Principle
IPHASE'sPBMC yekuzviparadzanisa nevamwe kits yakavakirwa paiyo classical density gradient centrifugation nzira, iyo yakavakirwa pamusiyano wekugadzirisa kumhanya kwemaseru eropa akasiyana mune density gradient yepakati kuti uwane kupatsanurwa kwesero. Inosanganisirwa mukiti ndeye yakanyatso kugadzirirwa density gradient kupatsanura mhinduro inogadzira yakagadzikana density gradient panguva yecentrifugation. Kana iyo anticoagulated yese yeropa sampu inowedzerwa zvishoma nezvishoma pamusoro pemhinduro yekuparadzanisa, masero matsvuku akadai semasero matsvuku eropa uye granulocytes anogara kuzasi kwe centrifuge chubhu, nepo maPBMC asina kuomesesa anomiswa pamushonga pakati pemhinduro yekuparadzanisa uye plasma, achigadzira akajeka, machena, makore ane makore emasero. Nekunyatso kuunganidza iyi layer yemasero uye kunotevera kugezwa uye centrifugation, yakakwirira kuchena PBMC inogona kuwanikwa.Sumo pfupi
IPHASE's PBMC Isolation Kit chishandiso chakagadzirirwa vaongorori kuti vanyatso patsanura PBMC kubva kune anticoagulated ropa rose. Iyo kit ine zvinotevera maitiro:1.Kuparadzana kwakanyanya kunobudirira: kushandiswa kwekugadzirisa density gradient separator kunovimbisa kuparadzaniswa kwakakurumidza uye kwakarurama kwePBMC panguva ye centrifugation, nekuderedza kusvibiswa kubva kune mamwe marudzi emasero.
2.Kuchena kwepamusoro: kuburikidza nehutano hwakanaka hwekuparadzanisa uye matanho anotevera ekugeza, kuchena kwepamusoro kwePBMC kunogona kuwanikwa, kupa hwaro hwakavimbika kune hunotevera sero tsika, molecular biology experiments nezvimwe zvakadaro.
3.Sterility uye yakaderera endotoxin: mhinduro yekuzviparadzanisa uye zvimwe zvikamu mukiti zvakaiswa pasi pekurapa kweaseptic yakasimba uye yakanyanya kuderera endotoxin mazinga, kuve nechokwadi kuti hapana kusvibiswa kusingadiwi kuchaunzwa panguva yekuzviparadzanisa nevamwe.
4.Zvakawanda zvinoshanda: kwete chete yakakodzera kuparadzaniswa kwePBMC yevanhu, asiwo inopa PBMC yekuzviparadzanisa kits kune dzimwe mhuka dzemhuka (dzakadai setsoko, imbwa, rat, mouse, nezvimwewo) kuti isangane nezvido zvakasiyana-siyana zvekutsvakurudza.
5.Easy kushanda: Kiti yakarongedzerwa nemirairo yekushanda yakadzama uye maturusi anodiwa ekubatsira, izvo zvinoita kuti nzira yese yekuzviparadzanisa ive nyore uye nyore, kunyangwe kune vanotanga kukurumidza kutanga.
Mienzaniso yekushandisa
IPHASE's PBMC yekuzviparadzanisa kits yakashandiswa zvakanyanya mumhando dzakasiyana dzebiomedical research. Semuyenzaniso, mu immunology tsvakurudzo, vatsvakurudzi vanogona kushandisa akaparadzana PBMC yokuronga uye kushanda kudzidza masero T, B masero uye mamwe masero immune; mukutsvagisa oncology, PBMC inogona kushandiswa kuongorora mamiriro ekudzivirira ezvirwere zvebundu uye screen anti - tumor immune cell. Pamusoro pezvo, kititi chinoshandiswa zvakanyanya mukuvandudza vaccine, kuongorora zvinodhaka uye mimwe minda.Mhedziso
IPHASE's PBMC yekuzviparadzanisa nevamwe kit yakatora chinzvimbo chakakosha mumunda wekutsvagisa biomedical nekuita kwayo kwakanaka uye kukosha kwekushandisa kwakakura. Nekuenderera mberi kuri kuita sainzi uye tekinoroji uye kuwedzera kuri kudiwa kwekutsvaga, IPHASE icharamba ichizvipira mukugadzira zvinhu zvinonyanya kushanda, zvakachena uye zviri nyore-kushandisa zvipenyu zvebiological reagent kupa rutsigiro rwakasimba kune vanoongorora.Nguva yekutumira: 2024-07-27 16:30:36