A fagen bincike na al'ada da kwayoyin halitta, fahimtar bangarorin salula suna da mahimmanci don watsa rikice-rikicen aikin salula da hanyoyin cutar. Daga cikin wadannan bangarorin,tritosomes-A karami - sanannu amma mahimman aji na lysososome - organeles-da ke da alaƙa da ƙara hankali, musamman a cikin samfurin rodent.Bat tritosomesBayar da basira mai mahimmanci a cikin ayyukan enzymatic, rikice-rikice na rayuwa, da kuma shekaru masu alaƙa - suna da mahimman batun a cikin rassan ilimin halitta daban-daban.

Wannan labarin yana binciken abin da scitosomes ne, yadda aka ware su da kuma halin ci gaba a duk faɗin yankin kimiyya.

Fahimtar tritosomes: ƙwararrun ƙwararru

Tritosomes subtypes ne na lysosomes na lysosomes, wanda ke da membrane membrane - ɗaure ko cututtuka da ke cikin lalata macradation na macradecolecules. Yayinda daidaitaccen Lysosomes suna dauke da mahaɗan enzeyes da aka yuwu, tritosomes ana wadatar da su a cikin hydroles na musamman da ke bambanta su daga yawan mutanen da ke al'ada.

An fara gabatar da kalmar "tritosome" a cikin karatu da nufin kara ƙaddamar da lysosomes ta amfani da daban-daban da kuma m gerrifugation. Ta hanyar amfani da irin waɗannan dabaru, masana kimiyya sun sami damar gano subpopular-kamar tritosomes - wanda ya banbanta a cikin enzyme abun ciki, bensity, da aiki.

A cikin berayen, tritosomes an yi nazari sosai saboda kasancewar sashin; takamaiman alamomi na enzyme da babban repitence iyawar ƙwayoyin cuta a cikin kyallen takarda.

Ware da halayyar bera na bera

Kerewa na bera tritosomes yawanci ya shafi cin nasara ko kuma percoll gradient na percoll gradientation mai biyo bayan nama homogenization. Ana amfani da kyafaffen hanta, kamar yadda hepatocytes suna da mahimman subpapulas da Lysosomals masu yawa.

Matakai na maɓalli a cikin trapoome daga cikin trapoome ya haɗa da:

• Homogenizing ber hanta ko koda koda a karkashin yanayin sanyi don adana amincin enzymatic.
• Daban-daban centrifugation don cire nuclei da mitochondria.
• Yankunan grentity gradient zuwa ware tritosomes daga al'ada lysosomes da sauran vesicles.
• Enzyme Assayis don gano tritosome - takamaiman ayyuka, sau da yawa amfani da alamomi kamar β - glucuronidase, α - mannosidase, α - mannisidase, ko arylulfatase.

Da zarar an ware shi, tritisomes na Tarihi na Tarihi, da kuma kusancin ƙwarewar karatu don yin nazarin tsarinsu, tsarin abubuwa, da kuma halaye masu aiki.

Me yasa ake amfani da samfurin bera don nazarin tritosomes?

Berayen ƙirar ƙirar samfurin ne don karatun salula da rayuwa saboda kamanninsu na zahiri ga mutane, kwanciyar hankali, kuma da kyau - ya kafa matakan tattara ɗabi'a. A bera hanta, musamman, wata hanya ce ta enzosomal enzymes kuma yana zama mai ingantaccen samfurin don nazarin Endo - Lysosomalysomal.

Bat tritosomes suna da amfani musamman a cikin bincike mai gamsarwa a:

• Cututtukan ajiya na lysosomal
• Tsufa da Bearesence
• Nama - takamaiman metabolism
• Magungunan ƙwayoyin cuta da guba

Daidaitawa a cikin isolating tritosomes daga bera nama ya sanya su tsarin ma'amala ne don nazarin heterogeneity, wanda aka ƙara sanin shi azaman mahimmancin cututtuka da yawa.

Aikace-aikacen Bat tritosome na karatu a cikin binciken biomededical

1

Tritosomes ana wadatar da takamaiman enzymes wanda ke da rauni ko rashin ƙarfi a cikin LSDs. Nazarin waɗannan kwayoyin halitta suna taimakawa wajen tantance yadda yanayin enzyme ke haifar da substrate kuɗi da kuma ɗumbin salula.

Misali, masu bincike sun yi amfani da harkar bashin da za su fahimci hanyoyin enzymatmat ba a cikin cutar enzeuties.

2

Shekaru - canje-canje masu alaƙa a aikin Lysosomal sune zauren salon salon salon. Karatun Tritosome a Berayen Berayen sun gabatar da tarin LIPOFusCINC, da kuma yin saurin fasalulluka na sel na sassauƙa.

Wannan yana sanya bera tritosomes kayan aiki mai mahimmanci don bincike Shekaru - Associatedwar hanyoyin magance matakai, gami da wadanda aka haɗa zuwa neuringeration da kuma bata lokaci.

3. Toxicology da Pharpacokinetics

Lysosomes, gami da tritosomes, suna da hannu a cikin sequestration da lalata na Xenobiotics. Ta hanyar yin nazarin bera Ana sarrafa magunguna da gubobi na muhalli a matakin salula.

Bayanan martaba na Enzyme a cikin Tritosomes kuma iya taimakawa wajen hangowar kwayar - takaddar da kwararru miyagun ƙwayoyi, tana tallafawa ci gaban mahimman magunguna.

4. Bishiyar tantanin halitta

Bat tritosomes ya ba da damar masu bincike wajen kwatanta halayen lysosomal a kan jinsuna da kyallen takarda. Wannan yana da amfani musamman lokacin da nazarin jinsi - takamaiman kabin enzyme, tsarin ɗabi'a, da kuma daidaitawa na metomolic.

Nazarin dabarun bincike da aka yi amfani da su a cikin binciken tritosome

Nazarin bera na bera yana amfani da hadewar hanyoyin gargajiya da zamani:

• Enzyme aiki Ayyuka don ƙarin bincike na hydrolaes.
• Buguwar yamma da taro na farko don gano furotin.
• Immunoflusscence da rikodin micracopy don karatun gida.
• Lipidomics don tantance membrane abun ciki da tsarin ajiya.

Waɗannan kayan aikin suna bawa masu bincike damar tsara ayyukan aikin tritosomes a cikin salula na kwastomomi da ilimin cututtukan jini tare da babban canji.

Iyakoki da ra'ayoyi na gaba

Yayinda bincike na banki tritosome ya ba da ma'anar fahimta, rashin iyaka da yawa ya kasance:

• Heterogeity na lysosomes na iya wahalar da fassarar sakamakon subracationation.
• Nau'in - takamaiman bambance-bambancen dole ne a yi la'akari lokacin da sauƙaƙe bayanai zuwa ilimin ɗan adam.
• Ana buƙatar daidaitaccen ladabi don inganta haifuwa a duk labs.

Duk da waɗannan kalubalen, sha'awar a cikin substypes kamar tritosomes yana haɓaka, musamman a cikin mahallin satar salula, da siginar sasantawa. Bincike mai zuwa na gaba na iya mai da hankali kan haɓaka masu kyalli ko rayuwa - dabarun tantanin tantanin halitta don yin nazarin hanyoyin da ke da ƙarfi a cikin ainihin lokacin.

Ƙarshe

Bat tritosomes wakiltar tsarin salula na musamman da ba da labari wanda ya taka muhimmiyar rawa a ilimin halittar Lysososomal. Ta hanyar ware da kuma rarrabe wadannan kwayoyin, masu bincike sun sami damar zurfafa fahimtarsu game da ka'idar rayuwa, shekaru - Likitawa masu dangantaka, da hanyoyin cuta.

Yayin da al'umman kimiyya ke ci gaba da bincika abubuwan da ke cikin ƙasa, bera bera tritosomes zai kasance mai mahimmanci mai mahimmanci don watsa abubuwan da hanyoyin cututtukan cututtukan cututtukan fata da cuta.