Ci gaba a cikin bioanalysis: Matsayin LC - ms / ms a ophthalmic much ci gaban da ruwa-ruwa da kuma bincike mai-wumor

Kayayyakin iphayan

Liquolography - Tandem Mass Spectrometry (LC - MS / MS)

Liquidraphraphy - Tandem Mass Spectrometry (LC - ms / ms) Matsaka ne dabaru mai ma'ana wanda ke hada karfin rabuwawar ruwa na ruwa na tantance taro na Tandem taro. A cikin LC - MS / MS, samfurin cakuda an raba shi da ruwa Chromatography, inda aka raba saboda rabuwa yayin da suke wucewa ta shafi. Abubuwan da aka raba suna topized da kuma bincika ta Tandem taro na spectrometry, wanda gungument ions cikin yanayin samfurin don cikakken bincike na tsarin.

Aikace-aikace na LC - MS / MS A cikin Bioanalysis

Bioanalysisya ƙunshi ma'aunin maida hankali da magunguna, metabolites, da sauran mahadi na halittu a cikin samfurori na halitta, kamar jini, plasma, fitsari, da sauranbiofliyeIDs. LC - MS / MS tana da kyau sosai - sun dace da waɗannan aikace-aikacen saboda ikon gano ƙananan taro na maƙarƙashiya.

LC - MS / MS Fasaha don bincike game da samfurori na halittu na gano abubuwa masu ban tsoro da engenous. Masu binciken sun kwaikwayi samfuran gaske ta hanyar ƙara kayan da za a auna ablank matrixDon tsara samfurin adadi mai yawa da samfurin ingancin sarrafawa. A maida hankali ne na kayan da za a auna a cikin samfurin halittar halittu ana ƙididdige shi ta hanyar daidaitaccen tsari.

Abubuwa masu zuwa suna da abubuwa waɗanda ke faruwa ta halitta a jiki. Magungunan Elegenous - Magungunan da suka shafi sun zama wata hanya mai mahimmanci don sabon ci gaban magunguna a cikin 'yan shekarun nan. Tare da haihuwar yawancin adadin magunguna tare da abubuwa masu kyau, bioanalysis na kwayoyi tare da abubuwa masu kyau sun zama mafi mahimmanci. Koyaya, a halin yanzu, ingancin samfurin nazarin halittu Abubuwan Bincike na kayan tarihi ta hanyar FDA da kuma gabaɗaya, ingancin yanayi, ƙima mai gamsarwa. Tunda gano abubuwa masu kyau suna haifar da matsaloli a cikin sakamakon gano saboda tasirin sa yayin samun matrix ɗin sa yayin samun matrix na ainihi, fitowar madadinmaras amfani da matrix matrix (Abubuwan da ake amfani da matripifial) Yana warware wannan matsalar.

Tebur 1: Bayanin Zabi a cikin Babban Tsarin Masana'antu na Kasuwanci

Binciken Hanyar Inganta da Ingantaccen Hanyar Gaskiya

A cikin bioanalysis, tabbatar da amincin sakamako ne mai ma'ana. Wannan yana buƙatar tsauri daIngantaccen bincike na nazariHanyoyi.

Binciken Hanyarya ƙunshi ƙirƙirar hanyoyin inganta hanyoyin don ganowa da kuma tantance masu amfani. Wannan ya hada da zaɓin yanayin Chromatographic da ya dace (Elig., lokacin aiki, lokaci, yadudduka na ƙarfin) da kuma ms, ionizize dabarar) da kuma cinye mafi kyawun dabarar) don cimma kyakkyawan ji daɗi, ƙuduri, da kuma zavi. Ari ga haka, hanyar dole ne ta iya zama daidai takamaiman tsarin adadi a gaban matattarar halittu da kuma wasu mahimman abubuwa, da sauran mahadi waɗanda zasu iya tsoma baki game da bincike.

Da zarar an inganta hanya, dole ne ya shaTabbatar Hanyar Hanyar Gaskiyadon tabbatar da cewa ya cika ka'idojin aikin. Wannan tsarin ingancin ya zama dole don tabbatar da cewa hanyar ta dace da manufar da ta yi niyyar da kuma tazara tare da buƙatun mulfulory. Don hanyoyin yin abubuwa, ingantawa yawanci sun haɗa da yawancin sigogi masu yawa:

• - Daidaito da daidaito:Tabbatar da hanyar yana ba da madaidaiciyar sakamako.
• - SENEITHAVELA:Ikon gano ƙananan taro na mai bincike.
• - Zabi:Ikon hanya don bambance mai bincike daga sauran mahadi a cikin matrix.
• - Recover:Ingancin da abin da aka gabatar da masu bincike daga samfurin nazarin halittu.
• - Kwanciyar hankali:Dangi mai bincike a karkashin ajiya daban-daban da yanayin kulawa.
• - Layi:Ikon hanyar da za a samar da sakamakon da ke daidai gwargwado ga nazarin maida hankali kan kewayon da aka kayyade.

Matsayi na ɓoyayyiyar matrix da blank matrix taka Matskawa Matsayin A cikin wannan tsarin ingantawa. Wadannan samfurori masu sarrafawa, waɗanda ba sa ɗauke da nazarin sha'awa, suna da mahimmanci don gano yiwuwar matrix da ke haifar da tasirin matrix ko rudani yayin bincike. Suna taimakawa wajen tabbatar da matakan kwari don nazarin da tabbatar da cewa matrix da kanta ba ta ba da gudummawa ga gurbataccen sigari ko kuma cikin. Hakazalika, amfani daMagunguna - matrices kyautaYana da mahimmanci don tabbatar da cewa babu magunguna masu saura ko metabolites suna nan cikin samfurin da zasu iya yin amfani da sakamakon.

Bioanalysis na magunguna ophthalmics

Bangon ƙwallon ƙwallon ƙwallon ƙafa ya kasu kashi uku, Layer waje shine membrane fibrane; Membrane na tsakiya shine membrane, membrane membrane ko UVEA; Kuma na ciki membrane ne retina. An raba kwallon ido zuwa kashi biyu, cututtukan ciki da na baya-ƙasa na ido, an ɗaure su bayan ruwan tabarau.

Hoto na 1. Anatomy na mutum ido.

Babban dabarun da ke da hannu a cikin metabolism magani sun hada da:

• Mabuɗin masara- Babban rukunin yanar gizon don karin sha da ƙwayoyi na p450 (Cyp) enzymes wanda ke haɗuwa da fromtrugs.
• Conjunctiva- Arts a cikin magani - enzymes (enzymes enzymes (enzymes (misali, esereses da cyps), gudummawar da farko - sama metabolism kafin sha.
• Gidan talakawa- Iyakar aiki na rayuwa amma yana taka rawa a cikin rarraba magunguna da kuma share su.
• M- Yin allura na ciki na iya yin aiki kai tsaye akan retina kuma ku rage guba a cikin cirewa mai cirewa. Smallan ƙaramar kwayoyin magunguna sun narke da sauri, da manyan kwayoyin kwayoyin suna da rabiakuna suna da rabi - rayuwa. Canje-canje na ciki tare da shekaru suna shafar magunguna na magunguna.
• Sclera- Sclera ya fi karuwa ga manyan kwayoyin kwayoyin da kuma nassi da kwayoyi ta hanyar sclera ne yafi damun girman kwayoyin halitta ke cutar da su. Ciyarwar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta ta ba da damar kwayoyi don shigar da Choroid, amma tsari yana da rikitarwa. ScLalal Melanin da ke ɗaure ƙwayoyi kuma yana shafar sakinsa da tsawon lokacin aiki.
• Yankin ido- Tallafin kyallen maimaitawa yana da wadataccen gudummawar jini da kwayoyi za a iya kawar da su ta hanyar kewaya jiki ko nono. Chorinoidal Hyperperlemacle yana ba da magunguna don sauƙaƙe shigar da sararin samaniya, amma yana da wuya a ƙetare epitheliy na rethalymeum, wanda ke da damar inganci da haifar da asara. Melanina - Bind kwayoyi na iya tsawan tsawon lokacin aiki.

Aqueous Humor da Bitreous Holor

Dagidan talakawadawalakokisuna da mahimmanci ruwa mai mahimmanci waɗanda ke yin rawar da ke kula da matsin lamba na ciki, suna ba da abinci mai gina jiki, da kuma sauƙaƙa madaidaiciyar tsabta. The aqueous humor is the thin, clear, watery fluid that fills both the anterior and posterior chambers of the eye, containing ions, proteins, carbohydrates, and oxygen. Yawancin abin da ke cikin ruwa mai santsi sun samar da shi ta hanyar cinya, ya fice daga cikin ido a cikin kusurwar da aka kirkira ta hanyar Iris da Cornea. Wadannan ruwaye sun bambanta a cikin jinsuna, gami da mutane, birai, zomaye, da sauran na farko. Yawancin lokaci suna tattara tare da manyan masu girma dabam daga mutum na dabbobi ko wuraren waha.

Aqueous Humor a ko'ina cikin jinsuna

Humor na ɗan adam

DaHumor na ɗan adamA bayyane yake, abinci mai narkewa - Rics ruwa wanda ke kula da matsin lamba na ciki kuma yana goyan bayan ayyukan da ke cikin metabolic da ruwan tabarau. An samar da shi ta hanyar cinya kuma yana kwarara ta hanyar ɗakin karko kafin magaraye ta hanyar tafiye-tafiye na fata.

Monkey Coumor Humor

DaMonkey Coumor Humora hankali yayi kama da na mutane a cikin kayan aiki da kuzari. Bayar da kamanceceniya da ke tsakanin prassics da mutane,Ban dace da kai tsayeYana aiki a matsayin mahimmancin tunani don karatun ophthalmic.

Rabbit aqueous

Darabbit aqueousBambanci mahimmanci daga wannan na Prusates, musamman a cikin furotin tattarawa da kuma yawan juyawa. Ana amfani da zomaye a cikin binciken OCular, duk da cewa dole ne a la'akari da takamaiman bambancin takamaiman.

Humor Humor a ko'ina cikin jinsuna

Harror na ɗan adam

DaHarror na ɗan adamGell ne - Kamar abubuwa da aka haɗa da ruwa, Collagen, da acid hyaluronic acid. Yana kula da siffar ƙwayar ƙwayar cuta, ɗaukar girgiza, kuma yana haifar da jigilar kayan abinci mai gina jiki.

Birkkey Bitreous

DaBirkkey Bitreousraba wani irin wannan abun da ake yi wa mutum-mutumi na mutum, yinnon - m ɗan adam mModel mai mahimmanci don nazarin shekaru - masu alaƙa da mahalarta da kuma masu alaƙa.

Rabbit verreous

DaRabbit verreousAn tsara tsari, kasancewa da ƙarin ruwa - kamar kuma yana da ƙananan ƙananan collagen. Wadannan bambance-bambance suna tasiri yadda ake amfani da su na fata da magungunan magunguna.

Ci gaban wucin gadi da simulatedwar kwayoyi

Wucin gadi mai ruwa mai ruwa da ruwa mai ruwa

Harshen Wucin gadidawucin gadiAbubuwan da aka kirkira da aka kirkira don amfani da su a cikin aikin ophthalmic, isar da miyagun ƙwayoyi, da aikace-aikacen bincike. Wadannan ruwayen ruwa masu kwaikwayo na bitoci da kayan jiki na abokan aikinsu na halitta.

Simulated ruwa mai ruwa da kuma simulated vitreous

Sumulated mai ruwadaSimulated Vitreous HolorShin dakin gwaje-gwaje ne - shirya hanyoyin da ake amfani da su a cikin gwajin vitro da kuma tallacen jiki na acular. Suna sauƙaƙe karatun da ke sarrafawa ba tare da halayyar ɗabi'ar da ke tattare da dabba ko samfurori na mutane ba.

Ƙarshe

Yin amfani da ruwa na chromatography - Tandem Mulci Spectrometry (LC - Ms / MS) a cikin Bioanalysis yana wakiltar ci gaba da ƙididdigar mahimman fasahohin, gami da kwayoyi da metabolites a matrices na halittu. Halin hanya mai girman gaske, daidai, da kuma zaɓi sanya shi mai mahimmanci a cikin abubuwan da ke cikin banbanci da ƙwayoyin cuta na ophthalmic. Cikakken fahimta game da ƙwayar ƙwayar cuta da kuma rawar da ruwa kamar tazari da kuma masu sanyaya-ruwa sun nuna mahimmancin waɗannan kayan haɗin kan ƙwayoyin cuta. Haka kuma, ci gaban wucin gadi da simulatedwar ruwa na kwayar halitta na bincike yana yiwuwa yana yiwuwa yana da damar yayin da tabbatar da la'akari da la'akari da la'akari. Kamar yadda ingancin hanyar nazarin ya ci gaba da samo asali, yana tabbatar da aminci da kuma sake haihuwa da ake buƙata don aikace-aikacen asibiti, musamman a ophthalomology.

Keywords: LC - MS / MS, Blank Matrix, Magunguna kyauta, Ingantaccen Tsarin Humor, ba na Tsabtace mutum-mutumo, Monkey Commatus HADOW, Rabbit Brodreous Hummer, ba - Pretimate dan adam mai matukar damuwa,Humor Humor, wanda aka buga aimated mawor, wucin gadi ruwa-wucin gadi, wucin gadi dumama, wucin gadi.

Takardar shaida

Seyeypour, s. M., ragowar, Rezazadeh, G., & Ricken, T. (2023). Mayar da ilmin lissafi na amsar mai ƙarfi na mai amfani mai haɓakar haɓakar haɓakar haɓakar haɓakar haɓakar haɓakawa mai ɗorewa.Aunawa: Sensers, 30, 100936. Https://dii.org/10.1016/j.measen.2023.100936