Cgt yatsopano mankhwala - Lentivirus - Candsang Galimoto - TV

Cgt yatsopano mankhwala - Lentivirus - Candsang Galimoto - TV

Mphepo yamtengo wapatali mankhwalawa ndi mankhwala abwino omwe amathandizira chitetezo cha mthupi la thupi kuti chingachitike ndikuchotsa maselo a khansa. Mafomu ofunikira amaphatikizapo cytokine - mankhwala osokoneza bongo a cell, t - mankhwalawa cell, ndi galimoto - mankhwala a t cell. Galimoto - T mankhwalawa amaphatikizaponso ma cell a T elct exprect receptors (mgalimoto) yomwe ikuletsedwa makamaka maselo otupa ngati khansa ya heukemia ndi lymphoma.

Mawu osakira: Galimoto - T, T, lendivil, vekivil vertor, tracer lentivirus

Kukonzekera kwagalimoto - T

Mwambiri, njira zazikulu zokonzera galimoto - ma ma cells ali motere:

Chithunzi: Dana - Cancer Institute - Can T - Mayesero azachipatala amathandiza odwala ambiri a mmeroma abwerera ku thanzi

1. 1.Porting (t - kudzipatula

Gawo Loyamba Pokonzekera Galimoto - T ndikupatula T - maselo ochokera m'maselo oyera m'magazi a wodwala. Njira monga immunomagnetic Bead Kulekanitsidwa kapena Kutulutsa cytometry nthawi zambiri kumagwiritsidwa ntchito pochotsa CD3 + - Kulemera T - Maselo Ochokera M'magazi. Kuonetsetsa kuti ma cell, njira yosinthira imafunikira kwambiri, kuonetsetsa kuti chiwerengero chokwanira - chabwino t - Maselo amapezeka.

2. 2.Gaut (T - Kuyambitsa kwa cell)

Maselo otengedwa - Maselo amayambitsidwa mu vitro, nthawi zambiri pogwiritsa ntchito aCD3 / CD28 T - Ma cell actives / Expfanson Kit. Maselo oyambitsidwa ndi ma T Lowani mgawo, akuwakonzekeretsa kuti akwere. Kudzera mu njirayi, ntchito ya cell imakulitsidwa, ikuthandizira kusinthana kwa majini ndi kutsatsa.

3. 3.Transkens (gene wosintha)

Maselo oyambitsidwa ndi T amapezekanso kufotokozera Chitsulo antigen (galimoto). Njira iyi imagwiritsidwa ntchitoVectol verctorkuyambitsa majini agalimoto kukhala ma cell. Lentivirus ndi chida chogwiritsira ntchito matenda, ndi mwayi wa modetsa kukula kwa Genter (monga mtundu wagalimoto) kulowa mu cell genome. Ndi kupatsirana T ma cell nes mu cell Dona, Lentivirus amathandizira ma cell a T kufalitsa magalimoto nthawi yayitali - nthawi. Mbiri yagalimoto imaphatikizanso imodzi - Chidutswa chosinthika (scfv) cha ma cell a T amazindikira ma antigens pamtima ndikuyambitsa chitetezo cha T. Monga taonera Chithunzi 2. Kumasulidwa kwa lentivil kuli ndi mawonekedwe othandiza kwambiri komanso kukhazikika, kotero kuti mtundu wagalimoto ukhoza kufotokozedwa m'maselo a T nthawi yayitali.

Chithunzi patsamba: Singapore Galimoto - T

4. 4.Kufalitsa (kuchuluka kwa cell)

Pambuyo pothera, galimoto - ma cells amalowa mu The Empniction Station, komwe katundu mongaIl - 2amawonjezeredwa kulimbikitsa kuchuluka kwa maselo. Cholinga cha njirayi ndikuwonetsetsa kuti chiwerengero chagalimoto - ma ma cell t alipo kulowetsedwa kwina kwa wodwalayo. Njira yowonjezera iyenera kuchitika pansi pa chosabala kuti mutsimikize bwino maselo ndi kuchuluka.

5. 5.Kuwongolera (QC)
6. Asanatsimikize wodwala, kuwongolera kwapadera ndikofunikira pakukonzekera. Kukonzekera galimoto - ma cell a T akumayezetsaOnetsetsani kuyera, zochita zawo, kusinthika kwa magazi, komanso kutsatira malamulo a mankhwala. Kuphatikiza apo, kuyesa kwa kapangidwe ka maselo kapena kusangalatsa ndikofunikira kuonetsetsa kuti ndi achire komanso achire bwino kwa maselo atalimbikitsidwa kwa wodwalayo.

IKutumiza kwa Lentivirus Transtal Product cell

Tekinoloje ya Lentivil Transmural ndizofunikira kwambiri pa cenec mankhwala, makamaka kusinthira kwa ma cell. Ma vetoni a lentiviral, ochokera ku hiv - 1, amagwiritsidwa ntchito kwambiri chifukwa cha kuthekera kwawo kophatikiza zitsulo, ndikuwonetsetsa zokhazikika. Amatha kupatsirana mbali zonse ziwiri komanso zosakhala - Kulekanitsa maselo, ndipo chitetezo chawo chakhala chikusintha ndikusintha majini othandizira kuti atetezedwe. Malondawa tsopano amagwiritsidwa ntchito mu gene mankhwala, Vene posintha, ndipo kafukufuku wa cell biology kuti awonongeke ndi kulimba kwa mtundu.

Chithunzi patsamba: obiosh.com/product/ schemuc chojambula cha Lentivirus Pack ndi Lentiivirus Translation Play 293T maselo

1.

Verivil Vector imatha kupulumutsa mokwanira majini agalimoto, kukhala ngati gene yagalimoto, mu ma cell agalimoto komanso ma cell a curnes ndikuphatikiza kuti mtundu wagalimoto umafotokozedwa mosalekeza pogawika kwa maselo ndi kuchuluka. Kutali - kuthekera kochokera ku malo osinthira ndikofunikira kuti umvumbo uziyenda bwino.

2. 

Poyerekeza ndi ma veral ena, ma lentivirses ali ndi Imnogenicity wotsika. Zotsatira zake, pamene lentivisis amagwiritsidwa ntchito kutulutsa ma cell amthupi, chitetezo cha mthupi la wodwala chimatulutsa matenda ofooka kwambiri ku virus. Izi zimathandiza kuchepetsa mavuto olakwika, makamaka munthawi yayitali - kupulumuka kwa maselo pambuyo kulowetsedwa, potero kupewa kukanidwa kwathunthu.

3.Hitsani kusinthidwa kwa ma cell a mthupi

Vekitala wa lentivil umatha kutanthauzira mokwanira mitundu yosiyanasiyana ya maselo amthupi,makamaka ma cell ndi maselo a NK. Kudzera mu lentiviliction, ma cent ma cell amatha kupeza chotupa - maluso achidziwitso, poyankha amalimbikitsa kuthekera kwawo kukafuna ndikupha maselo otupa.

4.Preisiizitsani maselo osinthika omwe amafunsidwa 

Ku AMinotherapy,Maselo ogwiritsa ntchito ma cellFotokozerani za mthupi wa mthupi womwe, pambuyo poti transpostion, fotokozerani galimoto ndikukhala ndi anti - chotupa cha chotupa. Ma vesi a lentiviral amatha kutumiza ndendende majini agalimoto mu maselo awa, zomwe zimawapangitsa kuzindikira ndikuyang'ana maselo otupa.Ma cellKodi maselo otupa omwe amadziwika ndikuwonongedwa ndi ma cell a chitetezo awa. Kudzera mwa kutsegula kwa lentiviliction, ma cent ma cell amatha kukulitsa kudziwika kwawo pakuzindikira ndi kupha thukuta, potero kumapititsa kuchisomo.

Munkhani ino, Iphase, mtsogoleri wa vitro, wapanga lentivas pa Kit ndi Lentiivirus Getrict Stcierction Makasitomala A Immunotheraps ndikusintha kupambana kwa matenda okhazikika. Izi zimapatsa imodzi - siyani yankho la makasitomala.

Iphase LentivirusPmaufumu

A Ifa Lentiir Patch Kitili ndi ma regents onse ofunikira ofunikiraMasamba a Lentivirus, kutanthauza kwambiri kuyesera ndikupulumutsa nthawi ndi khama. Izi zikuphatikiza:

• Lentivirus Packging Plasmid Sakanizani
• Kutanthauzira kosinthika
• egfp plasmid
• Iphase Lentiivirus Woyang'anira Exenter

Khoti ili limaletsadi kuyeserera! Popanda kufunika kokonzekera kovuta, kuwunika Lentivis kumatha kumaliza bwino pongotsatira malangizo omwe akuphatikizidwa.Ubwino wapadera wa zokomera umaphatikizapo nthawi yayifupi, Chaira Lokwera Kwambiri, komanso kugwira ntchito kosavuta, kumapangitsa kuti zikhale bwino kwa oyamba kutsanulira Lentivil. Each set of reagents undergoes strict quality control to ensure accurate, reliable, and reproducible results, significantly improving transfection efficiency and the stability of viral vectors, thereby providing potent technical support for immune cell preparation.

Kuphatikiza apo, kuthandiza makasitomala kupulumutsa ndalama, ifase adapanganso ndikupanga lentivirus nensiction ntchentche, ndikupereka yankho lofulumira komanso losavuta.Pongosakanikirana ndi lentivirus subrents yolimbana ndi kugwedeza kwa ndende ndipo centrifugation pogwiritsa ntchito centrifuuge, tinthu tating'onoting'ono tingakhale olimbikira kwambiri. Njirayi siyifuna kuperekera, ndikupangitsa kuti ikhale yabwino komanso yotsika - njira yothandiza kwa ogwiritsa ntchito a labotale ambiri.

Chidule Ndi chinthu chofunikira kwambiri choyesera, kafukufuku wopatsa chidwi, ndi madera ena ogwiritsa ntchito.

Mwachidule, immunotherapy, makamaka galimoto - T Cell ndi Car - ma cell a NK GELOS, akuimira njira yatsopano ya chithandizo chamakono cha khansa. Kudzera njira y monga kusintha kwa maselo, kutsegula, kutsegula, kupatsa, kukulitsa zinthu zapamwamba, zomwe asayansi angakonzekeretse mankhwalawa, zomwe asayansi imatha kukonza chithandizo cha khansa kwa odwala, zomwe zimathandizira khansa.Kusankhidwa kwa lentivilis, ngati ukadaulo wapakati, umagwira ntchito yofunika kwambiri pokonzekera galimoto - T ndi galimoto - maselo a NK chifukwa cha kuchuluka kwake komanso kukhazikika kwake.Ndi kupita patsogolo kosalekeza kwaukadaulo, kumapangitsa kuthekera kopereka njira zothandizira odwala ambiri, kumayambitsa phindu lalikulu. M'tsogolomu, Immunurapy akuyembekezeka kubweretsa kusintha kwa matembenuzidwe a khansa kwambiri kwa odwala khansa.

Kuti tikwaniritse zosowa za gene, mawu, ndi kafukufuku wa matenda, Iphase imapereka Lentitis Packs Twagration Kits, ma repints a Lentivisi, omwe amatulutsa trantivirus. Trantiviruses a trantivirus amanyamula ndi fluorescent protein (gfp), ofiira a fluorescentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcentcent la Protery Shetry, ndikuwonetsa kutsata kosavuta kwa maselo ojambula ku Vitro ndi VIVO.

Kupatula apo, Iphase amathanso kupereka kawiri pre - senti yamalonda yam'madzi: GFP & Lucrifer & Mocher & Luciferass. Ogulitsa izi amatha kusankhidwa kapena kuphatikiza poyesa zoyeserera, makope ophatikizika ndi kulimbikitsa mwaulemu - Kutsata nthawi ndi kuwunikira maselo ojambula.

IphaseImmutherapyRzomasukaPmaufumu

Kuphatikiza pa Lentivirus - Zinthu Zogwirizana Zomwe Zimagwiritsidwa Ntchito Pagalimoto - T kapena Galimoto - NK Zothandiza Zofufuza Zapamwamba, zapanganso Mtsogoleri Wapadera Kuti Athandizire Zatsopano (Cell ndi Minda ina. Iphase yapanga leukocytes ndi ma pbmcs (zotumphukira magazi, kuthandizira kukula kwa immunotherapy.

Zinthu za iphase zimapereka zotsatirazi zabwino zotsatirazi, ndipoSatifiketi Yowunikira (Coa)Kutsimikiziridwa ndi Iphase kumapezeka pa batch iliyonse.

Chitetezo 

Zinthu za Iphase zimachokera kwa opereka opereka ndalama / nyama zomwe zachitika bwino zisanachitike - Kuyang'ana, ndi zotsatira zoyipa kwa ma virus (HIV - 1/2, HCV, Syphilis).

Kukhutisidwa  

Iphase amapereka mafomu ovomereza omwe amasainidwa ndi opereka, omwe ali ndi chitsimikiziro chomveka bwino komanso chowonekera.

Luso

Zopangidwa ndi Iphase, zomwe zimapezeka kudzera ku Leukapheresis, zimathandizidwa ndi akatswiri ophunzitsidwa bwino. Chingwe chilichonse chimatsagana ndi coa yofananira, ndipo zinthu zimayendetsedwa pansi pa unyolo wowongoka kuti mutsimikizire kuti chitetezo, kuperekera mwachangu, komanso chitsimikizo chabwino.

Thandizo lamakasitomala

Iphase akweza kwambiri - Chogulitsa pambuyo - Kugulitsa, onetsetsani kuti zinthu zosasokonekera komanso zosasokoneza.