Aikace-aikacen Cytolom P450 (Cyp450) da UDP - Glucuronossltrasansfres (Ubt) Enzymes a cikin Magunguna na Muryungiyoyin ƙwayoyi game da cututtukan fata na metabolic da enzyme

Kalmomi masu mahimmanci: Miyagun ƙwayoyi (DDI), Cytochrrove P450 (Cyp450 enzuronossyltracing, Cyp4CP9, CypzeNa2, CyP2C19, Cyp2C19, Cyp2d6, Cyp3a4 / 5, Cyp2a6, Ugt1a1, Ugt1A4, Ugt1A4, Ugt1A9, Ugt1A9, Ugt2B15, Ugt2B17, Ugt2B17, Ugt2B17.

• Samar da samarwa

SAURARA: Mafi yawan enzymes ana amfani dasu daNadph regeneration/ NazphdaPls
Yawancin enzymes yawanci ana amfani dasu tare daTsarin tsari na UGTda kuma pbs.

Metabolism yana taka muhimmiyar rawa a cikin makomar magunguna, yana shafar haɗarinsu a jiki kuma don haka yana haifar da manufa da tasiri. Wannan ya faru ne a hanta, amma yana iya faruwa ne a cikin gabobin gabobin. Karatun kwanan nan ya bayyana wanzuwar da mahimmancin metabolie enzymes (dme) a cikin kwakwalwa.P450 enzyme (cyp)daUDP - glucuronosyltrasan) Hakanan suna kan mahalarta mahalarta kan ƙwayoyin cuta kan kwayoyi a cikin tsarin juyayi na tsakiya (CNS).

• Cytochrome P450 (Cyp)

Cytochromer P450 na mamaye lokaci na metabolism (oxidation, raguwa, hydrolysis), lissafin hydrolysis), lissafin sama da 75% na metabolism miyagun ƙwayoyi. Subtypes MOSTypes sun haɗa da Cypa4 (50% miyagun ƙwayoyi masu magani) da cyp2d6 (20% miyagun ƙwayoyi). Cyp ya canza magungunan Lipophilic cikin Metabolites na Polar, inganta excroption.

Prime Hepatocytes ne hepatocytes ware kai tsaye daga hanta m mutum, kuma na zama samfurin da aka fi so don bincike mai zurfi na ƙwayar cuta saboda kiyaye ayyukan intic enzyme. Babban tsarin heckatocytses shine mafi yawan samfuran kimantawa na Cyp enzyme a masana'antu, inchymia, da hukumomin gudanarwa. A matsayinka na tsarin salula, kayan aikin ɗan adam, masu fafuti da kuma masu hana kwayoyi, da kuma masu gabatarwa.

• UDP - glucuronosyltrasan)

UDP - Glucuronosyltransferesltrasferferesltrasferferes shine farkon lokaci ⅱ enzurdoic acid ne mai ba da gudummawar sukari na glucuronic acid tare da abubuwa masu ban sha'awa da ƙungiyoyi masu ban sha'awa. An rarraba ɗanyuwar ɗan adam da aka bayyana a cikin kyallen takarda kamar hanta, ƙananan hanji, koda, ciki, da huhu. Haɗakarwar ita ce babban sashin jiki a cikin jikin mutum wanda ya ɗauki halayen glucuronic na glucuronic na glucuronic, kuma ana bayyana ƙananan subtypes a hanta. Ugt1a8, Ugt1A8, Ugt1A10, kuma Ugt2a1 an rarraba shi a cikin kyallen takarda da ke faruwa a cikin kyallen takarda da ke da alaƙa da sha da compretition magunguna.

• Aikace-aikacen maɓalli a cikin ci gaban magunguna

A cikin samfurin gwajin Vitro: Cyp enzymes kamar Microsocytes ana amfani da gwaje-gwaje na mitabanci / hepatocytes suna tabbatar da gwaji na metaboles (kamar kimanta gwaje-gwaje na 'yan takara (kamar su cyp3a4 hana gwaje-gwaje). Amfani da Gene da aka shirya don hango bambance-bambance na asibiti na asibiti.

Magunguna - hulɗa maciyin magani (DDI)Yi nazari: Gudanar da gwaje-gwajen Cyp don gano ko magunguna masu jan hankali suna hana hadarin Clinze. Gudanar da gwaje-gwajen hana yin gwaje-gwaje don kimanta tasirin kwayoyi akan ayyukan ƙawance. Gano tasirin shigar da kwayoyi akan cyp / Ugt ta hanyar bincike na farko na hepatocytes na bincike.

Haɓaka hanyoyin bincike na halittu: Nazarin Cyp da Enzymes na Sihiri da Ingantaccen Hanyar Magunguna da Magungunan Magunguna (DPm). Yi amfani da matrioles na halittu waɗanda ke ɗauke da cyp / Ugt metabolites (kamar bilama da plasma da aka taso kimanta kimantawa na matrix, kuma guje wa hanzari na ion / haɓakawa.

Binciken Polymorphiszarin bincike: Ugt1a1 da sauran halittar halittar UGT Enzyme magana ne mai mahimmanci da ke da kwayoyin halittar dan adam. Tare da ci gaban Pharmacogenomics, an gano cewa ilimin ilimin halittar jini yana da alaƙa da wasu matakan cututtukan metabolis, wanda bi da bi yana shafar abin da ya faru, ci gaba, da magani na cututtuka da sauran fannoni.

Yi nazari akan fannin bambance-bambance: Kwatanta bambance-bambance a cikin ayyukan enzyme na farko na hepatmyes na mutane, berayen, da kuma inganta dabarun canzawa zuwa amfani da asibiti zuwa amfani da asibiti.

• Hanji

Cyp Enzyme yayi kirahanjiYana nufin sabon abin da wasu mahadi zasu iya hana ayyukan wasu enabolesism na cyp450, sakamakon ya kara da cewa a hade, ta haka ana amfani da haɗarin aminci. Dangane da nau'ikan nau'ikan hanyoyin hana, hana tasirin magunguna a kan enzemes a cikin enzymes da lokaci - hana shi (TDI). Lokacin dogaro da lokaci, wanda aka sani da wanda ba a bayyana shi ba da izinin shiga, gaba ɗaya yana nufin samuwar wani hadaddun 'yan takara da cyp enzzentse ta hanyar rashin daidaituwa. Tasirin tasirin inhibitory tasirin inhibitor a enzyme ba ya bace nan da nan bayan da baƙon an cire shi, amma yana nuna lokaci - halaye na uku.

• Cyp450 enzyme na yau da kullun

A halin yanzu, akwai manyan hanyoyi guda uku don gano enzyme na enzyme na cyp450: Hanyar sadarwar halittu, da hanyar sadarwar halittu, da hanyar bincike ta Isoenziti. Za'a iya raba hanyar hanawa cikin hanyar shayarwar sunadarai da hanyar enhibody. Ya ƙunshi auna aikinta na rayuwa na microsomes akan magunguna da kuma ba tare da ƙarin metaboles ba, kuma infer a cikin metabolic na ɗan adam phenotype. Daga gare su, hanyar hana kayan aikin sunadarai ana amfani dashi saboda sauki ta hanyar aiki da araha.

• Ƙarshe

Cyp enzymes da enzymes na Ugt Exzymes, kamar yadda Core enzyme tsarin metabolism na miyagun ƙwayoyi na (oxidnation, raguwa) da na yau da kullun (glucicity, da kuma yawan amfani da kwayoyi. Subtypes kamar cyp3a4 da cyp2d6, tare da enzymes kamar urt1a1 da Ugt2b7, samar da hanyar sadarwa ta rayuwa. Abubuwan da suke banbanci na aikinsu (kamar su polymorphism da kuma nau'in takamaiman na farko) za a iya yin nazari ta hanyar ƙirar hepatocytes da LCTocytses, samar da tallafin bayanan da ke tallafawa don haɓaka magunguna.

Takardar shaida

Zhang, M., Rottschäfer, V., & cm de Long, E. (2024). Babban tasirin cyp da miyagun ƙwayoyi - enzzoling enzymes akan kwakwalwar kwayoyi da aka yi niyya. Magungunan miyagun ƙwayoyi, 56(1), 1 - 30.

Ghosal, A., Ramathan, R., Kishnani, N. S., Chowdhury, S. K., & alton, K. B. (2005). Cytochrome P450 (Cyp) da UDP - GlucuronosyltrasansfresmransfessmormTransflesm (ULT) Enzolmesm, Matsayi a Metabolism Magunguna, Polymorphism, da kuma gano yadda aka sa hannu a metabolism magani. A Ci gaba a cikin binciken magunguna da bincike na biomedical (Kundi 6, PP. 295 - 336). Eldvier.