M'munda wa kafukufuku wa biomidal komanso kafukufuku, kumvetsetsa zigawo zam'manja ndikofunikira kuti musiye zovuta za ntchito yama cell ndi njira zamatenda. Zina mwa zigawo izi,tritosomes-Amachepera - Odziwika koma Ofunika Kalasi ya Lysosome - Zogwirizana ndi chisamaliro - makamaka molingana ndi mafumu.Rat tritosomesApatseni chidziwitso chofunikira mu enzymatic, matenda a metabolic, komanso m'badwo - zosintha zokhudzana ndi kusintha, kuwapangitsa kukhala mutu waukulu mu nthambi zoyeserera za zoyeserera.

Nkhaniyi ikuwunika zomwe ma tritosomes a ma tritosomes omwe ali okhawokha komanso amadziwika, ndipo chifukwa chake amafunikira kukafufuza kopita patsogolo pamadera osiyanasiyana.

Kuzindikira Tritosomes: Organelle Organelle

Tritosomes ndi subyaspe wa mathsosomes, omwe ndi nembanemba - Offganelles amakhudzidwa pakuwonongeka kwa macromolecule. Ngakhale ma lysosome okhazikika ali ndi ma enzymes a hydrolytic a acid a acid a acid ndi mawonekedwe apadera omwe amawasiyanitsa ndi anthu wamba a lysosomal.

Mawu oti "trinosome" adayamba kuyambitsa maphunziro omwe akufuna kuti agwirizane kwambiri pogwiritsa ntchito mitundu yosiyanasiyana komanso yosiyanasiyana. Mwa kugwiritsa ntchito njira zoterezi, asayansi adatha kuzindikira maulendo osokoneza bongo monga ma tritosomes, omwe amasiyana mu enzyme kapangidwe ka enzyme, kachulukidwe, ndi ntchito.

M'makoswe, ma tritosomes amaphunziridwa kwambiri chifukwa cha kupezeka kwa chiwalo - enzyme conners ndi cholembera chachikulu cha njira zopangira zigawo zaminyewa.

Kudzipatula komanso mawonekedwe a rat tritosomes

Kupatula kwa ma trat tritosomes kumaphatikizapo sucrose kapena perrifugletion yotsatira yotsatira minofu homogenization. Zinyama zomwe zimachitika zimakhala nthawi zambiri zimagwiritsidwa ntchito, monga hepatocytes zili ndi zitsamba zambiri komanso zofala zosiyanasiyana zam'madzi.

Njira zazikuluzikulu mu mawonekedwe a Tritosome zimaphatikizapo:

• Homogening rat chiwindi kapena minofu ya impso pansi pamagulu ozizira kuti isungire umphumphu.
• Kusinthanitsa kosiyanasiyana kuchotsa nuclei ndi Mitochondria.
• Kuchulukitsa kwa centrivent pertifogtion kudzipatula ma tritosomes kuchokera ku lysosomes wamba ndi ma vesi.
• Malingaliro a Enzyme kuti azindikire tritosome - Zochita zina, zomwe nthawi zambiri zimagwiritsa ntchito zolembera ngati β - glucuronidase, α - mannosidase, kapena archelfatase.

Kamodzinso, tritosomes amasanthula pogwiritsa ntchito ma microscopy ma microctopy, enzyme histonemistry, ndi njira zogwirira ntchito kuti muphunzire kapangidwe kake, kapangidwe kake, kapangidwe kake, ndi machitidwe ogwirira ntchito.

N 'chifukwa Chiyani Muyenera Kugwiritsa Ntchito Mitundu Yophunzira Tritosomes?

Makoswe ndichilengedwe omwe amakonda kwambiri pa ma cell ndi metabolic chifukwa chofanana ndi anthu, amangokhalira kugwiritsa ntchito bwino, komanso - adakhazikitsa ma labotale a labotale. Mphamvu ya rat, makamaka, ndi gwero lambiri la ma enzymes a lysosomal ndipo amakhala chitsanzo chodalirika pophunzira Endo - lysosomal Mphamvu.

Ma tritosomes a rat ndiwothandiza kwambiri pakufufuza

• Matenda a Lysosomal
• Ukalamba ndi misonecence
• Minofu - kagayidwe kambiri
• Mankhwala Osokoneza bongo ndi Kuopsa

Kuchulukitsa kwa ma tritosomes oyambira minofu ya rat yawapangitsa kukhala ndi chitsanzo cha maziko a Lyshosomal heterogeneity, chomwe chikuwoneka ngati chovuta kwambiri m'matenda ambiri matenda osachiritsika.

Mapulogalamu a Maphunziro a Jut Tritosome mu kufufuza kwa Biomedal

1. Mavuto osungira (LSDS)

Ma tritosomes amapatsidwa michere inayake yomwe ili yopanda tanthauzo kapena yopanda pake mu LSDS. Kuwerenga izi organelles kumathandizira EPEMVE POPHUNZITSIRA MALO OGWIRITSIRA NTCHITO NDIPONSO ZOPEREKA.

Mwachitsanzo, ofufuza agwiritsa ntchito ma tritosomes kumvetsetsa njira zodziwika bwino ku Gaurya ndi Tays - Matenda a Sachs, amazindikira matenda omwe amatha kuchita zochizira zinthu zina.

2. Akalamba ndi Autophakhul Agerge

M'badwo - Zosintha zokhudzana ndi Lysosomal ntchito ndi chizindikiro cha ma cell okalamba. Kafukufuku wa Tritosome mu makoswe okalamba awululira za lipofuscin, enzyme ntchito, komanso kuwonongeka kwa autophagy-malo ofunikira a maselo a Senernt.

Izi zimapangitsa ma tritosomes chida chamtengo wapatali chofufuzira Zaka - Njira Zosasinthika, kuphatikizapo omwe amalumikizidwa ndi neuroodgeneration ndi kuwononga minofu.

3. Toxicology ndi pharmacokinetics

Lysosomes, kuphatikiza tritosomes, amatenga nawo mbali munjira yopumira ndi kuwonongeka kwa ma Xenobiotic. Pophunzira tritosomes, akatswiri oopsa amatha kumvetsetsa momwe Mankhwala osokoneza bongo ndi poizoni zachilengedwe zimakonzedwa ku ma cellular.

Mbiri ya Enzyme Zochitika mu tritosomes imathanso kuthandizanso kulosera ngati kagayidwe ka mankhwala, mankhwala osokoneza bongo, kuchirikiza chitukuko cha mankhwala a Saccocrooloraologiology.

4. Kuyerekeza za cell

Ma tritosomes a rat amalola ofufuza kuti afananitse mkhalidwe wa lysosomal mitundu ndi minyewa. Izi ndizothandiza kwambiri mukamaphunzira mitundu - enzyme knetics, gawo la subcellulation, ndi metabolic kutero.

Njira zowunikira zomwe zimagwiritsidwa ntchito mu kafukufuku wa Tritosome

Phunziro la rat tritrosomes limagwiritsa ntchito njira yophatikizira njira yamakono komanso yamakono:

• Enzyme ntchito ya enzymes ya kusanthula kwa ma hydrolase.
• Kufalikira kwa Western komanso Spectrometry ya chizindikiritso cha mapuloteni.
• Immunoorescence ndi microcalcopy yosokoneza maphunziro.
• Lipidomecs kuti muyenetse nyimbo za membrane ndi malo osungira.

Zida izi zimathandiza ofufuza kuti atulutse maudindo a ma tritosomes mu cell nthochi ndi pathophology moyenera.

Zoperewera komanso malingaliro amtsogolo

Pomwe kafukufuku wa rat amafufuza kwapereka kuzindikira kwakukulu, zoperewera zingapo zikutsalira:

• Kumasulira heysosomes kumatha kutseka kumasulira kwa zotsatira zapansi.
• Mitundu - Kusiyana kwapadera kuyenera kuganiziridwa mukamasinthanitsa pa biology ya anthu.
• Kukhazikika kwa ma protocols ndikofunikira kuti zithandizire kubereka pamilandu.

Ngakhale pali zovuta izi, chidwi cha ma lysosome am'mimba ngati ma tritosomes akukula, makamaka kagayidwe ka kagayidwe, ndi magetsi olakwika. Kafukufuku wamtsogolo amatha kuyang'ana kwambiri popanga ma fluorescers kapena moyo - njira zofananira kuti muphunzire mphamvu ya tritrosomal munthawi yeniyeni.

Mapeto

Ma tratrosomes amaimira mawonekedwe apadera komanso othandiza ma cell omwe amagwira ntchito yofunika kwambiri mu zyosomal biology. Mwa kuluka ndi kuphatikizira izi, ofufuza athe kukulitsa kumvetsetsa kwawo kagayidwe kagayidwe, m'badwo - Zokhudza Kufanananiza Kumachitika.

Monga momwe anthu asayansi amapitilizabe kudziwa zovuta zam'mimbazi, ma tritosomes a rat amakhala ndi mtundu wamtengo wapatali pochotsa zovuta za njira zamiyondo ndi matenda.