Zotsatira zoyezetsa zosagwira? Mtundu watsopano wa in-vitro micronucleus test kit wafika kuti uthandize!

GAWO 01. Mu-Vitro Micronucleus Test

Ma micronuclei ndi ma chromatids athunthu kapena zidutswa za acentric kapena ma chromosome a mphete omwe amakhala mu cytoplasm pomwe ma chromosome amalowa m'maselo aakazi kupanga ma nuclei pambuyo pa mitosis. Chifukwa tiziduswa kapena ma chromosomes sangalowe mu phata lalikulu pa telophase, pamene maselo aakazi alowa mu interphase yotsatira, amalumikizana kukhala tinthu tating'ono kunja kwa phata lalikulu ndi kupanga ma micronuclei. Ndi kuwunikira kwa mapangidwe apangidwe, kufunikira kwa ma micronuclei, kuwongolera kosalekeza kwa njira yodziwira komanso kukwanira kwa njira zoyesera, kuyesa kwa micronucleus kunakhala imodzi mwa njira zazikulu zowonera masinthidwe. Amagwiritsidwa ntchito kwambiri kulosera za ngozi ya carcinogenic, kuzindikira ma inducers aneuploidy ndi zoopsa zina zama genetic.

The in-vitro micronucleus test ndi njira yoyesera ya genotoxicity yodziwira kupangidwa kwa ma micronuclei m'maselo a mammalian atachiritsidwa ndi chinthu choyezera. Ndiwoyenera kuyang'ana kusweka kwa chromosome ndi aneuploidy yopangitsidwa m'maselo a mitotic panthawi / pambuyo pokumana ndi mayeso. Poyerekeza ndi in-vivo test, in-vitro micronucleus test ndi yosavuta, yachangu, komanso yotchipa. Kuphatikiza apo, sizimakhudzidwa ndi zinthu monga kusiyana kwapayekha pakati pa nyama zoyesedwa. Popeza in- vitro micronucleus test imatha kuzindikira momwe ma micronucleus amachitira ndi mankhwala ena pamlingo wochepa wokhala ndi chidwi chachikulu. Choncho, wakhala akugwiritsidwa ntchito kwambiri m'munda wa genetic toxicology.


GAWO 02. In vitro micronucleus test kit

The IPHASE in-vitro micronucleus test kit imagwiritsa ntchito ma cell a Chinese Hamster Lung (CHL) ngati mayeso. Maselo a CHL amawonekera kuzinthu zoyesera ndi / popanda metabolic activation system ndipo amathandizidwa ndi actin polymerization inhibitor cytochalasin B. Maselo amaloledwa kufika pafupipafupi ma cell a binucleated, kenako amakololedwa, kukonzekera, ndi kuipitsidwa; ndiye kuchuluka kwa ma micronucleus a maselo omwe anamaliza mitosis imodzi (ma cell a binucleated) amawunikidwa pansi pa maikulosikopu kuti awone kuthekera kwa mutagenic kwa chinthu choyesera. IPHASE kit imapereka ma reagents onse ofunikira ndi ma cell poyesa in-vitro micronucleus. Popeza zida zonse zadutsa mayeso okhwima, zotsatira zoyeserera ndizolondola, zodalirika komanso zobwereketsa.

--Ubwino Wazinthu --

1. Zosavuta

Imasunga nthawi yopangira S9 ndikukonzekera reagent,. Zida zitha kugwiritsidwa ntchito mwachindunji, kufupikitsa kwambiri kuzungulira kwa kuyesa.

2. Kulondola

Chigawo chilichonse cha zida za reagent chayesedwa bwino kwambiri, kotero zotsatira zoyeserera pogwiritsa ntchito zida ndizolondola, zodalirika komanso zobwerezeka kwambiri.

3. Kukhazikika

Chidacho ndi chokhazikika komanso chosavuta kunyamula ndikusunga.

4. Kusinthasintha

Ntchito zosiyanasiyana, zitha kugwiritsidwa ntchito poyesa genetic toxicology yazakudya, mankhwala, mankhwala ophera tizilombo, mankhwala opha tizilombo, zowonjezera chakudya, zodzoladzola, ndi zina zambiri.

Kufananiza zida zatsopano ndi zakale

IPHASE imatsatira miyezo ya dziko lonse ndi malangizo a in-vitro micronucleus mayeso m'magawo osiyanasiyana monga chitsogozo chake chaukadaulo. Kuphatikiza apo, IPHASE imatenga mayankho amakasitomala ngati cholinga chake chokhathamiritsa kuti isinthe kapangidwe kake ndi kachitidwe kazinthuzo ndipo yakweza bwino zida zoyeserera za in-vitro micronucleus kuti zikwaniritse zosowa zambiri zamakasitomala ngakhale zitatsimikizira mosalekeza.

1. Mapangidwe Azinthu

Ma reagents opaka utoto a Giemsa ndi ma reagents ofunikira pokonzekera aphatikizidwa kuti zikhale zosavuta komanso zogwira mtima.

2. Njira

Protocol ya kit ndi mwatsatanetsatane, ndikuphatikiza zoyeserera zoyeserera ndi zina zomwe zili mkati kuti mupewe zolakwika wamba.

GAWO 03. Zogulitsa Zogwirizana