Splenocytes ma ka noiʻi immunological

Nā Hua'ōlelo:Splenocytes (spls); ʻO ka mynomolgus Monkey Splenocytes; ʻO Rhesus Molisk Nāʻeha o ka'īlio; Nā maʻi catine; Nā'āpana kaulike; Nā kiʻi kiʻi; Nā mice splenocytes; Nā huapalapala o ka rabbit; E hoʻohālikelikeʻia Nā mea huna manuahi; Thawing splenocytes

Meaʻai Iphase

Splenocytes (spls)ʻO kahi heluna he oterogeneous o ka nui o nā mea i hanaʻoleʻia mai ka sopleen, a me keʻano o ke koko, a mālama i ka home. Hoʻokomo kēia mau pahu i nā lymphocytes (t cell, b cell), nā macrophages Ua hoʻohana nuiʻia nā stranocytes i ka noiʻiʻana a me ka hoʻokele limahana hana e aʻo i ka hanaʻana, wīwī,ʻo Tracerne Afsasses, a me CaniMonerapy.

ʻO ka hana a me ka hoʻohuiʻana o nā soplenocytes (spls)

I loko o ka pahu i loko, e noho ana nā soplenocytes i nā wahi ākea: ke kokoʻulaʻula, nā mea nui a me nā meaʻala keʻokeʻo Ua hoʻonohonohoʻia ka pulp keʻokeʻo i loko o nā kelepona a me nā belle belle Hanaʻia kēia mea hana pūnaewele i nā mea waiwai (SPL) i kahi mea ikaika i ka poʻe ivmunological e like me Elispotry, a hui pūʻo Lymprotry.

Nāʻano kiʻi kiʻi - Hōʻike kūikawā (SHLS)

-ʻO nā strillgus monkey tynomolgus:Hoʻohanaʻia nā hua'ōleloʻo Cynomolgus i ka noiʻi preclinical no ka meaʻoi aku ka nui o ke kanaka moni manu.

-ʻO ka mikiʻo Rhesus Monkey:ʻO nā mea iʻike nuiʻia e nā soples Shakey Splenocytes no ko lākou mau mea e pili ana i nā poʻe immunologic e like me nā kānaka. Nā haʻawina e hoʻohana ana i nā kiʻinaʻo Rhesus Mothes Soplery e kōkua i keʻano o ka gap ma waena o nā hiʻohiʻona holoholona a me nā noi.

-'Īlio tobsnocytes / canline splenocytes:Ma ke noiʻi Veterinary,'īlio Splernocytes

-ʻO nā soplemonicytes: He mea nui nā soplens i nā splenocytes i nā haʻawina ma ka hana antibody a me ke kūkuluʻana. ʻO nā soplemonicytes splenocytes kōkua e hōʻike i nā kiko'ī e like me kaʻokoʻa o nā kiʻi i nā kiʻi kiʻi a iʻole nā'āpana.

-ʻO nā kiʻi kiʻi kiʻi kiʻi / Mice Splenocytes:ʻO nā kiʻi kiʻi kiʻiʻia kekahi o nā hōʻike nui loa i aʻo pinepineʻia (SPL) ma Immunology. Ua hoʻohanaʻia nā protocols no nā mika ma keʻano he'ileʻeleʻele.

-Nā Hōʻalua:Hāʻawi i nā hua'ōlelo Rat Slenocytes e hāʻawi i kahi hoʻohālike hoʻohui no ka immunotomixology a me nā haʻawina hoʻomaha. Hoʻohālikelike pinepineʻia nā hua'ōlelo rat i nā kiʻi paʻi kiʻi e hoʻomaopopo i nāʻokoʻa likeʻole.

Splenocytes

SplenocytesʻO ke kaʻina hana o ka laweʻana i nā cell i ka nui mai ka spleen e hoʻohanaʻia ma nā noi noiʻi likeʻole, e like me keʻano o ke kahe cytmphocyt.

Ua laweʻia ka'ōlelo ma lalo o nā kūlana Aseptic, ka'āina i ka hopena o ka hoʻokaʻawaleʻana a hoʻoiliʻia i kahi pahu sterile i loaʻa iā RPMI 1640 Medium. Ua hōʻiliʻili ponoʻia ka papa Leukcyte ma hope o ke kenekulia. Ua holoiʻia nā cell me RPMI 1640 Memum, Cetrifiged a weheʻia ka Supernatant. Ua hana houʻia kēia manawa holoi holoi 1 - 2 mau manawa ma mua o nā papa hana i hoʻohanaʻia no nā hoʻokolohua hou aʻe.

Nā hōʻikeʻole

Nā hōʻikeʻolehe hana koʻikoʻi no ka mālamaʻana i nā cell no ka hoʻohanaʻana i ka wā e hiki mai ana. Hiki i ke cryoperestation keʻae i nā mea noiʻi e kūʻai i nā hōʻikeʻike no nā manawa lōʻihi me kaʻole o ka hoʻopiliʻana i ko lākouʻano a me ka hanaʻana paha.

ʻO ka supernatant o ka centrifuged cell centrified e hoʻokuʻuʻia mai keʻano o ka hana a ke keʻena i hoʻokuʻuʻia a ua hōʻemiʻia ka hana o ke kelepona. Hoʻohui i kahi aliquit i kēlā me kēia manawa kīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīʻia E hoʻokuʻu koke i nā mea hana manuahi manuahi ma A - 80 ° C FYZER. E hoʻololi i - 150 ° C Ferzer pahu pahu (a iʻole ka wai nitrogen Tank) no ka lōʻihi -

Thawing splenocytes

Thawing splenocytesPono e hana pono e hōʻoia pono e hōʻoia i ka mana o ke kelepona kiʻekiʻe a me ka hana ma hope o ka cryoporertration. ʻO ka hana maikaʻiʻole ke kaʻina hana e hōʻemi ai i nā hopena hōʻino o DMSO a me keʻano o ka crystal o ka hau.

Hoʻokuʻuʻia nā cryotubes i kahi wai 37 ° C wai wai a me nā lole a pau a me nā cryo cryo clielts i noho ai i nā'ōpala. E hoʻohui i ka 0.5 - 1ML o ka moʻomeheu cell clection i ka pahu pahu pahu, resuspend a hoʻololi i ka hoʻokuʻuʻana i kahi kula 15ML ʻO ka Campfuge, e wehe i ka supernatant a kaomi i ka Tube e wehe i ka hoʻonohonohoʻana o ka cell. E hoʻohui i 1ml o ka papa moʻomeheu cell, e puhi a hoʻoulu i ka pipette, hoʻohui i ka nui o ka nui o 15 ml. CamtRuFuge, de - Supernatize, hoʻohui i ka 1ml o ka cell clecture medium, hoʻohui pū me keʻano e like me keʻano o ka nānāʻana. E kau i nā cell i loko o kahi cop incubator a me ka incubate no 1h me kahi'āpana liʻiliʻi i loko o ka uhi. I ka pauʻana o ka incubation, resuspend a waiho aku no 1min eʻae i nā pālākiō a me nā lepo lepo. ʻO ka hoʻokuʻuʻana o ka cell subpension me kaʻole o ka neʻeʻana i ka hana houʻana i kahi 3ML TUBE 15ML. Heluʻia nā cells e hoʻoholo i ka hana cell.

Hopena

ʻO Splenocytes, me kā lākou huiʻana o kā lākou huiʻana a me ka pivotal a me ka pivotal i loko o nā pane immune Inā loaʻa i nā rodents, he nui, a iʻole nāʻano'ē aʻe e like me nā mea aloha a me nā'īlio, nā maʻi maʻi, a me nā maʻi maʻi maʻi, a me nā maʻi maʻi maʻi, a me nā maʻi lapaʻau ʻO nā kaʻina hana o ka nohoʻana, ka manuahi, a he mea nui ka thawing no ka mālamaʻana i kā lākou kūpaʻa a me ka viblity, e hiki ai i nā mea noiʻi keʻimi i nā hiʻohiʻona likeʻole. E like me kā mākou hoʻomaopopoʻana i ka Immunology Deepens