Spleocytes mu kafukufukuyu: mitundu ndi kudzipatula, kuzizira, zopumira

Mawu osakira:Spleocyte (spls); Monnomolgus spanocytes; Anyani a Monkey spunocytes; Agalu spanocytes; Canine spullecytes; Rat splenocytes; Mbewa splenocytes; MOSI SPLEOCHCES; Kalulu spullecytes; Splenocytes kudzipatula; Kuzizira spulenocyte; Kuthamangitsa spanocytes

Iphase Chopanga

Spleocytes (spls)Ndiwo chiwerengero chambiri cha ma cell a culeen, gulu lofunikira lokhudza kuseka magazi, kuyankha kwa mthupi la mthupi, ndi kusamalira homeostasis. Maselowa amaphatikizapo lymphocyte (ma cells, macolages), macrophages, maselo achilengedwe, ndi khungu lachilengedwe, omwe amagwiritsa ntchito chitetezo chovuta posinthasintha. Spulenycytes imagwiritsidwa ntchito kwambiri pamayendedwe opsinjika komanso omasulira kuti muphunzire chitetezo chamchiritso, kukula kwa katemera, kuwongolera matenda a autoimmune, ndi khansa ya sammunotherapy.

Udindo ndi kapangidwe ka spunocytes (spls)

Mkati mwa spuleen, spulekocytes imakhala kumadera ofiira: omwe amafalikira, omwe amasenda magazi ndikubwezeretsa maselo akale ofiira a m'magazi, ndi zamkati zoyera, komwe kuyankha kwamimba kumayambitsidwa. Zoyera zoyera zimakonzedwa mu maselo a T ndi B Makongeti apaderawa amapangitsa splenocyte (zopukutira) mwamphamvu mu Imminlogical Memss monga Elispot, wotulutsa cytometry, ndi kusakaniza lymphocyte.

Mitundu - Spulacytes (Spls)

-Cynomolgus spanocytes:Monnomolgus spanocytes amagwiritsidwa ntchito pofufuza zopitilira muyeso chifukwa amatha kuyankha mosamala kwambiri kwa munthu kapena kugwiritsa ntchito mornomolgus spakenocytes oyenera maphunziro apamwamba.

-Rasus monkey spanocytes:A Rusus Monkey spanocytes nawonso amafunika kufanana ndi zinthu zosiyanasiyana za ipingulogic yawo kwa anthu. Kafukufuku akugwiritsa ntchito ntchentche a ntchentche athandizira kutsika pakati pa mitundu ya zinyama ndi ntchito zamankhwala.

-Galu spanocytes / canine spanocytes:Pofufuza zowona, galu spanocytes (kapena canine spunecytes) amagwiritsidwa ntchito pophunzira zamitundu yazovuta mu zitini mu zitini komanso kujambulanso ndi mayankho amthupi.

-Kalulu spulekocytes: Kalulu spulecytes ndiofunika mu maphunziro a kupanga anti-popanga. Kalulu amathandizira kufotokozera zomwe zingasiyane ndi mbewa spanocytes kapena ralenocytes.

-Mbewa splenocytes / mbewa spanocytes:Makina a mbewa spanocytes ndi amodzi omwe amaphunziridwa pafupipafupi (spls) mu Immunology. Protocols ya kudzipatula splenocytes yochokera ku mbewa yakhazikitsidwa bwino, ndipo mbewa zonse ziwiri zimakhazikitsidwa ndi mbewa zomata zimagwiritsidwa ntchito potulutsa cytometry, elispot, ndi zina zomwe zimagwira ntchito.

-Rat spalecytes:Rat spalecytes amapereka chitsanzo chokwanira cha immunooticology ndi kaduka katemera. Rat spleocytes nthawi zambiri amayerekezedwa ndi mbewa splenocytes kuti amvetsetse kusiyana.

Splenocytes kudzipatula

Splenocytes kudzipatulaNdi njira yothetsera ma curcy curlen ku ndulu kuti igwiritsidwe ntchito pamapulogalamu osiyanasiyana, monga kutuluka kwa cytometry, ma cytokine opanga mawu, kapena kuphatikiza lymphocyte.

Spuween idachotsedwa pansi pa aseptic mikhalidwe ya iyo, pansi payotalikirana ndikusinthidwa ku chubu chosabala chomwe chili ndi RPMI 1640 sing'anga. Leukocyte wosanjikiza adasonkhanitsidwa mosamala pambuyo pa centrifugation. Maselo adatsukidwa ndi RPMI 1640 sing'anga, centrifuld ndipo sadatayidwa. Gawo lotsuka lidabwerezedwanso 1 - kawiri ma cell akhama omwe amagwiritsidwa ntchito poyesanso.

Kuzizira splenocytes

Kuzizira splenocytesndi gawo lofunikira kuti musunge maselo ogwiritsira ntchito mtsogolo. Kutulutsa kwa Crovorpe kumathandizira ofufuza kuti asunge spanocytes kwa nthawi yopitilira popanda kunyalanyaza zovuta zawo kapena magwiridwe antchito.

Mphamvu yayikulu ya kuyimitsidwa kwa chipata cha chinyezi kuchokera ku malowo kwa chisotidwe kumatayidwa ndipo khungu lidasokonekera. Onjezani ariquot ku chubu chilichonse chomasuka ndikusamukira ku chidebe chozizira, onetsetsani kuti maselo amakhalabe oyimitsidwa. Yambitsirani mwachangu zotengera zam'madzi mu - 80 ° C afirize. Tumizani ku - 150 ° C mzere wa freezer (kapena madzi a nayitrogeni) kwa nthawi yayitali - Kusungira nthawi yayitali.

Kuthamangitsa spanocytes

Kuthamangitsa spanocytesIyenera kuchitidwa mosamala kuonetsetsa kuchuluka kwa maselo am'madzi komanso magwiridwe antchito pambuyo pa Chropta. Njira yodumphira imathamanga kwambiri kuti muchepetse zovuta za DMSO ndi Ice Crystal.

Akuluakulu amasamutsidwa mpaka 37 ° C amasamba ndikusamba mpaka makristali abwino oundana otsala pang'ono. Onjezani 0,5 - 1ML ya Cell Smoenter kuti mugule chubu, kusinthitsa ndikusintha kuyimitsidwa ndi chubu cha 15ml kudzazidwa ndi khungu la cell. Centerrifuge, chotsani ndalama zapamwamba ndikujambula chubu kuti mumasule makonzedwe a cell. Onjezani 1ml ya chikhalidwe cha cell, kuwomba ndi kulola ndi pipette, onjezerani kukula kwa 15 ml. Centerrifuge, de - impanjire, onjezerani 1ML la chikhalidwe cha foni, kuwonjezera apakati malinga ndi kuchuluka kwa maselo. Ikani maselo mu chipilala cha CO2 ndikupanga 1h ndi kusiyana pang'ono pachikuto. Pamapeto pa makulitsidwe, kubwereka ndikusiyirani 1 kumine kuti mulole zinyalala zowonongeka. Kuyimitsidwa kwa maselo popanda kusunthika kunasinthidwa mosamalitsa ku zipatso zatsopano za 15ml. Maselo adawerengedwa kuti adziwe zochitika za maselo.

Mapeto

Spulecytes, ndi udindo wawo mosiyanasiyana ndi udindo wa mayankho amthupi, ndi zida zothandiza pakuchita kafukufuku. Ngakhale zimachokera ku makoswe, anyani, kapena mitundu ina ngati akalulu ndi agalu, spulnocytes amatsogolera maphunziro ovuta mu ntchito ya sayansi, njira ya katemera, ndi khansa ya Andnurotherapy. Njira zodzipatula, kuzizira, ndikuchepetsa ndikofunikira kuti musungidwe umphumphu wawo komanso kuthandizira ofufuzawo kuti awonetsetse ma cell amitundu osiyanasiyana. Monga kumvetsetsa kwathu kusambulu kumakulirakulira, kugwiritsa ntchito spulenocytes ipitilizabe kulimbikitsa kusiyana pakati pa kafukufuku wazomwe zili pakati pa maphunziro amtundu wambiri komanso kugwiritsa ntchito zinthu mwanzeru.