Kupita patsogolo ku Bioanalysis: Udindo wa LC - Ms / Ms Mu Ophthalmic Develod ndi Maphunziro am'madzi am'madzi

Zinthu za ifase

Madzi a chromatography - tandem misa spectrometry (LC - MS / MS)

Madzi a chromatography - tandem misa spectrometry (LC - ms / ms) Ndi njira yowunikira yowunikira yomwe imaphatikiza kuthekera kwa madzi amadzimadzi a chromatography ndi luso la kusanthula kwa tandem misa spectmetry. Mu LC - MS / MS, osakaniza amalekanitsidwa koyamba ndi ma chromatography, pomwe zigawo zikuluzikulu zimalumikizana mosiyanasiyana ndi gawo lakumanja ndi gawo la tele station. Zigawo zopatukana zimadziwika ndikusanthula ndi tandem misa spectrametry, zomwe zidutswazo zing'onozing'ono zimaphatikizidwa ndi ma ion.

Mapulogalamu a LC - MS / Ms ku Bionalysis

Bioanalysiszimaphatikizapo kukula kwa mankhwala osokoneza bongo, metabolites, ndi zina zopangira zachilengedwe malinga ndi zitsanzo za zitsanzo, magazi, ma plasma, mkodzo, ndi zinama biofluids. LC - MS / MS ili bwino - oyenerera ntchito izi chifukwa cha chidwi chake ndi kuthekera kozindikira zinthu zosanja zomwe zingachitike ma santeliological matties.

LC - Test ukadaulo wa MS / MS Posanthula zitsanzo zazomwe zimawonjezera zinthu zonse komanso za endogenose. Ofufuzawo adapanga zitsanzo zenizeni powonjezera chinthucho kuti ayesedwe kwa aMatrix opanda kanthukupanga zitsanzo zochulukirapo komanso zitsanzo zowongolera. Kukhazikika kwa chinthucho kuti ayesedwe muyezo wocheperako kumangokhala ndi curve wokhazikika.

Zinthu zachikhalidwe ndi zinthu zomwe zimachitika mwachilengedwe mthupi. Sometorous chinthu - mankhwala okhudzana ndi a Endos akhala malangizo ofunikira pakupanga mankhwala atsopano m'zaka zaposachedwa. Pamodzi ndi kubadwa kwa kuchuluka kwa mankhwala osokoneza bongo omwe ali ndi zinthu za endo native, baonalyssis mankhwala omwe ali ndi zinthu za endotogenoses tsopano ndiofunika kwambiri. Komabe, pakalipano, kutsimikizika kwa njira zachitsanzo zokusakanikirana mwa FDA ndi mabungwe ena apanyumba komanso akunja makamaka makamaka amayang'ana kwambiri zinthu zochulukirapo, kuphatikizapo kulondola, kulondola, kukonza, komanso kukhazikika. Popeza kupezeka kwa zinthu za Endotogenous kumabweretsa mavuto mu zotsatira za zojambula chifukwa chake mukamapeza matrix osatengera matrix enieni, kutuluka kwa njira inaMatleological Matrix (Matrix opanda kanthu) Muthambitse vutoli.

Gome 1: Kufotokozera za kusankhidwa m'makampani a Bionalytical Njira Yotsimikizika Yotsimikizika

Kusanthula kwa kafukufuku ndi kutsimikizika kwa njira yotsimikizika

Ku Bioanalysis, kuonetsetsa kudalirika komanso kubereka kwa zotsatira zowunikira ndikofunikira. Izi zimafuna chitukuko champhamvu ndipokutsimikizika kwa zowunikiraNjira.

Kukula Kwa Njiraimaphatikizapo kupanga njira zotheka kuzindikira ndi kuchuluka kwa chidwi. Izi zikuphatikiza kusankha malo oyenera a chromatographic (E.g. Kuphatikiza apo, njirayi iyenera kukhala yothetsera zowunikira mosavuta pamaso pa matricts osasinthika komanso osinthika omwe nthawi zambiri amapangidwa ndi mapuloteni, lipids, ndi zina zomwe zingasokoneze kusanthula.

Njira ikayamba, iyenera kuchitikaKutsimikizika kwa mawonekedwekuwonetsetsa kuti ikukumana ndi njira zopangidwira. Njira yotsimikizika iyi ndiyofunikira kuti mutsimikizire kuti njirayi ndiyoyenera pazolinga zake ndikugwirizana ndi zofunikira zowongolera. Njira za bioinalytical, zovomerezeka nthawi zambiri zimaphatikizapo magawo angapo ofunikira:

• - Kulondola ndi Kulondola:Kuonetsetsa kuti njirayo imapereka zotsatira zolondola komanso zosasinthasintha.
• - Kukhuzidwa:Kuthekera kuona zowunikira zoyeserera.
• - Kusankha:Kutha kwa njirayo kusiyanitsa kusanthula kwina kwa mankhwala ena mu matrix.
• - Kubwezeretsa:Kuchita bwino komwe kafukufukuyu amachotsedwa ku zitsanzo zachilengedwe.
• - Kukhazikika:Kukhazikika kwa kafukufuku wosungirako mosiyanasiyana komanso kusamalira zinthu.
• -Kutha kwa njira kupangira zotsatira zomwe zili molingana mwachindunji ndi kusanthula kwa oyang'anira pa mtundu umodzi.

Matlelogical matrix ndi opanda kanthu matrix amasewera maudindo ofunikira mu izi. Zitsanzo za kuwongolera, zomwe sizikugwirizana ndi chidwi, ndizofunikira kuti zidziwitse zotsatira zomwe zasanthuli kapena zosemphana ndi kuwunika. Amathandizira kukhazikitsa maofesi am'munsi kuti awerengere ndikuwonetsetsa kuti matrix pawokha samathandizira kuipitsidwa kapena kuponderezana. Momwemonso, kugwiritsa ntchitoMankhwala - Matrices AulereNdikofunikira kuti mutsimikizire kuti palibe mankhwala otsala kapena ma metabolites omwe alipo mu zitsanzo zomwe zingapangitse zotsatira zake.

Bioanalysis a mankhwala ophthalmic

Khoma la diso la diso lagawidwa m'magawo atatu, khoma lakunja ndi ma nebuous nembanemba; Middlene wapakati ndi nembanemba, vasclar nembanemba kapena uvea; Ndipo membrane wamkati ndi retina. Maso amaso amagawidwa magawo awiri, madera achimphepo komanso oyambira m'maso, omangidwa kumbuyo kwa mandala.

Chithunzi 1. Maso a diso la munthu.

Magulu akuluakulu omwe amakhudzidwa ndi mankhwala osokoneza bongo amaphatikizapo:

• Kuchuluka- The Tsitsi loyamba la mankhwala otsekemera, okhala ndi ma encrase ndi cytochrome p450 (cyp) michere yomwe imapatsa ma prodrugs.
• Conjunctiva- Olemera mu mankhwala - michere yopukusira (E.g.
• Nthabwala zamadzi- Zochita zosagawika kagayidwe koma zimadya mankhwala osokoneza bongo komanso chilolezo.
• Mtedza- Intravitreal jakisoni amatha kuchita mwachindunji pa retina ndikuchepetsa zoopsa m'mayendedwe. Mankhwala ang'onoang'ono a Molecule amasuntha mwachangu, ndipo mankhwala akuluakulu a Molecule ali ndi theka lalitali - Moyo. Kusintha kwamphamvu ndi ukalamba kumakhudzanso pharmacokinetics.
• Sclera- Sclera imawoneka bwino kwambiri kwa mankhwala akuluakulu a molekyu ndi vesi la mankhwala kudzera pa sclera limakhudzidwa ndi kukula kwazomwe zimakhudzidwa. Ma jekeseni a Susconunctival amalola mankhwala kuti alowetse choroid, koma njirayi ndiyovuta. Scleral Melanin imamangiriza mankhwalawa ndipo imakhudza kutulutsidwa ndi nthawi yayitali.
• Dera loyang'ana maso- Matenda otumphuka amakhala olemera m'magazi ndi mankhwala osokoneza bongo atha kuchotsedwa kudzera mu kufalikira kwa thupi kapena lymph. Choronal cypecular hyperpermeilical amalola mankhwala kuti alowetse malo akunja, koma ndizovuta kuwoloka khungu la epithelilium, lomwe limakhudza kuwonongeka. Melanin - Mankhwala omanga mankhwala amatha kupitirira kutalika kwa zochita.

Nthabwala zamadzi komanso nthabwala

Anthabwala zamadzindiFrondus FrorMafuta ofunikira ofunikira omwe amasewera maudindo ofunikira popewa kupanikizika kwa ma intraocular, kupereka michere, komanso kuwongolera mawu owoneka bwino. Chiwongola dzanja kwambiri ndi madzi oonda, omveka, amadzi omwe amadzaza zipinda zam'mbali zonse za diso, zomwe zili m'maso, okhala ndi ma ions, mapuloteni, chakudya, ndi mpweya wabwino. Ambiri mwa nthabwala zowoneka bwino zopangidwa ndi Tiliary Thupi, diso kumangopangidwa ndi gawo la iris ndi Cornea. Madzi awa amasiyanasiyana mitundu ya mitundu, kuphatikiza anthu, anyaluta, akalulu, ndi ena omwe si - Arges. Nthawi zambiri amatenga mbali zambiri kuchokera ku nyama kapena ziweto.

Nthabwala zamadzimadzi

Nthabwala zamunthu

Anthabwala zamunthundi chomveka bwino, chambiri - madzi olemera omwe amasuntha mpweya wabwino komanso umachirikiza ntchito za mbozi za majerembali ndi mandala. Imapangidwa ndi Thupi la Cilialo ndikuyenda kudzera m'chipinda chopanda kumbuyo musanakwere mankhwala osokoneza bongo.

Moto Wochita nthabwala

AMoto Wochita nthabwalaKufanana kwambiri ndi anthu omwe akuphatikizidwa ndi mphamvu. Popeza kufanana kwa anatomiadicasti zinthu pakati pa anyani ndi anthu,NAMO - Oseketsa AmkondaAmakhala ngati ofunikira pa maphunziro a ophthalmic.

Nthabwala zam'madzi

Anthabwala zam'madzizimasiyana kwambiri kuchokera kwa anyani, makamaka mu mapuloteni ake komanso kuchuluka kwa zosintha. Akalulu amagwiritsidwa ntchito pofufuza za ocular, ngakhale mitundu - Kusinthana kwatsatanetsatane kuyenera kuganiziridwa.

Masewera a Vitaus Mitundu

Nthabwala zaumunthu

Anthabwala zaumunthundi gel a gel - Monga zofunikira zopangidwa makamaka ndi madzi, collagen, ndi hyororonic acid. Zimakhala zowoneka bwino, zimatenga zikhumbo, ndipo zimagwira ngati njira yoyendera michere.

Nthabwala za Monkey

Anthabwala za MonkeyAmagawana chimodzimodzi ndi nthabwala zamphamvu za anthu, zopangaNjokaCHITSANZO chamtengo wapatali chophunzirira!

Nthabwala za kalulu

Anthabwala za kalulundizosiyana ndi zopangidwa mosiyanasiyana, kukhala zochulukirapo - Ndimakonda komanso kukhala ndi kachulukidwe kotsika. Kusiyanaku kumathandizanso kuyankha kwa opaleshoni ya opaleshoni ya opaleshoni ya zamankhwala.

Kukula kwa madzi opanga maonjedwe

Madzi am'madzi ndi oyendayenda

Nthabwala zonyozandikungochita phokosoOfesi yopanga mainjini yopangidwa kuti igwiritse ntchito maopaleshoni owopsa, kutumiza mankhwala osokoneza bongo, komanso kugwiritsa ntchito mapulogalamu. Madzi opanga awa amatsamba zazomwe zimadziwika bwino.

Zoyeserera zowoneka bwino komanso zowoneka bwino

Zosangalatsa ZoseketsandiMantha aulukandi labotale - Mayankho okonzekera omwe amagwiritsidwa ntchito poyeserera ku Vitro ndikuwonetsa zojambula za ocular. Amathandizira maphunziro olamulidwa popanda zovuta zomwe zimagwirizanitsidwa ndi zitsanzo za nyama kapena za anthu.

Mapeto

Kugwiritsa ntchito mankhwala a chromatography - tandem misa spectometry (LC - MS / MS) ku Bioanalysis akuimira njira zowunikira kuti azindikire ma mecrocalogical matrict. Njira Yabwino Kwambiri, yolondola, ndipo kusankha kumapangitsa kukhala kofunikira kwambiri pakuwunika kwa exougesoniction, makamaka mu ophthalmic kukula. Kumvetsetsa mwatsatanetsatane kwa oculamy ndi gawo lamadzi monga momwe amawonera kwambiri komanso kuwononga nthabwala zomwe zimapangitsa kuti zigawo zigawo zikapangidwe. Kuphatikiza apo, chitukuko cha madzi opanga komanso chowoneka bwino chimapitilira pamene tikuwonetsetsa kuti malingaliro amakwaniritsidwa. Monga kuwunika kwa njira ikupitilira kusinthika, kumatsimikizira kudalirika komanso kubereka kofunikira pakugwiritsa ntchito mankhwala azachipatala, makamaka mu ophthalmology.

Mawu osakira: LC - MS / MS, matrix osavomerezeka, oseketsa, nthabwala zamankhwala, nthabwala zamunthu, nthabwala zamunthu, Hartard nthabwala, zopanda - - zamunthu wambiri zaumoyo,Woyesedwa wowoneka bwino kwambiri, nthabwala kwambiri, nthabwala zonyoza, nthabwala zonyoza, nthabwala zongopeka.

Kuchulidwa

SeydPedour, S. M., igchaight, L., Rezazide, G., & Ricken, T. (2023). Mtundu wa masamu wa kuyankha kwamphamvu kwa glaucoma yokhazikika.Muyeso: masensa, 30, 100936. Https:/,doi.orgrr .10.1016/J.Measen.2023.1009336