Yadda ake shirya nunin faifai don Assay
DaAsus Assay, kuma da aka sani da guda - Cell Electrophoresesis, wata dabara ce mai mahimmanci ga ƙimar darajar DNA a cikin sel mutum. Shirya sosai - inganciOmet asay zamewas mataki ne mai mahimmanci wanda ke ƙayyade amincin da kuma sake haifuwa game da sakamakon assay. Wannan labarin na nufin samar da cikakkiyar jagora a kan shirya nunin faifai don undet assay, hanyoyin fahimta daga masana a fagen. Za mu rufe kowane muhimmi mataki, daga zaɓi zaɓi zuwa fassarar bayanai, tabbatar muku samun kyakkyawan sakamako mai yiwuwa.Gabatarwa zuwa Assay Assay da mahimmancinsa
● Takaitaccen Bayani na Assayi
Taron Assayi, wanda ya ci gaba a ƙarshen 1980s, kayan aiki ne mai ƙarfi don gano ɓarke DNA Strand a cikin sel Eokinyotic. Ana amfani dashi sosai a cikin filayen kamar gwajin Genotoxicity, masifirta kwayoyin, da kuma bitororin muhalli. Ka'idar Assay ta hada da sel ta hada sel a cikin Agaris, karya su don cire membranes da sunadarai, sannan kuma batun DNA zuwa electrophoresis. A sakamakon image, kama da wani tauraro, yana ba da damar faɗakarwar lalacewar DNA.● Aikace-aikace a Ganuwar DNA
Ambet Asays suna da matukar mahimmanci a gano lalacewar DNA wanda aka haifar ta hanyar zumunta muhalli, radiation, radiation, radiation Suna kuma taka muhimmiyar rawa wajen kimanta ƙarfin DNA a cikin hanyoyin gyara DNA a nau'ikan sel daban-daban. Masu bincike suna amfani da amintattun 'yansuwa don nazarin cutar kansa, tsufa, da sauran cututtuka da ke da alaƙa da lalacewa ta DNA.● mahimmanci a cikin gwajin Genotoxicity
Tsarin tsari da mahimmancin bincike na kayan kwalliya ba za a iya fahimta ba. Suna aiki a matsayin muhimmin kayan aiki na PV na PV nada don gano makamashi mai zurfi. Sakamakon wannan sakamakon na iya tasiri shawarar shawara - Yin matakai a cikin ci gaban magunguna da kimantawa na kare muhalli.Zabi na kwayoyin da suka dace don Assay Assay
● nau'ikan ƙwayoyin sel da aka yi amfani da su
Zabi sel na iya haifar da sakamako mai mahimmanci a assay. Kwayoyin da aka saba amfani da su sun hada da lymphocytes, sel na kwastomomi, da layin tantanin halitta daban-daban. Masu bincike sukan zabi nau'ikan sel dangane da manufofin nazarin da sauƙin samun samfurori.● la'akari don zabin tantanin halitta
Lokacin zaɓar sel, yi la'akari da dalilai kamar abubuwan da ake samu sel, ƙwaƙwalwar ƙwaƙwalwar ƙwaƙwalwa, da kuma dacewa ga tambayar bincike. Misali, lymphocytes ana samun sauƙin jini daga jini kuma suna kula da wakilan mutanen Genotoxic, suna yin su kyakkyawan zabi don karatu da yawa.Tasiri kan sakamakon assay
Asalin da jihar sel na iya shafar matakin tushe na lalacewar DNA da kuma martani na tantanin halitta. Saboda haka, daidaitaccen zaɓi na tantaninci da kuma ma'amala da ladabi yana da mahimmanci don samun sakamako amintaccen kuma haifuwa sakamakon amsawa.Shirya Gels Aikin
● Nau'in Agarose don Amfani
Zabi da madaidaiciyar agarosa yana da mahimmanci don amincin Assay. Lowing melting point an fi son shi saboda yana ƙarfafa lalacewa da sauri kuma yana rage lalacewa DNA yayin aiki. High - Ingancin Agarose daga Masu ba da izini, kamar waɗanda daga amintaccen Auren Assay slide mai ba da kaya ko Comet Assay slideTasirin Aikin Aikin Aikin
Yawanci, 1% ƙananan ƙananan melting Point ana amfani da taro. Narke da agaroosa a cikin buffer mai dacewa, yana shayar shi har sai an narke mai kyau sosai, sannan sanyi zuwa zazzabi wanda ba shi da haɗari ga ɗakin tantancewa. Tabbatar da agarosa kyauta ne daga kumfa da tarkace don kauce wa kayan tarihi a cikin assay.● tabbatar da ingancin gel
Ingancin gel din agel na iya yin tasiri a hankali assay da haifuwa. Hanyoyin sarrafawa da kyau da shirye-shiryen fasahohi suna da mahimmanci don samar da gel mai daidaituwa tare da daidaitattun kaddarorin a fadin tsoka daban daban. Wannan yana tabbatar da cewa samuwar comets da gani suna da kyau.Dakatarwar sel da kuma fitar da sel a Agarose
● Shirya dakatarwar sel
Fara ta hanyar samun guda - dakatarwar sel. Don sel na gargajiya, wannan ya shafi ƙoƙarin yin ƙoƙari, wanda aka bi da shi da wanka. Ga sel na farko kamar lymphocytes, centrifugation da sake faduwa a cikin buffer mai dacewa wajibi ne. Yi amfani da hemocytometer don tabbatar da taro na tantanin halitta kimanin 1 x 10 ^ 5 sel / ml.● Emfeded sel a cikin ƙananan narkewa
Mix celarwar sel tare da pre - warmed melting maki matsa a wani rabo na aiki wanda ke tabbatar da daidaitattun rarraba sel. Da sauri bututun cakuda a kan pre - mai rufi undy nunin faifai, wanda za a iya siye daga masana'anta mai ban sha'awa ko mai samar da kaya. Yi amfani da Coverslip don yada agaroo a hankali da hana kumburin iska.● Elderwallon sel a kan nunin faifai
Da zarar an yada agarose, ba da izinin nunin faifai don sanyaya da ƙarfi a kan sanyi farfajiya ko yanki mai sanyaya. Wannan matakin dole ne a yi yadda ya kamata don hana agaroose daga saiti ba tare da izini ba, wanda zai iya shafar ganin abubuwan da ake gani a cikin bincike.Lysis na sel a kan nunin faifai
● abun ciki na bayani
Magani mafi sani na yau da kullun yana dauke da babban taro na salts da kayan wanka, kamar su tazara sel da kuma cire membranes yayin barin DNA m.Hyisi ● hanya da yanayi
Rarrada nunin faifai a cikin lyis bayani mafi sani da kuma a cikin 4 ° C na akalla 1 hour, ko da yake na dare ne sau da yawa fifiko. Tabbatar da tabbatar da tanki na lysis ya guji kawar da shayarwa da gurbatawa. Yanayin matakin lymes yana da mahimmanci don cire wuraren da aka cire salula ba tare da lalata DNA ba.● Muhimmancin cikakken ilimin tantanin halitta
Rashin kammala Lysis na iya haifar da fore-foret mara kyau da bayanan da ba wanda ba wanda ba za'a iya ba da amana. Sabili da haka, yana da mahimmanci don bin yanayin lysmerol da yawa, tabbatar da bayani yashi duk sassan zamewa da kuma lokutan shiryawa sun isa ga cikakken rushewar tantanin halitta.Alkaline mara son kai da kuma yanayin lantarki
● Shirya maganin alkaline
Maganin alkaline, sau da yawa cakuda na naku da EDTA, ana amfani dashi don kwance DNA. Yakamata a yi shirye-shiryenta a hankali, tabbatar da daidai ph da morarity. Freshly shirya mafita don kiyaye ingancinsa.● Alkaline manta mahimmanci da lokaci
Acubate nunin faifai a cikin alkaline bayani, yawanci na 20 - mintuna, don ba da damar DNA Strands don sakawa. Wannan matakin yana da mahimmanci don ganowa guda - Strand hutu da alkali - mahalun labile. Inganta lokacin da ba a kula da shi ba dangane da takamaiman tsarin yarjejeniya da nau'in tantanin halitta.Saurin saiti da sigogi
Kafa tanki na lantarki tare da zakarancin mai sanyi mai sanyi. Sanya nunin faifai a cikin tanki, tabbatar da cewa an nitse su sosai. Aiwatar da ƙarancin wutar lantarki (yawanci kusan 25 v) na 20 - minti. Yanayin iyakokin zabura, gami da wutar lantarki da lokaci, dole ne a fi dacewa don cimma mafi kyawun ƙudurin DNA Hijira mafi kyau.Rashin daidaitawa da kuma tsallake tsari
Hukumar samun shiri mai amfani da amfani
Bayan Elleroforeses, dakatar da nunin faifai don dakatar da dauki kuma daidaita DNA. Buffer na yau da kullun ya ƙunshi tushe na scis a pH na 7.5. A hankali kurkura cikin nunin faifai a cikin wannan Buffer sau da yawa don cire duk alamun kayan alkaline.● Fuskar fasahar
Tarkon nunin faifai tare da DNA mai kyalli DNA - Girmanci fenti, kamar enthidaum brother. Ethidium bromomide, yayin dazuzzuka, yana buƙatar kulawa da hankali saboda kaddarorinta na mutawic. Sybr kore yana ba da mafi aminci ga madadin tare da babban hankali. Sanya nunin faifai a cikin yanayin takin don lokacin da ya dace (yawanci 15 - minti) a cikin duhu.● Ganin hangen nesa na DNA Comets
Bayan an cika shi, kurkura swidides a hankali don cire wuce haddi tabo. Bincika nunin faifai a ƙarƙashin mai kyalli microscope. Ingancin taci da ƙudurin microscope suna da mahimmanci don samun hotunan share hotuna na kakar su.Microscopy da na hoto na hoto
CLOOSINALINGILIN NASARA
Wani mai kyalli microscope sanye take da saitin tace da ya dace wajibi ne don hangen nesa. Zaɓi microscope tare da babban ƙuduri da tsarin kyamara da zai iya ɗaukar cikakkun hotunan hotunan sarakuna.● Hotunan hoto - Hotunan inganci
Kama hotuna a kalla comets 50 a kowane slide don tabbatar da mahimmancin ƙididdiga. Tabbatar da haske da mai da hankali don guje wa kayan tarihi. Ingantaccen daidaituwa na microscope da saunan kamara na iya haɓaka ingancin hoto sosai.● Software don bincike na farko
Akwai shirye-shiryen software da yawa don bincike mai ban sha'awa, irin su calletcore da kuma Caske. Wadannan shirye-shirye suna auna sigogi kamar tsayin wutsiya, ƙarfin wutsiya, da lokacin wutsiya, waɗanda suke da mahimmanci ga lalacewar DNA.Shirya matsala na yau da kullun
Matsalolin gama gari
Batutuwa na yau da kullun a cikin amintattun masu walƙiya sun haɗa da babban sihiri, ƙarancin tsari, da rashin daidaituwa sakamako tsakanin nunin faifai. Waɗannan matsaloli na iya haɓaka daga kafar hanyoyi da yawa, gami da ƙarancin kulawa sel, saukarwa mai shirye, da kuma yanayin lyssis.● Santassi da matakan kariya
Don rage girman fitilun mai haske, tabbatar da cewa duk reagents da gels suna da 'yanci daga crassing. Don ƙarancin tsari, tabbatar da cewa tantanin halitta yana da girma, kuma yanayin lysis yana da kyau sosai. Cikakken Tsarin Slide shiri da cikakken sakamako masu ma'ana sune manyan matakan rigakafi.● tabbatar da martaba da daidaito
Samun haifuwa a cikin Amintaccen Assays yana buƙatar daidaitaccen matakin ladabi da ingancin kowane mataki na yau da kullun. Kayan aiki akai-akai a kai, yi amfani da babban - Refents Ingantaccen Realents, da kuma kula da cikakken bayanan dukkanin yanayin gwaji don tabbatar da daidaito.Fassara bayanai da sakamakon rahoton
● Nazarin sigogin wutsiya
Scartalan sigogi don lalacewa DNA wanda ya haɗa da tsayin wutsiya, wanda ke nuna girman girman DNA, da ƙarfin wutsiya, wanda ke nuna adadin DNA a cikin wutsiya. Lokacin wutsiyar zaitun, lokacin hadin kai, ana amfani da shi da yawa.Lissafin ilimin lissafi a cikin rahoton bayanai
Cikakken bincike na ilimin lissafi yana da mahimmanci don fassarar bayanai. Yi amfani da gwajin ƙididdiga mai mahimmanci don kwatanta matakan lalata na DNA tsakanin ƙungiyoyi daban-daban. Tabbatar da cewa bayanai daga mahara masu nunin faifai da gwaje-gwaje ana ɗaukar su don yin lissafi.● Gabatarwa da fassarar binciken
Gabatar da sakamakon ku a bayyane kuma a taƙaice hanya, ta amfani da zane-zane da tebur don misalta binciken maɓallan. Tattauna kan abubuwan lalata DNA an lura da kuma yiwuwar tasirin kan tsarin nazarin halittu a ƙarƙashin binciken.Kammalawa: cimma matsi mai kyau - inganci ASY STIDS
Ana shirya High - Ingantaccen ASSY STEDS ya ƙunshi hankali da zaɓi na zabin ƙirar, Aikin Shipple, yanayin lysis, yanayin lysis, mawuyacin yanayin. Kowane mataki dole ne a inganta shi da daidaitaccen tsari don tabbatar da sakamako ingantacce da sake haifuwa. Hadaukar hoto tare da mai da ake zargi da kayan adon mai ba da izini da masana'antu na iya inganta ingancin asirinku ta hanyar samar da ƙananan kayan aiki da tallafin fasaha.● Game daIphayanBiOSCIENS
Headquartered in North Wales, Pennsylvania, IPHASE Biosciences is a "specialized, novel, and innovative" high-tech enterprise integrating research, development, production, sales, and technical services of innovative biological reagents. Levorging mai mahimmanci da so na binciken kimiyya, ƙungiyar kimiyya ta fi ƙwarewar ƙwarewar ilimin kimiyya a duk kokarinsu na kimiyya don taimakawa cimma manufar binciken su. Bincika da R & D kyakkyawan na "registing na gaba", Iphayan sun kafa wurare da yawa na Amurka, Turai, da kuma kasashen waje na Asiya - suna rufe murabba'un Asiya 12,000.Lokaci: 2024 - 08 - 28:05:20.