Mawu OyambaMagnetic adayendetsa maselo
Ma Magnetic Octing Orting Cell (Macs) ndi ukadaulo wofunika m'munda wa Cell Biology ndi kafukufuku wa biomidel. Monga mmwamba - Ma Macilidwe Amitundu Yosanja, Mac amathandizira ofufuza kuti aletse mitundu yachindunji ya maselo amitundu yosiyanasiyana, ndikupereka chida chofufuzira komanso matenda. Opangidwa kuti musinthe luso komanso kusanjana kwa maselo, luso limagwiritsa ntchito mikanda yamatsenga yomwe imapangidwa ndi ma antibodies makamaka kuti aletse maselo.
Mfundo Zomwe Amakhala Ndi Macs Technology
● Zoyambira zolekanitsa
Mfundo yofunika kwambiri ya ma Macs imakhazikitsidwa pamagnetic. Njirayi imaphatikizapo kugwiritsa ntchito tinthu ta maginito, nthawi zambiri nano - mikanda yophatikizika yokutidwa ndi ma antibodies, kuti amange zilembo zapamwamba. Pamene zitsanzo zosakaniza zimayenda kudutsa mu mzere mkati mwa maginito, maginito - Maselo olemba olembedwa amasungidwa, pomwe omwe ali - maselo olembedwa olembedwa amadutsa. Kulekanitsa kumeneku kumatheka popanda kukhudza luso la maselo, ndikupangitsa kukhala chisankho chabwino kwa anthu omwe ali ndi chidwi.
● Ntchito ya ma antibodies mu cell
Ma antibodies amatenga gawo lofunikira kwambiri mu maginito - kukonza maselo. Amatsogolera kuwongolera kwa mikangano yamatsenga kupita ku zolembera zam'madzi za Desges, ndikuwonetsetsa kuti maselo omwe amafunidwa ndi okhawo. Kusandulika kumeneku ndikofunika kuti mupeze - zitsanzo za zitsanzo, zofunikira pakugwiritsa ntchito mu kafukufukuyu komanso zamankhwala.
Zida zomwe zimagwiritsidwa ntchito m'macs
● Kufotokozera za Mac
Macs Columns ndi ofanana ndi kudzipatula. Ming'alu iyi imayikidwa mkati mwa maginito, kulola maginiki - maselo olembedwa kuti asungidwe pomwe osasungidwa omwe si - maselo olembedwa amasungidwa. Mitundu yosiyanasiyana ya courm imapezeka, yosiyanasiyana kukula ndi kuthekera kokhala ndi mavidiyo osiyanasiyana ndi manambala a cell.
● Mitundu ya maginito
Kupambana kwa Macs amadalira kwambiri mikangano yamagetsi yomwe imagwiritsidwa ntchito. Mikanda iyi imapangidwa ndi mitundu yosiyanasiyana kuti igwirizane ndi mawonekedwe osiyanasiyana, kuphatikiza kukula kwa bead, mphamvu zamatsenga, ndi zida zokutira. Kusankha mkulu - Magnetic Harnetic - Kupanga cell kugwirizanitsa kwa cell kumatsimikizira ntchito zoyenera komanso zotsatira.
Njira ya Magnetic - Kusintha kwa maselo
● Gawo - ndi - Njira
Njira za ma Macs ndizowongoka kwenikweni zimafunikira kulondola. Zimayamba ndi kulembedwa kwa maselo, komwe maselo amaphatikizidwa ndi mikanda yamagetsi yopangidwa ndi ma antibodies enieni. Maselo olembedwa amayambitsidwa m'magulu a Macs pansi pa maginito. Ma cell a - Ma cell arting woyamba, ndipo atachotsa mzerewu kuchokera ku maginito, maselo omanga amasonkhanitsidwa. Njira iyi, ikachitika molondola, imapangitsa kukolola kwakukulu kwa maselo okwera.
● Kukonzekera zitsanzo ndi kusamalira
Kukonzekera koyenera ndikofunikira kwa ma Macs opambana. Izi zimaphatikizapo kuonetsetsa kusanjana kwa mmodzi - Kavalidwe ka khungu, kuchotsa anthu okalamba, ndikusintha zozunzira maselo am'manja. Kugwiritsa ntchito maluso ayenera kuchepetsa nkhawa ya maselo ndikukhalabe ndi vuto, makamaka kwa mitundu yam'manja ngati maselo a tsinde.
Ntchito za Macs mu kafukufuku ndi mankhwala
● Gwiritsani ntchito kafukufuku wa khansa
Macs amagwiritsidwa ntchito kwambiri kafukufuku wa khansa kuti alenkhetse maselo a khansa osowa kwambiri kuchokera pa zitsanzo za magazi, pothandiza kuzindikira ndi kuwunika kwa mankhwala. Mwa kuperekera - zitsanzo za zitsanzo, ma Macs amawonjezera kulondola kwa kusanthula kwa pansi, monga kutulutsa kwa cytometry ndi ma talecular.
● Mapulogalamu mu trim cell cell
Kutha kudzipatula kumbali ya maseche ya maseche kumapangitsa macs patsogolo mu mankhwala oletsa kubereka. Imathandizira kuletsedwa maselo a tsinde pamafunika chithandizo chamankhwala, kuonetsetsa kuti machitidwe onse ndi otetezeka komanso ogwira mtima.
Ubwino wa Macs pa Njira Zina
● Kukhala ndi luso labwino komanso kuchita bwino
Poyerekeza ndi njira zina zopangira maselo, ma Macs amapereka mwachindunji ndi luso. Kugwiritsa ntchito ma antibodies apadera kumatsimikizira kuti maselo ozungulira okha ndi omwe amangodzipatula, kuchepetsa chiopsezo chokhala ndi mitundu yosafunikira ndikuwongolera zomwe zikuyeserera.
● Kuyenda modekha
Macs amadziwika kuti amayendetsa bwino maselo, ayenera kusunga umphumphu ndi kutchuka. Mosiyana ndi zodekha za ma harsher, ma macs amathandiza maselo m'njira yomwe imasunga boma lawo, ndikuwapangitsa kukhala oyenera kufufuza kapena kugwiritsa ntchito njira.
Zofooka ndi Zovuta za Macs
● Kutha kwa non -
Vuto limodzi ndi Macs ndiye kuthekera kwa malo omwe si - komwe kumangirira, komwe mikads imalumikizira maselo osakonzekera. Izi zitha kusungidwa pogwiritsa ntchito ma mbiya apamwamba kwambiri ndikulandila antibody.
● Kuganizira
Ngakhale othandiza kwambiri, mamanja amatha kukhala okwera mtengo kuposa njira zina, makamaka chifukwa cha mtengo wamatsenga ndi zida zapadera. Ofufuzawo amayenera kulemera ndalamazo motsutsana ndi zabwino za kukwera - kuyeretsa maselo oyeretsa.
Kupita patsogolo kwaposachedwa m'matumbo a Macs
● Kupanga kwa maginito
Kupita patsogolo kwapaku zaposachedwa mu ukadaulo wa Bead kwalimbikitsa macs. Mapangidwe atsopano amapereka mphamvu zamatsenga bwino komanso kulimbitsa thupi, kulola kusanja mwachangu komanso kuyera kwapamwamba.
● Kusintha kwa protocol
Kupita patsogolo kwa prococol chitukuko chakhazikika ma Macs, kuchepetsa nthawi zosanja ndikuwongolera bwino. Kusintha kumeneku kumapangitsa Macs kukhala ochulukirapo komanso osangalatsa kwa ma laborator angapo.
Kuyerekeza Macs ndi Njira Zina Zosanja
● Malumescence - Kukhazikitsa kwa maselo (kumaso) vs mac
Pomwe kuli njira inanso yotchuka, Macs imapereka zabwino monga zida zosavuta komanso zovuta zotsika. Mosiyana ndi nkhope, zomwe zimafuna njira zovuta, Macs amagwiritsa ntchito maginito otsika, zomwe zimapangitsa kuti zizipezeka.
● Ubwino ndi zovuta za aliyense
Njira iliyonse yosinthira ili ndi zabwino zake komanso zowawa zake. Nkhope imapereka kusanthula kwatsatanetsatane mwatsatanetsatane komanso kuli kokwera mtengo komanso nthawi yotanula. Macs, komabe, amapereka mwachangu, mtengo wake - Kusanja mwaluso ndi zofunikira zochepa kwa maluso, zabwino pazogwiritsa ntchito.
Maganizo amtsogolo komanso zochitika zomwe zingachitike
● Zochita zomwe zimachitika mu cell
Monga momwe kufunikira kwa mankhwala kumakula, matekinoloje ngati ma may apitiliza kusintha. Kuchitika mtsogolo kumatha kuphatikizira chabe, kuchepa, komanso kuphatikiza ndi njira zina zowunikira zopezera kusanthula kwa masele am'manja.
● Zosintha m'mabuku a Macs
Kugwiritsa ntchito bwino kwambiri muukadaulo wa Bead ndi masitepe opangira zitsamba zikuluzikulu. Kuphatikiza apo, kuphatikiza mapangidwe a deta ndi bioinformaticts kumatha kukulitsa ma eyamu omwe amapezeka kuchokera ku maselo am'manja.
Mapeto
Magnetic - Njira Yosanja Ma cell imakhala ngati njira yosinthira m'malo a pa cell biology ndi biomedicine. Chizindikiro chake chachikulu, chothandiza, komanso kugwiritsa ntchito mofatsa zimapangitsa kuti ikhale yosankhidwa kwa mitundu yosiyanasiyana ya maselo mu kafukufuku wina komanso kugwiritsa ntchito mankhwala azachipatala. Kusankha Wamkulu - Magnetic Ordic - Wopanga ma cell, wopanga mawonekedwe, kapena wotsatsa ndi wofunikira pokwaniritsa zotsatira zabwino ndi ma MaCS. Pamene ukadaulo umapita patsogolo, Maics apitilizabe kusewera mbali yokopa chidwi popititsa patsogolo kafukufuku wa biomededical komanso njira zochizira.
●Iphase: Wokondedwa wanu wodalirika
Kumpoto Mu North Wales, Pennsylvania, a Iphase bioscies ndi "katswiri, komanso zatsopano - zatsopano - bizinesi ya Tech. Kampani yathu imagwirizanitsa kafukufuku, chitukuko, kupanga, kugulitsa, ndi ntchito zaukadaulo zopanga zachilengedwe. Ndi chidziwitso chokwanira komanso kukonda kafukufuku wasayansi, gulu lathu lodzipereka limatipatsa zolengedwa zapadziko lonse lapansi, kuthandiza ofufuza kuti akwaniritse zolinga zawo zasayansi. Iphase yakhazikitsa malo angapo a R & D kutsitsa china, United States, Europe, ndi East Asia. Monga mtsogoleri mu - Vitro Biogical Reagents, Iphase Bedles odzipereka popereka zinthu zabwino zopita patsogolo kafukufuku.
Post Nthawi: 2024 - 10 - 26 166:42:41