Crossences na giciye na bincike na ruwa mai bincike

1 kayayyakin kayayyaki

2 Ayyukan ayyukan ilimin halitta 2 na cerbrosoninal ruwa: bioanalysis na kwayoyi kwakwalwa

Kwakwalwa ya kasu kashi hudu, gami da Sennenpholon, dan lishash, cerebell, da kuma ƙwaƙwalwar ƙwaƙwalwar ƙwaƙwalwar ajiya. Ana kiran cavities a sassa daban-daban na kwakwalwa, waɗanda ke cike daCerebrospinal ruwa (CSF). Cerebrospinal ne ya shiga cikin rarraba da metabololm na kwayoyi a cikin kwakwalwa da tsarin juyayi (CNS).

Tsarin Pharmatodnamics na karancin sha na sha magani, rarraba, metabolism na bincike, tare da mai da hankali kan sa ido kan magani na jini. Jinin kwakwalwar kwakwalwa (BBB) ​​mai canjin canji ne tare da zaɓin abubuwa masu wahala, wanda ke iyakance rarraba abubuwa masu cutarwa, wanda ke iyakance rarraba abubuwa masu cutarwa daga kwakwalwa yayin da ke hana su kwakwalwa. Lokacin da BBB ya canza matsayin magunguna, gano abubuwan da jini mawuyacin ra'ayi na iya nuna daidai yana nuna maida hankali ne a cikin kwakwalwa. Kawai amfani da bugun jini na jini a matsayin mai nuna alama don maida hankali a cikin dabbobi na ƙarshe na iya haifar da rashin daidaituwa na warkewa wanda ba chan magunguna da ba shi da izini. Sabili da haka, a matakin Macro, kwakwalwar nama Homogenization ko kwakwalwa na girke-girke na girke-girke ana amfani dashi musamman ga binciken magunguna a cikin kwakwalwa.

3 Muhimmin nazarin halittar ruwa a cikin ci gaban magunguna

Abubuwan da ke cikin furotin na CSF sun yi rauni sosai, kuma ana amfani da maida hankali ga magunguna a matsayin mai nuna alama a maimakon CNS free magunguna. Koyaya, aikin CSF wurare da yawa yana da hankali sosai fiye da na hanzari na jini, wanda ya haifar da isasshen haɗuwa da abubuwan da ke ciki na dabba. Ya danganta da wuri samfurin / lokaci da kuma hanyar gudanarwa, maida hankali akai-akai a CSF na iya bambanta sosai.Wucin gadi na zamani (wucin gadi csf, ACSF) shine mafita wanda ke kwaikwayon abin da ke ciki da aikin ruwa na halitta, kuma iya kimantawa CNS pharmakiretic bayanai a ciki a cikin vitro adme gwaje-gwajen. Bugu da kari, ana iya amfani da ruwa na wucin gadi don kimanta tasirin sa game da cutar kwakwalwa da takamaiman sunadarai.

4 Kalubalen bincike na bincike na zamani da wajibi na wucin gadi matrix

4.1 LITTAFIN / Clinical / Clinical LICH don samun ruwa na asali

FASAHA A CIKIN SAUKI CIKIN SAUKI CIKIN SAUKI. Yawan mutane masu lafiya suna buƙatar samun ruwa na fure ta hanyar lumbar huɗa a cikin lumbar huda ko malalen manoma, wanda ke haifar da haɗarin hanyoyin ba da gudummawa kuma yana da wuya a ɗauki masu amfani da ƙoshin lafiya. Ta amfani da wucin gadi na zamani /Simulated Cerbrospinal ruwa(wucin gadi csf /simulated csf)) matrix maimakon samfurori na asali don ingancin hanyar, guje wa batutuwa na yau da kullun yayin da yake adana matrix da kuma tsarin matrix.

4.2 matrix sakamakon haifar da mahimmancin bambance-bambancen mutum

Dalilin / mutum bambance-bambance a cikin abubuwan gina jiki (15 - 100 mg / dl), sakamakon sakamako a cikin LC - Ms / MS / MS / MS), musamman da aka bincika ingancin ƙwayar cuta. Ta hanyar tsara kayan gargajiya na wucin gadi / ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta / LIPID na fursunoni daban-daban, LC - ana amfani da fasahar MS / MS / MS / MS / MS / MS tana amfani da ƙayyadaddun ƙimar galibin CADVE da rage bambance-bambance na Inter Batch. Hakanan yana yiwuwa a daidaita matakin hannu (kamar cromprography na Hilic) ko haɓaka lokacin riƙewa don raba abubuwan da aka zina daga matrix.

4.3 Tasirin canje-canje a cikin abubuwan matrix na halitta akan bincike a kan yanayin cuta

Cutar Cestarspinal ruwa na iya ƙunsar abubuwan da ba shi da kyau (kamar raguwar kayayyaki na kwakwalwa (kamar hemoglobin ba tare da halayyar ionization (Il - 6), wanda ke tattare da halaye na LC - ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms / ms

5 giciye nau'in sabo

5.1 Farin Cowerpinalis(Ɗan adam csf)

Tsarin dabbobi na mutumShin Cerebrospinal Standard Standary ruwa na zinari, amma iyakoki na ɗabi'a suna haifar da ƙarancin samfuran halitta; Lowerarancin furotin (15 - 45 MG / DL), ƙarancin abun ciki, na buƙatar babban lambar sannu-- Ms / ms / ms / ms gano alamun alamun alama (kamar Aβ, Tau).

5.2 Cynomolus biri birerkey ceribrospinal ruwa (Biri csf / nhp csf)

Cynomakus Monkey na bireruShin ba shi bane - Model na Model na ɗan adam kusa da mutane, tare da ƙananan bambance-bambance na mutum, ya dace da kimantawa PK / Pd da kimantawa na PDmagunguna na al'ada, kuma shine mafi kyawun ƙirar ƙira don ɗaukar matakan binciken ƙwaƙwalwar ƙwaƙwalwar ƙwaƙwalwar ajiya mafi yawancin CNS.

5.3 Resus biri ta haifa da ruwa mai zurfi (biri csf / nhp csf)

Resus biri biriAn kwatanta da halayen cynomolus, yana da halayen amsar da ke da ƙarfi neuro kuma ya fi dacewa da cututtukan da suka shafi neurdoflammatory ko cututtukan ƙwaƙwalwar ƙwaƙwalwa ko magungunan ƙwaƙwalwar ƙwaƙwalwa.

5.4 Beagle Dokberpinal ruwa (Dog Karaya CSF)

Beagle cerberpinal ruwababban tsari ne na haƙuri na babban kariya, wanda ya dace da jini - Shafan Braurin kwakwalwa da kuma nazarin macromelecular macromelecular magani.

5.5 SD sd Cerebrospinal ruwa (Bera csf)

SD Baterpinal ruwaKasan kananan girma na Ceribrospinal ruwa, tare da daidaitawa mai dacewa - Maɗaukaki yana amfani da sigar kwayar halitta, wanda aka yi amfani da shi a cikin binciken cututtukan cututtukan neurdoGelsassion.

5.6 linzamin kwamfuta mai zurfi (Linzamin kwamfuta csf)

Linzamin kwamfuta na consbrobrospinal ruwaBabban samfurin gwaji ne a cikin bincike na Neuro, musamman amfani da shi a cikin samfurin cututtukan cututtukan cututtukan cuta (kamar cutar Alzheimer, CNS) da tsarin juyayi.

5.7 minpig ceribrospinal ruwa (Minipig csf)

Minipig na Cerbrosoninan ruwaTsarin Anatical yana ba da damar maimaita samfuri, sanya shi dace da tsawo - na zamani karatu. Koyaya, abun da abun da ke tattare da phospholipids ya bambanta sosai da na ɗan adam, kuma zaɓi na hanyar buƙatar tabbatar da shi.

5.8 zomo na zomo (Zomo csf)

Zomo csf Shin ɗayan nau'ikan gwaji ne da aka saba amfani da shi a cikin bincike na Neuro na Neuro, musamman a cikin cututtukan opphthmic, da jini) nazarin kwakwalwa, inda yake da fa'idodi na musamman.

Ƙarshe

Binciken nazarin halittu (CSF) yana taka rawar gani a tsarin mungiyoyi na tsakiya (CNS) da ke haifar da cututtukan fata (BBB) ​​da tsarin kula da cuta a cikin tsarin juyayi na tsakiya. Koyaya, yin amfani da CSF na halitta yana fuskantar manyan matsaloli, gami da daidaitattun halaye a cikin tarin bayanai, da kuma canza bayanan biochemical a cikin jihohin cuta. Dukkanin iyakokin ba da cikakken mahimmancin matrifes, wanda ke ba da tabbataccen daidaitattun abubuwa masu mahimmanci yayin da ake buƙatar ƙayyadaddun ayyukan bioanytical yayin da ake yin amfani da ɗabi'a da fasaha.

LC - Ms / MS ta fito a matsayin kayan aikin bincike na karatun CSF, yana ba da takamaiman magunguna da kuma dabarun magana da biomarers a ƙananan taro. Duk da haka, abubuwan da ke haifar da rikice-rikice ta hanyar fursunoni, Lipid, da abun ciki na lantarki a duk nau'in-bukatar haɓaka shiri mai hankali da yanayin chromatographal. A cikin matricifial CSF matripes, wanda aka dace da shi don kwaikwayon lafiya da yanayin cuta (E.G., an ɗaukaka shi cikin cutar ta Alzheimer kuma sauƙaƙe hadari.

Daga cikin samfuran dabbobi, Cynomakus Monkey CSF ta yi kama da CSF na ɗan adam kuma an fi son karatun CSF na Janar PD saboda bincike na neurnflammator saboda bayanin martaba na rigakafi. Model mai rodent (E.G., SD Berayen) suna ba da farashi - Babban - Zaɓuɓɓukan ƙira, yayin binciken Minipig, karatun minpig CSF ya ba da damar yin samfuri a cikin Long - Nazarin Magana.

A ƙarshe, haɗin nau'in halitta - takamammen matrices na zamani tare da ci gaba LC - Ms / MS ERDOROMOROMIES SUCTRESS m, ɗabi'a, da kuma bincike mai zurfi. Ci gaban ci gaba na gaba ya kamata a maida hankali kan zanen CSF da Inganta wuraren motsa jiki don mafi kyawun sakamako na asibiti.

Kalmomi masu mahimmanci: Cerebrospinal ruwa, wucin gadi csf, wanda aka saba, csf samfurin, na CSF), CSF), CSF), CSF), Cynomolus Samfuron ruwa (CSF), Resom), CSF), MS, CSF Samfuron, Resf), biri CSF, biri csf, biri CSF, beagle Dog CSFBrospinal ruwa, kariyar kare csf, sd csf, minpig csf, zomo csf, bincike na CSBRSP, bincike na CSB

Cite: Ban Wei - Kang, Yang Zhi - Hong. Gratso na bincike da Fasaha ta Fasaha ta Ci gaba a cikin Pharmacalingicsicainetics. Bulletin Maganar Prounderical, 2023, 39 (9): 1607 - 1612 - 16 - 1612 -