Nhanganyaya kune Rat Livery S9
Rat chiropa s9iri sub - cellular chidimbu chakawanikwa kubva kune chiropa tishu, kunyanya kubva kumakonzo, zvichitevera homogenization uye centrifugation maitiro. Iyo ine musanganiswa we enzymes uye cofgers kubva kune ese microsomal ne cytosolic zvidimbu zvechiropa chemaromo. RATL LIME S9 inobata kukosha kukuru mune biochemical uye pharmacological kutsvagisa basa rayo mukuremerwa muVivo muVivo Metabolism. Vatsvagiri vanoshandisa rat chiropa s9 enzymes yekudzidza zvinodhaka metabolism, zvakatipoteredza nehupfumi, uye biochemical nzira dzakabatanidzwa mukuita uye detoxication yezvinhu zvakasiyana siyana.
Biochemical zvikamu zve rat livers s9 |
Enzymatic chimiro |
RATL LIME S9 akapfuma mune dzakasiyana siyana enzymes iyo inotora chikamu mune zvese zvikamu ini uye chikamu Ii chei metabolic maitiro. Iyi yakaratidza enzymatic mbiri inotendera iyo reti reti remuziyo s9 chidimbu chekuita semhando yekubira yekufunda zvinodhaka nzira. |
Metabolic nzira inobatanidzwa |
Iyo metabolic nzira yakadururwa neye rat chiropa s9 Chiitiko chakasanganiswa cheiyi nzira inobatsira kudzikisira Xenobiotics XENObiotic uye shandura prodrugs mune yavo inoshanda mafomu. | |
Kugadzirira uye kuongororwa kwe rat chiropa s9 |
Laboratity maitiro |
Kazhinji inoshandiswa buffers inosanganisira Tris - HCL kana potasasos phosphate, kazhinji inowedzerwa necficor seNdph kutsigira enzyme basa. Kuchengeta chiropa tishu pane imwe tembiricha yekupisa mukati mekuita kwakakosha kuchengetedza enzymatic basa. |
Zvishandiso |
Toicology zvidzidzo |
Zvinodhaka metabolism:Mu vitro assays kushandisa rat chiropa chivharo S9 inogona kufanotaura metabolic kugadzikana uye kugona kugona kweshoko retsiva, nekudaro kusarudza sarudzo panguva yekubudirira zvinodhaka. |
| Carcinogenicity kuyedza:Chikamu cheS9 chinowanzowedzerwa ku muutagenicity bvunzo senge maAmes bvunzo, iyo inoongorora maitiro eComerwa mumuviri inokonzerwa nekuwonekwa kwemakemikari. | ||
Pharmacokinetics |
Phase i uye chikamu II chekuita:Pharmacokinetics zvakanyanya kuvimba nekukasira kugona kweiyo rat chiropa S9. Iyo Fratt's REPR YEMAHARA yeNzymatic inobvumira vaongorori kuti vadzidze zvese zvikamu zvese ini uye chikamui II neChikamu cheI neChikamu munzvimbo yakadzorwa. | |
| Zvinorehwa nekugadzirwa kwezvinodhaka:Nekuzivisa metabolic hotspots uye angave ane muchetura wepachuru mukushandiswa kwezvinodhaka | ||
Kuyedza makemikari emagariro |
Xenobiotic metabolism:RATL LIME S9 ishandiso yakakosha yekufunda metabolism yemakemikari emamiriro ezvinhu, anozivikanwa seXenobiotic. Aya makomisheni, ayo anosanganisira zvipikisi zvemishonga, maindasitiri, uye kusvibisa, zvinogona kuitika biotransformation yakakura muchiropa. Iwo ma enzymes aripo muRat Livers S9 inogona kutevedzera metabolic maitiro ayo aya Xenobiotic agonetsio mu vivo, kupa ruzivo rwekuziva kuita kwavo kwekukanganisa uye nemamiriro ekunze. | |
| Yakatipoteredza njodzi kuongorora:Nekuongorora kuti makemikari ezvemamiriro ekunze anoitwa nemakemikirwo emakekitirwo ezvinhu |
Zvinonetsa uye Rudzidziso
Kugumira uye Matambudziko |
Kusiyanisa mune enzyme chiitiko |
Chimwe chezvinetso chikuru mukushandisa rat chiropa s9 ndiyo kusiyana-siyana muEzzyme chiitiko pakati pekugadzirira kwakasiyana. Iyi misiyano inogona kumuka kubva mumisiyano mukunyanyisa protocol, hutano uye zera remhuka, uye zvakananga madhiri akashandiswa. |
Matenganiso ehunyanzvi uye kugadzirisa matambudziko |
Zvakawanda zvehunyanzvi zvehunyanzvi zvinogona kukanganisa kushandiswa kwakabudirira kwe rat chiropa s9 mukutsvaga. Kuchengeta chiitiko che enzymatic panguva yekuchengetera uye kubata kwakakosha, sezvazvakaita kuti kuve nemamiriro ezvinhu akakodzera ekuedza muVitro assays. Vanotsvagurudza vanofanirwa kuve vanogashira pakutambudzika nyaya zvine chekuita ne enzyme kugadzikana, kuuraya mamiriro, uye kududzirwa kwedata. Kukurira matambudziko aya kunoda musanganiswa wehunyanzvi hwehunyanzvi uye kukwirira - zvigadzirwa zvemhando yepamusoro, sekuti izvo zvinopihwa neyechikamu chakanyatsogadzirirwa River Vagadziri veS9 | |
Kufambira mberi kwazvino uye zvigadzirwa |
Nzira Nyowani |
Dzazvino kufambira mberi mukugadzirira uye kushandiswa kwe rat chiropa s9 zvakavandudza zvakanyanya zvekushandisa mukutsvaga. Kugadzirwa kunosanganisira kuvandudzwa kwekubudirira kwehunyanzvi hwekunzwisisa kuti uwane yepamusoro enzyme mazwi ekutaura uye kushandiswa kwehunyanzvi hwekuongorora maitiro kuti azotarisa chiitiko che enzyme zvakanyanya. |
Tekinorology Kuvandudzwa muS9 Analysis |
Tekinoroji yekuvandudzwa, yakadai seyakakwirira - resolution misa spectrometry uye inotevera - kuyananiswa kwerudzi, ndazvidzokorora kuongororwa kwe metabolic nzira yakadururwa ne rat chiropa s9. Aya matekinoroji anotendera kuti udzidze hunhu hwe metabolic antermemedies uye kuzivikanwa kweNovel metabolic nzira. |
Kutumira nguva: 2024 - 08 - 19 13: 47:58