Kumu o ka Comet Assay
ʻO ka Comet Assay, i ʻike ʻia he single-cell gel electrophoresis, he ʻano hana no ka hoʻoholo ʻana i ka genotoxicity ma ka ʻike ʻana i ka pōʻino o ke kaula DNA. Hiki iā ia ke ʻike pono a hoʻopaʻa ʻia i ka nui o ka pōʻino o ka DNA hoʻokahi a me ka lua i ka pōʻino i loko o nā cell. Ke hoʻoulu ʻia nā mea ʻino DNA endogenous a exogenous i ka haki ʻana o ke kaula DNA cellular, ua pōʻino kona ʻano superhelical, a ma lalo o ka hana a ka cell lysate, ua pōʻino ka membrane cell, ka nuklea nuklea a me nā hale membrane ʻē aʻe, a me nā protein intracellular, RNA, a me nā ʻāpana ʻē aʻe i loko o ka cell lysate.Ma lalo o nā kūlana kūʻokoʻa, hiki i nā ʻāpana DNA ke komo i ka gel a neʻe, ʻoiai ma lalo o ka hana a nā electrolytes alkaline, ua de-helicalized ʻo DNA a hōʻino ʻia nā kaula i haki ʻia a hoʻokuʻu ʻia nā ʻāpana. Ma muli o ka liʻiliʻi liʻiliʻi o kēia mau DNA a me ka denaturation alkaline i nā kaula hoʻokahi, ua haʻalele nā DNA i hoʻopiʻi ʻole ʻia i ka DNA nuklea e neʻe aku i ka pole maikaʻi i ka wā electrophoresis, e loaʻa ai kahi kiʻi comet-like, ʻoiai ʻo nā ʻāpana DNA i pōʻino ʻole e noho pōʻai. No laila, hiki ke hoʻokaʻawale hou ʻia i loko o ka hoʻāʻo comet neutral (PH=8.4) a me ka hoʻāʻo comet alkaline (PH>13) e like me nā waiwai pH like ʻole o ka electrophoresis buffer. Hoʻohana nui ʻia ka mea mua no ka ʻike ʻana i ka pōʻino o ka haki ʻana i ka DNA pālua-strand, ʻoiai ʻoi aku ka naʻau o ka hope a hiki ke hoʻohana ʻia no ka ʻike ʻana i ka liʻiliʻi liʻiliʻi o ka pōʻino hoʻokahi-strand a pālua-strand breakage.
ʻO ka mea maʻamau, ʻoi aku ka nui o ka pōʻino o ka DNA, ʻoi aku ka nui o ke kaula a me nā ʻāpana ʻāpana i hana ʻia, ʻoi aku ka liʻiliʻi o kona lōʻihi, ʻoi aku ka nui o ka DNA e neʻe ai ma lalo o nā kūlana electrophoretic like, a ʻoi aku ka lōʻihi o kona neʻe ʻana. No laila, hiki ke hoʻoholo ʻia ke kiʻekiʻe o ka pōʻino o DNA i loko o nā pūnaewele hoʻokahi ma ke ana ʻana i ka density optical a i ʻole ka lōʻihi o ka ʻāpana neʻe o ka DNA, pēlā e hoʻoholo ai i ka pilina ma waena o ka hopena hana o ke kumuhana a me ka hopena pōʻino DNA.
Nā huahana IPHASE
ʻoluʻolu: Hāʻawi ʻia nā reagents a me nā mea hoʻohana no ka electrophoresis gel cell single a hiki ke hoʻohana pololei ʻia, e hoʻopōkole loa i ka pōʻai hoʻokolohua.Pono: Ua hoʻokō ʻia kēlā me kēia ʻāpana o ka pahu i ka hoʻāʻo maikaʻi ʻana, a pololei nā hopena hoʻokolohua, hilinaʻi a hiki ke hana hou ʻia.
Paʻa: paʻa ka pahu a maʻalahi ke lawe ʻia a mālama ʻia.
Ka hoʻohana ākea: Loaʻa iā ia ka nui o nā noi a hiki ke hoʻohana ʻia no ka hoʻāʻo ʻana i ka genetic toxicology o ka meaʻai, nā mea kemika, nā pesticides, nā disinfectants, nā mea hoʻohui meaʻai, nā mea hoʻonaninani, nā mea lapaʻau a me nā mea ʻē aʻe.
Ua komo nui ʻo IPHASE ma ke kahua o ka noiʻi ʻepekema reagents no kekahi mau makahiki, a ua hana ʻo ia i ke ʻano o ke keʻa - kahua a me nā mea hana noiʻi ʻepekema kiʻekiʻe-hope me ka ʻoihana maikaʻi loa a me ka ʻuhane hou. Hāʻawi ʻo IPHASE i nā mea hana hoʻomaʻamaʻa kūpono a maikaʻi e hoʻolōʻihi i ka hānau ʻana o nā lāʻau hou; He hoku ʻālohilohi nō hoʻi ia i ka nui o ka ʻimi ʻana o ka ʻepekema ola, e hāʻawi mau ana i nā ʻepekema me nā mea hou, nā hoʻolālā hou a me nā ʻāwī hou, e alakaʻi ana i kēlā me kēia lele i nā palena o ka noiʻi ʻepekema. Ma ke kahua paʻakikī o ka noiʻi genotoxicity, ua hōʻike ʻo IPHASE i ka ʻike kupaianaha a me ka hoʻokō ʻana, e hoʻomaka ana i kahi ʻano o nā hopena kūpono a maikaʻi hoʻi e hoʻomaʻamaʻa i ke kaʻina noiʻi paʻakikī.
Ka manawa hoʻouna: 2024-07-26 15:29:08