beegsaday, sida IL-6 Xaraashka 'Reliptor', in laga caawiyo yareynta cudurka 'conzord's cronder duufaanka ceyrta ee la doonayo.

Conqalad

Marka la soo koobo, maqaalkani wuxuu hoosta ka xariiqay doorka furaha ah ee PBMCs uu ka ciyaaro hormarinta is-fahamkaya ee ku saabsan qaababka difaaca ee noocyo badan oo kala duwan oo noocyo badan ah. Adoo eegaya tabarucaadka gaarka ah - oo ka imanaya moodooyinka sarurka ah ee muraayadaha jir ahaaneed ee jirka ee loo yaqaan 'phiepologs' ee jirka, iyo nidaamyada avian ee ay soo bandhigaan faa iidooyin isbarbardhig ah oo aanu u beddelin oo keliya sida ay u socdaan jidka loo maro istiraatiijiyado hordhac ah. Isdhaxgalka PBMC Cilmi-baarista PBMC waxay ku tagtaa meelo kala duwan sida horumarka tallaalka, tijaabada daroogada, iyo sahaminta wadiiqooyinka cusub ee kamusha ayaa ka dhigaya doorkeeda lama huraanka ah ee labadaba sayniska aasaasiga ah iyo codsiyada caafimaad. Ugu dambeyntii, iskutallaabta falanqaynta PBMCs ee PBMCS waxay dib u xaqiijinaysaa qiimaha models unugyada unugyada sida loo tarjumi karo daaweyn wax ku ool ah oo ku saabsan caqabado fara badan oo tallaabooyin badan.

KEYOSS: Qaybta Moorteral Dhiiga ee Dukaanka, Cynomolgus daanyeerka pbmc, Rhesus Monkey PBMC, Eeyga pbmc, Canane PBMC, Rat PBMC, Mouse PBMC, Jid PBMC, Hamster PBMC, Minipig PBMC, Bakayle pbmc, feline pbmc, Cat pbmc, Digaag pbmc, duck pbmc, Guinea doofaarka pbmc, AlpaA pbmc, Geel pbmc, bovine pbmc, Idaha PBMC, Ka hortag ah - Qolka ku-tiirsanaanta - dhexdhexaadinta cytotomicicity (adcc), Cytokine sii-daadashada, PBME PBMC PBMC, PBMC,Go'doominta PBMC, Pbmc pbmc, Waxbarasho PBMC, LEKOPAK, Leukapheresis pbmc, HLA ayaa ku qoran PBMC.