Gabatarwa zuwaHanta micosomesda kuma s9
A fagen magunguna miyagun ƙwayoyi da magunguna, fahimtar abubuwan da ke cikin cututtukan hanta da cromptionsari na S9 yana da mahimmanci. Wadannan a cikin tsarin vitro suna da mahimmanci a farkon matakan binciken magunguna, samar da fahimta cikin yadda sabbin abubuwa ke sinadarai (NCES) da hanta yake metabolized ta hanta. Ikon hango maƙasudi mai mahimmanci da kuma rikicin miyagun ƙwayoyi shine mahimmanci, ceton lokaci da albarkatu da albarkatu a cikin ci gaban manyan magunguna. Wannan labarin ya cancanci cikin bambance-bambance tsakanin microomes da kuma kashi na S9, bincika abubuwan da aka sanya, aiki, fa'idodi, da iyakoki.Abun da kuma tsarin hanta microomes
● Microsomes a matsayin wani ɓangare na nazarin gwaji
Halinsa Microsomes sune Vesicle - Kamar kayayyaki iri iri da aka samo daga wasan kwaikwayon na entoplasmic a cikin sel Eukaryotic. An kafa su ne lokacin da aka rushe sel a lokacin aiwatar da tsarin homogenization, wanda ke haifar da ƙwayar ƙwayar gwajin gabaɗaya. Wannan subcellular yanki yana wadatar da membrane - enzymes daure enabzolmes kuma yana da ma'ana wajen yin nazarin metrism miyagun ƙwayoyi, musamman lokaci na hali.● Key enzymes da ya shafi: cytochrrome p450s da cinya
Babban taro na cytochrrome P450 enzymes (cyps) cikin hanta Microsomes shine fasalin su. Wadannan enzymes suna taka muhimmiyar rawa a cikin kerbolatism na kasusuwa na wasu kewayun Xenobiotiks, ciki har da kwayoyi da kuma gubobi da kuma gubobi da kuma gubobi da kuma gubobi na muhalli. Hakanan gabatar da manoma nexhosphate - glucuronosyltransferfesfores (ƙugiya), wanda ke ba da gudummawa ga conjakcrites da kawar da matakin metabolites da kuma kawar da mataki na fahimtar I da wasu halayen na II da wani lokaci na ii.Abincin da tsarin S9
● S9 a matsayin haɗuwa da microsomal da cytosolic
Kashi na S9, sau da yawa ana magana da shi azaman mai sawa 9000g, shine kayan aikin da aka samo daga hanjin homogenates ta hanyar banbanci centriquit. Ba kamar Microsomes da ke dauke da gyaran tarihi ba - a ɗaure enzymes, suwan s9 sun hada da duka enzymates na enzymat.Enzymes suna nan a cikin S9 ciki har da lokaci ni da II
Kashi s9 yana samar da miliymic milieu, in ji shi da lokaci-lokaci i enzymes kamar cyps, da sufurnosfereslasfesfesfesfesfesfesfesfesfesfesfesfesfesfesfesfesedlases, kamar sulutonossferesforts, da kuma glutonovere - siyarwa. Wannan tsararren yana ba da damar ƙarin sauyin yanayi na rayuwa, simulating na hanta a cikin yanayin vivo ya fi dacewa da microsomes shi kaɗai.Aiki: Lokaci I da Lokaci Na Lokaci II Metabolism
Bambanci a cikin aikin enzyme tsakanin microsomes da S9
Cibiyar bambance-bambancen da aka ta'addanci a cikin ikon enzymatic. Hani Microsomes da farko suna goyon bayan lokaci na rayuwa na rayuwa, ya shafi hadawa, ragewa, da hydrolysis. Wadannan halayen suna gabatar da kungiyoyin masu aiki akan kwayoyin ƙwayoyin magani, suna tsara hanyar don yawan lokaci na II. A bambanta, s9 ctionsctions kewaye da biyu maganganun da ni da II, ta haka ne ta yi amfani da juna game da Microsomes ba a yi ba da izinin yin hakan.● Tabbatar da lokaci ni da II cikin metabolism magunguna
Matsayin da aka yi na lokaci-lokaci ni da II na II sun ba su mahimmanci a cikin metabolism magani. Lokaci na gyare-gyare-gyare-gyare ne na shirye-shiryen shirya mahimman lokaci na II, wanda yawanci ya shafi jituwa don karuwa da karuwa, taimako a cikin tsari na excretion. Fahimtar waɗannan matakai suna da mahimmanci don tsinkaya irin magani, inganci, da kuma masu wahala.Aikace-aikace A A cikin Karatun Metabolism
● Yi amfani da karatun Adme: Shefe, Rarrabawa, Metabolism, kawar
Kasar Microsomes da Kayayyakin S9 suna da matakai a cikin karatun Admees, wanda ke kimanta sha, rarraba, da kuma kawar da 'yan takarar miyagun kwayoyi. Wadannan karatun suna da mahimmanci wajen tantance bayanan magunguna na magunguna, samar da mahimman bayanai waɗanda ke sanar da Dosing, hulɗa da juna.Nazari na kwatanci: Microses vs. S9 a cikin binciken magunguna
Nazarin kwatankwacin ajizai tsakanin microsomes da Fassara suna bayyana cewa yayin da Dukansu suna amfani da su, juzu'i na S9 suna ba da cikakkiyar hangen nesa na rayuwa saboda haɗa enzymes. Wannan cikakken bincike Awarewa a cikin tantance hanyoyin hanyoyin da bazai bayyana lokacin da amfani da microcomes hanta.Abvantbuwan amfãni na microomes
Kudinsa - tasiri da samarwa
Halin da Microsomes sun fifita farashinsu - tasiri da yawan shakatawa. Hanyoyin aiwatar da shirye-shiryen su da amfani dasu suna lafiya - an tabbatar da su, suna sa su zabi mai amfani ga dakunan gwaje-gwaje da yawa.● Babban Hanya da Karancin Automation
Sauƙin Lissafin Microsome yana sa su ƙi zuwa Hanya ta Hanya da Automation don kula da manyan ɗakunan karatu na mahadi cikin sauri. Wannan fa'idar tana da mahimmanci a cikin matattarar kwayar cutar ta PIPELINCE, ta ba da izinin gano farkon takardu.Abvantbuwan amfãni na hanta s9
Ingantacciyar wakilci na ƙarshe
Tare da duka lokaci-lokaci I da II enzymes, s9 Forcess suna ba da cikakken cikakken kimantawa na tsarin rayuwa na yau da kullun. Wannan irin wakilci yana da amfani musamman gano yiwuwar metabolites da yawa a cikin tsarin cigaban miyagun ƙwayoyi.● Haɗu da lokaci na ii metabolism da la'akari mai tsada
Ciki har da lokaci na II Enzymes yana nufin cewa S9 ctionsictions na iya samar da alsila cikin ayyukan haɗin gwiwa, tsinkayar yadda za a sarrafa magunguna a jiki. Yayin da zasu iya buƙatar ƙarin courcors ga wasu halayen, har yanzu suna ba da daidaitaccen farashi da amfani a cikin saitunan isasshen saiti.Iyakancewar hanta microomes
Rashin Ingancin Cytoscol ya shafi cikakken bayanan metabolism
Muhimmin iyaka na Microsomes na hanta shine rashin enzymes na Cytoscol, waɗanda suke da mahimmanci don cikakken hoto na yau da kullun. Wannan rashi na nufin cewa wasu hanyoyin metabolites za a iya watsi da su, sun rasa hanyoyin hanyoyin muhimmiyar hanya don fahimtar amincin ƙwayoyi da inganci.● Bukatar buƙata don ƙarin tsarin Nazari na II
Don yin nazarin lokaci II reactions sosai, ƙarin ƙarin tsari ko ƙarin enzymes / cockactors ya zama dole lokacin amfani da microsomes. Wannan buƙatun zai iya magance ƙirar assay da fassarar.Iyakokin hanta s9
● Bukatar don CO - Abubuwa kuma tabbatattun enzyme
Duk da cikakkiyar dabi'a, gutsattsarin S9 sau da yawa suna buƙatar ƙari na cofactors don tallafa wa wasu ayyukan enzymat. Wannan bukatar na iya gabatar da bambance-bambance da rikitarwa cikin magudin. Ari ga haka, da duffuwa da enzymes a cikin s9, idan aka kwatanta da shirye-shiryen ware, idan aka shirya da abubuwan da ganowa na wasu halayen wasu halayen da suka faru.Adana da kuma shirye-shiryen shirye-shirye idan aka kwatanta da microsomes
Shiri da kuma adana s9 na iya zama mafi kalubale fiye da sabili da Microsoomes. Daidai ne na aikin enzymatic ta hanyar kulawa mai kulawa da ajiya yana da mahimmanci don samun sakamako mai aminci, dan kadan rikit rikicewar su a manyan - Prale nazarin su.Kammalawa da shawarwari
● Takaitaccen bayani - Yi amfani da yanayin kowane hanyar
Kashi Microses da Fasaha na S9 kowannensu yana da ƙarfi daban-daban da rauni. An yi amfani da Microsomes mafi kyau lokacin da farashi - Inganci, saurin lokaci i yana buƙatar allon yanar gizo. A bambanta, s9 ctionitalungiyoyi sun fi dacewa da cikakkar karatu da bukatar fahimta cikin lokaci na i da II metabolism. Zabi tsarin da ya dace ya dogara da takamaiman buƙatun bincike da kuma albarkatun da ake samu.● Shawarwari don ingantaccen amfani a cikin ayyukan cigaban miyagun ƙwayoyi
Ga wadanda ke cikin matakan ci gaban magani, suna da girma sosai - Micrikes ingancin hanta Microsomes daga mai siyarwar Microsomes za ta iya jera. Kamar yadda bincike yake bincike, hada shihanta s9Kashi, wataƙila ya fisshe daga masana'antar hanta mai aminci, zai ba da cikakkiyar hoto na ƙirar metabolic, don tabbatar da kimantawar ɗan takarar ƙwayoyi.Taggara zuwaIphayan
Headquartered in North Wales, Pennsylvania, IPHASE Biosciences is a "specialized, novel, and innovative" high-tech enterprise integrating research, development, production, sales, and technical services of innovative biological reagents. Levorging mai mahimmanci da sha'awar kimiyya, ƙungiyar kimiyya ta himmatu wajen samar da ingantacciyar hanyar kirkirar halittu a duk kokarin masu karatunsu don taimakawa cimma manufar binciken su. Finawa da R & D mafi kyawun nazarin, reagenting na gaba, "Iphates ya kafa makomar gaba," cibiyoyin tallace-tallace, shagunan tallace-tallace, da rarraba kayan aiki a duk faɗin duniya.
Lokaci: 2024 - 26 16:50:59