Lysimal kwụsiri ike: Ihe dị mkpa na arụmọrụ ADC na Sirna maka nkwalite ọgwụgwọ ọgwụgwọ

Keywords: Nchịkọta Njikọ, Imeju Lysity, Lyssimal Catabilism, Lys8201A, GGFG - DXD, Galnac- -SERNA, Sirna Mwepụ, Sirna Spen, Hepatocyte Lousimes, tritosome, lysimomal acid phosphatal phosphatal

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Ndubata

Ọganihu na nke Na-ezipụta evolushọd na - ebupu ozizi ma antibody - ogwu conjugates (adcs) na RNA, dị ka ọgwụ uma. N'agbanyeghị ebumnuche ha dị iche iche na usoro ha, ma Adc na Sirca na-erute ntụkwasị obi naImeju Lorsosomegburugburu, ebeLyssomal kwụsiri ikenaLyssosome CatabolismGwuo ọrụ pivotal. Na usoro ADC, ịpụ na nke aNjikọ Adminer by CatEpsin B-Ndi na DS8201A naGGFG - DXDNyiwe - Angures na-achịkwaNhazi Ebube. Maka Sarna ọgwụgwọ, meriri mgbochi Lyssomal dị mkpa maka ịrụ ọrụNtinye SirnanaSirna gbapụrụ, karịsịa jiGalenac-Sirnaconjugates na ebumnuchehepatocyte lssisomes. Akwụkwọ a jikọtara ọnụ na-enyocha ụzọ ndị a na nsogbu ndị a.

1. Adcisview na echiche isi

ADC bụ ọgwụ ọgwụ na-eme ka ihe mgbochi monocnal, onye na-akwụ ụgwọ cytotoxic, yana Njikọ Adc. Ezubere njikọ ADC a iji hụ na ịtọhapụ ego, na-ezube ị na-eleba anya na mgbe a na-echebe anụ ahụ dị mma. Lookmanye ego a na-achịkwa na-achịkwa na-adabere na imeju na-emeju, ebe mgbakwụsi ike na-eme ka ọ bụrụ nnukwu catabolism. N'ime ntọala a, CatEpsin B ga-arụ ọrụ n'oge kwesịrị ekwesị iji gbazie adc Njikọ Njikọ. Iji maa atụ, DS8201A na-ahụ maka GGFG - DXD usoro iji mezuo ụgwọ ezughị ezu na imeju na imeju, na-ejide ma ọ dị mma ịme ihe na-egbu egbu.

Usoro Njikọ ADC na Ntinye Aka

Nhazi nke Adc Njikọ dị oke mkpa maka ịchekwa ụgwọ ịkwụ ụgwọ na-ejide ya. A na-emetụta nkwụsi ike na ọnọdụ n'ime imeju na-emeju, ebe nkwụsi ike lysival na-arụ ọrụ dị mkpa. Onye na-akwụsi ike Lyposome na-eme ka Catabolism dị irè, na-ejide na enzymes dị ka CatEpsin B nwere ike ịrụ ọrụ nke ọma na adc. Na onodu nke ndọghachi ego, onye na-ahụ maka ihe Adc ga-enwerịrị ike na mgbasa ma ọ bụrụ na a ga-agbali ya na imeju LOSOSOME. Nke a na CHITSIN B, nke dị mkpa maka ịkpali Lyssoomes Catabolism. Ọzọkwa, usoro dị elu dị ka DS8201A na GGFG - DXD na-emeju na imeju na-emeju na gburugburu, na-eme ka ọrụ Njikọ ADC na-akwụsi ike na-akwụsi ike na-akwụsi ike.

Na mgbakwunye na CASEPSIN B, ụlọikwuu Cysteine ​​ndị ọzọ dịka Catepsin L, CatEpsin m, na CatEpsin K na-atụnye ụtụ na nhazi ọgwụgwọ ọgwụ na ịgha mkpụrụ ọgwụ ọjọọ. Cattopsin l a na-amata nke ọma maka ọrụ EndopyTidase ya na ọrụ ya na imebi protein protein, si otú ahụ na-akwado ntọnye ụtụ dị irè. N'otu aka ahụ, Catiopsinm, n'agbanyeghị na ọ dị obere amata, na-ekerela na catabolism Lyssimal ma nwee ike na-eme ka ọrụ nke ndị ọzọ na-eme ihe. Cattopsin K, onye ama ama maka ọrụ nchịkwa ya na Resertionlọ ọrụ ọ bụla, nwekwara ike ịdọ aka na paptip njikọ n'okpuru ụfọdụ ọnọdụ. A na-eme ka ihe ndị ọzọ na-akwụ ụgwọ na mgbe ụfọdụ na-akwụ ụgwọ na usoro ndị a na-akwụ ụgwọ na usoro nchịkọta Adc na ndenye ego eji arụ ọrụ na-arụ ọrụ na-eme ka ọ rụọ ọrụ ọgwụgwọ na-akwụ ụgwọ na mgbasa setịpụrụ. Inyocha nyocha ọzọ n'ime interplay n'etiti katipsin b, cadiepsin l, CatEpsinm K.

2. Sirnna ọgwụgwọ na ihe ịma aka

Sirna nnyefe na ntinye Lysimal

Ọgwụ Sirna na-enye nkọwa dị elu site na usoro otiti; Agbanyeghị, nsogbu dị mkpa na-adọrọ mmasị na sirna gbadara mbibi. Mgbe endocytosis, obere pere mpe nke Spatosomes na imeju na hepatocyte lassosomes, ebe horsosolilis catabolism - nke a na-eme site naLysisomal acid phosphatase-Chilite kwụsiri ike ma na-eduga na ngọda.

Usoro nke Galnac- -Sirna Conjugates

Galenac-Sirna Perjigates Kwalite Sirna site na ichebe Asigoglycotein na Hepatocytes, nke na-akwalite mkpọchi ngwa ngwa. Ozugbo n'ime, conjugatis ga-emeri ihe mgbochi Lyssoomal iji mee ka ndị na-agbapụ sirna dị mma. Nkọwapụta kemịkalụ dị ka 2'-f, otu f, na phosphorothiote chekwara sirna ma hụ na sistemụ nnyefe Sija na-efe efe na gburugburu imeju na-emeju.

Usoro nyocha metabolic na nhọrọ nke Oligonucleotides

Dị ka ọ dị na obere molecule ọgwụ ọjọọ, usoro ihe atụ chọrọ n'ezie na Virro metabolic ọmụmụ ihe n'oge mmepe. Ọmụmụ ihe ndị a na-enyocha mmetụta nke Lyssomolism Catabolism na ọrụ nke lySosomal acid phospharase na-emebi syna n'ime imeju na-emeju na wpatocyte. A na-ekwusi ike na-eme ka nnyefe Sirna ma na-ejide Orna siri ike zuru oke. Usoro ule dị iche iche - dị ka imeju honogenates, linsomomes na-emeju, na isi ọwụwa isi na-arụ ọrụ - na-arụ ọrụ iji memic gburugburu ebe obibi. Idozi nkwụsi ike nke Lyssimal site na Ntụle ndị a bụ isi ihe iji mee ka arụmọrụ Salna na-eme ka arụmọrụ nke sirna.

3. Ọrụ nke imeju lysisomes

Mejun Live Lysososome

Ma ADC na Sirda Conticies na-eme ka imeju na imeju - ihe dị mkpa maka ịme mgbanwe na mmebi iwu. Na sistemu ADC, imeju Likemome na-eme ka onye na-edebe ego na-achịkwa site na Katissianssin byc. Na usoro ọgwụgwọ, imeju lposoome (na hpatocyte lssiomomes) na-enye mgbochi n'ihi ihe ike Lysi catabolism na ọrụ nke lySosomal acid phosphatase. N'ihi ya, ijigide nkwụsi ike Lysity dị elu bụ isi ihe iji gbochie Catabolism nke ọma maka ma na-achịkwa Syna.

Na virusto models na usoro nyocha metabolic

Iji mụọ ma ahapụ ma akwụghị ụgwọ nke Salna na Sarna na-eji ọtụtụ n'ime vitrodị vitro.TritosomeModdị - dị ka oke ime obodo tritosomes-na-enye usoro amụma iji nyochaa catabolism na Lyssomal kwụsie ike. Na mgbakwunye, usoro nyocha metabolic tinyere afọ irighiri akwara, imeju na-emeju, na mbụ Hepatocytes na-enyere aka ịtụle ụgwọ ọ na-arụ na-eme ka ịkwụ ụgwọ ya na ngọngọ nke ọma. Modelsdị ndị a na-egosi mkpa ọ dị mkpa iji dozie catabolism Lyssomal Phosphatal phosphatase iji nọgide na-arụ ọrụ imeju Line Lysing.

4. Ntinye ọnụ maka usoro maka nsonaazụ ọgwụgwọ ọgwụgwọ

Ihe ịga nke ọma nke usoro ọgwụgwọ na nke Sirna na-adabere na weghaara nkwụsi ike ma na-achịkwa Catabolism Lyssomal. Maka adcs, na-emezi ihe ejikọtara Adc njikọ ma na-akwado ka Cathapsin BIRICS BIRICE (dị ka gosipụtara naDS8201Ana GGFG - DXD Sistemu) dị oke mkpa. Maka usoro ọgwụgwọ Sirna, mmiri mmiri nke Galinac-Syna comjogates na usoro iji belata ọrụ nke phospharal acid phosphaala na Sirna. Akụkụ nke a na-eme na-ewere ọnọdụ pụrụ iche nke imeju na imeju bụ ihe dị mkpa iji nweta nrụpụta ọgwụgwọ dị elu.

Mmechi

Ma ADC na Sarna na-eche nsogbu ndị a na-ahụkarị na imeju, ebe nkwụsi ike Lyssomal na Lyssoome Catabolism na-ekpebi ihe ịga nke ọma. Sistemụ ADC, ọkachasị DS8201A na GGFG - DXD, dabere na onye Catiepsin b maka ntọhapụ ụtụ isi. N'otu aka ahụ, nnyefe nnyefe nke Salnac-silna na-aga agha agha ga-egbu egbu willonomal ma mebie site na Lysisomal Acid iji nweta mgbapụ dị mma nke ọma. Site na ngbanwe na Vorro Models dịka tritosomomes na imeju S9 nke irighiri ihe na-eme ka ọ gbanwee ma ọ bụrụ na Adcom na Syna na-enweghị atụ.