CGTirƙirar Magana ta CGT - Lentivirus - CAR CARKONE: T

CGTirƙirar Magana ta CGT - Lentivirus - CAR CARKONE: T

Ingantaccen tantanin jikin mutum shine ingantaccen magani na cutar kansa wanda ke inganta tsarin rigakafi na jiki don niyya da cire sel na ciwon daji. Siffofin Mabrs sun hada da Cytokine - Haƙurin Killaral Cellipy, T - Cell Peal, da Motsa - T Tsarin Kwallan. Mota - T arhapy ya shafi taƙaitaccen t sel don bayyana chigen antigen antigory na musamman na cutar sankara kamar leckemiya da lymphoma.

Keywords: Keywords: Car - T, Lentiviral, Vector Lentiviry, Tracer Lentivirus

Shiri na mota - T Cel Peal

Gabaɗaya, mabuɗin manyan matakai don shirya motoci - t sel sel kamar haka:

Cibiyar FILT: Dana Takatarwar Cibiyar Farko

1. 1.Soring (t - kadaici)

Mataki na farko a cikin shirya motoci - t sel sel zai ware t - sel daga farin sel a cikin jinin mai haƙuri. Hanyoyi kamar dabaru na Bead ko kuma ana amfani da Cytometoret ko kwarara Cytometory ko kuma ana amfani da su don cire CD3 + - Rich T - sel daga jini. Don tabbatar da tsarkakakkiyar tantanin halitta, tsari na rarrabewa yana buƙatar ingantaccen tsari, tabbatar da cewa an samo sel mai girma - ingancin t - an samo sel.

2. 2.timulation (t - Kunnen tantanin halitta)

An fitar da sel da aka kunna a cikin vitro, yawanci amfani da aCD3 / CD28 T - Kwayoyin kunnawa / Texan Kit. Kwayoyint na Terys suna shigar da yanayin aiki, shirya su don samun damar samar da kayan. Ta hanyar wannan tsari, Talkirar tantanin talla yana inganta, wanda ya ba da gudummawa ga gyaran kwayar halitta da amplification.

3. 3.Transness (gyaran ganyen)

Sellar da aka kunna Tashar Tashar Tasirin Gasala don bayyana chigen Antigor Antigor (mota). Wannan tsari yawanci yana amfani da shiVector Leentcordon gabatar da kwayoyin halittar cikin sel. Lentivirus kayan aikin transdugaction na tantancewa na tantancewa, tare da fa'idar da ke tattare da gemen kwayar halitta (kamar gene gene) a cikin kwayar halitta. Ta hanyar hade da sel sel da kuma cin zarafin motar motar a cikin Cell, Lentivirus yana kunna sel don bayyana abubuwan da suka yi. Gashin mota ya hada da guda - sarkar sarkar (SCFV) na otibody wanda ke ba da takamaiman sel sel sel. Kamar yadda aka nuna a cikin Hoto na 2. Tasirin Lentvory yana da halayen ingancin da kwanciyar hankali, don a iya bayyana Gene Gene a cikin sel na dogon lokaci.

Figure: Motar Singapore - T Cely Terrapy

4. 4. blogplification (yaduwar sel)

Bayan cin nasara, mota - T sells Shigar da matakin amplifiation, inda dalilai girma kamarIl - 2an kara su don inganta yaduwar sel. Dalilin wannan tsari shine tabbatar da cewa yawan adadin motar - suna samuwa don jiko na jiki a cikin mai haƙuri. Dole ne a gudanar da ingantaccen tsari a ƙarƙashin yanayin bakararre don garantin ƙimar tantanin halitta da yawa.

5. 5.qalicticle iko (qc)
6. Kafin karuwa cikin haƙuri, kulawa mai inganci tana da mahimmanci a lokacin shirin. Car da Cars - Tells Seltro Stort TestTabbatar da tsarkakakkiyar su, aiki, ingancin kamuwa, da kuma bin ka'idodin magani. Bugu da ƙari, gwaji don gurɓataccen sel ko halayen ɓoyewa wajibi ne don tabbatar da amincin da maganin warkewa bayan ƙwayoyin cuta bayan haɓakar ƙwayoyin cuta bayan haɓakar ƙwayoyin ido bayan sun inganta shi.

IYanayin Lensivirus yana canzawa cikin sel na rigakafi

Fasaha ta Lentviy-kebura tana da mahimmanci a cikin maganin ƙwayoyin rigakafi, musamman ga gyaran ƙwayar da injiniya na sel. Vectors na LentShiry, sun samo asali ne daga ajiyayyen HIV - 1, ana amfani da su saboda ikon yin hadar da kwayoyin halittar cikin rundunar, tabbatar da faɗar lokaci. Zasu iya harba duka rarrabuwa kuma ba - rarraba sel, da amincinsu ya inganta ta hanyar gyara kayan kwastomomi don hana yin hankali. Yanzu haka ana amfani da waɗannan vectors a cikin maganin Gene, gene gene, da kuma bincike na ilimin tantanin halitta don ingancinsu da ƙawance a cikin isar da ilimin.

Faidai: Obiosh.com/production/pproduct

1. Isasshen Gyarar Gaggawa

Vector Levent Vector na iya isar da kwayoyin halittar, kamar galitar na mota, kamar yadda aka haɗa da waɗannan halittar jiki a cikin sel ana ci gaba da bayyana a lokacin sel. Wannan babban lambar - ingantaccen iko na dawowa shine babban abu ne ga nasarar rigakafi.

Omplower immunoenicity 

Idan aka kwatanta da sauran vector vectors, lenivires suna da ƙananan immunosenicity. A sakamakon haka, lokacin da ake amfani da lentiviruses don watsa sel na rigakafi, tsarin rigakafi na haƙuri yana haifar da mai rauni mai narkewa ga ƙwayar cuta. Wannan yana taimakawa wajen rage halayen mara kyau, musamman a cikin dogon - Rayuwar sel bayan jiko, game da jiko, game da jiko, hakan yana hana kin amincewa da yawan rigakafi.

3.Hayamuukakawa ga sel na rigakafi

Vector Leentcorctor Vector yana da ikon sarrafa nau'ikan ƙwayoyin rigakafi iri-iri,musamman t sel da sel. Ta hanyar jigilar Lentivaly, sel na rigakafi na iya samun ƙwayar cuta - Babban kwarewar fitarwa, ta haka haɓaka damar su don cin amanar da ƙwayoyin tumo.

4.Precion na sel mai tasiri a cikin wakiltar sel mai kyau 

A cikin ƙwaƙwalwar ajiya,sel trasorryKoma zuwa sel na rigakafi cewa, bayan transduction, Express Motsa da Magana ta Thie. Masu ba da lada na ci gaba da tantance kwayoyin a cikin wadannan sel mai tasiri, ba su damar gane da kuma manufa ta ƙwayoyin cuta.Sel manufasune ƙwayoyin tumo waɗanda ake gano su kuma sun lalata su da waɗannan sel na rigakafi. Ta hanyar jigilar Lentivaly, sel na rigakafi na iya haɓaka takamaiman bayani game da kashe ƙwayoyin tumo, don haka inganta ingancin warkewa.

A cikin wannan mahallin, Iphayan, IShase, ISHE OF KEDO COTTUGGING DA MULKIN SAMU NASARA DA Inganta nasarar isar da ilimin ƙwaƙwalwa. Waɗannan samfuran suna ba da ɗaya - dakatar da bayani ga abokan ciniki.

LentivirusProducts

Kit ɗin Iphates Leentivirusya ƙunshi duk masu sanannun reagents da ake buƙata donKulawa da lentivirus, yana sauƙaƙe yin gwaji da lokaci da ƙoƙari. Wannan kit ɗin ya haɗa da:

• Fartajadaukakawar Plasmid Mix
• Maimaitawa
• pegfp plasmid
• Iphayan Leentivirus Predestation

Wannan kayan aikin da gaske yana da gwajin! Ba tare da buƙatar shirye-shiryen da rikitarwa ba, za'a iya kammala aikin saiti cikin lunivirus wanda ya cika shi ta hanyar bin umarnin da aka haɗa.Abubuwan da suka dace da fa'idodin kit ɗin sun haɗa da gajeren lokaci, mai kyau mai amfani da hoto, da kuma aiki mai sauki, yana sanya shi da kyau ga masu farawa a cikin kunshin Lentivirus. Kowane saiti na reagents sun sha karfin ingancin ingancin tabbatar da ingantaccen, ingantaccen sakamako, ta inganta tallafin fasaha na ko bidiyo, ta wajen samar da tallafin fasaha.

Bugu da ƙari, don taimakawa abokan ciniki su ci gaba da farashi, Iphayan kuma ya haɓaka kuma ya samar da ingantaccen ƙarfi, yana ba da ingantaccen bayani mai sauri.Ta hanyar hadawa da Lentivirus Surfnatant tare da maida hankali sake, bi da wani taƙaitaccen shiryawa da centrifugation ta amfani da daidaitattun centrifug da sauri. Wannan tsari baya buƙatar ɗimbin ɗaci, yana sa shi dacewa da farashi mai dacewa - Zabi mai tasiri don yawancin masu amfani da ɗakunan gwaje-gwaje.

A takaice, Iphayan Leentivirus Predentoration Readant ba kawai yana inganta Ingantarwa ta hanyar wucewa don samar da vector na yanar gizo da sauri don yin rigakafi. Abu ne mai mahimmanci don gwaje-gwaje na yau da kullun, bincike na ganowa, da sauran wuraren aikace-aikace.

A taƙaita, komoniya. Ta hanyar aiwatar da matakai kamar sel rarrabe, kunnawa, transduction kayayyakin, masana kimiya na iya shirya wa marasa lafiya magani, suna ba da izinin maganin cutar sankarau.Tasirin Lentvalet, a matsayin fasahar Core, tana taka muhimmiyar rawa a cikin CARKO - T da mota - sel - sel - sel - sel - sel - sel - sel - sel - sel - sel - sel - sel - sel - sel - sel -Tare da ci gaba da cigaban fasaha, hana haifuwa ta ba da damar bayar da sabbin hanyoyin magani don ƙarin marasa lafiya, samar da darajar asibiti. A nan gaba, ana sa ran hana rigakafi zai kawo fatan juyin halitta don karin cutar da cutar kansa.

Don haduwa da bukatun tantanin halitta, magana, da bincike na cuta, iphayan da ke ba da kayan tenits, lenphirus maida hankali kan karagar mulki. Wadannan binciken lentiviruse suna daɗaɗa tare da furotin furotin mai haske (GFP), ja mai kyalli furotin prelling na sel duka a cikin vitro da kuma vivo.

Bayan haka, iphayan ma na iya yin pre biyu pre - Puffed tracer lentiviruses: GFP & Luciflease da Marker & Luciflease. Ana iya zaba waɗannan masu harkar da aka haɗa ko haɗe dangane da bukatun gwaji da haɓaka inganci don Real - Binciken ƙwayoyin cuta.

IphayanImamiotherapyRmProducts

Baya ga lentivirus - samfurori masu alaƙa da aka yi amfani da su a cikin mota - T ko mota - NK magunguna, Iphayan, a matsayin jagora masu goyan baya a cikin CGR (sel da sauran filayen. Iphotes ya ci gaba da kuma samar da Leiyocytes da PBMCS (sel Mononukplear Sells) sun ware ta hanyar lethiphy, don tallafawa saurin ci gaban rigakafin rigakafi na rigakafin rigakafi na rigakafi na rashin rigakafi.

Kayan samfuran Iphayan suna bayar da waɗannan fa'idodi, kumaTakaddun shaida na bincike (CoA)Tabbatarwa daga Iphayan yana samuwa ga kowane tsari.

Aminci 

An fice samfuran Iphayan daga masu ba da gudummawa / dabbobin da suka yi gaba ɗaya Precops preccaukaka preccome preccome precs (hiv - 1/2, HBV, HCV, Syphilis).

Yarda  

Iphayan yana ba da sanarwar yarda da masu ba da gudummawa da aka sa hannu, tare da bayyanannu tabbatacce tabbaci.

Gwaninta

Abubuwan da Iphayanawa sun bayar, an samu ta hanyar Leukapheres, ƙwararrun ƙwararrun ƙwararrun suna kulawa da su. Kowane tsari yana tare da coa mai dacewa, kuma ana ɗaukar samfuran a ƙarƙashin sarkar sanyi don tabbatar da aminci, isarwa mai sauri, da tabbacin inganci.

Sabis ɗin Abokin Ciniki

Iphayan suna ba da daraja - inganci bayan - Sabis na tallace-tallace, tabbatar da sananniyar ƙwarewa da kuma ba da daɗewa ba.