Iphase akabudirira kutanga C57BL / 6 Mouse Primary Hepatocyte

C57BL / 6, kazhinji inotaurwa nezvayo "C57 dema 6", C57 ", kana" dema Iyo inoshandiswa zvakanyanya senzvimbo yekudzvinyirira yekuedza kuongororwa kune kutevedzera vanhu vanoita zvinhu zvekutevedzera. Iyo zvakare imwe yeanonyanya kushandiswa uye yakanyanya kunaka - kutengesa madhiri emakonzo nekuda kwekugona kwayo kuti ishandiswe sedambudziko renyararo, iko kusununguka kwaro kubereka, uye simba rayo.

Ⅰ. Mavambo uye hunhu hweC57bl / 6 makonzo

Muna 1921, C.c. Zvishoma kusungirirwa iyo miss Abby lattrop strain kuti iburitse dzakatetepa dzemhuka; Meted iyo Nha. 57 Mukadzi 57 neNo Nha. 52 Chikadzi Kuti Uwane C57. Akamisa ruvara rwevhudzi rweC57 kusvika kuBrown uye akatumidzwa kuti C57BR (Brown), uye kana gadziridza mhodzi kune dema, inodaidza c57bl (dema). Sezvo paine akawanda mashoma eC57 makonzo, c.c. Little For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For For Furess

Inotariswa se "standard" isina kurohwa

Mukuwedzera, c57bl / 6 inoshanda seyekuedza mhuka yemhando yepamusoro uye yakanyanya kujeka, migumisiro yakafanana, uye kushushikana kwehukama. Semhedzisiro, C57BL / 6 yakava mbeva yekutanga kunetseka kuti ive nerunyararo muBiology Biology, genetics, genetics, photosology, nyota, neurobiology, uye neurology; Izvo zvakare zvakashandiswa kudzidza Senescence, neurodenegenerative Disrict

Ⅱ. Kuenzanisa kweC57BL / 6 Mouse Subtypes

Muna 1947, Jackson Laboratory akasuma C57bl / 6 kubva padiki uye akatumidzwa c57bl / 6j. Muna 1951, National Institute yehutano (NIH) yakaunzwa c57bl / 6j yechizvarwa chezita uye yakaumbwa subline C57bl / 6n kubva kuJackson Laboratory. Sezvo paine misiyano mizhinji pakati pezvikamu, zvinodikanwa kusarudza yakakodzera mbeva yemarara maererano nezvinangwa zvekuedza.

Mukuwedzera, apo C57bl / 6j naC57bl / 6n vakasarudzwa semhando dzemhuka, kwaive nemisiyano mishoma pakati pavo.

Ⅲ. Kukosha kweC57BL / 6 Mouse Primary Hepatocytes

Yokutanga hepatocytes ndeye liver parrenchmar masero zvakananga kubva kune mhuka mhuka, iyo ine zvisinga tsanangurike zvakanakira.

Yokutanga hepatocytes inoenderana neiyo - Vivo nharaunda, uye nzvimbo dzekusangana neyakafanana neyakajairwa. Nenhema, kushandiswa kwePrimary Hepatocytes ye - Vitro Rutsvals haina kukonzera nyaya dzetsika dzekuedza kwemhuka, uye inoderedza mutengo wekuedza kwemhuka. Sezvo ikozvino, yekutanga hepatocytes inoshandiswa zvakanyanya mumanyorero uye cellular biology uye yekudzoreredza bhaibheri rekutsvaga, genomics uye genetics; Panguva imwecheteyo, ivo vanogona kushandiswawo muindasitiri yanhasi yakakurumbira, vakadaro zvinodhaka, kutsvagurudza, kune cancer princency, princarcyte

C57BL / 6 Makonzo, seyakajairika yekuyedza yemhuka yemhuka, ane yakasarudzika yakasarudzika nanhasi mizhinji yemuviri. Naizvozvo, chidzidzo uye kukura kweanonyanya kutaura ndekwekukosha kwakanyanya kwekurapa kwekurapa kwemilidisciplinary, zvirwere zvizhinji zvinodhaka uye kukura kwezvinodhaka!

Ⅳ. Ifase - Zvigadzirwa Zvakabatana

Kupindura izvi, Iphase, semutungamiri wezvivakwa zveBiology for the C57bl

Nyanzvi dzePhasa Vafambi vacho vakashandisa kushamwaridzana kwekupedzisira kwe1 μm velapamil seyenjee substrate, vakasara huwandu hwemushonga pa5 min, 5 min, uye 90 min, uye 90 min, uye 90 min, uye 90 min, uye 90 min, uye 90 min, uye 90 min, uye 90 Min, Ivo vakagumisa kuti hafu - Hupenyu hweVepamail vaive na9.51 min, uye vitro clearance yechiitiko uye vakaburitsa chikamu chakabudirira cheHepatocytes kubva kuC57bl / 6 makonzo!

Kutevedzera

Zvigadzirwa zvePhepase zvinowanikwa kubva mumapamazuva ezvikamu uye zvakajeka zvinoratidzwa.

Chengetedzo

Mhuka dzinoedzwa kune dzinotapukira vamiririri kuti uve nechokwadi chekuchengetedza kwechigadzirwa mhando.

Chiitiko chakakwirira

Nyanzvi dzePhase dzakasimbisa chiitiko cheCytochrome Cyp450 Enzyme uchishandisa Verapamil seSubstrate, uye magadzirirwo akagadzirwa anoratidza iyo inosangana nemutengi wemutengi.

Yakanyanya Kudzorera Rate

Chiyero chekupora cheClypreservation inogona kupfuura 90%.

Inobatsira

Pipaase inogona kupa yakagadzirirwa sevhisi yeakakosha mhando uye matishu zvinoenderana nezvinodiwa nemunhu mumwe nemumwe.

Pamusoro pekubudirira kwekusazviparadzaniswa kweC577bl / 6 Mouse Hepatocytes, Monkey, tangi, tangi, tanga, uye akasimbisa kuti mhedzisiro inosangana nezvinodiwa nevatengi. VaPhepai ​​vanogona kupa vatengi vane akasiyana siyana genera uye madunhu ekutanga hepatocytes kubatsira vatengi nekukura kwezvinodhaka uye miedzo.