Menene PBMCs na ɗan adam da rawar da suke cikin rigakafi?

Dan Adam pbmcs, ko kuma kashi na mononukawar jini mononukbeledar, wata ƙungiya ce mai mahimmanci ce ta sel na rigakafi da ke rikon jini. Wadannan sel sun hada da lymphocytes, monocytes, da sel na Denditic, kowane yana wasa wani matsayi na musamman wajen kare jikinka. PBMCs tana kare ku ta hanyar ganowa, kamar ƙwayoyin cuta da ƙwayoyin cuta, yayin da kuma rike ma'aunin rigakafi.

Bincike yana nuna PBMCS sun kasance tsayayye a kan lokaci, tare da sikila ta sama sama da kashi 85% a cikin mafi yawan samfurori. Bugu da ƙari, magana ta gari za ta iya dacewa da sauri. Misali, magana tnfα tana ƙaruwa da sama da uku a cikin sa'o'i. Irin wannan ayoyi wanda ya nuna mahimmancinsu wajen daidaita amsoshi.

Abun da ke ciki na dan Adam

Lymphocytes: Tells Tel, BLEls, da Kwayoyin NK

Lymphocytes wani abu ne mai mahimmanci na pBMCs ɗan adam. Sun haɗa da sel, sells B, da kuma Killace Killace (NK) sel na halitta (NK) sel na halitta, kowannensu da mahimman matsayi a cikin rigakafi. Tellan sel suna taimakawa tsara amsoshin rigakafi da kai tsaye kai hari cutar sel. B sel sel suna samar da abubuwan rigakafi, wanda ke daidaita cutarwa na cutarwa. Kwayoyin NK, a gefe guda, manufa da lalata sel mara kyau, kamar waɗanda ƙwayoyin cuta ko ƙwayoyin cuta.

Abin sha'awa, wasu cymphocytes a cikin dan adam pbmcs, gano azaman sel cd3 + CD19 + sel, aikin Dual. Waɗannan ƙwayoyin za su iya yin amfani kamar sel da sel da B sel. Suna amsa barazanar ta t - Mai karɓar salon sel (TCR) da B - Mai karɓar sel (BCR) Siffallon Samfuran Samfuran. Wannan rawar na Dual yana ba su damar shiga cikin kuturta da kuma amsawar rigakafi. Misali, sun yi makoki antigens mafi inganci fiye da sel na al'ada kuma suna samar da Interferon - Gamma (I IFN - γ) a matakan kama da tls.

Monocytes da abubuwan da suka shafi su

Monocytes wani rukuni ne a cikin PBMCs na ɗan adam. Wadannan sel sintiri mai sinadarai, suna neman alamun kamuwa da cuta ko lalacewar nama. Da zarar sun gano matsala, sun yi ƙaura zuwa yankin da abin ya shafa kuma sun canza cikin Macrophage ko ƙwayoyin cuta. Macrophages enulf kuma narkewar ƙwayoyin cuta, yayin da sel sel suka fara antigens zuwa wasu sel na rigakafi.

Monocytes ma sakin Cyttones, waɗanda suke siginar kwayoyin da ke taimakawa daidaita amsar rigakafi. Ta yin hakan, suna tabbatar da cewa jikinka ya yi amfani da cututtukan da kyau ko raunin da ya faru.

Sel dedditic da rawar da su a cikin gabatarwar Athigen

Kwayoyin cuta na Denditic sune ƙwararrun ƙwararraki - Gabatarwa (APCs) a cikin PBMCs na ɗan adam. Suna taka muhimmiyar rawa wajen kunna sel ta hanyar gabatar da antigens a farfajiya. Bincike ya nuna cewa sel dedditic sune kawai aps kawai aps iya kunna duka CD4 + da CD8 + MVE TLEL. Abubuwan da suka dace ya zo daga ikon yin jinkirin narkewa na Antigen, wanda ke ƙara kasancewar abinci mai narkewa don loda na MHC.

Wadannan sel suna tabbatar da cewa tsarin garkuwar ku ya gane da kuma amsa barazanar da kyau, yana sa su zama dole a cikin rigakafi.

Ware na ɗan adam pbmcs

Sources na PBMCS: Jiki na Siferal da Marakashi

Za'a iya ware PBMCs na ɗan adam daga manyan abubuwan farko: jini da bargo na ƙashi. Jinin jini shine mafi tushe na gama gari saboda samun dama da ƙarancin rai. Bararo, a gefe guda, yana samar da yanayin richer don sel na rigakafi amma yana buƙatar tsari mai amfani.

Yawan amfanin ƙasa da tsabta na PBMCs na iya bambanta dangane da tushen da hanyar ware. Misali, nazarin nuna cewa daidaitaccen tsari na ƙasa yana samun babban yawan amfanin ƙasa da tsarkakakke idan aka kwatanta da cpt (tsarin shiri na tebur). Teburin da ke ƙasa yana ba da haske game da waɗannan bambance-bambance:

FICOLL PRALLY dabara don PBMC Kadaici

Ficoll Ficoll Pricanlay shine hanyar da aka yi amfani da ita don isolating pbmcs. Wannan tsari ya shafi zama ruwan sama akan FICOLL - mafita paque da centraiching shi don rarrabe sel dangane da yawa. PBMCS samar da takamaiman Layer tsakanin plasma da fikafar, yana sa su tattarawa.

Bincike yana ƙarfafa mahimmancin aiwatarwa yayin wannan aikin don tabbatar da sakamako mai mahimmanci. Misali, binciken guda ɗaya da aka samo cewa amfani da ficoll daidai zai iya cimma tsarkin sama zuwa kashi 97% tare da ƙarancin canji. Teburin da ke ƙasa ya kwatanta tsarin da ke tattare da keɓaɓɓun hanyoyi don pBMC kadaici:

Hanyar ware ta Imstunomagnic

Rashin daidaituwa na Imstunomagnic shine wata dabara mai ci gaba don ware pbmcs. Wannan hanyar tana amfani da beadsan wasan magnetic mai rufi tare da ƙwayoyin cuta don neman takamaiman nau'in kwayar halitta. Kyakkyawan rarrafe sel ne ta hanyar ɗaure su zuwa beads, yayin da sutturar mara kyau tana cire ƙwayoyin da ba'a so ba, suka bar jama'ar da ake so waɗanda ake so.

Bincike ya nuna cewa rashin daidaitawa yana kula da yanayin tantancewa kuma baya shafar alamomi kamar Il - 2r (CD25). Sabanin haka, ingantacciyar rarrabawa na iya rage ƙarfin hali da kuma taka tsantsan, musamman bayan karfafawa. Tebur da ke ƙasa yana taƙaita waɗannan binciken:

Wannan hanyar tana da amfani musamman lokacin da kuke buƙatar takamaiman ƙirar kwayar halitta don bincike ko aikace-aikacen warkewa. Aikace-aikace na warkewa.

Aikace-aikacen ɗan adam a cikin bincike da magani

Matsayi a CAR - Batun Taro

PBMCs na mutane suna taka rawa a cikin motar da ke ciyar da mota - T Cel Palt. Wadannan sel suna yin aiki a matsayin kayan fara don samar da sel - a cikin sel. Kuna iya mamakin yadda ingancin PBMCs suke cikin wannan tsari. Nazarin ya bayyana sakamakon ban sha'awa:

• Bayan kwanaki 11 na al'ada, 1 × 10 ^ 7 daskararre pbmcs na iya samar da aƙalla 1.48 × 10 ^ 9 Mesocar Sells 30% na Car + sel.

• Gwajin Cytotoxicity Nuna Mosicar - sels sel da aka samo daga sabo da kuka mai yawa pbmcs yi daidai. A wani sakamako - zuwa - Tarayyar Turai na 4: 1, Lungunsu na Cytotoxancity tsakanin 91.02% - 95.46%, bi da bi.

• Ko da a ƙaramin rabo na 2: 1, babu wani muhimmin bambanci a cikin cytotoxity an lura.

Waɗannan binciken suna ba da haske game da amincin PBMCS wajen samar da mota mai tasiri ko kuma bayan dogon lambar.

Yi amfani da gwajin magunguna da nazarin maye

Pbmcs ne m a cikin gwajin magunguna da kuma nazarin guba. Suna ba da ɗan adam - Model mai dacewa don kimanta yadda kwayoyi suke hulɗa da ƙwayoyin rigakafi. Misali, masu bincike sun gwada cutar quinacrine a kan PBMCs don tantance guba. Tebur da ke ƙasa yana taƙaita binciken:

Waɗannan sakamakon suna nuna cewa PBMCS sun amsa da kyau ga Quinacrine, har ma da ƙananan matakan isa. Wannan yana sa su ingantaccen kayan aiki don tsinkayar amsawa ga sabbin magunguna.

Gano BIOMARKER DA KYAUTA

PBMCS suna da mahimmanci don gano halittu da saka idanu lafiya. Biomarkers sune alamun alamomi na tsarin halitta ko cututtuka. Ta hanyar nazarin pbmcs, zaku iya fallasa fannonin halittar da ke bayyana ayyukan rigakafi, ci gaba, ko tasiri ko da tasiri. Misali, masu bincike sun auna matakan Cytokine a cikin PBMCs don saka idanu a kan amsar rigakafi a lokacin cututtukan ko kwantar da hankali. Wannan hanyar tana taimaka wa jiyya ga marasa lafiyar mutum, inganta sakamako.

PBMCs kuma yana ba da dogon lambar rigakafi. Zamanta da daidaitawa suna sa su zama masu dacewa don canje-canje na bin dama a cikin aikin rigakafi a lokaci. Wannan yana da amfani musamman a cikin cututtuka na kullum ko a lokacin proloned jiyya.

PBMCs 'yan Adam ba makawa ne a fahimta da kuma ciyar da kwayar cuta. Abubuwan da suke ciki da ke ciki-lymphocytes, monocytes, da sel sel-yana ba su damar yin mahimmancin ayyuka na rigakafi. Abubuwan da ke motsa jiki kamar FICORTNETICE na FICUNCOLLE na tabbatar da cewa zaku iya samun babban - tsarkakakken pbmcs don bincike ko amfani da warkewa.

Aikace-aikacen su sun cika filayen da yawa:

• Dubun dubatar karatu sun yi amfani da PBMCs a cikin binciken asibiti a cikin shekaru 50 da suka gabata.

• Suna da mahimmanci don mota - T Terar Celt, Ci gaban ƙwayar cuta, da kuma tantanin martani na rigakafi.

• PBMCs suna ba da gudummawa ga gano BIOMARKER, da rashin haƙuri.

Ta hanyar leverarging pbmcs, zaku iya buše sabon damar a cikin ilimin rigakafi da haɓaka ingantattun hanyoyin da suka canza lafiya.

Faq

Menene pbmcs da aka yi amfani da su a cikin binciken likita?

PBMCS suna taimakawa masu bincike na binciken martani na rigakafi, gwada sabbin magunguna, da kuma haɓaka magunguna kamar mota - T Tellments. Abubuwan da suka dace da kwanciyar hankali suna sanya su dace don gwaje-gwajen da ke buƙatar sel na rigakafi na mutum na rigakafi.

Taya zaka adana PBMCs don amfanin nan gaba?

Kuna iya adana pbmcs ta hanyar cringopreserpinsu a cikin nitrogen ruwa. Wannan hanyar ta tabbatar da kyakkyawar rayuwarsu da ayyuka, tana ba ku damar amfani da su cikin dogon-lokaci na zamani ko aikace-aikacen warkewa ko aikace-aikace na warkewa ko aikace-aikace na warkewa.

Shin pbmcs iri ɗaya ne kamar farin sel?

Ba daidai bane. PBMCs sune sel sel sel da sun hada da lymphococytes, monocytes, da sel na Denditic. Sun ware Granulhocytes kamar neutrophils, wanda kuma shine rabin farin sel.

Za a iya amfani da pbmcs don nazarin cututtukan autoimmune?

Ee! Pbmcs suna da mahimmanci don yin nazarin cututtukan autoimmune. Suna taimaka muku tantance halartar rigakafi, samar da cututtukan Cytokine, da alamomin kwayoyin, da kuma ma'anar fahimta cikin hanyoyin cututtukan da jiyya.

Shin pbmc kadaici tsari mai rikitarwa?

Ba da gaske ba. Dabaru kamar hanyar ficlay ficlay ko kuma imminomagnetic rabuwa sanya pbmc kadaici madaidaiciya. Tare da ingantacciyar horo da kayan aiki, zaku iya samun babbar samfuri tsarkakakku don bincikenku.