Iphayan da aka samu samu an ƙaddamar da c57bl / 6 na firayim

C57BL / 6, sau da yawa ana kiranta "C57 Black 6", "C57", ko "Black 6" (mizani na yau da kullun B6), abin ƙyama ne na linzamin dakin gwaje-gwaje. Ana amfani dashi azaman linzamin kwamfuta na transgenic a cikin gwajin kwayar halittu zuwa lahani na halittar mutum. Hakanan yana daya daga cikin yalwar da aka fi amfani da shi kuma mafi kyau - Siyar da iri na mice saboda iyawar da za a yi amfani da shi azaman tsinkayar haihuwa, da kuma ƙarfinsa.

Ⅰ. Asali da halayen C57BL / 6 mice

A cikin 1921, c.c. Kadan inbred da Miss Abby Lathrop don samar da baƙin ciki da yawa; Mury No. 57 mace tare da No. 52 namiji don samun C57. Ya tsayar da launi na murfin c57 zuwa launin ruwan kasa da kuma sanya shi C57br (launin ruwan kasa), kuma lokacin da aka gyara Jawo, wanda aka kira shi C57BL (baki). Kamar yadda akwai manyan tallace-tallace na C57 mice, c.c. Kadan ƙara zama layi 6 a cikin 1937, ya sanya masa alama C57BL / 6.

An ɗauke shi azaman "daidaitaccen" iri, da C57BL / 6 yana samar da asalin halittar halittar da yawa daga cikin halittar halaye da yawa.

Bugu da kari, C57BL / 6 tana da tsarin gwaji na gwaji tare da madaidaici, sakamako mai mahimmanci, da kuma amsawa mai ban sha'awa. A sakamakon haka, C57BL / 6 ya zama farkon mouse don samun bincike na asali, ilimin halittar asali, ilimin halittar jiki, ilimin halittar jiki, da neuriyanci, da neurology, da neurology, da neurology, da neurology, da neurology, da neurology, da neurology, da kumaurology; Hakanan an yi amfani da shi don nazarin abubuwan warwarewa, cututtukan ƙwayar cuta (misali cutar Alzheimer), rashin haƙuri, rashin haƙuri da cututtukan ƙwayar cuta.

Ⅱ. Kwatanta C57BL / 6 Motttypes

A cikin 1947, Jackson dakin gwaje-gwaje na C57BL / 6 daga kadan kuma sunanta shi C57BL / 6J. A cikin 1951, Cibiyar Kiwon Lafiya (NIH) ta gabatar da C57BL / 6J 3-37ND GWAMNATIN DA SIFFOFI Subline C57BL / 6n daga dakin gwaje-gwaje Jackson. Kamar yadda akwai bambance-bambance da yawa tsakanin sublanes, ya zama dole a zaɓi yanayin linzamin kwamfuta da ya dace bisa ga dalilai na gwaji.

Bugu da kari, lokacin da C57BL / 6J da C57BL / 6N aka zaɓi kamar yadda aka gwada ƙirar dabba, akwai ƙananan bambance-bambance tsakanin su.

Ⅲ. Mahimmancin C57BL / 6 Mouse Farko Heckatocytes

Primine hepatocytes ne sel sel na hanta kai tsaye ware daga mahayan dabbobi, waɗanda basu da fa'ida ga masu ba da izini ba.

Fim defatocytes sun yi daidai da A - yanayin VIVO, da kuma cofactors bincike game da buƙatun na al'ada na al'ada. A lokaci guda, amfani da farko hepatocytes na a - Binciken Bincike ba ya haifar da abubuwan da dabbobi dabbobi, kuma yana rage farashin gwaje-gwajen dabbobi. Kamar yadda, an yi amfani da farko na hepatocytes da yawa a cikin kwayoyin halitta da kuma bincike na asali, kamar kariya, ganyen jiki da kwararru; A lokaci guda, ana iya amfani dasu a cikin sananniyar masana'antar tsirara ta yau, kamar bincike na magunguna, bincike mai lalacewa, da sauransu a zahiri, da sauransu hepatocyte yana da mai ba da izini, da sauransu kyakkyawan yanayi.

C57BL / 6 mice, a matsayin wani samfurin dabbobi na gwaji, mallaki na musamman amma yana da amfani kaddarorin ilimin kula da juna. Saboda haka, nazarin da ci gaban farko hepatocytes ne m mahimmancin ci gaba da lura da multifidist mai yawa, cututtukan da yawa na da yawa da ci gaban kwayoyi!

Ⅳ. Iphayan

Da yake amsa wannan yanayin, Iphayan, a matsayin jagora a cikin realents na reacat-/ 6 ya sami nasarar samar da kayan aiki tare da sabon zabi don ci gaban magunguna da sauran gwaje-gwaje!

Masu fasaha na Iphayan sun shiga dakatar da dakatarwar hepatocyte na farko daga gauraye Male C57BL / 6 Mice da kuma gwada aikin dakatarwar magani tare da maganin karewa. Masu fasaha sun yi amfani da maida hankali na tsawon shekaru 1 a matsayin ingantaccen sigari, mintuna 5, 15 min min, 20 mintuna, da 90 min, kuma an gano cewa maida hankali ne a hankali. Sun ƙarasa da cewa rabin - Rayuwar Verapamil ya kasance minti 28.51, da sel na ciki shine 0.0486 ML 2.0486 ml / sel miliyan, 6 mice!

Yarda

Ana samun samfuran samfuran Iphayan daga tushen hukuma kuma an gano su a sarari.

Tsaro

An gwada dabbobi don masu kamuwa da kamuwa don tabbatar da amincin ingancin samfurin.

Babban aiki

Masu fasaha na Iphayan sun tabbatar da ayyukan cypyme cyp450 enzyme ta amfani da verapamil azaman substrate, kuma samar da sakamako wanda ya hadu da matsayin abokin ciniki.

Babban sakamako

Sakamakon dawo da kukan crypreation na iya wuce 90%.

M

Iphayan na iya samar da sabis na musamman don nau'in nau'in musamman da kyallen takarda bisa ga bukatun abokin ciniki.

Baya ga sabon abu na cin nasarar da aka dakatar C57BL / 6 Hepatocytes na yau da kullun da gwajin enzite sun kuma tabbatar da cewa sakamakon ya cika bukatun abokan ciniki. Iphayan zai iya ba wa abokan ciniki tare da nau'ikan Genera da bayanai game da hepatocytes na farko don taimakawa abokan ciniki da ci gaban kwayoyi.