Advancement ma Bionialysis:ʻO ka hana o LC - Ms / ms ma Ophthalmic lapaʻau lāʻau lapaʻau

Iwapi aku

Ka wai chrisography - Tandem nui Spectrometry (LC - Ms / ms)

Wai chromatography - tandem nui spectrometry (LC - Ms / ms) He hana ikaika ikaika e hoʻohui ai i nā mana hoʻokaʻawale o ka chromatography wai me nā mana nui o ka helu helu helu. I LC - MS / MS / MS, i hoʻokaʻawaleʻia kahiʻano hoʻohālike e pili ana i keʻano o ka chromatograph. A laila eʻokoʻaʻo nā'āpana hoʻokaʻawale a me ka hoʻolimaʻana i ka tendm deptedleloʻo Tardem ma kaʻatikala

Nā noi o LC - Ms / ms ma Biohalysis

BooAhalySSsispili i ke anaʻana o nā lāʻau lapaʻau lāʻau, metabolite, a me nā mea waiwai'ē aʻe i loko o nā mea moʻomeheu kaulana, e like me ke koko, he plasma, ka meaʻala,biofliids. ^. LC - MS / MS / MS i kēia mau noi no kaʻike haʻahaʻa a me ka hiki keʻike i nā loiloi haʻahaʻa.

LC - Ms / msʻenehana no ka nānāʻana o nā kiʻi biopical eʻike i nā mea likeʻole a me nā mea i hoʻopauʻia. Nā mea noiʻi i hoʻohālikelikeʻia e ka hoʻohuiʻana i ka mea e hoʻopili ai i ka mea e anaʻia i apale matrixE hana i kahi helu helu helu helu helu helu helu helu a me kahi hiʻohiʻona maikaʻi. ʻO ka neʻeʻana o nā mea e anaʻia e anaʻia ma kahiʻano hua'ōlelo i hōʻailonaʻia e kahi curve maʻamau.

ʻO nā mea nui nā mea i loaʻa i nā mea maoli i hiki i ke kino. ʻO ka mea e hoʻopau ai i ka waiwai. Ua lilo nā lāʻau lapaʻau e pili ana i kahi ala nui no ka hoʻomohalaʻana i nā lāʻau lapaʻau hou i nā makahiki i hala. Me ka hānauʻana o ka nui o nā lāʻau lapaʻau nui me nā mea i pau i nā lāʻau resogenosus, ka bioanasis o nā lāʻau lapaʻau i lilo i mea nui aʻoi aku ka nui. Eia nō naʻe, i kēia manawa, ka hōʻoia o nā hana hoʻohālikelike o ka fday a me nā mea hana e pono ai a me nā mea kūpono. Mai kaʻikeʻana o kaʻikeʻana i nā mea i hiki mai i nā pilikia ma muli o kona hopena e like me keʻano heʻano maʻamaublalogical biological matrix (ʻO ka Matrick biological biologicial) E hoʻopau i kēia pilikia.

Papa 1:ʻO ka weheweheʻana i ke koho balota ma kaʻoihanaʻo Biotanaldical Medical

ʻO ka hoʻolālā kikowaena kikowaena kikowaena a me ka hōʻoiaʻana i keʻano

Ma Bionialysis, e hōʻoiaʻiʻo i ka hilinaʻi a me keʻano o nā hopena o nā hualoaʻa o nā kumu hoʻohālike. Pono kēia i ka hoʻomohala ikaika aʻO ka hōʻoia o ka loiloinā ala.

Hoʻomohalaʻano hana hoʻomohalapili i ka hoʻokumuʻana o nā kaʻina hana i makemakeʻia no ka nānāʻana a me ka heluʻana i nā loiloi o ka hoihoi. Hoʻokomoʻia kēia me ke kohoʻana i nā kūlana chromatographic o'ualelo (e.g., ka paeʻana, kailaʻana, a me nā helu heʻe, a me nā helu. Eia kekahi, pono keʻano o keʻano o ka hōʻikeʻana i nā loiloi ma mua o ka paʻakikī a me nā loli paha, a me nā mea hoʻohui'ē aʻe e hoʻopili ai i ka loiloi.

Hoʻokahi keʻano o kahiʻano, pono ia e ponoKa hōʻoiaʻana i keʻanoE hōʻoia e hālāwai me ia e hālāwai ai i nā kūlana hana i hala. Pono kēia kaʻina hōʻoia e hōʻoia e kūpono keʻano o keʻano he kumu kūpono no kona kumu i manaʻoʻia a koiʻia me nā koi regulatory. No nāʻano koho bionialytical, ka hōʻoia pinepineʻana e pili ana i kekahi mau hōʻailona nui:

• - Kūpono a me ka pololei:ʻO ka hōʻoiaʻana i keʻano e hāʻawi i nā hopena kūpono a me nā hopena kūlike.
• -ʻO ka hiki keʻike i nā haʻahaʻa haʻahaʻa o ka loiloi.
• - Kapuamua:ʻO keʻano o keʻano e hoʻokaʻawale ai i ka loiloi mai nā pūnāwai'ē aʻe i loko o ka Matrix.
• - Hoʻoiho:ʻO ka maikaʻi me ka mea e hoʻopiliʻia ai ka loiloi mai keʻano hoʻohālike o ka biological.
• -ʻO ka paʻaʻana o ka mea noiʻi ma lalo o nā mea e waiho ai a me nā kūlana lawelawe.
• - Lainawaena:ʻO keʻano o keʻano e hana ai i nā hopena i kūponoʻole i keʻano o ka loiloiʻana i kaʻikeʻana ma luna o kahi kiko'ī.

Blilogical biological Matrix a me nā matrix e pāʻani ai i nā hana koʻikoʻi ma kēia kaʻina hōʻoia. ʻO kēia mau hiʻohiʻona paʻa,ʻaʻole i loaʻa i ka loiloi o ka hoihoi, pono no kaʻikeʻana i nā hopena matrix a iʻole nā ​​hoʻonohonohoʻana i ka wā o ka nānāʻana. Kōkua lākou i ka hoʻokumuʻana i nā pae basline no nā loiloi a hōʻoia i ka hoʻonuiʻoleʻana o ka matrix iā iaʻaʻole e hāʻawi i ka hōʻailona. Pēlā nō, ka hoʻohanaʻana olāʻau lapaʻau - manuahihe mea nui no ka hōʻoiaʻana i nā lāʻau lapaʻauʻoleʻole a iʻole nā ​​metabolites a iʻole nā ​​metabolites i kēia manawa ma ka hoʻohālike e hiki ai ke skew i nā hopena.

Bionialysis of Ophthalmic Picles

Ua hoʻokaʻawaleʻia ka pā o kaʻili o kaʻili iʻekolu mau'āpana,ʻo ia ka papa i waho o ka membrane figorn; ʻO ka membrane waena o ka membrane puaʻa, vascular membrane a iʻole a me ka avaa; aʻo ka menemrane o loko. Ua maheleʻia kaʻili iʻelua'āpana, nā wahi ma mua a me nā wahi o ka maka o ka maka, paʻaʻia e ke kua o nā lens.

Kiʻi 1. Anatomy o ka maka kanaka.

ʻO nā papa nui e komo i loko o nā lāʻau lapaʻau lāʻau metabolism

• CORREEA-ʻO ka pūnaewele nui no ka piliʻana i ka lāʻau lapaʻau e pili ana i ka lāʻau lapaʻau e pili ana i nā mea nui a me nā mea e pili ana iā Cytochrome P450 (Cyp) e pili ana i nā mea hoʻolaha.
• Conjuction- waiwai i ka lāʻau lapaʻau - metabolilizing enzymes (e.g., nā manaʻo a me nā cyps), hāʻawi i mua ma mua o ka hoʻopili muaʻana.
• ʻO ka haʻahaʻa aqueous-ʻO ka hana metabolic hana akā e pāʻani ana i kahi role i ka laweʻana i ka lāʻau lapaʻau a me ka hoʻomaʻemaʻe.
• Vitroo- Ma Hiki i ka IntravitReal Injection hiki ke hana pololei i ka retina a hōʻemi i ka mea hoʻopiʻi i ka wā o ke kaʻeʻana. ʻO nā mea hana molecule liʻiliʻi eʻokoʻa koke, a me nā lāʻau molena nui i loaʻa i ka hapalua lōʻihi - Nā loli vitreous me nā makahiki i hoʻopilikia i nā lāʻau lapaʻau.
• Loko-ʻO Sclera he nui loa i nā lāʻau molena nui a me nā lāʻau lapaʻau e pili ana i ka sclera ma o ka sclera i ka nui o ka molecular. ʻO nā hōʻailona subconjunction eʻae i nā lāʻau lapaʻau e komo i ka choroid, akā ua paʻakikī ke kaʻina hana. Hoʻopiliʻo Scleal Melanin i ka lāʻau lapaʻau a pili i kona hoʻokuʻu a me ka lōʻihi o ka hana.
• ʻO ka'āina posterior- Ma Ua waiwaiʻia nā'āpana retrocular i ke kahe koko a me nā lāʻau lapaʻau e hoʻopauʻia ma o ke aniani o ke kino a iʻole ka lymph. ʻO nā mea kūʻai akuʻo Vascular Vascular i loaʻa i nā lāʻau lapaʻau e komo maʻalahi i ka wahi i waho, akā he mea paʻakikī ke hele i ka hopena o ka pimp Melanin - Hiki i nā lāʻau lapaʻau ke hoʻolōʻihi i ka lōʻihi o ka hana.

Aqueous haʻahaʻa a me keʻano haʻahaʻa

'ŌleloʻO ka haʻahaʻa aqueousavitreous haʻahaʻaʻO nā wai ocular ocular e pāʻani nei i nā kuleana koʻikoʻi i ka mālamaʻana i ka ikaika intracular, e hāʻawi ana i nā mea kanu, a ke hoʻoikaika nei i ka mālamalama optical. ʻO ka haʻahaʻa o ka haʻahaʻa, he'ūlū kūpono, kūlohelohe, e hoʻopiha ana i nā keʻena a me nā loina, a me nā kukui. ʻO ka hapa nui o ka haʻahaʻa a me ka hapa nui o ka cilisa i hanaʻia e ka ciliary kino, e kū i ka maka ma ke kihi a me ka jornea. ʻO kēia mau wai leʻa e pili ana i nāʻanoʻano, e komo pū me nā kānaka, nā maka, nā pāpale, a me nā mea'ē aʻe. Ua hōʻiliʻili pinepineʻia lākou me nā nui nui mai nā holoholona'ē aʻe a iʻole nā ​​loko.

ʻO kaʻakaʻaka aqueous ma waena o nāʻano

ʻO kaʻakaʻaka kanaka

'ŌleloʻO kaʻakaʻaka kanakahe mea maopopo, nā meaʻai maikaʻi loa -ʻO ka wai momona e mālama i ka hoʻoikaikaʻana a me ke kākoʻoʻana i nā hana metabolic o ka cornea a me nā leona. Ua hanaʻia e ka cilitary kino a kaheʻana ma o ke keʻena kālepa ma mua o ke kauʻana ma o ka laweʻana i ka mode trabecular.

ʻO kaʻakaʻakaʻo Monkey Aqueous

'ŌleloʻO kaʻakaʻakaʻo Monkey Aqueouslike like like me nā kānaka i keʻano o ka hoʻohuiʻana a me nā dinamics. Hāʻawiʻia i nā mea likeʻole ma waena o nā mea nui a me nā kānaka,No ke kanaka no ka mea nuilawelawe e like me kahi kuhikuhi koʻikoʻi no nā haʻawina Ophthalmic.

ʻO Rabit Anequeous Humor

'ŌleloʻO Rabit Anequeous HumorʻO nāʻokoʻa nui loa mai kēlā mau papa mua, kūikawā i kāna hana paʻa a me ka helu o ka huliʻana. Hoʻohana pinepineʻia nā poʻe uila ma OCular i ka noiʻi OCular,ʻoiai keʻano o nāʻano likeʻole. Pono e noʻonoʻoʻia nāʻano likeʻole.

ʻO ka haʻahaʻa haʻahaʻa ma waena o nāʻano

Kanaka hanohano kanaka

'Ōlelokanaka hanohano kanakahe gel - e like me ka waiwai i kauʻia i ka wai nui o ka wai, collagen, a me ka honuluni acid. Mālamaʻo ia i nāʻano OCular,ʻo Absorbs Shocks, a mālama i nā lawelawe no ka laweʻana i ka halihali hānai.

ʻO Monkey Vitreous Humor

'ŌleloʻO Monkey Vitreous Humore hoʻololi i kahiʻano like me keʻano o ke kanaka i kaʻakaʻaka kanaka, hananon -ʻO ke kanaka loiloi koʻikoʻihe kumu hoʻohālike koʻikoʻi no ke aʻoʻana i nā makahiki.

ʻO Rabbit Vitreous Humor

'ŌleloʻO Rabbit Vitreous Humorheʻokoʻa kūpono,ʻo ia ka nui o ka wai - e like me ka haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa. ʻO kēia mauʻokoʻa ka hopena o kāna pane i nā hana hoʻohālikelike a me nā lāʻau lapaʻau lāʻau lapaʻau.

ʻO ka hoʻomohalaʻana o nāʻano kūlohelohe

ʻO keʻano nani a me keʻano a me keʻano hoʻowahāwahā

ʻO kaʻakaʻaka aqueousaʻO keʻano hoʻowahāwahā maikaʻiUa hoʻolālāʻia nā mea hana i hoʻolālāʻia no ka hoʻohanaʻana ma Ophthalmic Surgeries ʻO kēia mau mea hana synthetic mimik i ka biochemical a me nā mea pilikino o ko lākou mau ceffarts kūlohelohe.

Simulated antiumous a simular vitreous haʻahaʻa

Simulated Amoorasimulated vitreous haʻahaʻahe laboratory - Ua hoʻohanaʻia nā lako hana i hoʻohanaʻia no ka hoʻokolohua vitro a me ka hoʻohālikeʻana o ka mysiology ocular. Hoʻopili lākou i nā haʻawina i hoʻopaʻaʻia me kaʻole o nā mea kūpono e pili ana i nā holoholona a me nā hiʻohiʻona kanaka.

Hopena

ʻO ka hoʻohanaʻana o ka cromatohi koble - tandem ka mea nui loa (LC - MS) i ka pili kālā nui, me nā lāʻau lapaʻau a me nā metabili ʻO keʻano kiʻekiʻe o keʻano, pololei, a me ke koho a me ke koho e hoʻonuiʻia ma nā hōʻike waiwai a me ka hoʻonuiʻana i kaʻoihana lāʻau ophthalmic. ʻO kaʻike kiko'ī o keʻano ocular a me ke kuleana o nā wai e like me ka mea nui a me nā mea hoʻonaninani a me nā mea hoʻonaninani a me nā'ōnaehana o ka lāʻau lapaʻau o kēia mau kino Eia nō naʻe,ʻo ka hoʻomohalaʻana o nā mea hoʻonaninani a me nā mea hoʻonaninani ocular a simulat Ke hoʻomau nei ka hōʻoiaʻana o ke kaʻina hana ma mua o ka hōʻoiaʻana, e hōʻoiaʻiʻo i ka hilinaʻi a me ka hana hou i nā noi olakino e pono ai no nā noi olakino maikaʻi, kūikawā ma Ophthalmology.

Nā hua'ōlelo: LC - MS / MS / MS, BILLY ATRAIL, CHANTORLY GILYTION, CHANYYSILY ACTIORILS, MILYINY SWIMOR, CILRYINY SWITOR, CULRYINY SWITOR, CILYY VITROADILY CHANTOR, CILYINY SWIMOR, CILYINY SWIGNUS, CINALYY SWIMOR, CILYINY SWIGNY SWITOR, CULRYINY GRAMOR, CILYY VITROADY GRAMOR, CILYYY SWIMOR, CILYYY SWIMOR, CIRYINY SWIMOR, CIRALY VITROAD SWITY ʻO Rabbit Vitreous Humor, non - Na ke kanaka no ke kanaka vitreous haʻahaʻa,Simular Amorous Humor, simular Vitreous Humor, Artout Aqueous Humor

Kamaʻilio

Sayedpour, S. M., la na'āina, L., rezazaemoma, G. (2023). ʻO ka hoʻohālikeʻana o ka hoʻohālikeʻana o ka paneʻana o ka dinamic o kahi mea i hoʻonuiʻia i hoʻonuiʻia i ka stepor.Ana, 30, 100936. HTTPS://DOi.org/10.1016/J.MeAsen.2023.100936