Nā Kūlana Kiʻekiʻe no ka CD3+, CD4+, a me CD8+ T Cell Isolation

Hua'ōlelo: CD3 + T cell isolation, CD4 + T cell isolation, CD8 + T cell isolation, CD3 + T Cells Positive Selection, CD3 + T Cells Negative Isolation, CD4 + T Cells Positive Selection, CD4 + T Cells Negative Isolation, CD8 + T Cells Positive Selection, CD8 + T Cells Cells Negative, CD8 + T Cells Cells Negative. kaʻawale, kahe cytometry

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Hoʻolauna

I ka ulu wikiwiki ʻana o ka immunology a me ka cellular therapy, ʻo ka pololei o ka hoʻokaʻawale cell e hoʻoholo i ka kūleʻa o ka noiʻi o lalo. ʻO ka hoʻokaʻawale ʻana o ke kelepona T ke kū nei ma ke ʻano he kaʻina pōhaku kihi, e ʻae ana i nā ʻepekema e wehewehe i nā paʻakikī o ka ʻōnaehana pale me ka maopopo ʻole. Ma ka hoʻohana ʻana i nā ʻenehana holomua e like me ka hoʻokaʻawale cell magnetic a me ka hoʻokaʻawale cell immunomagnetic, hiki i nā mea noiʻi ke hōʻiliʻili maikaʻi i nā heluna kanaka e aʻo i nā mea āpau mai nā ala autoimmune a i ka maʻi immunotherapy. ʻO ka hilinaʻi o ka immunomagnetic cell separation a me ka magnetic cell separation protocols e hōʻoia i ka kiʻekiʻe-koʻikoʻi ʻikepili i hana ʻia mai kēlā me kēia laʻana.

ʻO nā kumu kumu o ka hoʻokaʻawale ʻana i nā cell magnet no ka noiʻi

Ke hilinaʻi nei nā laboratories o kēia manawa i ka hoʻokaʻawale ʻana o nā cell magnetic no ka hoʻokele ʻana i nā ʻano like ʻole i loaʻa i loko o nā cell mononuclear blood peripheral (PBMCs) a i ʻole nā ​​mea i hoʻokaʻawale ʻia. Hoʻohana kēia ʻenehana i ka pilina ma waena o nā antibodies monoclonal a me nā ʻāpana magnetic liʻiliʻi, e hoʻopaʻa pono i nā cell o ka hoihoi. ʻO ke kaʻina hana o ka hoʻokaʻawale ʻana i nā cell immunomagnetic e hōʻoia i ka hiki ke hoʻokaʻawale ʻia nā lymphocytes i manaʻo ʻia me ka wikiwiki kiʻekiʻe a me ke koʻikoʻi mechanical liʻiliʻi, e mālama ana i ka pono physiological o nā cell no nā hoʻokolohua ma hope.

ʻO nā ala hoʻolālā i ka CD3+ T Cell Isolation

Ma ke ʻano he pan-T cell marker, he mea nui ka CD3 no ka ʻike ʻana i ka heluna T lymphocyte holoʻokoʻa. I ka hana anacd3+ t ka hoʻokaʻawale ʻana, Pono nā mea noiʻi e koho ma waena o nā ʻano hana like ʻole e pili ana i kā lākou mau pahuhopu hoʻokolohua.Koho maikaʻi ʻo CD3+ Te pili ana i ka hoʻopaʻa pololei ʻana o nā peʻa magnetic i ka antigen CD3, e hāʻawi ana i kahi hua maʻemaʻe kiʻekiʻe i kūpono no nā noi kahi e hoʻopilikia ʻole ai ka bead i ke aʻo ʻana.

ʻO ka ʻokoʻa,CD3+ T Hoʻokaʻawale ʻole ʻiahāʻawi i nā pūnaewele "ʻaʻole i paʻi ʻia" ma ka hoʻopau ʻana i nā cell non-T āpau. ʻO kēia ala CD3+ T Cells Negative Isolation i makemake nui ʻia no nā haʻawina hana kahi e noho ai ka mea hoʻokipa T cell i kona ʻāina hānau. Ke koho nei i CD3+ T Cells Positive Selection no ka hoʻoulu ʻana a i ʻole cd3+ t cell isolation no ka wikiwiki, ʻo ka pololei o nā hopena ka mea nui.

Pono i ka CD4+ T Cell Isolation a me Subset Enrichment

He mea koʻikoʻi nā cell T Helper i ka hoʻonohonoho ʻana i nā pane kūlohelohe, hanacd4+ t ka hoʻokaʻawale ʻanahe koi pinepine i ka noiʻi lapaʻau. Ke hoʻohana neiCD4+ T Koho Ponoʻae i ka hopu wikiwiki ʻana i kēia mau cell, e hōʻea pinepine i ka maʻemaʻe ma luna o 95% i ka pae hoʻokahi. No nā kānaka ʻepekema i kālele ana i nā ala hōʻailona kelepona,CD4+ T Hoʻokaʻawale ʻole ʻiae mau ana i ke kulana gula.

ʻO ka ʻokoʻa,CD3+ T Hoʻokaʻawale ʻole ʻiahāʻawi i nā pūnaewele "ʻaʻole i paʻi ʻia" ma ka hoʻopau ʻana i nā cell non-T āpau. ʻO kēia ala CD3+ T Cells Negative Isolation i makemake nui ʻia no nā haʻawina hana kahi e noho ai ka mea hoʻokipa T cell i kona ʻāina hānau. Hoʻoikaika ʻia nā ʻano ʻelua e hana i nā laʻana i mākaukau no ka nānā ʻana i lalo, e like me ka ʻike ʻana i nā subsets T cell ekahe cytometry. Ke koho nei i CD3+ T Cells Positive Selection no ka hoʻoulu ʻana a i ʻole cd3+ t cell isolation no ka wikiwiki, ʻo ka pololei o nā hopena ka mea nui.

Hoʻonui i ka CD8+ T Cell Isolation no nā haʻawina Cytotoxic

ʻO ke aʻo ʻana i nā pūnaewele "killer" cytotoxic T pono e hoʻomaʻemaʻe ikaika e hōʻoia i ka hana o ka effector e hōʻike maoli i ka heluna kanaka.cd8+ t hoʻokaʻawale ʻiaUa hoʻololi ʻia ma o nā ʻano magnetic pehea e ʻohi ʻia ai kēia mau cell mai nā laʻana paʻakikī. I nā hiʻohiʻona kahi e pono ai ka nui o nā cell,CD8+ T Koho Ponohāʻawi i kahi hopena paʻa a maikaʻi.

Inā pili ka noiʻi i nā transcriptomic koʻikoʻi,CD8+ T Hoʻokaʻawale ʻole ʻiaʻoi aku ka maʻamau e pale i nā hopena hōʻailona o ka hoʻopaʻa ʻana i ka antibody. ʻO kēia mau hana holomua i ka hoʻokaʻawale ʻana o cd8+ t cell, me ka CD8+ T Cells Positive Selection a me CD8+ T Cells Negative Isolation, e hoʻomau i ka hoʻokau ʻana i ko mākou ʻike i ke ʻano o ke kaua ʻana o ke kino i nā pathogens.

Hoʻohālikelike Hoʻohālikelike o nā ʻano hana hoʻokaʻawale T Cell

I ke koho ʻana i ka protocol kūpono, pono nā mea noiʻi e kaulike i ka maʻemaʻe me ka pono hana. ʻO ke koho ma waena o CD3+ T Cells Positive Selection, CD4+ T Cells Positive Selection, a me CD8+ T Cells Positive Selection me ko lākou mau hoa maikaʻi ʻole e hilinaʻi pinepine ʻia i ka naʻau o ka noi lalo.

Noʻonoʻo ʻenehana no ka hoʻokaʻawale ʻana i nā cell immunomagnetic

Hoʻomalu ʻia ka maikaʻi o ka hoʻokaʻawale ʻana o ka cell immunomagnetic e ka antibody binding affinity a me ka gradient field magnetic. Ma CD3+ T Cells Positive Selection, hōʻoia ka lepili pololei i ka kikoʻī kiʻekiʻe. Eia nō naʻe, ma CD3+ T Cells Negative Isolation, pono e hoʻomaʻamaʻa maikaʻi ʻia ka cocktail e hoʻopau i nā laina ʻē aʻe.

Pēlā nō, ʻo CD4+ T Cells Positive Selection a me CD8+ T Cells Positive Selection hāʻawi i ka pono o ka wikiwiki. Eia nō naʻe, no ka poʻe noiʻi e pili ana i ka hoʻōla ʻana, ʻo CD4 + T Cells Negative Isolation a me CD8 + T Cells Negative Isolation e hāʻawi i ka maluhia o ka noʻonoʻo e noho mau nā cell i kahi kūlana hoʻomaha. ʻO ka hope loa, inā ʻoe e hana ana i ka hoʻokaʻawale cell cd3+ t, ka hoʻokaʻawale cell cd4+ t, a i ʻole ka hoʻokaʻawale cell cd8+ t, ʻo ka hoʻololi ʻana o kēia mau ʻenehana e hōʻoia i nā hopena reproducible a scalable.