Ulo Nnyocha CGT Mphase òké (c57bl / 6) PBMC, kpọnwụrụ akpọnwụ

Mphase òké (c57bl / 6) PBMC, kpọnwụrụ akpọnwụ

Nkọwa mkpụmkpụ:

A na-ekewa ngwaahịa a site n'ọbara C57bl / 6 site na njupụta nke afọ ojuju Convent Centurent centunt Centrifgation na ndị otu sel na-ahụ maka otu mkpụrụ ndụ) na monocytes nwere otu nucleus. A na-ejikarị ya na nchọpụta ọgwụ / mmepe, nyocha na mmebi nke ndị ọzọ - Ihe ọmụmụ metụtara.

Kpọtụrụ ugbu a Tinye na Cartzụ ahịa

Nkọwa nke ngwaahịa

Mkpado Ngwaahịa

Udi:
Pọọsụ ọbara mononiclearl, PBMC
Ihe mba.:
082e21.21
Ogo nkeji:
5mi
Umu:
C57bl / 6
Ọnọdụ Cell:
kefriza
Ọnọdụ nchekwa na ụgbọ njem:
Akọrọ ice / mmiri mmiri nitrogen
Isi iyi nke anụ ahụ:
C57bl / 6Musipierilalblod
Akwukwo nyocha:
Na vitrolis metabolism nke ọgwụ

Nke gara aga: Iphase òké (Balb / c) PBMC, kpọnwụrụ akpọnwụ

Osote: Iphase òké (ICR / CD - 1) PBMC, kpọnwụrụ akpọnwụ