Iphase bwino kwambiri C57BL / 6 mbewa yoyamba hepatocyte

C57BL / 6, nthawi zambiri amatchedwa "C57 Black 6", "C57", kapena "zakuda 6"), ndi vuto lodziwika bwino la mbewa ya laborato. Imagwiritsidwa ntchito ngati mbewa yopanga ma genetic kuti muchepetse zofooka za anthu. Ndi imodzi mwazomwe zimagwiritsidwa ntchito kwambiri komanso zogulitsa zogulitsa mbewa chifukwa chotha kugwiritsidwa ntchito ngati vuto lodabwitsika, limalephera kusinthika, komanso kusinthika kwake.

Ⅰ. Chiyambi ndi mawonekedwe a C57BL / 6 mbewa

Mu 1921, C.C. Kutalika Kwa Abble Abby Lanthro kupssinjika kutulutsa zingwe zingapo zogudubuza nyama; Akazi a No. 57 omwe ali ndi wachimuna wa No. 52 kuti apeze C57. Anakonza ubweya wa ubweya wa C57 ku Brown ndikutchedwa C57BR (Brown), ndipo pokonza ubweya wakuda, wotchedwa C57BL (Black). Monga pali mbewa zambiri za mbewa ya C57, C.C. Mzere wina wopita pakati pa 1937, analemba C57BL / 6.

Kuphatikizidwa ngati "muyezo" womwe wabzala, mbewa ya C57BL / 6 imapereka chithunzi cha majini ambiri osunthika ndipo ali ndi mikhalidwe yathupi.

Kuphatikiza apo, C57BL / 6 ndi mtundu wa nyama yoyesera ndi zolondola, zofananira, komanso zopsinjika zopsinjika. Zotsatira zake, C57BL / 6 idakhala ndi mbewa yoyamba kuti ikhale ndi mtundu wake, ndipo nthawi zambiri amagwiritsidwa ntchito pofufuza za mtima, zachuma, chilengedwe, chilengedwe, komanso neurobilogy, ndi neurogogy; Zakhala zikugwiritsidwanso ntchito pophunzira zizolowezi, zovuta za neuroode (e.g., matenda a metabolic, kutayika kwa matenda a matenda ashuga, kodi kulolerana ndi matenda am'mimba.

Ⅱ. Kuyerekeza kwa C57BL / 6 mbewa

Mu 1947, labotor labotale idayambitsidwa C57BL / 6 kuchokera kochepa ndikuzitcha C57BL / 6j. Mu 1951, dziko la National Institute of Health (NIH) adayambitsa C57BL / 6J2nd m'badwo wa Cirline ndi Wopangidwa / 6n kuchokera ku labota ya Jackson. Monga pali zosiyana zambiri pakati pa zigawo, ndikofunikira kusankha mbewa yoyenera molingana ndi zoyeserera.

Kuphatikiza apo, C57BL / 6J ndi C57BL / 6n adasankhidwa monga zoyesera zinyama, pamakhala zosiyana pang'ono pakati pawo.

Ⅲ. Kufunika kwa C57BL / 6 Mouse yoyamba hepatocytes

Maselo oyamba a hepatocytes ndi maselo a chiwindi a chiwindi amatalikirana mwachindunji kuchokera ku ziwindi za nyama, zomwe zili ndi zabwino zosasinthika.

A Hepatocytes wamkulu ndi ogwirizana ndi - Vivo malo, ndi miyambo ya ma enzyme ndi matope amafanana ndi kafukufuku wa thupi pafupi ndi - Statem Statem, Vivabolic Clives. Nthawi yomweyo, kugwiritsa ntchito kafukufuku woyamba wa hepatocytes a mu - Kafukufuku wa Vityro samayambitsa mayesero a nyama, ndikuchepetsa mtengo wa kuyesa kwa nyama. Monga pano, hepatocytes woyamba wa hepatocytes amagwiritsidwa ntchito kwambiri pazinthu zam'madzi komanso kafukufuku woyambira, monga protekecs, zodzikongoletsera ndi zigawo; Nthawi yomweyo, amathanso kugwiritsidwa ntchito m'makampani amakono odziwika bwino, monga kuwunika mankhwala, kagayidwe ka mankhwala osokoneza bongo komanso kafukufuku wa mankhwala a khansa, ndi zina zoyambirira.

C57BL / 6 mbewa, monga mawonekedwe wamba a nyama, okhala ndi zapadera koma zothandiza thupi. Chifukwa chake, kafukufukuyu ndi chitukuko chawo woyamba hepatocytes ndi wofunikira kwambiri pochiza matenda am'mudzi, matenda a mumitundu yambiri ndi mapangidwe a mankhwala!

Ⅳ. Iphase - Zojambula Zogwirizana

Kuyankha kuchitidwewu, Iphase, monga mtsogoleri wofufuza zachilengedwe ku - Kafukufuku wa CRRORL, agwira bwino ntchito za C57bl

Maukadaulo a Iphase bwino amasungunulidwa bwino hepatocyte yoyambirira ya hepacecyte kuchokera ku C57BL / 6 mbewa ndikuyesa ntchito ya kuyimitsidwa ndi mankhwala osokoneza bongo. Maukadaulo amagwiritsa ntchito ndende yomaliza ya 1 μm Verapamil ngati gawo lokhazikika, adatsimikiza kuchuluka kwa mankhwalawa pa 0 min, 5 min, ndikupeza kuti kuchuluka kwa mankhwalawa pang'onopang'ono kunachepa ndi ulemu nthawi. Anazindikira kuti theka - Moyo wa Vrapamil anali pa 28.51 min, ndi Clean Clearance Slatics inali yoyikitsitsa ya ma hepatocytes a C57BL / 6!

Kukhutisidwa

Zinthu za Iphase zimapezeka kuchokera ku magwero ovomerezeka ndipo zimadziwika bwino.

Umboni

Nyama zimayesedwa kwa othandizira opatsirana kuti awonetsetse chitetezo cha malonda.

Ntchito yayikulu

Maukadaulo a Iphase atsimikizira ntchito ya cyp450 cypyme pogwiritsa ntchito verapamil ngati gawo lapansi, ndipo zotsatira zopangidwa zimawonetsa kuti kukwaniritsa maphunziro a kasitomala.

Muyeso wobwezeretsa kwambiri

Kubwezeretsa kwa Crovophorsecy kumatha kupitirira 90%.

Zotheka

Iphase amatha kupereka ntchito zopangidwa ndi mitundu yamitundu yapadera komanso minofu malinga ndi zomwe makasitomala amafunikira.

Kuphatikiza pa kukhazikika kwaposachedwa kwa C57BL / 6 mbewa Hucytes Iphase amatha kupereka makasitomala osiyanasiyana ndi mitundu yosiyanasiyana ya gepatocytes pulayimale yothandizira makasitomala ndi mayesero osokoneza bongo.