Mfundo ya Comet Assay
Comet Assay, yomwe imadziwikanso kuti single-cell gel electrophoresis, ndi njira yodziwira genotoxicity pozindikira kuwonongeka kwa DNA strand. Imatha kuzindikira bwino ndikuwunika kuchuluka kwa kuwonongeka kwa DNA imodzi komanso yachiwiri yachingwe m'maselo. Pamene zosiyanasiyana amkati ndi exogenous DNA kuwonongeka zinthu kuchititsa ma DNA chingwe yopuma, kapangidwe ake superhelical kuonongeka, ndipo pansi pa zochita za selo lysate, nembanemba selo, nembanemba nyukiliya ndi zina nembanemba kapangidwe ndi kuonongeka, ndi okhudza maselo ambiri mapuloteni, RNA, ndi zigawo zina diffus mu selo lysate, pamene nyukiliya kukhalabe DNA yaikulu m'malo chabe kukhala nyukiliya DNA.M'malo osalowerera ndale, zidutswa za DNA zimatha kulowa mu gel ndikusuntha, pomwe pansi pa zochita za alkaline electrolyte, DNA imachotsedwa ndikuwonongeka kwa DNA yosweka zingwe ndi zidutswa zimatulutsidwa. Chifukwa cha kachulukidwe kakang'ono ka ma DNAwa komanso kusinthika kwa alkaline kukhala chingwe chimodzi, ma DNA oyipa amasiya DNA ya nyukiliya kupita kumtengo wabwino panthawi ya electrophoresis, zomwe zimapangitsa chithunzi chofanana, pomwe magawo osawonongeka a DNA amakhalabe ozungulira. Choncho, ikhoza kugawidwa m'magulu amtundu wa comet test (PH = 8.4) ndi alkaline comet test (PH> 13) malinga ndi kusiyana kwa pH kwa electrophoresis buffer. Zakale zimagwiritsidwa ntchito kwambiri kuti zizindikire kuwonongeka kwa DNA pawiri-zingwe zowonongeka, pamene zotsirizirazi zimakhala ndi mphamvu zambiri ndipo zimatha kugwiritsidwa ntchito kuti zizindikire kuwonongeka kochepa kwa single-strand and double-strand breakage.
Kaŵirikaŵiri, pamene DNA imawonongeka kwambiri, m’pamenenso chingwe ndi zidutswa za zidutswa zimaduka kwambiri, n’kukhala zing’onozing’ono m’litali mwake, m’pamenenso DNA imasamuka m’mikhalidwe yofanana ya electrophoretic, ndi mtunda wautali wa kusamuka kwake. Chifukwa chake, kuchuluka kwa kuwonongeka kwa DNA m'maselo amodzi kumatha kuzindikirika poyesa kuchuluka kwa kuwala kapena kutalika kwa gawo lomwe lasamutsidwa la DNA, potero kudziwa kugwirizana pakati pa mlingo wochitapo kanthu wa mutuwo ndi kuwonongeka kwa DNA.
IPHASE Products
Kusavuta: The reagents ndi consumables zofunika single-selo gel osakaniza electrophoresis amaperekedwa ndipo angagwiritsidwe ntchito mwachindunji, kwambiri kufupikitsa mkombero kuyesera.Kulondola: Chigawo chilichonse cha zidachi chayesedwa mwamphamvu kwambiri, ndipo zotsatira zoyeserera ndi zolondola, zodalirika komanso zobwereketsa kwambiri.
Kukhazikika: chidacho ndi chokhazikika komanso chosavuta kunyamula ndikusungidwa.
Kugwiritsa ntchito kwambiri: Ili ndi ntchito zambiri ndipo imatha kugwiritsidwa ntchito poyesa genetic toxicology pazakudya, mankhwala, mankhwala ophera tizilombo, opha tizilombo, zowonjezera zakudya, zodzola, zida zamankhwala ndi zina.
IPHASE yakhala ikuchita nawo ntchito zowunikira kafukufuku wa sayansi kwa zaka zingapo, ndipo yapanga mndandanda wa zida zofufuzira zasayansi zam'mbali - malo ndi mitundu yosiyanasiyana - IPHASE imapereka zida zowunikira zolondola komanso zogwira mtima kuti zithandizire kubadwa kwa mankhwala atsopano; ilinso nyenyezi yowala mu malo akuluakulu ofufuza za sayansi ya moyo, nthawi zonse imapatsa asayansi zipangizo zatsopano, njira zatsopano ndi malingaliro atsopano, zomwe zimatsogolera kudumpha kulikonse m'malire a kafukufuku wa sayansi. M'bwalo lamakono la kafukufuku wa genotoxicity, IPHASE yawonetsa luntha lodabwitsa ndi kupha, ndikuyambitsa njira zingapo zosavuta komanso zogwira mtima kuti kafukufuku wovuta azitha kuyenda bwino komanso wogwira ntchito.
Nthawi yotumiza: 2024 - 07 - 26 15:29:08