Dan Adam Mononukister na mutum (PBMC)
PBMC na iya miraic a cikin - yanayin vivo don samar da ingantaccen aiki - Vitro Model ga Ci gaban Magunguna. Nazarin tasirin magunguna a kan maƙasudi da sanya hannu kan hanyoyin da zasu iya taimakawa sababbin kwayoyi, da kuma binciken don ganowa da kuma ci gaba.
Babban kwatancen aikace-aikacen PBMC a cikin ci gaban ƙwayar ƙwaƙwalwar ajiya sun haɗa da:
1.Ancawoly - tantanin halitta mai dogaro - Growited Cytotoxicity (ADCC): PBMC tana taka muhimmiyar rawa a ADCC; Kwayoyin NK, Macrophage, da sauransu sune babban sakamako pbmcs a cikin aikin ADCC. Wadannan sel zasu iya kashe sel na manufa Excordulated da rigakafin rigakafi ta hanyar ɗaure musu.
2.Mixed lymphocyte dauki (MLR): A CO - tsarin al'ada don sel na farko da sel sel da za a iya amfani da su don kimanta dokokin DC - Sun yi sulhu a cikin kunnen sel. Kwayoyin DC sun bayyana adadi mai yawa na PD - L1 a farfajiyar su, wanda zai iya ɗaure shi da ke hana PD - 1 a farfajiya na sel. Idan miyagun ƙwayoyi na iya toshe hanyoyin da suka dace tsakanin su biyun, to zai iya tayar da yaduwar sel kuma don haka tabbatar da aikin aiki na miyagun ƙwayoyi.
3.Tukan kunshin tantanin halitta: Tare kayan aikin tantanin yau da kullun suna ɗaya daga cikin assia na yau da kullun don kimanta tasirin magunguna na ƙwaƙwalwar ƙwaƙwalwar ƙwaƙwalwar ƙwayar cuta. A cikin Tallace-aikacen Tareds, a cikin - galibi ana amfani da ƙwayoyin Termuniya da kuma ƙwarewa mai dacewa, kamar maganin da suka dace, kamar maganin rigakafi ko rigakafi, ana ba shi don sa a sa ku kunna sel.
4.t proliverational prvife assay: Za a iya amfani da proluveration prvife Assay don nuna tasiri na kwayoyi ta hanyar abubuwa daban-daban - bi da kungiyoyin gwaji tare da kungiyar sarrafawa.
'Yan Adam sun samar da Iphayan da ke ware daga jinin mutane ta hanyar gradient centrient centrifugation. Waɗannan sel ne sel guda na nucleus musamman da aka haɗa da lymphocytes (t sel sel, da sel sel, da sel sels) da muracytes.
Bayanin samfurin:
|
Suna |
Abu ba |
Gwadawa |
Matsayin sel |
Adana / Jirgin ruwa |
|
Mononukister na mutum |
082A01.11 |
5 miliyan sel / ml |
Ɗanye |
2 - 8˚c ajiya, kankara shirya kaya |
Aikace-aikacen samfurin:
A - Binciken Metabolism na kwayoyi.
