Mankhwala a Iphase a Sirna mankhwala mu kafukufuku
Mankhwala a nucleic acid, chifukwa chaukadaulo wawo wapadera, ndipo zayamba kuchitika mwatsopano m'zaka zaposachedwa. Mankhwala a nuclec acid amaphatikiza RNA yaying'ono (Sirna), antifting origanhuothides (Aso), MicRan RNA (MRNA), ndi mitundu ya gene. Mwa zina, Sina, monga hotspot, monga hotspot m'thupi la ma elecleic acid kufufuza kwa mankhwala, agwiritsidwa ntchito kwambiri mu kusintha kwa mankhwala, zotsatira zoyipa, zoyipa zoyipa, komanso kaphatikizidwe kosavuta. Akuyembekezeredwa kukhala mankhwala odalirika kwambiri owuma kwambiri opanga mankhwala pambuyo pa molekyulu yaying'ono ndi mankhwala antiod.
- 1. Kuzindikira kwamakina ku Sirna mankhwala
Kulowerera pang'ono kwa RNA (Sirna), kumatanthauzanso kuloza RNNA, RNA yaying'ono, kapena ma molekyulu ofupikirapo - nthawi zambiri. Pa synthesis, Sirna alowa cell kudzera pa endocytosis. Gawo laling'ono la Sirina kuthawa chiwonongeko cha lysosomal ndipo amalowa cytoplasm, komwe limaphatikizidwa mu RNA - Kukhazikika kuloza zovuta (RIC). Mkati mwa ric, Sisna usaumirire m'magulu awiri: Chikhulupiriro ndi chingwe cha anti. Chingwe chomwe chimawoneka chikuwonongeka msanga mu cytoplasm, pomwe ma ris, omangidwa ku chingwe cha antistinte, chimayambitsidwa. Zovuta nthawi yomweyo zimamangiriza mrna, kuwongolera cleavage ndi kuwonongeka kotsatira. Chifukwa cha kuwonongeka kwa a MRNA, mawonekedwe a gene omwe akufuna kuchepetsedwa, pamapeto pake amatsogolera ku Gene Stonetion ndi Choletsa cha Protein Translation.
- 2.
Ku Vivo, Barna Mankhwala amakambitsidwa makamaka ndi ma nyukiliya komanso kutumphukira m'magazi a plasma ndi minyewa, m'malo motalika ma metabolic mu chiwindi. Kutsatira zosintha, kupangira Sirna mankhwala owonetsera kuchepetsedwa kagayidwe m'magazi. Nthawi zambiri, mankhwala ambiri a Sirna amatengedwa ndi chiwindi, ndi gawo laling'ono lomwe limagawidwa kwa minofu ina, komwe kumapangidwa ndi manyuchi mu chiwindi kapena minofu ina. Chifukwa mu maphunziro a vivo ophunzirira matenda a Sivobolism, zinthu za metabolic zimadziwika ndipo zimasanthula kwambiri mu plasma, mkodzo, ndowe, komanso chiwindi kapena impso) kuchokera ku zinyama.
Komabe, mkati mwa mapangidwe osokoneza bongo, kuchuluka kwa nthawi yoyeserera, komanso ndalama zambiri zogwirizanitsidwa ndi maphunziro a Vivo omwe amakumana ndi mavuto akulu - Kupanga kwa mapangidwe apadera. Zotsatira zake, mu maphunziro a vits metabolism ndizofunika kwambiri panthawi yoyambirira ya Sirna mankhwala. Izi zimapereka Ubwino Wosadabwitsa, monga kuchuluka kwapamwamba komanso zazifupi zoyeserera, zomwe zimatha kupititsa patsogolo momwe Sirna wazakudya zojambula.
Gome 1: Mu vitro metabolism kafukufuku wa SIRANA mankhwala
| Gela | Karata yanchito |
| Seramu / plasma |
Yerekezerani kukhazikika kwa Sirina kwa Sirna mankhwala m'magazi ndi dongosolo lonse. Izi nthawi zambiri zimayesedwa kwa mu vita yamankhwala osokoneza bongo. |
| Chiwindi s9 | Ili ndi ma enzyme ambiri omwe amapezeka mu minofu ya chiwindi ndipo amapezeka mosavuta. Kufikira pamlingo wina, zitha kugwiritsidwa ntchito ngati choloweza mmalo cha chiwindi cha homogeni. |
| Chiwindi cha chiwindi homogenate | Makina a enzyme ndi okwanira kwambiri ndipo amalimbikitsidwa pakuwunika kwa Vitro ndi kuwunika kwa Sirna mankhwala. |
| Hepatocytes | Enzyme dongosolo ndi yokwanira, ndikupanga kukhala koyenera kuwunika kwa chiwindi - Sirna mankhwala. |
| Lysosomes | Lysosomes ndiye malo oyambira pomwe Sirna Mankhwala omwe Sirna amakumana atalowetsa maselo kudzera pa Endocytosis. Amakhala ndi enzyme ya enzyme, kuphatikiza nucleases ndi ma hydroses osiyanasiyana, ndipo ndi malo ofunikira a Sirna metabolism. Lysosomes amapereka njira yoyesera yoyeserera yophunzira kagayidwe ka mankhwala Sirna. |
- 3. Iphase Zoyenera
Mabungwe omwe amakhudzidwa ndi gwero lawo zopanga zawo kudzera munjira zovomerezeka, zomwe zidachokera.
Chitetezo
Onse ogwira ntchito komanso nyama zonse zopangidwa ndi matenda omwe amathandizidwa kuti awonetsetse kuti ndi otetezeka.
Kuyera Kwambiri
Chiyero cha foni chimatha kupitilira 90%.
Kukula Kwambiri
Kusintha kwa maselo kumatha kupitilira 85%, kukumana ndi zosowa za kasitomala.
Muyeso wobwezeretsa kwambiri
Mlingo wobwezeretsa utatha kupitirira 90%.
Kusinthasintha
Ntchito zamachitidwe amapezeka potengera zofunikira za makasitomala, kupereka mitundu yapadera kapena minofu ya cell cell.
| Magulu | Zinthu za Iphase |
| Magawo azofunikira | Chiwindi lysosomes |
|
|
|
|
|
|
|
|
| Akuluakulu a hepatocytes | Kuyimitsidwa hepatocytes |
| Zovuta za hepatocytes | |
| Plasma | Plasma kukhazikika |
| Plasma protein kumanga |
Post Nthawi: 2024 - 12 - 20 13:08:46