Ka'ida
IPHASE'sPBMC keɓe kayans sun dogara ne a kan hanyar centrifugation na zamani mai yawa, wanda ya dogara ne akan bambancin daidaita saurin ƙwayoyin jini daban-daban a cikin matsakaicin gradient mai yawa don cimma rabuwar tantanin halitta. Haɗe a cikin kit ɗin akwai ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙaƙƙarfan rarrabuwar kawuna wanda ke haifar da tsayin daka mai ƙarfi yayin centrifugation. Lokacin da aka ƙara samfurin jini gabaɗayan anticoagulated sannu a hankali akan maganin rabuwa, sel masu yawa irin su jajayen ƙwayoyin jini da granulocytes sun zauna zuwa kasan bututun centrifuge, yayin da ƙananan ƙananan PBMCs an dakatar da su a tsaka-tsakin tsaka-tsakin bayani da plasma, suna samar da fili, fari, sararin samaniya na sel. Ta hanyar tattara wannan Layer na sel a hankali da wanke-wanke da centrifugation na gaba, ana iya samun babban tsafta PBMC.Takaitaccen gabatarwa
IPHASE's PBMC Isolation Kit kayan aiki ne da aka ƙera don masu bincike don keɓe PBMC da kyau da kyau daga duk jinin da ke da kariya. Kit ɗin yana da abubuwa masu zuwa:1.Highly m rabuwa: yin amfani da wani gyara yawa gradient SEPARATOR tabbatar da sauri da kuma m rabuwa na PBMC a lokacin centrifugation, yayin da rage samu daga sauran cell iri.
2.High tsarki: ta hanyar tsari mai kyau na rabuwa da matakan wankewa na gaba, za'a iya samun PBMC mai tsabta mai tsabta, samar da tushen abin dogara ga al'adun tantanin halitta na gaba, gwaje-gwajen ilimin kwayoyin halitta da sauransu.
3.Sterility da ƙananan endotoxin: maganin keɓewa da sauran abubuwan da ke cikin kit ɗin an yi su da tsauraran magani na aseptic da ƙananan matakan endotoxin, tabbatar da cewa ba za a gabatar da abubuwan da ba a so ba yayin tsarin keɓewa.
4.Widely m: ba kawai dace da mutum PBMC kadaici, amma kuma samar da musamman PBMC keɓe kaya ga sauran dabbobi nau'in (kamar biri, kare, bera, linzamin kwamfuta, da dai sauransu) don saduwa daban-daban bincike bukatun.
5.Easy don aiki: Kit ɗin yana sanye da cikakkun umarnin aiki da kayan aikin taimako masu mahimmanci, wanda ke sa tsarin keɓewa gabaɗaya mai sauƙi da sauƙi, har ma don farawa don farawa da sauri.
Misalai na aikace-aikace
An yi amfani da na'urorin keɓewa na PBMC na IPHASE a cikin bincike-binciken ƙwayoyin cuta daban-daban. Alal misali, a cikin bincike na rigakafi, masu bincike zasu iya amfani da PBMC da aka raba don rarrabawa da nazarin aikin kwayoyin T, kwayoyin B da sauran kwayoyin rigakafi; a cikin binciken oncology, ana iya amfani da PBMC don tantance matsayin rigakafi na marasa lafiya da ƙari da allon anti - ƙwayoyin rigakafi na ƙwayar cuta. Bugu da kari, ana amfani da kit din sosai wajen bunkasa alluran rigakafi, tantance magunguna da sauran fannoni.Kammalawa
Kit ɗin keɓewar PBMC na IPHASE ya ɗauki matsayi mai mahimmanci a fagen binciken ilimin halittu tare da kyakkyawan aiki da ƙimar aikace-aikacensa mai faɗi. Tare da ci gaba da ci gaban kimiyya da fasaha da karuwar buƙatar bincike, IPHASE za ta ci gaba da ba da kanta ga haɓaka ingantaccen, tsafta da sauƙiLokacin aikawa: 2024-07-27 16:30:36