Kukonzanso PBMC Iyolation: Mapako abwino kwambiri a zotsatira zazitali

Ma cell otuwa a Mononuclear (ma PBMC) amagwiritsidwa ntchito kwambiri pakufufuza zinthu zakale, ndikusewera gawo lofunikira m'minda monga Imnology - Zochitika za Biomerker, Kukula kwa Mankhwala, ndi Celly. Kugwiritsa ntchito kwawo bwino kumadalira njira zolondola zomwe zimasunga khungu la maselo, kuchepa mphamvu, ndi magwiridwe antchito. Kusankha kumanjaPBMC isondikofunikira kuti mupeze - maselo apamwamba oyeserera odalirika.

Kufunikira kwa kukwera - ma pbmcs apamwamba

Ma pbmcs, okhala ndi lymphocytes (ma cell, mahe, ma cell a B, ndi ma cell a NK) ndi monocyte, ndi ofunika kwambiri pakugwiritsa ntchito njira zingapo:

• Galimoto - T Chuma- Chofunika kwa ma cell ang'onoang'ono mu cell ndi njira zochizira.

• Kuleza Mtima- Kuzindikira kuchuluka kwa wodwala komwe kumayankha mosiyanasiyana ndi chithandizo.

• Kupezeka Kwa Biomeker- Kufufuza zolembera zolumikizidwa ndi matenda kapena kugwiritsa ntchito mankhwala osokoneza bongo.

• Maphunziro a toxicology- Kuwona mayankho a chitetezo ku mankhwala opanga mankhwala.

• Kafukufuku wosowa- Kupereka deta yovuta yokhudza mikhalidwe yokhala ndi zitsanzo zochepa.

Popeza chidwi chawo, ma pbmcs ayenera kukhala akutali kugwiritsa ntchito njira zomwe zimachepetsa kuipitsidwa ndikukhala ndi mphamvu kwambiri.

Zosangalatsa PBMC ISO KUGWIRA NTCHITO YABWINO

Pali zinthu zingapo zomwe zingasokoneze kuchira kwa PBMC ndi Kusagwira:

• Kusamalira zitsanzo- Kuchedwa pokonzekera kumatha kubweretsa kuwonongeka kwa cell.

• Njira Yoyeserera- Maluso ngati makonda a centifuation ndi maginito olekanitsidwa amakhudza oyera ndi okolola.

• Ma reagents ndi anticoagulants- mtundu wa zolekanitsa umagwira ntchito yofunika kwambiri posunga umphumphu.

• Kuwongolera kutentha- Kusunga kutentha koyenera pakukonzekera kumalepheretsa kupsinjika kwa maselo ndi apoptosis.

• Kutsatirana ndi GCLP miyezo- Kuwonetsetsa kuti malo a labotale azikhala ndi ntchito zabwino zamalonda zomwe zimapangitsa kudalirika kwa deta.
  
  
  

Kusankha PBMC ISONALLE GET

Mukasankha zida za PBMC isoza, taganizirani izi:

• Kuyera ndi zokolola- Okwera - ma kits olimbitsa thupi amachepetsa kuipitsidwa ndi ma seracytes ndi maselo ofiira a m'magazi.

• Kukonza nthawi- Njira zolerera mwachangu zimathandizira kusungitsa mawonekedwe a maselo.

• Chivinikiro- Ma kits ena amapangidwira zazing'ono - kafukufuku wamkulu, pomwe ena amathandizira okwera - kudzera m'zolowera.

• Kuphatikizika kwamphamvu- Ma PBMC Ayenera kukhala oyenera kugwirira ntchito, yotulutsa cytometry, osakwatiwa - Zingwe za maselo, ndi majini a gene.
  
  
  

Za ifase biosciunces

Kumpoto kwa North Wales, Pennsylvania,Ifase biosciesndi okwera - Exprise Enterprise pofufuza, Kukula, kupanga, ndikugulitsa kwa mitundu yosiyanasiyana yachilengedwe. Ndikudzipereka pakuthandizira asayansi padziko lonse lapansi, gulu lathu la sayansi limapereka - zoyeserera za PBMC ndi njira zina zothandizira popititsa patsogolo kafukufuku wa biomitsa. Iphase amagwiritsa ntchito malo angapo a R & D, malo ogulitsa, ndi malo ogulitsa pa China, United States, Europe, ndi East Asia, kuphimba mikono 12,000.

Ngati mukuyang'ana njira zodalirika za PBMC, timu yathu yakonzeka kuthandiza. Lumikizanani nafe lero kuti muwone momwe ma federan athu amadzipangira amathandizira.