NhanganyayaChiropa microsomesuye smo zvidimbu
Mumunda metrabolism metabolism uye pharmacokinetics, vanonzwisisa zvinyoronyoro zvechiropa microsomes uye smo zvidimbu zvakakosha. Izvi muVitro masisitimu anoshandiswa mumatanho ekutanga ekuwanikwa kwezvinodhaka, anopa ruzivo rwekuita kuti zvimisikidzwa zvemakemikari zvitsva (NCES) zvinonzi metabolized neChiropa. Iko kugona kwekufanotaura metabolic kugadzikana uye zvinogona kuitika zvinodhaka zvinodhaka zvakakwanisika, kuchengetedza nguva uye zviwanikwa mukuvandudzwa kwemishonga mitsva yemishonga. Ichi chinyorwa chinodzikira mumusiyano pakati pechiropa chemuchiropa uye zvidimbu zveSCRALDS, kuongorora zvavakaita, kushanda, zvakanakira, uye zvisingakwanisi.Kuumbwa uye chimiro chechiropa microsomes
● Microsomes sechikamu cheExplasmic Reticulum
Mufananidzo wechido uri vesicle - kunge masicts inotorwa kubva ku endyoplasmic reticulum muEukaryotic masero. Ivo vakaumbwa kana masero aputswa panguva yekuita kweHomogenizization, iyo inoguma nekuita kweiyo yekudzokorora kwe endyoplasmic reticulum. Ichi chidimbu chidimbu chinopfumiswa nemambrane - yakasungwa enzymes uye iri parmount mukudzidza zvinodhaka metabolism, kunyanya chikamu chandinoita.● Key enzymes inobatanidzwa: Cytochrome P450s uye maatts
Iyo yakanyanya kukwira cytochrome P450 enzymes (coms) muchiropa mikrorrosomes ndiko kutsanangura kwavo. Aya enzymes anotamba basa rakakosha mune yehusiku metauro yehukuru hwe xenobiotic, kusanganisira zvinodhaka uye zvakatipoteredza. Zvakare zviripo uri Uridine diphosphate - Gluculonosyltransfansfansfansfansfansfansfansfanes (iyo inobatsira kuseverana uye kubvisa danho rekutanga renzwisiso yeNzwi Ini neChikamu II uye chimwe chikamu chechipiri.Kuumbwa uye chimiro cheS9 zvidimbu
● S9 semubatanidzwa weMicrosomal uye Cytosolic Zvidimbu
Chikamu cheS9 Kusiyana microsoes ine predominy fungidziro retolum Retolum● Enzymes aripo muS9 inosanganisira chikamu ini naI II
Zvidimbu zveSCHASHITION zvinopa enzymatic yemhando yepamusoro Urongwa uhwu hunobvumira kune zvimwe zvakawedzera metabolic shanduko, simudzira chiropa chiri muVivo nharaunda zvakanyatsoitika pane microsomes ega.Kushanda: Chikamu ini uye chikamu Ii metabolism
● Kusiyana muEzzyme chiitiko pakati pemicrosomes uye s9
Musiyano musiyano unorara muEnzymatic Scope. Mufananidzo wechido rwechiropa zvakanyanya kutsigira chikamu chandinoridza metabolic, chinosanganisira oxidation, kuderedza, uye hydrolysis. Aya maitiro anounza kana kufumura mapoka anoshanda pane mhezi dzemidhara, achivhura nzira yehase yechipiri. Mukupesana, SLSATIONS inosanganisira zvikamu zviviri izvi ini na II, nekudaro tichifambisa musangano unoita kuti micrurosomes haigoni kuzvimiririra.● Kukosha kwechikamu i naI mune zvinodhaka metabolism
Iyo inotevedzera chimiro che chikamu ini na ii maitiro anosimbisa kukosha kwavo mune zvinodhaka. Chikamu chandinogadzirisa chinowanzo gadzirira makomputa e phase ii, iyo inowanzo kusanganisira kuvanzika kuti iwedzere kuwedzerwa solubility, kubatsira mune maitiro ekuratidzira. Kunzwisisa zvikamu izvi zvakakosha kuti ufanane neanodhaka bioavilabolability, kushanda, uye kugona kuita.Zvishandiso muzvinodhaka metabolism zvidzidzo
● Shandisa Adme Zvidzidzo: Kupinda, Kugoverwa, metabolism, kubviswa
Mufananidzo wechipenga uye s9 zvidimbu zvinopindirwa muAdme Zvidzidzo, izvo zvinoongorora iyo yekukanganisa, kugoverwa, metabolism, uye kubvisa kwezvinodhaka. Izvi zvidzidzo zvinokosheswa mukusarudza iyo pharmacokinetic profile yemishonga, kupa yakakosha data iyo inozivisa doses, inogona kutaurirana, uye mhedzisiro.● Kuenzanisa Zvidzidzo: Microsomes vs. S9 Mukuwanikwa kwezvinodhaka
Kuongorora kuongorora pakati pemicrosomes uye smo zvidimbu zvinoratidza kuti ivo vaviri vachishandisa zvavakaita, SLSATIONS inopa yakawanda maonero e metabolic kugadzikana nekuda kwekusanganisira chikamu cheII enzymes. Aya ekunyatsoongorora ACKSSIVE AIDS mukuzivikanwa kweMetabolic nzira idzo dzingave dzisingaonekwe kana uchishandisa chiropa microsomes ega.Zvakanakira zvechiropa microsomes
● Mutengo - Kubudirira uye Kuwanika
Chiropa microsomes zvinofarirwa nemutengo wavo - Kubudirira uye Kuwanika Kwazvo. Maitiro avo ekugadzirira kwavo uye kushandiswa kwakasimba - yakasimbiswa, kuvaita sarudzo inoshanda kune vazhinji vemabaratori.● Yakakwira Kupfuura uye AutoMuation Kuenderana
Iko kusarongeka kwezvinhu zvinokanganisa zvinoita kuti vasimudzire kusvika pakakwirira uye automation, chinhu chakakosha chekuongororai mararaii emaraibhurari nekukurumidza. Iyi mukana yakakosha pakuratidzira iyo pombi yezvinodhaka, ichibvumira kuzivikanwa kwekutanga kwekuvimbisa vanokwikwidza.Zvakanakira zvechiropa S9 zvidimbu
● Yakanyudzwa metabolic inomiririra
Nechikamu chose ini naI enzymes, s9 zvidimbu zvinopa yakawanda yekuongorora yakazara yeiyo metabolund ye metabolic. Ichi chinomiririra chakazara chinonyanya kubatsira pakuzivisa zvinogona kuitika metabolites pakutanga mune zvinodhaka maitiro.● Kubatanidzwa kweChikamu II metabolism uye mutengo kufunga
Kusanganisira chikamui II enzymes zvinoreva kuti ma lracsions iyoyo anogona kupa nzwisiso mukuita zvinoenderana nekubatana, kufanotaura kuti zvinodhaka zvichagadziriswa mumuviri. Nepo ivo vangangoda mamwe macficor kune zvimwe zvekuita, ivo vachiri kupa zvakanaka mwero wemutengo uye zvinoshandiswa munzvimbo dzakakwirira yekupfuura.Kugumira kweropa microsomes
● Kusavapo kweiyo Cytosolic enzymes inokanganisa yakazara metabolism data
Kuganhurirwa kwakakosha kwechiropa mikrorrosomes ndiko kushomeka kwavo kwecytosolic enzymes, izvo zvakakosha kuti izere metabolic mufananidzo. Uku kusavapo kunoreva kuti mamwe metabolites anogona kunge akafuratirwa, kushaya nzira dzakashata dzakakosha kuti unzwisise kuchengeteka kwezvinodhaka uye kushanda.● Zvinodiwa kune mamwe masisitimu ePhase II kudzidza
Kuti udzidze chikamu chechipiri Ichi chinodiwa chinogona kuomesa girowa uye kududzira.Kuganhurwa kweRopa SCHALS
● Kuda CO - Zvezvinhu uye zvinokwanisika enzyme dilution
Pasinei nechimiro chavo chakazara, zvidimbu zveS9 zvinowanzoda kuwedzera kwezve cofecors kutsigira mamwe mabasa enzymatic. Izvi zvinodiwa zvinogona kusuma kusiyana uye kuoma kuita masaga. Pamusoro pezvo, kudhirowa kweEnzymes muScracsions, kuenzaniswa kugadzirira kuri kwega, kunogona kukanganisa kunzwa kwekuona kwekuonekwa kwemamwe metabolic.● Kuchengetedza uye Kugadzirira Matambudziko Kuenzaniswa neMicrosomes
Kugadzirira uye kuchengetwa kwezvidimbu zvidimbu zvidimbu zvinogona kuve zvakaoma kupfuura microurrosomes. Kugadziriswa kwakakodzera kweEnzymatic chiitiko kuburikidza nekuchenjera kubata uye kuchengetedza kwakakosha kuwana mhedzisiro yakavimbika, kuomesa zvishoma kushandiswa kwavo mune hombe - zviyero zvidzidzo.Mhedziso uye Kurudziro
● Pfupiso yeakanakisa - Shandisa mamiriro ezve imwe neimwe nzira
Chiropa microsoms uye s9 zvidimbu chimwe nechimwe chine masimba akasiyana uye kushaya simba. Mufananidzo wechiLiver microsomes anoshandiswa zvakanyanya kana chiripo Mukupesana, SLCALICES IZVO ZVINOGONESESWA NEZVINHU ZVINOGONESESA ZVINOGONESESA ZVINOGONESESA ZVINOGONESESESA ZVINOGONESA ZVESE ZVESE ZVESE ILI NO II Metabolism. Kusarudza iyo yakakodzera hurongwa zvinoenderana nezvinodiwa zvekutsvaga uye zviwanikwa zviripo.● Mazano ekushandisa kwakakwana mukubudirira zvinodhaka maitiro
Kune avo vari mumatanho ekutanga zvinodhaka zvinodhaka, vanoshandisa kukwirira - mhando yemhando yemifananidzo kubva kune inozivikanwa chiropa microsomes kana chiropa microsomes inogona kuongorora mari yekutanga. Sekutsvagisa kunofambira mberi, kusanganisachiropa s9Zvidimbuzve, zvimwe zvinokonzerwa nemuchato wakavimbika microsomes fekitori, ichapa mufananidzo wakazara wekugadzikana kwejabolic, uchiita kuti imba yakasimba inokwezva mishonga.● NhanganyayaIfase
Musoro wakadzika muNorth Wales, Pennsylvania, Pefese Boscience is "nyanzvi ye" nyanzvi, uye inokwirisa " Kuzvidza ruzivo rwakakura uye kusuwa kwesainzi Kutsvaga iyo R & D yakakodzera ye "Dzinza dzinza, ichitsvaga ramangwana," Iphase yakatanga akawanda r & d zvivakwa, nzvimbo dzekutengesa, dzimba dzekutengesa, dzimba dzekuzvidzivirira pasi rose.
Post Nguva: 2024 - 11 - 26 - 26: 5:50:02