Kanaka a me ka holoholona holoholona holoholona o ka biological biological matrix no keʻano bionalyticalʻano hana bioanalyticical a me ka hōʻoia bionalytical

1 Iphose mau huahana

2 mau BAMAMATRIX

ʻAno likeʻole oNā Matricles biological, me nā koko āpau, nā serum, plasma, boile, ka waiū, nā mea aloha, nā mea kanu, nā mea hoʻonaninani, nā mea hoʻonaninani, e hoʻopiliʻia i nā mea ola kino.

3 ka pono o ka hoʻohanaʻana i nā Manchical Biological Matrices i nā haʻawina preclinical

In ʻO ka noiʻi mālamaʻana i ka lāʻau lapaʻau,ʻo ka biological biological he corsestone no ka hoʻokumuʻana a me ka hōʻoiaʻanaʻO nā hana loiloi biopical. ^. I ka hoʻohanaʻana i nā Matrices Faack i nā kumuhana olakino a iʻole nā ​​holoholona hoʻokolohua, hiki ke hoʻomau i nā hopena o ka calibration, a me nā hopena kūpono o kaʻohiʻana.

4ʻO keʻano o ka hōʻoiaʻana o keʻano a me ka hoʻokumuʻana a me ka noiʻana a me ka noiʻana o ka matrix biological biological

E like me nā koina e like me FDA / EMA i ka hoʻokumuʻana a me ka hōʻoiaʻana o nā hana loiloi BioBical Atrix e hōʻoia pono e hōʻoiaʻiʻo i ka pololei o ka loiloi. Nui loa ma LC - MS / MS / MS / MS / MS / nāʻokoʻa i nā kumu matrix mai nā kumuwaiwai'ē aʻe e pili ana i ka effiency likeʻole. Ua hoʻohana nuiʻia ka pākeke pale no ka hoʻomākaukauʻana i nā kīʻaha calibration, e hoʻomākaukau ana i nāʻano mana kūpono, entrax, entc. i nā hopena paʻa, nāʻano hana hoʻomohala. ʻO ka hopena a me ka hopena matrix o ke kaʻina hana e pono ai e koi i nā koi kiʻekiʻe kiʻekiʻe no ka matrix blank.

ʻO nā hiʻohiʻona noi o ka bilila o ka bilila bili i ka uluʻana o ka lāʻau lapaʻau

ʻO BILE PRILETITAL BILEX (simulated bile matrix)he hopena synthetic e simulates maoli e hoʻohālikelike i nā'āpana clale maoli a hoʻohanaʻia no ka lāʻau lapaʻau lāʻau lapaʻau, toxicology, a me ka noiʻi noiʻiʻanaBILE FAIL FACKEXHe waiwai nui ka noiʻana i nā mea e pili ana i ka lāʻau lapaʻau e pili ana i ka lāʻau lapaʻau, nā lāʻau lapaʻau e pili ana i nā lāʻau lapaʻau a hepatotomicism

6ʻO nā lāʻau hepatobiliary

I ka hoʻomohalaʻana o nā lāʻau lapaʻau no ka'ōnaehana hepatobililiary, bilila ble bleart e pāʻani ana i kahi kūlana simulackin (pk)

6.1 E loiloi i nā hiʻohiʻona o ka bila o nā lāʻau lapaʻau

Noiʻi ma nā kāpili hepatotintectition: bilila kekahi o nā ala nui no keʻano o nā lāʻau lapaʻau a me ko lākou metabolites. Ma ka hoʻohanaʻana i ka lāʻau lapaʻau maikaʻi,ʻo ka loliʻana o nā lāʻau lapaʻau e hoʻohālikelikeʻia ai ka loiloiʻana i ka hoʻopiliʻana i nāʻano hepatic.

ʻO BILE CLEIT CLEART CLEART e hoʻoholo nei

6.2 Ke aʻoʻana i ka hopena o nā bila o nā bile e pili ana i ka lāʻau lapaʻau e pili ana

Hiki i ka hopena Solubilization: Hiki i nā lāʻau lapaʻau ke hoʻolaha i ka neʻeʻana a me ka hoʻopiliʻana o nā lāʻau lapaʻau e like me ka momona

ʻO nā lāʻau lapaʻau lāʻau lapaʻau lāʻau lapaʻau: kekahi mau lāʻau lapaʻau (e like me Liraglutide

6.3ʻO ka loiloi loiloi o Hepatotikanile a me Choresasis

ʻO ka lāʻau lapaʻau lāʻau lapaʻau lāʻau lapaʻau

Hiki ke hoʻohālikelikeʻia nā mana biomchina: ma ka hoʻohuiʻana i nā'āpana kiko'ī e like me Bilirubin e hiki ke wānanaʻia ka palekana a me ka hopena.

6.4 E kūlike a me nā pono kūpono o ke hoʻololiʻana i ka bili

Ke kāhea nei i ka pilikia o ka Scpecle Scpec

Kūpaʻaʻo Batch:ʻO ka hoʻohuiʻana o ka Matrix Company

6.5 Kākoʻo i ka hoʻomohalaʻana o LC - Ms / Ms Bulogical Kūlana Kūlana

ʻO ka hoʻoponoponoʻana o Matrix:ʻO kaʻano paʻakikī o ka bile kūlohelohe (nā phospholipids kiʻekiʻe a iʻole ka hoʻonuiʻiaʻana i ka hale kūʻai. ʻO ka lāʻau lapaʻau bilaticalitial

7ʻO nā noiʻi noiʻi Cross Cross

ʻO ka hoʻohuiʻana o nā'āpana o ka bili i waena o nā holoholona likeʻole

7.1 Cynomolgus monkey bile (nhp bile)

ʻO ka pīpī o ke moni cynomolgus e like me nā kānaka e pili ana i nāʻano o keʻano he lāʻau lapaʻau non.

7.2 Bear bob

ʻO kaʻike phospholipid ma Beegle Boile e kiʻekiʻe loa, e kūpono ana no ke aʻoʻana i ka hoʻopiliʻana i nā lāʻau lapaʻau. Eia nō naʻe, nāʻokoʻa'ē aʻe ma ka hoʻopiliʻana i ka hoʻopiliʻana ma waena o Baile ma waena o Beagle a me nā kānaka e koi i ka calibration ma nā matala kūlohelohe.

7.3 SD RAM BILE

ʻO ka nui o ka hōʻiliʻiliʻana o ka pīpī o SD RED RATS (~ 1 ML / manawa) Eia nō naʻe, pono eʻikeʻiaʻo ko lākou billie kiʻekiʻe kiʻekiʻe e hoʻopiliʻia me ka LC - Ms / ms ka hoʻohanaʻana.

7.4 ICR / CD - 1 Mouse BILE

Loaʻa i ka CD - 1 Mouse i kahi bile bila liʻiliʻi (50 - 200 μ

7.5 C57bl / 6 Mouse Bile

ʻO C57Bl / 6 Mouse i hoʻohana pinepineʻia i nā hiʻohiʻona maʻi metabolic, a me nā loli i kā lākou pohō i hiki ke hōʻike i nā kūlana pathologial.

Pono e hui pūʻia nā hiʻohiʻona ma luna me nā mea i hoʻopiliʻia me ka pākēneka

Hopena

ʻO ka pākaha BILE FILET BILE, e like me ke kī nui no ka hoʻokaʻawaleʻana i ka hana o ka pīpī kūlohelohe, e pāʻani i kahi hana maikaʻiʻole i ka uluʻana o nā lāʻau hepatobililiary. Me ka hoʻoponoponoʻana i ka hoʻopiliʻana o nāʻano likeʻole (e like me nā'īlio cynomolgus, nā'āpana o ka pono o ka lāʻau lapaʻau.

Me ka hoʻolālāʻana o ka lāʻau lapaʻauʻelua,ʻo Bile Blei e hoʻohui hou i nāʻenehana paʻi a me nāʻoihana Microfluaid, e kākoʻo ana i ke kākoʻoʻoi aku ka hoʻomaikaʻiʻana i ka maʻi lapaʻau.

Nā hua'ōlelo nui:Black Blilogical InssionSsis, biological sample, bile ghile, missus chile

Kamaʻilio

Li t, calang jy. ʻO ka hōʻailona inoaʻo BILE ACD ACID ma ka maʻi metabolic a me ka lāʻau lapaʻau lāʻau. Pharmacol Rev. 2014 o Oct; 66 (4): 948 - 838. Doi: 10.1124 / pr.113.008201. PMID: 25073467; PMCID: PMC41180336.