Sakamakon gwaji mara gamsarwa? Sabuwar sigar in - vitro micronucleus gwajin kit yana nan don taimakawa!

KASHI NA 01. A - Gwajin Micronucleus na Vitro

Micronuclei duka chromatids ne ko gutsuttsura acentric ko chromosomes na zobe waɗanda ke zama a cikin cytoplasm lokacin da chromosomes akai-akai suna shiga sel 'ya'ya don samar da nuclei bayan mitosis. Domin wadannan gutsuttsura ko chromosomes ba za su iya shiga babban tsakiya a telophase ba, lokacin da 'ya'yan 'ya'ya suka shiga tsaka-tsakin gaba na gaba, sai su taru zuwa kananan ƙananan tsakiya a waje da babban tsakiya kuma su zama micronuclei. Tare da haɓakar tsarin haɓakawa, mahimmancin micronuclei, ci gaba da inganta hanyar ganowa da kamala na dabarun gwaji, gwajin micronucleus ya zama ɗaya daga cikin manyan hanyoyin don tantance maye gurbi. Ana amfani dashi ko'ina don tsinkayar haɗarin cutar kansa, gano masu haifar da aneuploidy da sauran haɗarin kwayoyin halitta.

Gwajin in - vitro micronucleus hanya ce ta gwajin genotoxicity don gano samar da micronuclei a cikin sel masu shayarwa bayan an yi musu magani da kayan gwaji. Yana da manufa don duba karyewar chromosome da aneuploidy da aka jawo a cikin ƙwayoyin mitotic yayin/bayan fallasa ga abin gwajin. Idan aka kwatanta da gwajin in-vivo, gwajin in-vitro micronucleus gwajin ya fi sauƙi, sauri, kuma mai rahusa. Bugu da ƙari, ba ya shafar abubuwa kamar bambancin mutum tsakanin dabbobin gwaji. Tunda gwajin in - vitro micronucleus gwajin zai iya gano halayen micronucleus da wasu sinadarai suka jawo a ƙananan taro tare da babban hankali. Don haka, an yi amfani da shi sosai a fagen ilimin ƙwayoyin cuta.


KASHI NA 02. In vitro micronucleus test kit

Kayan gwajin IPHASE in-vitro micronucleus gwajin yana amfani da sel Hamster Lung (CHL) na kasar Sin azaman tsarin gwaji. Kwayoyin CHL an fallasa su zuwa abu na gwaji tare da / ba tare da tsarin kunnawa na rayuwa ba kuma ana bi da su tare da actin polymerization inhibitor cytochalasin B. An ba da damar kwayoyin su isa ga mitar su na kwayoyin binucleated, sa'an nan kuma girbe, shirya, da tabo; sannan ana nazarin adadin ƙananan ƙwayoyin sel waɗanda suka kammala mitosis guda ɗaya (kwayoyin da aka yi binucleated) a ƙarƙashin na'urar hangen nesa don kimanta yiwuwar mutagenic na abin gwajin. Kit ɗin IPHASE yana ba da duk mahimman reagents da sel don gwajin in - vitro micronucleus. Tun da duk kayan aikin sun ƙetare ƙaƙƙarfan gwajin inganci, sakamakon gwajin da aka samar daidai ne, abin dogaro kuma ana iya sakewa.

--FALALAR KYAUTATA --

1. saukaka

Yana adana lokaci don jawo S9 da shirye-shiryen reagent,. Ana iya amfani da kit ɗin kai tsaye, yana rage yawan sake zagayowar gwaji.

2. Daidaito

Kowane bangare na reagent kit an yi shi da tsauraran gwajin inganci, don haka sakamakon gwajin da aka yi amfani da kit ɗin daidai ne, abin dogaro kuma ana iya sakewa sosai.

3. Kwanciyar hankali

Kit ɗin yana da ƙarfi kuma mai sauƙin ɗauka da adanawa.

4. Yawanci

Aikace-aikace da yawa, ana iya amfani da su don gwajin ƙwayoyin cuta na abinci, sinadarai, magungunan kashe qwari, magungunan kashe qwari, kayan abinci, kayan kwalliya, da sauran su.

Kwatanta sabbin kayan aiki da tsofaffi

IPHASE yana bin ƙa'idodi na ƙasa da jagororin in- gwaje-gwajen micronucleus a fagage daban-daban azaman jagorar fasaha. Bugu da ƙari, IPHASE yana ɗaukar ra'ayoyin abokin ciniki a matsayin manufar ingantawa don daidaita abubuwan da ke tattare da samfurin kuma ya sami nasarar haɓaka kayan gwajin in-vitro micronucleus don saduwa da ƙarin buƙatun abokin ciniki koda bayan ci gaba da tabbatarwa.

1. Haɗin Samfur

Giemsa tabo reagents da reagents da ake buƙata don tsarin shiri an haɗa su don sa ya fi dacewa da inganci.

2. Hanya

Ka'idar kit ɗin ta fi dalla-dalla, tare da haɗa matakan kariya na gwaji da sauran abubuwan ciki don guje wa kura-kurai na gama gari.

KASHI NA 03. Samfura masu dangantaka